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Protein

Bowman-Birk type proteinase inhibitor

Gene
N/A
Organism
Phaseolus lunatus (Lima bean) (Phaseolus limensis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei26 – 272Reactive bond for trypsinBy similarity
Sitei53 – 542Reactive bond for chymotrypsinBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI12.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Bowman-Birk type proteinase inhibitor
OrganismiPhaseolus lunatus (Lima bean) (Phaseolus limensis)
Taxonomic identifieri3884 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8383Bowman-Birk type proteinase inhibitorPRO_0000105850Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi18 ↔ 72Combined sources1 Publication
Disulfide bondi19 ↔ 34Combined sources1 Publication
Disulfide bondi22 ↔ 68Combined sources1 Publication
Disulfide bondi24 ↔ 32Combined sources1 Publication
Disulfide bondi42 ↔ 49Combined sources1 Publication
Disulfide bondi46 ↔ 61Combined sources1 Publication
Disulfide bondi51 ↔ 59Combined sources1 Publication

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
83
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi20 – 245Combined sources
Beta strandi26 – 294Combined sources
Beta strandi32 – 343Combined sources
Beta strandi37 – 415Combined sources
Beta strandi47 – 515Combined sources
Beta strandi53 – 564Combined sources
Beta strandi58 – 614Combined sources
Beta strandi65 – 673Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1H34X-ray2.04A1-83[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01056.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.10.69.10. 1 hit.
InterProiIPR000877. Prot_inh_BBI.
[Graphical view]
PfamiPF00228. Bowman-Birk_leg. 2 hits.
[Graphical view]
SMARTiSM00269. BowB. 1 hit.
[Graphical view]
SUPFAMiSSF57247. SSF57247. 1 hit.
PROSITEiPS00281. BOWMAN_BIRK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P01056-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
SGHHEHSTDZ PSZSSKPCCB HCACTKSIPP QCRCTDLRLD SCHSACKSCI
60 70 80
CTLSIPAQCV CBBIBDFCYE PCKSSHSDDD NNN
Length:83
Mass (Da):9,079
Last modified:July 21, 1986 - v1
Checksum:i765DEEA0253F030D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231A → S (PubMed:12554963).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti23 – 231A → L.
Natural varianti35 – 351T → S.
Natural varianti37 – 371L → F.
Natural varianti47 – 471K → Z.
Natural varianti53 – 531L → F.
Natural varianti62 – 621B → T.
Natural varianti65 – 651B → T.

Sequence databases

PIRiA01295. TILI.

Cross-referencesi

Sequence databases

PIRiA01295. TILI.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1H34X-ray2.04A1-83[»]
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI12.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP01056.

Family and domain databases

Gene3Di2.10.69.10. 1 hit.
InterProiIPR000877. Prot_inh_BBI.
[Graphical view]
PfamiPF00228. Bowman-Birk_leg. 2 hits.
[Graphical view]
SMARTiSM00269. BowB. 1 hit.
[Graphical view]
SUPFAMiSSF57247. SSF57247. 1 hit.
PROSITEiPS00281. BOWMAN_BIRK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structure-function relationships in lima bean protease inhibitor."
    Stevens F.C., Wuerz S., Krahn J.
    (In) Fritz H., Tschesche H., Greene L.J., Truscheit E. (eds.); Proteinase inhibitors (Bayer-symp. V), pp.344-354, Springer-Verlag, Berlin (1974)
    Cited for: PROTEIN SEQUENCE.
  2. "In-house phase determination of the lima bean trypsin inhibitor: a low-resolution sulfur-SAD case."
    Debreczeni J.E., Bunkoczi G., Girmann B., Sheldrick G.M.
    Acta Crystallogr. D 59:393-395(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.04 ANGSTROMS), DISULFIDE BONDS.

Entry informationi

Entry nameiIBB_PHALU
AccessioniPrimary (citable) accession number: P01056
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: January 20, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Several variants were isolated from a commercial Lima bean preparation. Some lacked 8, 12, or 14 N-terminal residues and 2 C-terminal residues.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.