P01023Q13677Q59F47Q5QTS0Q68DN2Q6PIY3Q6PN97A2MG_HUMANAlpha-2-macroglobulinAlpha-2-MC3 and PZP-like alpha-2-macroglobulin domain-containing protein 5A2MCPAMD5FWP007Homo sapiensHumanEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomoNucleotide sequence of cDNA encoding human alpha 2-macroglobulin and assignment of the chromosomal locus.NUCLEOTIDE SEQUENCE [MRNA]VARIANTS ASP-639 AND VAL-1000Alpha(2) macroglobulin, a PSA-binding protein, is expressed in human prostate stroma.NUCLEOTIDE SEQUENCE [MRNA]VARIANTS ASP-639 AND VAL-1000NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]VARIANT ASP-639The full-ORF clone resource of the German cDNA consortium.NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]VARIANTS ASP-639 AND VAL-1000The finished DNA sequence of human chromosome 12.NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]VARIANTS ASP-639 AND VAL-1000Structure of the human alpha-2 macroglobulin gene and its promotor.NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29Primary structure of human alpha 2-macroglobulin. V. The complete structure.PROTEIN SEQUENCE OF 24-1474SUBUNITSUBCELLULAR LOCATIONTISSUE SPECIFICITYDISULFIDE BONDSERRATUM OF PUBMED:6203908Primary structure of human alpha 2-macroglobulin. Complete disulfide bridge assignment and localization of two interchain bridges in the dimeric proteinase binding unit.PROTEIN SEQUENCE OF 273-286 AND 426-436DISULFIDE BONDSA genetic polymorphism in a functional domain of human pregnancy zone protein: the bait region. Genomic structure of the bait domains of human pregnancy zone protein and alpha 2 macroglobulin.PROTEIN SEQUENCE OF 672-747Cloning of the human alpha 2-macroglobulin gene and detection of mutations in two functional domains: the bait region and the thiolester site.NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 672-746VARIANT TYR-972Human alpha 2-macroglobulin gene is located on chromosome 12.NUCLEOTIDE SEQUENCE [MRNA] OF 832-1474NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1208-1474Proteolytic cleavage sites on alpha 2-macroglobulin resulting in proteinase binding are different for trypsin and Staphylococcus aureus V-8 proteinase.INHIBITORY SITEPrimary structure of the 'bait' region for proteinases in alpha 2-macroglobulin. Nature of the complex.INHIBITORY SITEPrimary and secondary cleavage sites in the bait region of alpha 2-macroglobulin.INHIBITORY SITEHuman neutrophil elastase and cathepsin G cleavage sites in the bait region of alpha 2-macroglobulin. Proposed structural limits of the bait region.INHIBITORY SITEIdentification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry.GLYCOSYLATION AT ASN-991Screening for N-glycosylated proteins by liquid chromatography mass spectrometry.GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-869 AND ASN-1424Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-55; ASN-247; ASN-396; ASN-410; ASN-869; ASN-991 AND ASN-1424Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry.GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-396; ASN-991 AND ASN-1424A strategy for precise and large scale identification of core fucosylated glycoproteins.GLYCOSYLATION AT ASN-55 AND ASN-1424Initial characterization of the human central proteome.IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]Localization of basic residues required for receptor binding to the single alpha-helix of the receptor binding domain of human alpha2-macroglobulin.STRUCTURE BY NMR OF 1337-1474Human alpha2-macroglobulin is composed of multiple domains, as predicted by homology with complement component C3.X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 126-227DOMAIN STRUCTURESequence polymorphism in the human alpha2-macroglobulin (A2M) gene.VARIANT VAL-1000Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.Homotetramer; disulfide-linked.P01023Q6PCB6false3P01023P63010-2false3P01023Q9UII2false3P01023Q8WUW1false3P01023Q92478false3P01023Q9UNS2false3P01023P04141false3P01023P35222false3P01023Q7L576false3P01023Q9NR90-2false3P01023Q9UHI6false3P01023Q9H147false3P01023O75616false3P01023Q9Y261-2false3P01023Q6PIV2false3P01023Q9ULV1false3P01023P0C0S5false3P01023P68431false3P01023P09017false3P01023Q6DN90-2false3P01023Q92993false3P01023Q92993-2false3P01023P60409false3P01023Q8IUC2false3P01023Q9UPM6false3P01023Q8TBB1false3P01023P07948false3P01023Q8TDB4false3P01023A4FUJ8false3P01023P41218false3P01023Q9Y605false3P01023Q96FW1false3P01023Q6GQQ9-2false3P01023Q99497false3P01023O75925false3P01023P27986-2false3P01023O75626-3false3P01023P57729false3P01023Q9ULX5false3P01023Q96D59false3P01023P04271false3P01023Q16637-3false3P01023Q7Z6I5false3P01023Q9UNE7false3P01023O75558false3P01023Q8N4C7false3P01023P43405-2false3P01023P28347-2false3P01023Q86WT6-2false3P01023Q8WVJ9false3P01023P10599false3P01023Q9BVJ6false3P01023Q9UBQ0-2false3P01023Q96AX1false3P01023Q8N895false3P01023Q99KR7true3SecretedSecreted in plasma.Unlike the rat protein, which is an acute phase protein, this protein is always in circulation at high levels.Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.Extended N-terminus.Extended N-terminus.Alpha-2 macroglobulin entry3D-structureBait regionDirect protein sequencingDisulfide bondGlycoproteinIsopeptide bondProtease inhibitorReference proteomeSecretedSerine protease inhibitorSignalThioester bondNDRHLQCYIVDVLSFVACCSCEAVVMADHDMGKNKLLHPSLVLLLLVLLPTDASVSGKPQYMVLVPSLLHTETTEKGCVLLSYLNETVTVSASLESVRGNRSLFTDLEAENDVLHCVAFAVPKSSSNEEVMFLTVQVKGPTQEFKKRTTVMVKNEDSLVFVQTDKSIYKPGQTVKFRVVSMDENFHPLNELIPLVYIQDPKGNRIAQWQSFQLEGGLKQFSFPLSSEPFQGSYKVVVQKKSGGRTEHPFTVEEFVLPKFEVQVTVPKIITILEEEMNVSVCGLYTYGKPVPGHVTVSICRKYSDASDCHGEDSQAFCEKFSGQLNSHGCFYQQVKTKVFQLKRKEYEMKLHTEAQIQEEGTVVELTGRQSSEITRTITKLSFVKVDSHFRQGIPFFGQVRLVDGKGVPIPNKVIFIRGNEANYYSNATTDEHGLVQFSINTTNVMGTSLTVRVNYKDRSPCYGYQWVSEEHEEAHHTAYLVFSPSKSFVHLEPMSHELPCGHTQTVQAHYILNGGTLLGLKKLSFYYLIMAKGGIVRTGTHGLLVKQEDMKGHFSISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDVENCLANKVDLSFSPSQSLPASHAHLRVTAAPQSVCALRAVDQSVLLMKPDAELSASSVYNLLPEKDLTGFPGPLNDQDNEDCINRHNVYINGITYTPVSSTNEKDMYSFLEDMGLKAFTNSKIRKPKMCPQLQQYEMHGPEGLRVGFYESDVMGRGHARLVHVEEPHTETVRKYFPETWIWDLVVVNSAGVAEVGVTVPDTITEWKAGAFCLSEDAGLGISSTASLRAFQPFFVELTMPYSVIRGEAFTLKATVLNYLPKCIRVSVQLEASPAFLAVPVEKEQAPHCICANGRQTVSWAVTPKSLGNVNFTVSAEALESQELCGTEVPSVPEHGRKDTVIKPLLVEPEGLEKETTFNSLLCPSGGEVSEELSLKLPPNVVEESARASVSVLGDILGSAMQNTQNLLQMPYGCGEQNMVLFAPNIYVLDYLNETQQLTPEIKSKAIGYLNTGYQRQLNYKHYDGSYSTFGERYGRNQGNTWLTAFVLKTFAQARAYIFIDEAHITQALIWLSQRQKDNGCFRSSGSLLNNAIKGGVEDEVTLSAYITIALLEIPLTVTHPVVRNALFCLESAWKTAQEGDHGSHVYTKALLAYAFALAGNQDKRKEVLKSLNEEAVKKDNSVHWERPQKPKAPVGHFYEPQAPSAEVEMTSYVLLAYLTAQPAPTSEDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHALSKYGAATFTRTGKAAQVTIQSSGTFSSKFQVDNNNRLLLQQVSLPELPGEYSMKVTGEGCVYLQTSLKYNILPEKEEFPFALGVQTLPQTCDEPKAHTSFQISLSVSYTGSRSASNMAIVDVKMVSGFIPLKPTVKMLERSNHVSRTEVSSNHVLIYLDKVSNQTLSLFFTVLQDVPVRDLKPAIVKVYDYYETDEFAIAEYNAPCSKDLGNA
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