Angiotensinogen precursor - Rattus norvegicus (Rat)

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P01015 (ANGT_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Angiotensinogen

Alternative name(s):
Serpin A8

Cleaved into the following 8 chains:

Angiotensin-1
Alternative name(s):
Angiotensin 1-10
Angiotensin I
Short name=Ang I
Angiotensin-2
Alternative name(s):
Angiotensin 1-8
Angiotensin II
Short name=Ang II
Angiotensin-3
Alternative name(s):
Angiotensin 2-8
Angiotensin III
Short name=Ang III
Des-Asp[1]-angiotensin II
Angiotensin-4
Alternative name(s):
Angiotensin 3-8
Angiotensin IV
Short name=Ang IV
Angiotensin 1-9
Angiotensin 1-7
Angiotensin 1-5
Angiotensin 1-4

Gene names

Name:	Agt
Synonyms:	Serpina8

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis By similarity. Ref.5 Angiotensin-2: acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone By similarity. Ref.5 Angiotensin-3: stimulates aldosterone release By similarity. Ref.5 Angiotensin 1-7: is a ligand for the G-protein coupled receptor MAS1 By similarity. Has vasodilator and antidiuretic effects By similarity. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets. Ref.5
Subcellular location	Secreted.
Tissue specificity	Expressed by the liver and secreted in plasma.
Post-translational modification	In response to low blood pressure, the enzyme renin/REN cleaves angiotensinogen to produce angiotensin-1. Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4 By similarity. Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2 and can be further processed by ACE to produce angiotensin 1-7, angiotensin 1-5 and angiotensin 1-4. Angiotensin 1-7 has also been proposed to be cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin) By similarity. The disulfide bond is labile. Angiotensinogen is present in the circulation in a near 40:60 ratio with the oxidized disulfide-bonded form, which preferentially interacts with receptor-bound renin.
Sequence similarities	Belongs to the serpin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Vasoactive Vasoconstrictor
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ERK1 and ERK2 cascade Inferred from direct assay PubMed 21367774. Source: RGD activation of NF-kappaB-inducing kinase activity Inferred from electronic annotation. Source: Compara activation of phospholipase C activity Inferred from mutant phenotype PubMed 11100981. Source: RGD aging Inferred from expression pattern PubMed 21318332. Source: RGD angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure Inferred from electronic annotation. Source: Compara angiotensin-mediated drinking behavior Inferred from direct assay PubMed 21281609. Source: RGD artery smooth muscle contraction Inferred from direct assay PubMed 21076237. Source: RGD astrocyte activation Inferred from electronic annotation. Source: Compara blood vessel development Inferred from electronic annotation. Source: Compara branching involved in ureteric bud morphogenesis Inferred from electronic annotation. Source: Compara catenin import into nucleus Inferred from direct assay PubMed 21376054. Source: RGD cell growth involved in cardiac muscle cell development Inferred from direct assay PubMed 21376054. Source: RGD cell-matrix adhesion Inferred from electronic annotation. Source: Compara cellular response to mechanical stimulus Inferred from expression pattern PubMed 21131638. Source: RGD cellular sodium ion homeostasis Inferred from mutant phenotype PubMed 16380540. Source: RGD cytokine secretion Inferred from direct assay PubMed 21282555. Source: RGD establishment of blood-nerve barrier Inferred from electronic annotation. Source: Compara excretion Inferred from electronic annotation. Source: Compara extracellular matrix organization Inferred from electronic annotation. Source: Compara fibroblast proliferation Inferred from direct assay PubMed 21419085. Source: RGD hormone metabolic process Inferred from electronic annotation. Source: Compara induction of apoptosis Inferred from direct assay PubMed 21102479. Source: RGD kidney development Inferred from electronic annotation. Source: Compara negative regulation of angiogenesis Inferred from direct assay PubMed 21281454. Source: RGD negative regulation of cell growth Inferred from mutant phenotype PubMed 15951342. Source: RGD negative regulation of cell proliferation Inferred from electronic annotation. Source: Compara negative regulation of nerve growth factor receptor signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of neuron apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of tissue remodeling Inferred from direct assay PubMed 21167013. Source: RGD ovarian follicle rupture Inferred from electronic annotation. Source: Compara peristalsis Inferred from electronic annotation. Source: Compara positive regulation of MAPK cascade Inferred from electronic annotation. Source: Compara positive regulation of activation of JAK2 kinase activity Inferred from electronic annotation. Source: Compara positive regulation of blood pressure Inferred from direct assay PubMed 21318332. Source: RGD positive regulation of branching involved in ureteric bud morphogenesis Inferred from electronic annotation. Source: Compara positive regulation of cardiac muscle hypertrophy Inferred from direct assay PubMed 8348686. Source: BHF-UCL positive regulation of cholesterol esterification Inferred from electronic annotation. Source: Compara positive regulation of endothelial cell migration Inferred from electronic annotation. Source: Compara positive regulation of epidermal growth factor receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of extracellular matrix constituent secretion Inferred from direct assay PubMed 21419085. Source: RGD positive regulation of fatty acid biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of fibroblast proliferation Inferred from direct assay PubMed 8348686. Source: BHF-UCL positive regulation of multicellular organism growth Inferred from electronic annotation. Source: Compara positive regulation of organ growth Inferred from electronic annotation. Source: Compara positive regulation of phosphatidylinositol 3-kinase cascade Inferred from electronic annotation. Source: Compara positive regulation of protein tyrosine kinase activity Inferred from electronic annotation. Source: Compara positive regulation of renal sodium excretion Inferred from mutant phenotype PubMed 16380540. Source: RGD positive regulation of superoxide anion generation Inferred from direct assay PubMed 21376054. Source: RGD positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara regulation of calcium ion transport Inferred from direct assay PubMed 21423294. Source: RGD regulation of inflammatory response Inferred from electronic annotation. Source: Compara regulation of long-term neuronal synaptic plasticity Inferred from mutant phenotype PubMed 11282364. Source: RGD regulation of norepinephrine secretion Inferred from mutant phenotype PubMed 11100981. Source: RGD regulation of proteolysis Inferred from Biological aspect of Ancestor. Source: RefGenome regulation of renal output by angiotensin Inferred from electronic annotation. Source: Compara renal response to blood flow involved in circulatory renin-angiotensin regulation of systemic arterial blood pressure Inferred from electronic annotation. Source: Compara renin-angiotensin regulation of aldosterone production Inferred from direct assay PubMed 21318332. Source: RGD response to cold Inferred from electronic annotation. Source: Compara response to muscle activity involved in regulation of muscle adaptation Inferred from mutant phenotype PubMed 8252633. Source: BHF-UCL response to salt stress Inferred from electronic annotation. Source: Compara smooth muscle cell differentiation Inferred from electronic annotation. Source: Compara smooth muscle cell proliferation Inferred from direct assay PubMed 21292788. Source: RGD stress-activated MAPK cascade Inferred from direct assay PubMed 21367774. Source: RGD uterine smooth muscle contraction Inferred from direct assay PubMed 21401609. Source: RGD vasodilation Inferred from direct assay PubMed 21056585. Source: RGD
Cellular_component	cytoplasmic part Inferred from direct assay PubMed 12242043. Source: RGD extracellular space Inferred from direct assay PubMed 8252633. Source: BHF-UCL
Molecular_function	acetyltransferase activator activity Inferred from electronic annotation. Source: Compara alpha1-adrenergic receptor activity Traceable author statement PubMed 9931144. Source: RGD hormone activity Inferred from direct assay PubMed 8348686. Source: BHF-UCL serine-type endopeptidase inhibitor activity Inferred from Biological aspect of Ancestor. Source: RefGenome type 1 angiotensin receptor binding Inferred from mutant phenotype PubMed 16286564. Source: RGD type 2 angiotensin receptor binding Inferred from mutant phenotype PubMed 16286564. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

Ref.4

Chain

25 – 477

453

Angiotensinogen

PRO_0000032468

Peptide

25 – 34

Angiotensin-1 Ref.4

PRO_0000032469

Peptide

25 – 33

Angiotensin 1-9

PRO_0000420674

Peptide

25 – 32

Angiotensin-2

PRO_0000032470

Peptide

25 – 31

Angiotensin 1-7

PRO_0000420675

Peptide

25 – 29

Angiotensin 1-5

PRO_0000420676

Peptide

25 – 28

Angiotensin 1-4

PRO_0000420677

Peptide

26 – 32

Angiotensin-3

PRO_0000032471

Peptide

27 – 32

Angiotensin-4

PRO_0000420678

Amino acid modifications

Glycosylation

295

N-linked (GlcNAc...) Potential

Glycosylation

319

N-linked (GlcNAc...) Potential

Disulfide bond

42 ↔ 161

Ref.6

Secondary structure

477

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P01015 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 689051A5788D693D
Show »

FASTA

477

51,982

        10         20         30         40         50         60 
MTPTGAGLKA TIFCILTWVS LTAGDRVYIH PFHLLYYSKS TCAQLENPSV ETLPEPTFEP 

        70         80         90        100        110        120 
VPIQAKTSPV DEKTLRDKLV LATEKLEAED RQRAAQVAMI ANFMGFRMYK MLSEARGVAS 

       130        140        150        160        170        180 
GAVLSPPALF GTLVSFYLGS LDPTASQLQV LLGVPVKEGD CTSRLDGHKV LTALQAVQGL 

       190        200        210        220        230        240 
LVTQGGSSSQ TPLLQSTVVG LFTAPGLRLK QPFVESLGPF TPAIFPRSLD LSTDPVLAAQ 

       250        260        270        280        290        300 
KINRFVQAVT GWKMNLPLEG VSTDSTLFFN TYVHFQGKMR GFSQLTGLHE FWVDNSTSVS 

       310        320        330        340        350        360 
VPMLSGTGNF QHWSDAQNNF SVTRVPLGES VTLLLIQPQC ASDLDRVEVL VFQHDFLTWI 

       370        380        390        400        410        420 
KNPPPRAIRL TLPQLEIRGS YNLQDLLAQA KLSTLLGAEA NLGKMGDTNP RVGEVLNSIL 

       430        440        450        460        470 
LELQAGEEEQ PTESAQQPGS PEVLDVTLSS PFLFAIYERD SGALHFLGRV DNPQNVV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequence analysis of cDNA for rat angiotensinogen." Ohkubo H., Kageyama R., Ujihara M., Hirose T., Inayama S., Nakanishi S. Proc. Natl. Acad. Sci. U.S.A. 80:2196-2200(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.
[2]	"Common structural organization of the angiotensinogen and the alpha 1-antitrypsin genes." Tanaka T., Ohkubo H., Nakanishi S. J. Biol. Chem. 259:8063-8065(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Kidney.
[4]	"Comparative studies on angiotensins. II. Structure of rat angiotensin and its identification by DNS-method." Nakayama T., Nakajima T., Sokabe H. Chem. Pharm. Bull. 20:1579-1581(1972) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-34.
[5]	"The antithrombotic effect of angiotensin-(1-7) involves mas-mediated NO release from platelets." Fraga-Silva R.A., Pinheiro S.V.B., Goncalves A.C., Alenina N., Bader M., Santos R.A.S. Mol. Med. 14:28-35(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF ANGIOTENSIN 1-7.
[6]	"A redox switch in angiotensinogen modulates angiotensin release." Zhou A., Carrell R.W., Murphy M.P., Wei Z., Yan Y., Stanley P.L., Stein P.E., Broughton Pipkin F., Read R.J. Nature 468:108-111(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 25-477, DISULFIDE BOND.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L00094

L00093 Genomic DNA. Translation: AAA98779.1.
BC078741 mRNA. Translation: AAH78741.1.
BC087679 mRNA. Translation: AAH87679.1.

IPI

IPI00209744.

PIR

ANRT. A93945.

RefSeq

NP_602308.1. NM_134432.2.

UniGene

Rn.6319.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SMR	X-ray	2.00	B/D/F/H	30-33	[»]
2WXZ	X-ray	2.80	A/C	25-477	[»]
2WY1	X-ray	3.15	A/B	25-477	[»]

ProteinModelPortal

P01015.

ModBase

Search...

Protein-protein interaction databases

STRING

10116.ENSRNOP00000024917.

Protein family/group databases

MEROPS

I04.953.

PTM databases

PhosphoSite

P01015.

Proteomic databases

PaxDb

P01015.

PRIDE

P01015.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000024917; ENSRNOP00000024917; ENSRNOG00000018445.

GeneID

24179.

KEGG

rno:24179.

UCSC

RGD:2069. rat.

Organism-specific databases

CTD

183.

RGD

2069. Agt.

Phylogenomic databases

eggNOG

COG4826.

GeneTree

ENSGT00690000102272.

HOGENOM

HOG000033941.

HOVERGEN

HBG004233.

InParanoid

P01015.

K09821.

OrthoDB

EOG44TP89.

Gene expression databases

Genevestigator

P01015.

GermOnline

ENSRNOG00000018445. Rattus norvegicus.

Family and domain databases

InterPro

IPR000227. Angiotensngn.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]

PANTHER

PTHR11461. PTHR11461. 1 hit.

Pfam

PF00079. Serpin. 1 hit.
[Graphical view]

PRINTS

PR00654. ANGIOTENSNGN.

SMART

SM00093. SERPIN. 1 hit.
[Graphical view]

SUPFAM

SSF56574. Prot_inh_serpin. 1 hit.

PROSITE

PS00284. SERPIN. False negative.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P01015.

EvolutionaryTrace

P01015.

NextBio

602521.

Entry information

Entry name

ANGT_RAT

Accession

Primary (citable) accession number: P01015

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following 8 chains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents