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Protein

Cystatin

Gene
N/A
Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein binds tightly to and inhibits a variety of thiol proteases including ficin, papain, and cathepsins B, C, H, and L. Although isolated from egg white, it is also present in serum.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei32 – 321Reactive site

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor

Protein family/group databases

MEROPSiI25.011.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystatin
Alternative name(s):
Egg-white cystatin
Ovocystatin
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23232 PublicationsAdd
BLAST
Chaini24 – 139116CystatinPRO_0000006663Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi94 ↔ 1041 Publication
Modified residuei103 – 1031Phosphoserine1 Publication
Disulfide bondi118 ↔ 1381 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP01038.
PRIDEiP01038.

PTM databases

iPTMnetiP01038.

Expressioni

Gene expression databases

ExpressionAtlasiP01038. baseline.

Interactioni

Protein-protein interaction databases

DIPiDIP-61237N.
STRINGi9031.ENSGALP00000014093.

Structurei

Secondary structure

1
139
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi42 – 5110Combined sources
Helixi53 – 564Combined sources
Beta strandi62 – 9534Combined sources
Beta strandi96 – 994Combined sources
Helixi101 – 1088Combined sources
Beta strandi115 – 12511Combined sources
Turni126 – 1294Combined sources
Beta strandi130 – 1389Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A67NMR-A32-139[»]
1A90NMR-A32-139[»]
1CEWX-ray2.00I32-139[»]
1YVBX-ray2.70I29-139[»]
ProteinModelPortaliP01038.
SMRiP01038. Positions 29-139.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01038.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi76 – 805Secondary area of contact

Sequence similaritiesi

Belongs to the cystatin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IZZH. Eukaryota.
ENOG4112CFJ. LUCA.
HOGENOMiHOG000231754.
HOVERGENiHBG009556.
InParanoidiP01038.
KOiK13899.
PhylomeDBiP01038.

Family and domain databases

InterProiIPR027214. Cystatin.
IPR000010. Cystatin_dom.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PANTHERiPTHR11413. PTHR11413. 1 hit.
PfamiPF00031. Cystatin. 1 hit.
[Graphical view]
SMARTiSM00043. CY. 1 hit.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01038-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGARGCVVL LAAALMLVGA VLGSEDRSRL LGAPVPVDEN DEGLQRALQF
60 70 80 90 100
AMAEYNRASN DKYSSRVVRV ISAKRQLVSG IKYILQVEIG RTTCPKSSGD
110 120 130
LQSCEFHDEP EMAKYTTCTF VVYSIPWLNQ IKLLESKCQ
Length:139
Mass (Da):15,287
Last modified:October 1, 1989 - v2
Checksum:iD92D1131C4D37891
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05077 mRNA. Translation: AAA48744.1.
PIRiA34456. UDCH.
RefSeqiNP_990831.2. NM_205500.2.
UniGeneiGga.5058.

Genome annotation databases

GeneIDi396497.
KEGGigga:396497.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05077 mRNA. Translation: AAA48744.1.
PIRiA34456. UDCH.
RefSeqiNP_990831.2. NM_205500.2.
UniGeneiGga.5058.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A67NMR-A32-139[»]
1A90NMR-A32-139[»]
1CEWX-ray2.00I32-139[»]
1YVBX-ray2.70I29-139[»]
ProteinModelPortaliP01038.
SMRiP01038. Positions 29-139.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61237N.
STRINGi9031.ENSGALP00000014093.

Protein family/group databases

MEROPSiI25.011.

PTM databases

iPTMnetiP01038.

Proteomic databases

PaxDbiP01038.
PRIDEiP01038.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396497.
KEGGigga:396497.

Organism-specific databases

CTDi1471.

Phylogenomic databases

eggNOGiENOG410IZZH. Eukaryota.
ENOG4112CFJ. LUCA.
HOGENOMiHOG000231754.
HOVERGENiHBG009556.
InParanoidiP01038.
KOiK13899.
PhylomeDBiP01038.

Miscellaneous databases

EvolutionaryTraceiP01038.
PROiP01038.

Gene expression databases

ExpressionAtlasiP01038. baseline.

Family and domain databases

InterProiIPR027214. Cystatin.
IPR000010. Cystatin_dom.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PANTHERiPTHR11413. PTHR11413. 1 hit.
PfamiPF00031. Cystatin. 1 hit.
[Graphical view]
SMARTiSM00043. CY. 1 hit.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Chicken egg white cystatin. Molecular cloning, nucleotide sequence, and tissue distribution."
    Colella R., Sakaguchi Y., Nagase H., Bird J.W.C.
    J. Biol. Chem. 264:17164-17169(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cystatin. Amino acid sequence and possible secondary structure."
    Schwabe C., Anastasi A., Crow H., McDonald J.K., Barrett A.J.
    Biochem. J. 217:813-817(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-139.
  3. "Protein inhibitors of cysteine proteinases. III. Amino-acid sequence of cystatin from chicken egg white."
    Turk V., Brzin J., Longer M., Ritonja A., Eropkin M., Borchart U., Machleidt W.
    Hoppe-Seyler's Z. Physiol. Chem. 364:1487-1496(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-139.
  4. "Cystatin, a protein inhibitor of cysteine proteinases. Improved purification from egg white, characterization, and detection in chicken serum."
    Anastasi A., Brown M.A., Kembhavi A.A., Nicklin M.J.H., Sayers C.A., Sunter D.C., Barrett A.J.
    Biochem. J. 211:129-138(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF PROTEIN.
  5. "The disulphide bridges of human cystatin C (gamma-trace) and chicken cystatin."
    Grubb A., Loefberg H., Barrett A.J.
    FEBS Lett. 170:370-374(1984)
    Cited for: DISULFIDE BONDS.
  6. "The cysteine proteinase inhibitor chicken cystatin is a phosphoprotein."
    Laber B., Krieglstein K., Henschen A., Kos J., Turk V., Huber R., Bode W.
    FEBS Lett. 248:162-168(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.
  7. "The 2.0 A X-ray crystal structure of chicken egg white cystatin and its possible mode of interaction with cysteine proteinases."
    Bode W., Engh R., Musil D., Thiele U., Huber R., Karshikov A., Brzin J., Kos J., Turk V.
    EMBO J. 7:2593-2599(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
  8. "The structures of native phosphorylated chicken cystatin and of a recombinant unphosphorylated variant in solution."
    Dieckmann T., Mitschang L., Hofmann M., Kos J., Turk V., Auerswald E.A., Jeanicke R., Oschkinat H.
    J. Mol. Biol. 234:1048-1059(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiCYT_CHICK
AccessioniPrimary (citable) accession number: P01038
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 1, 1989
Last modified: June 8, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.