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Protein

T-kininogen 1

Gene

Map1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Kininogens are plasma glycoproteins with a number of functions: (1) as precursor of the active peptide bradykinin they effect smooth muscle contraction, induction of hypotension and increase of vascular permeability. (2) They play a role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII. (3) They are inhibitor of thiol proteases.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor, Vasoactive, Vasodilator

Keywords - Biological processi

Acute phase

Protein family/group databases

MEROPSiI25.019.

Names & Taxonomyi

Protein namesi
Recommended name:
T-kininogen 1
Alternative name(s):
Alpha-1-MAP
Major acute phase protein
T-kininogen I
Thiostatin
Cleaved into the following 3 chains:
Alternative name(s):
T-kininogen I heavy chain
Alternative name(s):
T-kininogen I light chain
Gene namesi
Name:Map1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 181 PublicationAdd BLAST18
ChainiPRO_000000669919 – 430T-kininogen 1Add BLAST412
ChainiPRO_000000670019 – 375T-kininogen 1 heavy chainAdd BLAST357
PeptideiPRO_0000006701376 – 386T-kininAdd BLAST11
ChainiPRO_0000006702387 – 430T-kininogen 1 light chainAdd BLAST44

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19Pyrrolidone carboxylic acid1 Publication1
Disulfide bondi28 ↔ 404Interchain (between heavy and light chains)PROSITE-ProRule annotation
Glycosylationi82N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi83 ↔ 94PROSITE-ProRule annotation
Disulfide bondi107 ↔ 125PROSITE-ProRule annotation
Glycosylationi126N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi141 ↔ 144PROSITE-ProRule annotation
Glycosylationi168N-linked (GlcNAc...)Sequence analysis1
Glycosylationi204N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi205 ↔ 217PROSITE-ProRule annotation
Disulfide bondi228 ↔ 247PROSITE-ProRule annotation
Disulfide bondi263 ↔ 266PROSITE-ProRule annotation
Glycosylationi326N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi327 ↔ 339PROSITE-ProRule annotation
Disulfide bondi350 ↔ 369PROSITE-ProRule annotation

Post-translational modificationi

As T-kinin is preceded by a Met instead of an Arg or Lys, it is not released from its precursor by either tissue or plasma kallikrein.

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP01048.
PRIDEiP01048.

PTM databases

UniCarbKBiP01048.

Miscellaneous databases

PMAP-CutDBP01048.

Expressioni

Tissue specificityi

Plasma.

Inductioni

In response to an inflammatory stimulant. T-kininogen II synthesis is induced and the plasma concentration of T-kininogen I is raised.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000055278.

Structurei

3D structure databases

ProteinModelPortaliP01048.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 131Cystatin kininogen-type 1PROSITE-ProRule annotationAdd BLAST104
Domaini150 – 253Cystatin kininogen-type 2PROSITE-ProRule annotationAdd BLAST104
Domaini272 – 375Cystatin kininogen-type 3PROSITE-ProRule annotationAdd BLAST104

Sequence similaritiesi

Contains 3 cystatin kininogen-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IKIQ. Eukaryota.
ENOG4111ZQ8. LUCA.
HOGENOMiHOG000113239.
HOVERGENiHBG006224.
InParanoidiP01048.
PhylomeDBiP01048.

Family and domain databases

CDDicd00042. CY. 3 hits.
InterProiIPR000010. Cystatin_dom.
IPR027358. Kininogen-type_cystatin_dom.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PfamiPF00031. Cystatin. 3 hits.
[Graphical view]
SMARTiSM00043. CY. 3 hits.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 2 hits.
PS51647. CYSTATIN_KININOGEN. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01048-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLITILLLC SRLLPSLAQE EGAQELNCND ETVFQAVDTA LKKYNAELES
60 70 80 90 100
GNQFVLYRVT EGTKKDGAET LYSFKYQIKE GNCSVQSGLT WQDCDFKDAE
110 120 130 140 150
EAATGECTTT LGKKENKFSV ATQICNITPG KGPKKTEEDL CVGCFQPIPM
160 170 180 190 200
DSSDLKPVLK HAVEHFNNNT KHTHLFALRE VKSAHSQVVA GMNYKIIYSI
210 220 230 240 250
VQTNCSKEDF PSLREDCVPL PYGDHGECTG HTHVDIHNTI AGFSQSCDLY
260 270 280 290 300
PGDDLFELLP KNCRGCPREI PVDSPELKEA LGHSIAQLNA QHNHIFYFKI
310 320 330 340 350
DTVKKATSQV VAGVIYVIEF IARETNCSKQ SKTELTADCE TKHLGQSLNC
360 370 380 390 400
NANVYMRPWE NKVVPTVRCQ ALDMMISRPP GFSPFRLVRV QETKEGTTRL
410 420 430
LNSCEYKGRL SKARAGPAPD HQAEASTVTP
Length:430
Mass (Da):47,775
Last modified:February 1, 2005 - v2
Checksum:iFEBBF28FA44793C3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7L → V in CAA26162 (PubMed:2578992).Curated1
Sequence conflicti19Q → E AA sequence (PubMed:3121623).Curated1
Sequence conflicti61E → K in AAA41489 (PubMed:2413018).Curated1
Sequence conflicti114K → R no nucleotide entry (PubMed:2806908).Curated1
Sequence conflicti166F → S in AAA41489 (PubMed:2413018).Curated1
Sequence conflicti179 – 181REV → TKI no nucleotide entry (PubMed:2806908).Curated3
Sequence conflicti212S → F no nucleotide entry (PubMed:2806908).Curated1
Sequence conflicti214R → H in CAA26162 (PubMed:2578992).Curated1
Sequence conflicti257E → S no nucleotide entry (PubMed:2806908).Curated1
Sequence conflicti287Q → R in CAA26162 (PubMed:2578992).Curated1
Sequence conflicti389R → Q no nucleotide entry (PubMed:2806908).Curated1
Sequence conflicti414R → G no nucleotide entry (PubMed:2806908).Curated1
Sequence conflicti414R → G in CAA26162 (PubMed:2578992).Curated1
Sequence conflicti420D → E in CAA26162 (PubMed:2578992).Curated1
Sequence conflicti421H → R in CAA26162 (PubMed:2578992).Curated1
Sequence conflicti421H → R no nucleotide entry (PubMed:2108948).Curated1
Sequence conflicti430P → S in AAA41489 (PubMed:2413018).Curated1
Sequence conflicti430P → S AA sequence (PubMed:3121623).Curated1
Sequence conflicti430P → S (PubMed:7574705).Curated1
Sequence conflicti430P → S (PubMed:3029068).Curated1
Sequence conflicti430P → S AA sequence (PubMed:3335530).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11883 mRNA. Translation: AAA41489.1.
M16454 Genomic DNA. Translation: AAA41568.1.
M14356 Genomic DNA. Translation: AAA41492.1.
X02299 mRNA. Translation: CAA26162.1. Sequence problems.
PIRiA01285. KGRTM.
A01286. KGRTT1.
A23897.
A27115.
UniGeneiRn.128333.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11883 mRNA. Translation: AAA41489.1.
M16454 Genomic DNA. Translation: AAA41568.1.
M14356 Genomic DNA. Translation: AAA41492.1.
X02299 mRNA. Translation: CAA26162.1. Sequence problems.
PIRiA01285. KGRTM.
A01286. KGRTT1.
A23897.
A27115.
UniGeneiRn.128333.

3D structure databases

ProteinModelPortaliP01048.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000055278.

Protein family/group databases

MEROPSiI25.019.

PTM databases

UniCarbKBiP01048.

Proteomic databases

PaxDbiP01048.
PRIDEiP01048.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410IKIQ. Eukaryota.
ENOG4111ZQ8. LUCA.
HOGENOMiHOG000113239.
HOVERGENiHBG006224.
InParanoidiP01048.
PhylomeDBiP01048.

Miscellaneous databases

PMAP-CutDBP01048.
PROiP01048.

Family and domain databases

CDDicd00042. CY. 3 hits.
InterProiIPR000010. Cystatin_dom.
IPR027358. Kininogen-type_cystatin_dom.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PfamiPF00031. Cystatin. 3 hits.
[Graphical view]
SMARTiSM00043. CY. 3 hits.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 2 hits.
PS51647. CYSTATIN_KININOGEN. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKNT1_RAT
AccessioniPrimary (citable) accession number: P01048
Secondary accession number(s): P04081, Q6LDW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: February 1, 2005
Last modified: November 30, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Rats express four types of kininogens: the classical HMW and LMW kininogens produced by alternative splicing of the same gene, and two additional LMW-like kininogens: T-I and T-II.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.