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Protein

Bowman-Birk type proteinase inhibitor 2

Gene

BBI

Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein inhibits elastase and trypsin simultaneously and independently.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei53 – 542Reactive bond for elastase
Sitei80 – 812Reactive bond for trypsin

GO - Molecular functioni

  • serine-type endopeptidase inhibitor activity Source: UniProtKB

GO - Biological processi

  • negative regulation of endopeptidase activity Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI12.008.

Names & Taxonomyi

Protein namesi
Recommended name:
Bowman-Birk type proteinase inhibitor 2
Alternative name(s):
Bowman-Birk type proteinase inhibitor II
Cleaved into the following 2 chains:
Gene namesi
Name:BBI
OrganismiPhaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifieri3885 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 10788Bowman-Birk type proteinase inhibitor 2 longPRO_0000375993Add
BLAST
Chaini37 – 10771Bowman-Birk type proteinase inhibitor 2 shortPRO_0000375994Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi45 ↔ 99By similarity
Disulfide bondi46 ↔ 61By similarity
Disulfide bondi49 ↔ 95By similarity
Disulfide bondi51 ↔ 59By similarity
Disulfide bondi69 ↔ 76By similarity
Disulfide bondi73 ↔ 88By similarity
Disulfide bondi78 ↔ 86By similarity

Post-translational modificationi

The N-terminus of Bowman-Birk type proteinase inhibitor 2 long is blocked.

Keywords - PTMi

Disulfide bond

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi45 – 9955Cys-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.10.69.10. 1 hit.
InterProiIPR000877. Prot_inh_BBI.
[Graphical view]
PfamiPF00228. Bowman-Birk_leg. 2 hits.
[Graphical view]
SMARTiSM00269. BowB. 1 hit.
[Graphical view]
SUPFAMiSSF57247. SSF57247. 1 hit.
PROSITEiPS00281. BOWMAN_BIRK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01060-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMVLKVCLLL VFLAGVTTAR MDLNHLIGSN HHDSSDEPSE SSEPCCDICV
60 70 80 90 100
CTASIPPICQ CTDVRLNSCH SACKSCMCTR SMPGKCRCLD TTDYCYKSCK

SSGEDDD
Length:107
Mass (Da):11,637
Last modified:May 26, 2009 - v3
Checksum:i3D7557A0A20BDC46
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti58 – 581I → Q AA sequence (PubMed:1126951).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406801 mRNA. Translation: CAL51260.1.
AM492522 Genomic DNA. Translation: CAM34247.1.
FM173201 Genomic DNA. Translation: CAQ64594.1.
PIRiA01299. TIFB2.
RefSeqiXP_007152477.1. XM_007152415.1.

Genome annotation databases

GeneIDi18632939.
KEGGipvu:PHAVU_004G133900g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406801 mRNA. Translation: CAL51260.1.
AM492522 Genomic DNA. Translation: CAM34247.1.
FM173201 Genomic DNA. Translation: CAQ64594.1.
PIRiA01299. TIFB2.
RefSeqiXP_007152477.1. XM_007152415.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI12.008.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18632939.
KEGGipvu:PHAVU_004G133900g.

Family and domain databases

Gene3Di2.10.69.10. 1 hit.
InterProiIPR000877. Prot_inh_BBI.
[Graphical view]
PfamiPF00228. Bowman-Birk_leg. 2 hits.
[Graphical view]
SMARTiSM00269. BowB. 1 hit.
[Graphical view]
SUPFAMiSSF57247. SSF57247. 1 hit.
PROSITEiPS00281. BOWMAN_BIRK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome organisation of serine proteinase inhibitors in common bean (Phaseolus vulgaris L.)."
    Galasso I., Piergiovanni A.R., Lioi L., Campion B., Bollini R., Sparvoli F.
    Mol. Breed. 23:617-624(2009)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Tissue: Cotyledon and Leaf.
  2. "Inhibitory properties and binding loop polymorphism in Bowman-Birk inhibitors from Phaseolus species."
    Galasso I., Piergiovanni A.R., Daminati G.M., Lioi L.
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Leaf.
  3. "The partial amino acid sequence of trypsin inhibitor II from garden bean, Phaseolus vulgaris, with location of the trypsin and elastase-reactive sites."
    Wilson K.A., Laskowski M. Sr.
    J. Biol. Chem. 250:4261-4267(1975) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 37-59 AND 65-107.
  4. "Isolation of three isoinhibitors of trypsin from garden bean, Phaseolus vulgaris, having either lysine or arginine at the reactive site."
    Wilson K.A., Laskowski M. Sr.
    J. Biol. Chem. 248:756-762(1973) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, FUNCTION.

Entry informationi

Entry nameiIBB2_PHAVU
AccessioniPrimary (citable) accession number: P01060
Secondary accession number(s): Q05G19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: May 26, 2009
Last modified: January 20, 2016
This is version 73 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Two forms of the inhibitor were isolated. The shorter form is probably derived by proteolytic degradation.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.