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Protein

Bowman-Birk type proteinase inhibitor 2

Gene

BBI

Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein inhibits elastase and trypsin simultaneously and independently.1 Publication

Miscellaneous

Two forms of the inhibitor were isolated. The shorter form is probably derived by proteolytic degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei53 – 54Reactive bond for elastase2
Sitei80 – 81Reactive bond for trypsin2

GO - Molecular functioni

  • serine-type endopeptidase inhibitor activity Source: UniProtKB

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI12.008.

Names & Taxonomyi

Protein namesi
Recommended name:
Bowman-Birk type proteinase inhibitor 2
Alternative name(s):
Bowman-Birk type proteinase inhibitor II
Cleaved into the following 2 chains:
Gene namesi
Name:BBI
OrganismiPhaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifieri3885 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000037599320 – 107Bowman-Birk type proteinase inhibitor 2 longAdd BLAST88
ChainiPRO_000037599437 – 107Bowman-Birk type proteinase inhibitor 2 shortAdd BLAST71

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi45 ↔ 99By similarity
Disulfide bondi46 ↔ 61By similarity
Disulfide bondi49 ↔ 95By similarity
Disulfide bondi51 ↔ 59By similarity
Disulfide bondi69 ↔ 76By similarity
Disulfide bondi73 ↔ 88By similarity
Disulfide bondi78 ↔ 86By similarity

Post-translational modificationi

The N-terminus of Bowman-Birk type proteinase inhibitor 2 long is blocked.

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

SMRiP01060.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi45 – 99Cys-richAdd BLAST55

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

CDDicd00023. BBI. 1 hit.
InterProiView protein in InterPro
IPR000877. Prot_inh_BBI.
PfamiView protein in Pfam
PF00228. Bowman-Birk_leg. 2 hits.
SMARTiView protein in SMART
SM00269. BowB. 1 hit.
SUPFAMiSSF57247. SSF57247. 1 hit.
PROSITEiView protein in PROSITE
PS00281. BOWMAN_BIRK. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01060-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMVLKVCLLL VFLAGVTTAR MDLNHLIGSN HHDSSDEPSE SSEPCCDICV
60 70 80 90 100
CTASIPPICQ CTDVRLNSCH SACKSCMCTR SMPGKCRCLD TTDYCYKSCK

SSGEDDD
Length:107
Mass (Da):11,637
Last modified:May 26, 2009 - v3
Checksum:i3D7557A0A20BDC46
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti58I → Q AA sequence (PubMed:1126951).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406801 mRNA. Translation: CAL51260.1.
AM492522 Genomic DNA. Translation: CAM34247.1.
FM173201 Genomic DNA. Translation: CAQ64594.1.
PIRiA01299. TIFB2.
RefSeqiXP_007152477.1. XM_007152415.1.

Genome annotation databases

GeneIDi18632939.
KEGGipvu:PHAVU_004G133900g.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiIBB2_PHAVU
AccessioniPrimary (citable) accession number: P01060
Secondary accession number(s): Q05G19
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: May 26, 2009
Last modified: May 10, 2017
This is version 77 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families