Reviewed,
UniProtKB/Swiss-Prot P01074 (ITR1_CUCMA)
Last modified
July 22, 2008.
Version 64.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Trypsin inhibitor 1 Alternative name(s): Trypsin inhibitor I CMTI-I ITD-I |
| Organism | Cucurbita maxima (Pumpkin) (Winter squash) |
| Taxonomic identifier | 3661 [NCBI] |
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Cucurbita |
Protein attributes
| Sequence length | 29 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Inhibits trypsin. |
| Subcellular location | |
| Domain | The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin. |
| Sequence similarities | Belongs to the protease inhibitor I7 (squash-type serine protease inhibitor) family. [View classification] |
Ontologies
Keywords | |
|---|---|
| Cellular component | Secreted |
| Domain | Knottin |
| Molecular function | Protease inhibitor Serine protease inhibitor |
| Technical term | 3D-structure Direct protein sequencing |
Gene Ontology (GO) | |
| None. [Check GOA] | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | ||||||||
Molecule processing | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Peptide | 1 – 29 | 29 | Trypsin inhibitor 1 | |||||||||
Sites | ||||||||||||
| Site | 5 – 6 | 2 | Reactive bond | |||||||||
Amino acid modifications | ||||||||||||
| Disulfide bond | 3 ↔ 20 | |||||||||||
| Disulfide bond | 10 ↔ 22 | |||||||||||
| Disulfide bond | 16 ↔ 28 | |||||||||||
Secondary structure | ||||||||||||
Helix Strand Turn | ||||||||||||
| Helix | 13 – 15 | 3 | ||||||||||
| Beta strand | 26 – 28 | 3 | ||||||||||
Sequences
References
| [1] | "Amino-acid sequence of two trypsin isoinhibitors, ITD I and ITD III from squash seeds (Cucurbita maxima)." Wilusz T., Wieczorek M., Polanowski A., Denton A., Cook J., Laskowski M. Jr. Hoppe-Seyler's Z. Physiol. Chem. 364:93-95(1983) [PubMed: 6840699] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Seed. |
| [2] | "The refined 2.0 A X-ray crystal structure of the complex formed between bovine beta-trypsin and CMTI-I, a trypsin inhibitor from squash seeds (Cucurbita maxima). Topological similarity of the squash seed inhibitors with the carboxypeptidase A inhibitor from potatoes." Bode W., Greyling H.J., Huber R., Otlewski J., Wilusz T. FEBS Lett. 242:285-292(1989) [PubMed: 2914611] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). |
| [3] | "Determination of the complete three-dimensional structure of the trypsin inhibitor from squash seeds in aqueous solution by nuclear magnetic resonance and a combination of distance geometry and dynamical simulated annealing." Holak T.A., Gondol D., Otlewski J., Wilusz T. J. Mol. Biol. 210:635-648(1989) [PubMed: 2614837] [Abstract] Cited for: STRUCTURE BY NMR. |
| [4] | "Conservative mutation Met8 --> Leu affects the folding process and structural stability of squash trypsin inhibitor CMTI-I." Zhukov I., Jaroszewski L., Bierzynski A. Protein Sci. 9:273-279(2000) [PubMed: 10716179] [Abstract] Cited for: STRUCTURE BY NMR. |
Cross-references
Sequence databases | |||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PIR | TIPU. A01313. | ||||||||||||||||||||||||||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||||||||||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||||||||||||||||||||||||||||||||
Protein family/group databases | |||||||||||||||||||||||||||||||||||||||||||||||||
| MEROPS | I07.005. | ||||||||||||||||||||||||||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||||||||||||||||||||||||||
| InterPro | IPR000737. Prot_inh_squash. [Graphical view] | ||||||||||||||||||||||||||||||||||||||||||||||||
| Pfam | PF00299. Squash. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||||||||||||||
| PRINTS | PR00293. SQUASHINHBTR. | ||||||||||||||||||||||||||||||||||||||||||||||||
| ProDom | PD003401. Prot_inh_squash. 1 hit. [Graphical view] [Entries sharing at least one domain] | ||||||||||||||||||||||||||||||||||||||||||||||||
| SMART | SM00286. PTI. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||||||||||||||
| PROSITE | PS00286. SQUASH_INHIBITOR. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||||||||||||||
| BLOCKS | Search... | ||||||||||||||||||||||||||||||||||||||||||||||||
Other Resources | |||||||||||||||||||||||||||||||||||||||||||||||||
| LinkHub | P01074. | ||||||||||||||||||||||||||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||||||||||||||||||||||||||
Entry information
| Entry name | ITR1_CUCMA | ||||||||
| Accession | Primary (citable) accession number: P01074 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |
