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P01040

- CYTA_HUMAN

UniProt

P01040 - CYTA_HUMAN

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Protein

Cystatin-A

Gene
CSTA, STF1, STFA
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei4 – 41Reactive site

GO - Molecular functioni

  1. cysteine-type endopeptidase inhibitor activity Source: BHF-UCL
  2. protease binding Source: BHF-UCL
  3. protein binding, bridging Source: UniProtKB
  4. structural molecule activity Source: UniProtKB

GO - Biological processi

  1. keratinocyte differentiation Source: UniProtKB
  2. negative regulation of endopeptidase activity Source: GOC
  3. negative regulation of peptidase activity Source: BHF-UCL
  4. negative regulation of proteolysis Source: UniProt
  5. peptide cross-linking Source: UniProtKB
  6. single organismal cell-cell adhesion Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor

Keywords - Biological processi

Cell adhesion

Protein family/group databases

MEROPSiI25.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystatin-A
Alternative name(s):
Cystatin-AS
Stefin-A
Cleaved into the following chain:
Gene namesi
Name:CSTA
Synonyms:STF1, STFA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:2481. CSTA.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cornified envelope Source: UniProtKB
  2. cytoplasm Source: UniProtKB
  3. extracellular space Source: UniProt
  4. extracellular vesicular exosome Source: UniProt
  5. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Involvement in diseasei

Ichthyosis, exfoliative, autosomal recessive, ichthyosis bullosa of Siemens-like (AREI) [MIM:607936]: A form of congenital exfoliative ichthyosis, sharing some features with ichthyosis bullosa of Siemens and annular epidermolytic ichthyosis. AREI presents shortly after birth as dry, scaly skin over most of the body with coarse peeling of non-erythematous skin on the palms and soles, which is exacerbated by excessive moisture and minor trauma. Electron microscopy analysis of skin biopsies, reveals mostly normal-appearing upper layers of the epidermis, but prominent intercellular edema of the basal and suprabasal cell layers with aggregates of tonofilaments in the basal keratinocytes.
Note: The disease is caused by mutations affecting the gene represented in this entry.1 Publication

Keywords - Diseasei

Ichthyosis

Organism-specific databases

MIMi607936. phenotype.
Orphaneti289586. Exfoliative ichthyosis.
PharmGKBiPA26983.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9898Cystatin-APRO_0000423202Add
BLAST
Initiator methioninei1 – 11Removed; alternate1 Publication
Chaini2 – 9897Cystatin-A, N-terminally processedPRO_0000207128Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP01040.
PaxDbiP01040.
PeptideAtlasiP01040.
PRIDEiP01040.

PTM databases

PhosphoSiteiP01040.

Expressioni

Tissue specificityi

Expressed in the skin throughout the epidermis.1 Publication

Gene expression databases

ArrayExpressiP01040.
BgeeiP01040.
CleanExiHS_CSTA.
GenevestigatoriP01040.

Organism-specific databases

HPAiCAB000469.
CAB047315.
HPA001031.

Interactioni

Protein-protein interaction databases

BioGridi107857. 18 interactions.
IntActiP01040. 9 interactions.
MINTiMINT-1428155.
STRINGi9606.ENSP00000264474.

Structurei

Secondary structure

1
98
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 126
Helixi14 – 3118
Beta strandi34 – 363
Beta strandi39 – 5820
Helixi60 – 623
Beta strandi64 – 718
Helixi74 – 763
Helixi77 – 793
Beta strandi80 – 8910
Beta strandi91 – 933

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CYUNMR-A1-98[»]
1CYVNMR-A1-98[»]
1DVCNMR-A1-98[»]
1DVDNMR-A1-98[»]
1GD3NMR-A1-98[»]
1GD4NMR-A1-98[»]
1N9JNMR-A/B1-98[»]
1NB3X-ray2.80I/J/K/L1-98[»]
1NB5X-ray2.40I/J/K/L1-98[»]
3K9MX-ray2.61C/D1-98[»]
3KFQX-ray1.99C/D1-98[»]
3KSEX-ray1.71D/E/F1-98[»]
ProteinModelPortaliP01040.
SMRiP01040. Positions 1-98.

Miscellaneous databases

EvolutionaryTraceiP01040.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 505Secondary area of contact

Sequence similaritiesi

Belongs to the cystatin family.

Phylogenomic databases

eggNOGiNOG119299.
HOGENOMiHOG000294175.
HOVERGENiHBG002292.
InParanoidiP01040.
KOiK13907.
OMAiIEYKTQV.
OrthoDBiEOG7FR7JX.
PhylomeDBiP01040.
TreeFamiTF333174.

Family and domain databases

InterProiIPR000010. Prot_inh_cystat.
IPR018073. Prot_inh_cystat_CS.
IPR001713. Prot_inh_stefinA.
[Graphical view]
PfamiPF00031. Cystatin. 1 hit.
[Graphical view]
PRINTSiPR00295. STEFINA.
SMARTiSM00043. CY. 1 hit.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01040-1 [UniParc]FASTAAdd to Basket

« Hide

MIPGGLSEAK PATPEIQEIV DKVKPQLEEK TNETYGKLEA VQYKTQVVAG   50
TNYYIKVRAG DNKYMHLKVF KSLPGQNEDL VLTGYQVDKN KDDELTGF 98
Length:98
Mass (Da):11,006
Last modified:July 21, 1986 - v1
Checksum:i2F5F0D61C91305EE
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti96 – 961T → M.
Corresponds to variant rs34173813 [ dbSNP | Ensembl ].
VAR_048851

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X05978 mRNA. Translation: CAA29398.1.
D88422 Genomic DNA. Translation: BAA13609.1.
AB007774 Genomic DNA. Translation: BAA87858.1.
AK291308 mRNA. Translation: BAF83997.1.
CR456914 mRNA. Translation: CAG33195.1.
CH471052 Genomic DNA. Translation: EAW79489.1.
BC010379 mRNA. Translation: AAH10379.1.
CCDSiCCDS3011.1.
PIRiA29139. UDHUS.
RefSeqiNP_005204.1. NM_005213.3.
UniGeneiHs.518198.

Genome annotation databases

EnsembliENST00000264474; ENSP00000264474; ENSG00000121552.
GeneIDi1475.
KEGGihsa:1475.
UCSCiuc003eex.3. human.

Polymorphism databases

DMDMi118177.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X05978 mRNA. Translation: CAA29398.1 .
D88422 Genomic DNA. Translation: BAA13609.1 .
AB007774 Genomic DNA. Translation: BAA87858.1 .
AK291308 mRNA. Translation: BAF83997.1 .
CR456914 mRNA. Translation: CAG33195.1 .
CH471052 Genomic DNA. Translation: EAW79489.1 .
BC010379 mRNA. Translation: AAH10379.1 .
CCDSi CCDS3011.1.
PIRi A29139. UDHUS.
RefSeqi NP_005204.1. NM_005213.3.
UniGenei Hs.518198.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1CYU NMR - A 1-98 [» ]
1CYV NMR - A 1-98 [» ]
1DVC NMR - A 1-98 [» ]
1DVD NMR - A 1-98 [» ]
1GD3 NMR - A 1-98 [» ]
1GD4 NMR - A 1-98 [» ]
1N9J NMR - A/B 1-98 [» ]
1NB3 X-ray 2.80 I/J/K/L 1-98 [» ]
1NB5 X-ray 2.40 I/J/K/L 1-98 [» ]
3K9M X-ray 2.61 C/D 1-98 [» ]
3KFQ X-ray 1.99 C/D 1-98 [» ]
3KSE X-ray 1.71 D/E/F 1-98 [» ]
ProteinModelPortali P01040.
SMRi P01040. Positions 1-98.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 107857. 18 interactions.
IntActi P01040. 9 interactions.
MINTi MINT-1428155.
STRINGi 9606.ENSP00000264474.

Protein family/group databases

MEROPSi I25.001.

PTM databases

PhosphoSitei P01040.

Polymorphism databases

DMDMi 118177.

Proteomic databases

MaxQBi P01040.
PaxDbi P01040.
PeptideAtlasi P01040.
PRIDEi P01040.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000264474 ; ENSP00000264474 ; ENSG00000121552 .
GeneIDi 1475.
KEGGi hsa:1475.
UCSCi uc003eex.3. human.

Organism-specific databases

CTDi 1475.
GeneCardsi GC03P122044.
HGNCi HGNC:2481. CSTA.
HPAi CAB000469.
CAB047315.
HPA001031.
MIMi 184600. gene.
607936. phenotype.
neXtProti NX_P01040.
Orphaneti 289586. Exfoliative ichthyosis.
PharmGKBi PA26983.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG119299.
HOGENOMi HOG000294175.
HOVERGENi HBG002292.
InParanoidi P01040.
KOi K13907.
OMAi IEYKTQV.
OrthoDBi EOG7FR7JX.
PhylomeDBi P01040.
TreeFami TF333174.

Miscellaneous databases

EvolutionaryTracei P01040.
GeneWikii Cystatin_A.
GenomeRNAii 1475.
NextBioi 6057.
PROi P01040.
SOURCEi Search...

Gene expression databases

ArrayExpressi P01040.
Bgeei P01040.
CleanExi HS_CSTA.
Genevestigatori P01040.

Family and domain databases

InterProi IPR000010. Prot_inh_cystat.
IPR018073. Prot_inh_cystat_CS.
IPR001713. Prot_inh_stefinA.
[Graphical view ]
Pfami PF00031. Cystatin. 1 hit.
[Graphical view ]
PRINTSi PR00295. STEFINA.
SMARTi SM00043. CY. 1 hit.
[Graphical view ]
PROSITEi PS00287. CYSTATIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Protein inhibitors of cysteine proteinases. II. Primary structure of stefin, a cytosolic protein inhibitor of cysteine proteinases from human polymorphonuclear granulocytes."
    Machleidt W., Borchart U., Fritz H., Brzin J., Ritonja A., Turk V.
    Hoppe-Seyler's Z. Physiol. Chem. 364:1481-1486(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  2. "Comparative studies on the primary structure of human cystatin as from epidermis, liver, spleen, and leukocytes."
    Takeda A., Kaji H., Nakaya K., Nakmura Y., Samejima T.
    J. Biochem. 105:986-991(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  3. "Effects of UV, 4-NQO and TPA on gene expression in cultured human epidermal keratinocytes."
    Kartasova T., Cornelissen B.J.C., Belt P., van de Putte P.
    Nucleic Acids Res. 15:5945-5962(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Genomic structure of human cystatin A."
    Yamazaki M., Ishidoh K., Eiki K., Ogawa H.
    DNA Seq. 8:71-76(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Structure and transcriptional regulation of the human cystatin A gene. The 12-o-tetradecanoylphorbol-13-acetate (tpa) responsive element-2 site (-272 to -278) on cystatin a gene is critical for tpa-dependent regulation."
    Takahashi H., Asano K., Kinouchi M., Ishida-Yamamoto A., Wuepper K.D., Iizuka H.
    J. Biol. Chem. 273:17375-17380(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Tongue.
  7. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone marrow.
  10. Bienvenut W.V., Gao M., Leug H.
    Submitted (JUN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-22; 31-56 AND 72-98, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Prostatic carcinoma.
  11. "Molecular cloning, occurrence, and expression of a novel partially secreted protein 'psoriasin' that is highly up-regulated in psoriatic skin."
    Madsen P., Rasmussen H.H., Leffers H., Honore B., Dejgaard K., Olsen E., Kiil J., Walbum E., Andersen A.H., Basse B., Lauridsen J.B., Ratz G.P., Celis A., Vandekerckhove J., Celis J.E.
    J. Invest. Dermatol. 97:701-712(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 72-85.
    Tissue: Keratinocyte.
  12. "Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes."
    Rasmussen H.H., van Damme J., Puype M., Gesser B., Celis J.E., Vandekerckhove J.
    Electrophoresis 13:960-969(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 72-85.
    Tissue: Keratinocyte.
  13. "Mutations in CSTA, encoding Cystatin A, underlie exfoliative ichthyosis and reveal a role for this protease inhibitor in cell-cell adhesion."
    Blaydon D.C., Nitoiu D., Eckl K.M., Cabral R.M., Bland P., Hausser I., van Heel D.A., Rajpopat S., Fischer J., Oji V., Zvulunov A., Traupe H., Hennies H.C., Kelsell D.P.
    Am. J. Hum. Genet. 89:564-571(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INVOLVEMENT IN AREI.
  14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. Cited for: STRUCTURE BY NMR.
  16. "Solution structure of a human cystatin A variant, cystatin A2-98 M65L, by NMR spectroscopy. A possible role of the interactions between the N- and C-termini to maintain the inhibitory active form of cystatin A."
    Tate S., Ushioda T., Utsunomiya-Tate N., Shibuya K., Ohyama Y., Nakano Y., Kaji H., Inagaki F., Samejima T., Kainosho M.
    Biochemistry 34:14637-14648(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiCYTA_HUMAN
AccessioniPrimary (citable) accession number: P01040
Secondary accession number(s): Q6IB90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 3, 2014
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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