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Protein

Angiotensinogen

Gene

Agt

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.By similarity
Angiotensin-2: acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.By similarity
Angiotensin-3: stimulates aldosterone release.By similarity
Angiotensin 1-7: is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.By similarity1 Publication

GO - Molecular functioni

  • angiotensin receptor binding Source: RGD
  • hormone activity Source: BHF-UCL
  • receptor agonist activity Source: BHF-UCL
  • serine-type endopeptidase inhibitor activity Source: GO_Central
  • type 1 angiotensin receptor binding Source: RGD
  • type 2 angiotensin receptor binding Source: RGD

GO - Biological processi

  • activation of phospholipase C activity Source: RGD
  • aging Source: RGD
  • angiotensin-activated signaling pathway Source: BHF-UCL
  • angiotensin-mediated drinking behavior Source: RGD
  • artery smooth muscle contraction Source: RGD
  • catenin import into nucleus Source: RGD
  • cell growth involved in cardiac muscle cell development Source: RGD
  • cell surface receptor signaling pathway Source: RGD
  • cellular response to mechanical stimulus Source: RGD
  • cellular sodium ion homeostasis Source: RGD
  • cytokine secretion Source: RGD
  • ERK1 and ERK2 cascade Source: RGD
  • female pregnancy Source: RGD
  • fibroblast proliferation Source: RGD
  • G-protein coupled receptor signaling pathway Source: RGD
  • kidney development Source: Ensembl
  • negative regulation of angiogenesis Source: RGD
  • negative regulation of cell growth Source: RGD
  • negative regulation of neurotrophin TRK receptor signaling pathway Source: Ensembl
  • negative regulation of tissue remodeling Source: RGD
  • positive regulation of activation of JAK2 kinase activity Source: Ensembl
  • positive regulation of blood pressure Source: RGD
  • positive regulation of branching involved in ureteric bud morphogenesis Source: Ensembl
  • positive regulation of cardiac muscle cell apoptotic process Source: RGD
  • positive regulation of cardiac muscle hypertrophy Source: BHF-UCL
  • positive regulation of catalytic activity Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of cholesterol esterification Source: Ensembl
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • positive regulation of endothelial cell migration Source: Ensembl
  • positive regulation of epidermal growth factor receptor signaling pathway Source: Ensembl
  • positive regulation of extracellular matrix constituent secretion Source: RGD
  • positive regulation of extrinsic apoptotic signaling pathway Source: Ensembl
  • positive regulation of fibroblast proliferation Source: BHF-UCL
  • positive regulation of gap junction assembly Source: Ensembl
  • positive regulation of glucose import in response to insulin stimulus Source: RGD
  • positive regulation of L-arginine import into cell Source: RGD
  • positive regulation of L-lysine import into cell Source: RGD
  • positive regulation of nitric oxide biosynthetic process Source: RGD
  • positive regulation of phosphatidylinositol 3-kinase signaling Source: Ensembl
  • positive regulation of protein tyrosine kinase activity Source: Ensembl
  • positive regulation of renal sodium excretion Source: RGD
  • positive regulation of superoxide anion generation Source: RGD
  • positive regulation of transcription, DNA-templated Source: BHF-UCL
  • positive regulation of vascular associated smooth muscle cell migration Source: RGD
  • positive regulation of vascular smooth muscle cell proliferation Source: RGD
  • positive regulation of vasodilation Source: RGD
  • regulation of apoptotic process Source: RGD
  • regulation of calcium ion transport Source: RGD
  • regulation of cardiac conduction Source: Ensembl
  • regulation of extracellular matrix assembly Source: Ensembl
  • regulation of heart rate Source: RGD
  • regulation of long-term neuronal synaptic plasticity Source: RGD
  • regulation of norepinephrine secretion Source: RGD
  • regulation of renal sodium excretion Source: RGD
  • regulation of transmission of nerve impulse Source: RGD
  • renin-angiotensin regulation of aldosterone production Source: RGD
  • response to muscle activity involved in regulation of muscle adaptation Source: BHF-UCL
  • smooth muscle cell proliferation Source: RGD
  • stress-activated MAPK cascade Source: RGD
  • uterine smooth muscle contraction Source: RGD
  • vasoconstriction Source: RGD
  • vasodilation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Vasoactive, Vasoconstrictor

Enzyme and pathway databases

ReactomeiR-RNO-2022377. Metabolism of Angiotensinogen to Angiotensins.
R-RNO-375276. Peptide ligand-binding receptors.
R-RNO-416476. G alpha (q) signalling events.
R-RNO-418594. G alpha (i) signalling events.

Protein family/group databases

MEROPSiI04.953.

Names & Taxonomyi

Protein namesi
Recommended name:
Angiotensinogen
Alternative name(s):
Serpin A8
Cleaved into the following 8 chains:
Alternative name(s):
Angiotensin 1-10
Angiotensin I
Short name:
Ang I
Alternative name(s):
Angiotensin 1-8
Angiotensin II
Short name:
Ang II
Alternative name(s):
Angiotensin 2-8
Angiotensin III
Short name:
Ang III
Des-Asp[1]-angiotensin II
Alternative name(s):
Angiotensin 3-8
Angiotensin IV
Short name:
Ang IV
Gene namesi
Name:Agt
Synonyms:Serpina8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi2069. Agt.

Subcellular locationi

GO - Cellular componenti

  • blood microparticle Source: Ensembl
  • cytoplasm Source: RGD
  • extracellular exosome Source: Ensembl
  • extracellular space Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 241 PublicationAdd BLAST24
ChainiPRO_000003246825 – 477AngiotensinogenAdd BLAST453
PeptideiPRO_000003246925 – 34Angiotensin-110
PeptideiPRO_000042067425 – 33Angiotensin 1-99
PeptideiPRO_000003247025 – 32Angiotensin-28
PeptideiPRO_000042067525 – 31Angiotensin 1-77
PeptideiPRO_000042067625 – 29Angiotensin 1-55
PeptideiPRO_000042067725 – 28Angiotensin 1-44
PeptideiPRO_000003247126 – 32Angiotensin-37
PeptideiPRO_000042067827 – 32Angiotensin-46

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi42 ↔ 1611 Publication
Glycosylationi295N-linked (GlcNAc...)Sequence analysis1
Glycosylationi319N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

In response to low blood pressure, the enzyme renin/REN cleaves angiotensinogen to produce angiotensin-1. Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4 (By similarity). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2 and can be further processed by ACE to produce angiotensin 1-7, angiotensin 1-5 and angiotensin 1-4. Angiotensin 1-7 has also been proposed to be cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin) (By similarity).By similarity
The disulfide bond is labile. Angiotensinogen is present in the circulation in a near 40:60 ratio with the oxidized disulfide-bonded form, which preferentially interacts with receptor-bound renin.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP01015.
PRIDEiP01015.

PTM databases

iPTMnetiP01015.
PhosphoSitePlusiP01015.

Expressioni

Tissue specificityi

Expressed by the liver and secreted in plasma.

Gene expression databases

BgeeiENSRNOG00000018445.
GenevisibleiP01015. RN.

Interactioni

GO - Molecular functioni

  • angiotensin receptor binding Source: RGD
  • hormone activity Source: BHF-UCL
  • receptor agonist activity Source: BHF-UCL
  • type 1 angiotensin receptor binding Source: RGD
  • type 2 angiotensin receptor binding Source: RGD

Protein-protein interaction databases

MINTiMINT-1522474.
STRINGi10116.ENSRNOP00000024917.

Chemistry databases

BindingDBiP01015.

Structurei

Secondary structure

1477
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni33 – 35Combined sources3
Helixi39 – 43Combined sources5
Helixi72 – 84Combined sources13
Helixi88 – 115Combined sources28
Beta strandi120 – 124Combined sources5
Helixi126 – 138Combined sources13
Helixi142 – 151Combined sources10
Helixi167 – 181Combined sources15
Beta strandi193 – 203Combined sources11
Helixi211 – 216Combined sources6
Turni217 – 220Combined sources4
Beta strandi224 – 228Combined sources5
Beta strandi231 – 233Combined sources3
Helixi235 – 250Combined sources16
Beta strandi267 – 279Combined sources13
Beta strandi282 – 284Combined sources3
Beta strandi289 – 294Combined sources6
Beta strandi297 – 301Combined sources5
Beta strandi303 – 315Combined sources13
Turni316 – 319Combined sources4
Beta strandi320 – 340Combined sources21
Helixi341 – 343Combined sources3
Helixi344 – 351Combined sources8
Beta strandi353 – 355Combined sources3
Beta strandi366 – 373Combined sources8
Beta strandi375 – 382Combined sources8
Helixi383 – 389Combined sources7
Turni393 – 396Combined sources4
Helixi403 – 405Combined sources3
Beta strandi406 – 408Combined sources3
Beta strandi416 – 425Combined sources10
Beta strandi452 – 458Combined sources7
Turni459 – 462Combined sources4
Beta strandi463 – 471Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SMRX-ray2.00B/D/F/H30-33[»]
2WXZX-ray2.80A/C25-477[»]
2WY1X-ray3.15A/B25-477[»]
ProteinModelPortaliP01015.
SMRiP01015.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01015.

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
GeneTreeiENSGT00850000132337.
HOGENOMiHOG000033941.
HOVERGENiHBG004233.
InParanoidiP01015.
KOiK09821.
OMAiRFMQAVT.
OrthoDBiEOG091G077M.
PhylomeDBiP01015.
TreeFamiTF343201.

Family and domain databases

InterProiIPR000227. Angiotensinogen.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 2 hits.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
PRINTSiPR00654. ANGIOTENSNGN.
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01015-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPTGAGLKA TIFCILTWVS LTAGDRVYIH PFHLLYYSKS TCAQLENPSV
60 70 80 90 100
ETLPEPTFEP VPIQAKTSPV DEKTLRDKLV LATEKLEAED RQRAAQVAMI
110 120 130 140 150
ANFMGFRMYK MLSEARGVAS GAVLSPPALF GTLVSFYLGS LDPTASQLQV
160 170 180 190 200
LLGVPVKEGD CTSRLDGHKV LTALQAVQGL LVTQGGSSSQ TPLLQSTVVG
210 220 230 240 250
LFTAPGLRLK QPFVESLGPF TPAIFPRSLD LSTDPVLAAQ KINRFVQAVT
260 270 280 290 300
GWKMNLPLEG VSTDSTLFFN TYVHFQGKMR GFSQLTGLHE FWVDNSTSVS
310 320 330 340 350
VPMLSGTGNF QHWSDAQNNF SVTRVPLGES VTLLLIQPQC ASDLDRVEVL
360 370 380 390 400
VFQHDFLTWI KNPPPRAIRL TLPQLEIRGS YNLQDLLAQA KLSTLLGAEA
410 420 430 440 450
NLGKMGDTNP RVGEVLNSIL LELQAGEEEQ PTESAQQPGS PEVLDVTLSS
460 470
PFLFAIYERD SGALHFLGRV DNPQNVV
Length:477
Mass (Da):51,982
Last modified:July 21, 1986 - v1
Checksum:i689051A5788D693D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00094
, L00091, L00092, L00093 Genomic DNA. Translation: AAA98779.1.
BC078741 mRNA. Translation: AAH78741.1.
BC087679 mRNA. Translation: AAH87679.1.
PIRiA93945. ANRT.
RefSeqiNP_602308.1. NM_134432.2.
XP_008770819.1. XM_008772597.2.
UniGeneiRn.6319.

Genome annotation databases

EnsembliENSRNOT00000024917; ENSRNOP00000024917; ENSRNOG00000018445.
GeneIDi24179.
KEGGirno:24179.
UCSCiRGD:2069. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00094
, L00091, L00092, L00093 Genomic DNA. Translation: AAA98779.1.
BC078741 mRNA. Translation: AAH78741.1.
BC087679 mRNA. Translation: AAH87679.1.
PIRiA93945. ANRT.
RefSeqiNP_602308.1. NM_134432.2.
XP_008770819.1. XM_008772597.2.
UniGeneiRn.6319.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SMRX-ray2.00B/D/F/H30-33[»]
2WXZX-ray2.80A/C25-477[»]
2WY1X-ray3.15A/B25-477[»]
ProteinModelPortaliP01015.
SMRiP01015.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1522474.
STRINGi10116.ENSRNOP00000024917.

Chemistry databases

BindingDBiP01015.

Protein family/group databases

MEROPSiI04.953.

PTM databases

iPTMnetiP01015.
PhosphoSitePlusiP01015.

Proteomic databases

PaxDbiP01015.
PRIDEiP01015.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024917; ENSRNOP00000024917; ENSRNOG00000018445.
GeneIDi24179.
KEGGirno:24179.
UCSCiRGD:2069. rat.

Organism-specific databases

CTDi183.
RGDi2069. Agt.

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
GeneTreeiENSGT00850000132337.
HOGENOMiHOG000033941.
HOVERGENiHBG004233.
InParanoidiP01015.
KOiK09821.
OMAiRFMQAVT.
OrthoDBiEOG091G077M.
PhylomeDBiP01015.
TreeFamiTF343201.

Enzyme and pathway databases

ReactomeiR-RNO-2022377. Metabolism of Angiotensinogen to Angiotensins.
R-RNO-375276. Peptide ligand-binding receptors.
R-RNO-416476. G alpha (q) signalling events.
R-RNO-418594. G alpha (i) signalling events.

Miscellaneous databases

EvolutionaryTraceiP01015.
PROiP01015.

Gene expression databases

BgeeiENSRNOG00000018445.
GenevisibleiP01015. RN.

Family and domain databases

InterProiIPR000227. Angiotensinogen.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 2 hits.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
PRINTSiPR00654. ANGIOTENSNGN.
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiANGT_RAT
AccessioniPrimary (citable) accession number: P01015
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.