Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

T-kininogen 1

Gene

Map1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Kininogens are plasma glycoproteins with a number of functions: (1) as precursor of the active peptide bradykinin they effect smooth muscle contraction, induction of hypotension and increase of vascular permeability. (2) They play a role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII. (3) They are inhibitor of thiol proteases.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor, Vasoactive, Vasodilator

Keywords - Biological processi

Acute phase

Protein family/group databases

MEROPSiI25.019.

Names & Taxonomyi

Protein namesi
Recommended name:
T-kininogen 1
Alternative name(s):
Alpha-1-MAP
Major acute phase protein
T-kininogen I
Thiostatin
Cleaved into the following 3 chains:
Alternative name(s):
T-kininogen I heavy chain
Alternative name(s):
T-kininogen I light chain
Gene namesi
Name:Map1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 18181 PublicationAdd
BLAST
Chaini19 – 430412T-kininogen 1PRO_0000006699Add
BLAST
Chaini19 – 375357T-kininogen 1 heavy chainPRO_0000006700Add
BLAST
Peptidei376 – 38611T-kininPRO_0000006701Add
BLAST
Chaini387 – 43044T-kininogen 1 light chainPRO_0000006702Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Pyrrolidone carboxylic acid1 Publication
Disulfide bondi28 ↔ 404Interchain (between heavy and light chains)PROSITE-ProRule annotation
Glycosylationi82 – 821N-linked (GlcNAc...)Sequence analysis
Disulfide bondi83 ↔ 94PROSITE-ProRule annotation
Disulfide bondi107 ↔ 125PROSITE-ProRule annotation
Glycosylationi126 – 1261N-linked (GlcNAc...)Sequence analysis
Disulfide bondi141 ↔ 144PROSITE-ProRule annotation
Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence analysis
Glycosylationi204 – 2041N-linked (GlcNAc...)Sequence analysis
Disulfide bondi205 ↔ 217PROSITE-ProRule annotation
Disulfide bondi228 ↔ 247PROSITE-ProRule annotation
Disulfide bondi263 ↔ 266PROSITE-ProRule annotation
Glycosylationi326 – 3261N-linked (GlcNAc...)Sequence analysis
Disulfide bondi327 ↔ 339PROSITE-ProRule annotation
Disulfide bondi350 ↔ 369PROSITE-ProRule annotation

Post-translational modificationi

As T-kinin is preceded by a Met instead of an Arg or Lys, it is not released from its precursor by either tissue or plasma kallikrein.

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP01048.
PRIDEiP01048.

PTM databases

UniCarbKBiP01048.

Miscellaneous databases

PMAP-CutDBP01048.

Expressioni

Tissue specificityi

Plasma.

Inductioni

In response to an inflammatory stimulant. T-kininogen II synthesis is induced and the plasma concentration of T-kininogen I is raised.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000055278.

Structurei

3D structure databases

ProteinModelPortaliP01048.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 131104Cystatin kininogen-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini150 – 253104Cystatin kininogen-type 2PROSITE-ProRule annotationAdd
BLAST
Domaini272 – 375104Cystatin kininogen-type 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 cystatin kininogen-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IKIQ. Eukaryota.
ENOG4111ZQ8. LUCA.
HOGENOMiHOG000113239.
HOVERGENiHBG006224.
InParanoidiP01048.
PhylomeDBiP01048.

Family and domain databases

InterProiIPR000010. Cystatin_dom.
IPR027358. Kininogen-type_cystatin_dom.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PfamiPF00031. Cystatin. 3 hits.
[Graphical view]
SMARTiSM00043. CY. 3 hits.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 2 hits.
PS51647. CYSTATIN_KININOGEN. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01048-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLITILLLC SRLLPSLAQE EGAQELNCND ETVFQAVDTA LKKYNAELES
60 70 80 90 100
GNQFVLYRVT EGTKKDGAET LYSFKYQIKE GNCSVQSGLT WQDCDFKDAE
110 120 130 140 150
EAATGECTTT LGKKENKFSV ATQICNITPG KGPKKTEEDL CVGCFQPIPM
160 170 180 190 200
DSSDLKPVLK HAVEHFNNNT KHTHLFALRE VKSAHSQVVA GMNYKIIYSI
210 220 230 240 250
VQTNCSKEDF PSLREDCVPL PYGDHGECTG HTHVDIHNTI AGFSQSCDLY
260 270 280 290 300
PGDDLFELLP KNCRGCPREI PVDSPELKEA LGHSIAQLNA QHNHIFYFKI
310 320 330 340 350
DTVKKATSQV VAGVIYVIEF IARETNCSKQ SKTELTADCE TKHLGQSLNC
360 370 380 390 400
NANVYMRPWE NKVVPTVRCQ ALDMMISRPP GFSPFRLVRV QETKEGTTRL
410 420 430
LNSCEYKGRL SKARAGPAPD HQAEASTVTP
Length:430
Mass (Da):47,775
Last modified:February 1, 2005 - v2
Checksum:iFEBBF28FA44793C3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71L → V in CAA26162 (PubMed:2578992).Curated
Sequence conflicti19 – 191Q → E AA sequence (PubMed:3121623).Curated
Sequence conflicti61 – 611E → K in AAA41489 (PubMed:2413018).Curated
Sequence conflicti114 – 1141K → R no nucleotide entry (PubMed:2806908).Curated
Sequence conflicti166 – 1661F → S in AAA41489 (PubMed:2413018).Curated
Sequence conflicti179 – 1813REV → TKI no nucleotide entry (PubMed:2806908).Curated
Sequence conflicti212 – 2121S → F no nucleotide entry (PubMed:2806908).Curated
Sequence conflicti214 – 2141R → H in CAA26162 (PubMed:2578992).Curated
Sequence conflicti257 – 2571E → S no nucleotide entry (PubMed:2806908).Curated
Sequence conflicti287 – 2871Q → R in CAA26162 (PubMed:2578992).Curated
Sequence conflicti389 – 3891R → Q no nucleotide entry (PubMed:2806908).Curated
Sequence conflicti414 – 4141R → G no nucleotide entry (PubMed:2806908).Curated
Sequence conflicti414 – 4141R → G in CAA26162 (PubMed:2578992).Curated
Sequence conflicti420 – 4201D → E in CAA26162 (PubMed:2578992).Curated
Sequence conflicti421 – 4211H → R in CAA26162 (PubMed:2578992).Curated
Sequence conflicti421 – 4211H → R no nucleotide entry (PubMed:2108948).Curated
Sequence conflicti430 – 4301P → S in AAA41489 (PubMed:2413018).Curated
Sequence conflicti430 – 4301P → S AA sequence (PubMed:3121623).Curated
Sequence conflicti430 – 4301P → S (PubMed:7574705).Curated
Sequence conflicti430 – 4301P → S (PubMed:3029068).Curated
Sequence conflicti430 – 4301P → S AA sequence (PubMed:3335530).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11883 mRNA. Translation: AAA41489.1.
M16454 Genomic DNA. Translation: AAA41568.1.
M14356 Genomic DNA. Translation: AAA41492.1.
X02299 mRNA. Translation: CAA26162.1. Sequence problems.
PIRiA01285. KGRTM.
A01286. KGRTT1.
A23897.
A27115.
UniGeneiRn.128333.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11883 mRNA. Translation: AAA41489.1.
M16454 Genomic DNA. Translation: AAA41568.1.
M14356 Genomic DNA. Translation: AAA41492.1.
X02299 mRNA. Translation: CAA26162.1. Sequence problems.
PIRiA01285. KGRTM.
A01286. KGRTT1.
A23897.
A27115.
UniGeneiRn.128333.

3D structure databases

ProteinModelPortaliP01048.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000055278.

Protein family/group databases

MEROPSiI25.019.

PTM databases

UniCarbKBiP01048.

Proteomic databases

PaxDbiP01048.
PRIDEiP01048.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410IKIQ. Eukaryota.
ENOG4111ZQ8. LUCA.
HOGENOMiHOG000113239.
HOVERGENiHBG006224.
InParanoidiP01048.
PhylomeDBiP01048.

Miscellaneous databases

PMAP-CutDBP01048.
PROiP01048.

Family and domain databases

InterProiIPR000010. Cystatin_dom.
IPR027358. Kininogen-type_cystatin_dom.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PfamiPF00031. Cystatin. 3 hits.
[Graphical view]
SMARTiSM00043. CY. 3 hits.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 2 hits.
PS51647. CYSTATIN_KININOGEN. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Primary structures of the mRNAs encoding the rat precursors for bradykinin and T-kinin. Structural relationship of kininogens with major acute phase protein and alpha 1-cysteine proteinase inhibitor."
    Furuto-Kato S., Matsumoto A., Kitamura N., Nakanishi S.
    J. Biol. Chem. 260:12054-12059(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Primary structure of a gene encoding rat T-kininogen."
    Anderson K.P., Croyle M.L., Lingrel J.B.
    Gene 81:119-128(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
  3. "Structure and expression of the genes for major acute phase alpha 1-protein (thiostatin) and kininogen in the rat."
    Fung W.-P., Schreiber G.
    J. Biol. Chem. 262:9298-9308(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-65.
  4. "Differing utilization of homologous transcription initiation sites of rat K and T kininogen genes under inflammation condition."
    Kageyama R., Kitamura N., Ohkubo H., Nakanishi S.
    J. Biol. Chem. 262:2345-2351(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-48.
    Strain: Wistar.
    Tissue: Liver.
  5. "Major acute phase alpha 1-protein of the rat is homologous to bovine kininogen and contains the sequence for bradykinin: its synthesis is regulated at the mRNA level."
    Cole T., Inglis A.S., Roxburgh C.M., Howlett G.J., Schreiber G.
    FEBS Lett. 182:57-61(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-430.
  6. "Purification and characterization of rat T-kininogens isolated from plasma of adjuvant-treated rats. Identification of three kinds of T-kininogens."
    Enjyoji K., Kato H., Hayashi I., Oh-ishi S., Iwanaga S.
    J. Biol. Chem. 263:973-979(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 19-48 AND 376-430, PYROGLUTAMATE FORMATION AT GLN-19.
  7. "Identification of a protein increasing in serum of Nagase analbuminemic rats bearing intestinal tumors as an isotype of T-kininogen."
    Kanda S., Sugiyama K., Takahashi M., Shumiya S., Tomino S., Nagase S.
    Jpn. J. Cancer Res. 81:63-68(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 330-430.
  8. "Identification of several isoforms of T-kininogen expressed in the liver of aging rats."
    Sierra F., Walter R., Vautravers P., Guigoz Y.
    Arch. Biochem. Biophys. 322:333-338(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 340-430.
  9. "Differing expression patterns and evolution of the rat kininogen gene family."
    Kitagawa H., Kitamura N., Hayashida H., Miyata T., Nakanishi S.
    J. Biol. Chem. 262:2190-2198(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 375-430.
  10. "Purification and characterization of two kinds of low molecular weight kininogens from rat (non-inflamed) plasma. One resistant and the second sensitive to rat glandular kallikreins."
    Enjyoji K., Kato H., Hayashi I., Oh-ishi S., Iwanaga S.
    J. Biol. Chem. 263:965-972(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 376-430.

Entry informationi

Entry nameiKNT1_RAT
AccessioniPrimary (citable) accession number: P01048
Secondary accession number(s): P04081, Q6LDW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: February 1, 2005
Last modified: May 11, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Rats express four types of kininogens: the classical HMW and LMW kininogens produced by alternative splicing of the same gene, and two additional LMW-like kininogens: T-I and T-II.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.