P01065 (IBB_VICSN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Bowman-Birk type proteinase inhibitor Alternative name(s): VAI
Organism	Vicia sativa subsp. nigra (Common vetch) (Vicia angustifolia)
Taxonomic identifier	3909 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Vicia › Vicia sativa

Protein attributes

Sequence length	72 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This inhibitor has two domains, each with separate antiprotease activity. 1 mole of inhibitor inhibits either 1 mole of trypsin or 2 moles of chymotrypsin, stoichiometrically.
Sequence similarities	Belongs to the Bowman-Birk serine protease inhibitor family.

Ontologies

Keywords
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 72	72	Bowman-Birk type proteinase inhibitor		PRO_0000105854
Sites
	Site	16 – 17	2	Reactive bond for trypsin
	Site	42 – 43	2	Reactive bond for chymotrypsin
Amino acid modifications
	Disulfide bond	8 ↔ 61		By similarity
	Disulfide bond	9 ↔ 24		By similarity
	Disulfide bond	12 ↔ 57		By similarity
	Disulfide bond	14 ↔ 22		By similarity
	Disulfide bond	31 ↔ 38		By similarity
	Disulfide bond	35 ↔ 50		By similarity
	Disulfide bond	40 ↔ 48		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P01065 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 21AADB275DE33D83	FASTA	72	8,038
	10 20 30 40 50 60 GDDVKSACCD TCLCTRSQPP TCRCVDVGER CHSACNHCVC NYSNPPQCQC FDTHKFCYKA 70 CHSSEKEEVI KN

References

[1]	"The complete amino acid sequence of Vicia angustifolia proteinase inhibitor." Shimokawa Y., Kuromizu K., Araki T., Ohata J., Abe O. Nat. Cult. 10:69-73(1983) Cited for: PROTEIN SEQUENCE. Strain: cv. Segetalis.

Cross-references

Sequence databases
PIR	TIVTOA. A01304.
3D structure databases
ProteinModelPortal	P01065.
SMR	P01065. Positions 1-67.
ModBase	Search...
Protein family/group databases
MEROPS	I12.018.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.10.69.10. 1 hit.
InterPro	IPR000877. Prot_inh_BBI. [Graphical view]
Pfam	PF00228. Bowman-Birk_leg. 2 hits. [Graphical view]
SMART	SM00269. BowB. 1 hit. [Graphical view]
SUPFAM	SSF57247. Prot_inh_BBI. 1 hit.
PROSITE	PS00281. BOWMAN_BIRK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IBB_VICSN

Accession

Primary (citable) accession number: P01065

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	March 6, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program