P01051 (ICIC_HIRME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Eglin C
Organism	Hirudo medicinalis (Medicinal leech)
Taxonomic identifier	6421 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Annelida › Clitellata › Hirudinida › Hirudinea › Arhynchobdellida › Hirudiniformes › Hirudinidae › Hirudo

Protein attributes

Sequence length	70 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits both elastase and cathepsin G.
Sequence similarities	Belongs to the protease inhibitor I13 (potato type I serine protease inhibitor) family. [View classification]

Ontologies

Keywords
Molecular function	Protease inhibitor Serine protease inhibitor
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	response to wounding Inferred from electronic annotation. Source: InterPro
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 70

70

Eglin C

PRO_0000217645

Sites

Site

45 – 46

2

Reactive bond

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

70

Helix Strand Turn

Details...

Helix

11 – 13

3

Helix

18 – 28

11

Beta strand

32 – 38

7

Beta strand

42 – 44

3

Beta strand

48 – 57

10

Turn

58 – 61

4

Beta strand

68 – 70

3

Sequences

	Sequence		Length	Mass (Da)
	P01051 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 7E5C5BBD39DC3C11	FASTA	70	8,091
	10 20 30 40 50 60 TEFGSELKSF PEVVGKTVDQ AREYFTLHYP QYDVYFLPEG SPVTLDLRYN RVRVFYNPGT 70 NVVNHVPHVG

References

[1]	"Structure of the elastase-cathepsin G inhibitor of the leech Hirudo medicinalis." Seemueller U., Eulitz M., Fritz H., Strobl A. Hoppe-Seyler's Z. Physiol. Chem. 361:1841-1846(1980) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	Fritz H., Seemuller U. Submitted (MAR-1982) to the PIR data bank Cited for: SEQUENCE REVISION TO 33.
[3]	"Refined 1.2 A crystal structure of the complex formed between subtilisin Carlsberg and the inhibitor eglin c. Molecular structure of eglin and its detailed interaction with subtilisin." Bode W., Papamokos E., Musil D., Seemueller U., Fritz H. EMBO J. 5:813-818(1986) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.2 ANGSTROMS) IN COMPLEX WITH SUBTILISIN.
[4]	"Sequence-specific 1H NMR assignments and secondary structure of eglin c." Hyberts S.G., Wagner G. Biochemistry 29:1465-1474(1990) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[5]	"X-ray crystal structure of the serine proteinase inhibitor eglin c at 1.95-A resolution." Hipler K., Priestle J.P., Rahuel J., Gruetter M.G. FEBS Lett. 309:139-145(1992) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
[6]	"Structure of the proteinase inhibitor eglin c with hydrolysed reactive centre at 2.0-A resolution." Betzel C., Dauter Z., Genov N., Lamzin V., Navaza J., Schnebli H.P., Visanji M., Wilson K.S. FEBS Lett. 317:185-188(1993) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

EILXCH. A94592.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ACB	X-ray	2.00	I	1-70	[»]
1CSE	X-ray	1.20	I	1-70	[»]
1EGL	NMR	-	A	1-70	[»]
1EGP	X-ray	2.00	A	1-45	[»]
			B	46-70	[»]
1MEE	X-ray	2.00	I	7-70	[»]
1SBN	X-ray	2.10	I	1-70	[»]
1SIB	X-ray	2.40	I	1-70	[»]
1TEC	X-ray	2.20	I	1-70	[»]
2SEC	X-ray	1.80	I	1-70	[»]
2TEC	X-ray	1.98	I	1-70	[»]
3TEC	X-ray	2.00	I	1-70	[»]
4B1T	X-ray	1.78	B/D	1-70	[»]
4B2A	X-ray	1.89	B/D	5-70	[»]
4B2B	X-ray	1.36	B/D	1-70	[»]
4B2C	X-ray	1.43	B/D	1-70	[»]
4H4F	X-ray	1.90	B	4-70	[»]

ProteinModelPortal

P01051.

SMR

P01051. Positions 1-70.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6069N.

MINT

MINT-1345724.

Protein family/group databases

MEROPS

I13.001.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR000864. Prot_inh_pot1.
[Graphical view]

Pfam

PF00280. potato_inhibit. 1 hit.
[Graphical view]

PRINTS

PR00292. POTATOINHBTR.

ProDom

PD002604. Prot_inh_pot1. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF54654. Prot_inh_pot1. 1 hit.

PROSITE

PS00285. POTATO_INHIBITOR. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P01051.

Entry information

Entry name

ICIC_HIRME

Accession

Primary (citable) accession number: P01051

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)