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P01015

- ANGT_RAT

UniProt

P01015 - ANGT_RAT

Protein

Angiotensinogen

Gene

Agt

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.By similarity
    Angiotensin-2: acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.By similarity
    Angiotensin-3: stimulates aldosterone release.By similarity
    Angiotensin 1-7: is a ligand for the G-protein coupled receptor MAS1 By similarity. Has vasodilator and antidiuretic effects By similarity. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.By similarity1 Publication

    GO - Molecular functioni

    1. acetyltransferase activator activity Source: Ensembl
    2. angiotensin receptor binding Source: RGD
    3. hormone activity Source: BHF-UCL
    4. serine-type endopeptidase inhibitor activity Source: RefGenome
    5. type 1 angiotensin receptor binding Source: RGD
    6. type 2 angiotensin receptor binding Source: RGD

    GO - Biological processi

    1. activation of NF-kappaB-inducing kinase activity Source: Ensembl
    2. activation of phospholipase C activity Source: RGD
    3. aging Source: RGD
    4. angiotensin-mediated drinking behavior Source: RGD
    5. angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure Source: Ensembl
    6. artery smooth muscle contraction Source: RGD
    7. astrocyte activation Source: Ensembl
    8. blood vessel development Source: Ensembl
    9. branching involved in ureteric bud morphogenesis Source: Ensembl
    10. catenin import into nucleus Source: RGD
    11. cell growth involved in cardiac muscle cell development Source: RGD
    12. cell-matrix adhesion Source: Ensembl
    13. cell surface receptor signaling pathway Source: RGD
    14. cellular response to mechanical stimulus Source: RGD
    15. cellular sodium ion homeostasis Source: RGD
    16. cytokine secretion Source: RGD
    17. ERK1 and ERK2 cascade Source: RGD
    18. establishment of blood-nerve barrier Source: Ensembl
    19. excretion Source: Ensembl
    20. extracellular matrix organization Source: Ensembl
    21. female pregnancy Source: RGD
    22. fibroblast proliferation Source: RGD
    23. G-protein coupled receptor signaling pathway Source: RGD
    24. hormone metabolic process Source: Ensembl
    25. kidney development Source: Ensembl
    26. negative regulation of angiogenesis Source: RGD
    27. negative regulation of cell growth Source: RGD
    28. negative regulation of cell proliferation Source: Ensembl
    29. negative regulation of endopeptidase activity Source: RefGenome
    30. negative regulation of neuron apoptotic process Source: Ensembl
    31. negative regulation of neurotrophin TRK receptor signaling pathway Source: Ensembl
    32. negative regulation of tissue remodeling Source: RGD
    33. ovarian follicle rupture Source: Ensembl
    34. peristalsis Source: Ensembl
    35. positive regulation of activation of JAK2 kinase activity Source: Ensembl
    36. positive regulation of blood pressure Source: RGD
    37. positive regulation of branching involved in ureteric bud morphogenesis Source: Ensembl
    38. positive regulation of cardiac muscle hypertrophy Source: BHF-UCL
    39. positive regulation of catalytic activity Source: RGD
    40. positive regulation of cell proliferation Source: RGD
    41. positive regulation of cholesterol esterification Source: Ensembl
    42. positive regulation of endothelial cell migration Source: Ensembl
    43. positive regulation of epidermal growth factor receptor signaling pathway Source: Ensembl
    44. positive regulation of extracellular matrix constituent secretion Source: RGD
    45. positive regulation of extrinsic apoptotic signaling pathway Source: Ensembl
    46. positive regulation of fatty acid biosynthetic process Source: Ensembl
    47. positive regulation of fibroblast proliferation Source: BHF-UCL
    48. positive regulation of MAPK cascade Source: Ensembl
    49. positive regulation of multicellular organism growth Source: Ensembl
    50. positive regulation of nitric oxide biosynthetic process Source: RGD
    51. positive regulation of organ growth Source: Ensembl
    52. positive regulation of phosphatidylinositol 3-kinase signaling Source: Ensembl
    53. positive regulation of protein tyrosine kinase activity Source: Ensembl
    54. positive regulation of renal sodium excretion Source: RGD
    55. positive regulation of superoxide anion generation Source: RGD
    56. positive regulation of transcription, DNA-templated Source: Ensembl
    57. positive regulation of vasodilation Source: RGD
    58. regulation of calcium ion transport Source: RGD
    59. regulation of inflammatory response Source: Ensembl
    60. regulation of long-term neuronal synaptic plasticity Source: RGD
    61. regulation of norepinephrine secretion Source: RGD
    62. regulation of proteolysis Source: RefGenome
    63. regulation of renal output by angiotensin Source: Ensembl
    64. regulation of renal sodium excretion Source: RGD
    65. regulation of transmission of nerve impulse Source: RGD
    66. renal response to blood flow involved in circulatory renin-angiotensin regulation of systemic arterial blood pressure Source: Ensembl
    67. renin-angiotensin regulation of aldosterone production Source: RGD
    68. response to cold Source: Ensembl
    69. response to muscle activity involved in regulation of muscle adaptation Source: BHF-UCL
    70. response to salt stress Source: Ensembl
    71. smooth muscle cell differentiation Source: Ensembl
    72. smooth muscle cell proliferation Source: RGD
    73. stress-activated MAPK cascade Source: RGD
    74. uterine smooth muscle contraction Source: RGD
    75. vasoconstriction Source: RGD
    76. vasodilation Source: RGD

    Keywords - Molecular functioni

    Vasoactive, Vasoconstrictor

    Enzyme and pathway databases

    ReactomeiREACT_196408. PPARA activates gene expression.
    REACT_198807. Metabolism of Angiotensinogen to Angiotensins.

    Protein family/group databases

    MEROPSiI04.953.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Angiotensinogen
    Alternative name(s):
    Serpin A8
    Cleaved into the following 8 chains:
    Alternative name(s):
    Angiotensin 1-10
    Angiotensin I
    Short name:
    Ang I
    Alternative name(s):
    Angiotensin 1-8
    Angiotensin II
    Short name:
    Ang II
    Alternative name(s):
    Angiotensin 2-8
    Angiotensin III
    Short name:
    Ang III
    Des-Asp[1]-angiotensin II
    Alternative name(s):
    Angiotensin 3-8
    Angiotensin IV
    Short name:
    Ang IV
    Gene namesi
    Name:Agt
    Synonyms:Serpina8
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 19

    Organism-specific databases

    RGDi2069. Agt.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. extracellular space Source: BHF-UCL

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 24241 PublicationAdd
    BLAST
    Chaini25 – 477453AngiotensinogenPRO_0000032468Add
    BLAST
    Peptidei25 – 3410Angiotensin-1PRO_0000032469
    Peptidei25 – 339Angiotensin 1-9PRO_0000420674
    Peptidei25 – 328Angiotensin-2PRO_0000032470
    Peptidei25 – 317Angiotensin 1-7PRO_0000420675
    Peptidei25 – 295Angiotensin 1-5PRO_0000420676
    Peptidei25 – 284Angiotensin 1-4PRO_0000420677
    Peptidei26 – 327Angiotensin-3PRO_0000032471
    Peptidei27 – 326Angiotensin-4PRO_0000420678

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi42 ↔ 1611 Publication
    Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi319 – 3191N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    In response to low blood pressure, the enzyme renin/REN cleaves angiotensinogen to produce angiotensin-1. Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4 By similarity. Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2 and can be further processed by ACE to produce angiotensin 1-7, angiotensin 1-5 and angiotensin 1-4. Angiotensin 1-7 has also been proposed to be cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin) By similarity.By similarity
    The disulfide bond is labile. Angiotensinogen is present in the circulation in a near 40:60 ratio with the oxidized disulfide-bonded form, which preferentially interacts with receptor-bound renin.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP01015.
    PRIDEiP01015.

    PTM databases

    PhosphoSiteiP01015.

    Expressioni

    Tissue specificityi

    Expressed by the liver and secreted in plasma.

    Gene expression databases

    GenevestigatoriP01015.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-1522474.
    STRINGi10116.ENSRNOP00000024917.

    Structurei

    Secondary structure

    1
    477
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni33 – 353
    Helixi39 – 435
    Helixi72 – 8413
    Helixi88 – 11528
    Beta strandi120 – 1245
    Helixi126 – 13813
    Helixi142 – 15110
    Helixi167 – 18115
    Beta strandi193 – 20311
    Helixi211 – 2166
    Turni217 – 2204
    Beta strandi224 – 2285
    Beta strandi231 – 2333
    Helixi235 – 25016
    Beta strandi267 – 27913
    Beta strandi282 – 2843
    Beta strandi289 – 2946
    Beta strandi297 – 3015
    Beta strandi303 – 31513
    Turni316 – 3194
    Beta strandi320 – 34021
    Helixi341 – 3433
    Helixi344 – 3518
    Beta strandi353 – 3553
    Beta strandi366 – 3738
    Beta strandi375 – 3828
    Helixi383 – 3897
    Turni393 – 3964
    Helixi403 – 4053
    Beta strandi406 – 4083
    Beta strandi416 – 42510
    Beta strandi452 – 4587
    Turni459 – 4624
    Beta strandi463 – 4719

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1SMRX-ray2.00B/D/F/H30-33[»]
    2WXZX-ray2.80A/C25-477[»]
    2WY1X-ray3.15A/B25-477[»]
    ProteinModelPortaliP01015.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP01015.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the serpin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG4826.
    GeneTreeiENSGT00750000117773.
    HOGENOMiHOG000033941.
    HOVERGENiHBG004233.
    InParanoidiP01015.
    KOiK09821.
    OMAiRFMQAVT.
    OrthoDBiEOG7QK0BN.
    PhylomeDBiP01015.
    TreeFamiTF343201.

    Family and domain databases

    InterProiIPR000227. Angiotensinogen.
    IPR023796. Serpin_dom.
    IPR000215. Serpin_fam.
    [Graphical view]
    PANTHERiPTHR11461. PTHR11461. 1 hit.
    PfamiPF00079. Serpin. 1 hit.
    [Graphical view]
    PRINTSiPR00654. ANGIOTENSNGN.
    SMARTiSM00093. SERPIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF56574. SSF56574. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P01015-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTPTGAGLKA TIFCILTWVS LTAGDRVYIH PFHLLYYSKS TCAQLENPSV    50
    ETLPEPTFEP VPIQAKTSPV DEKTLRDKLV LATEKLEAED RQRAAQVAMI 100
    ANFMGFRMYK MLSEARGVAS GAVLSPPALF GTLVSFYLGS LDPTASQLQV 150
    LLGVPVKEGD CTSRLDGHKV LTALQAVQGL LVTQGGSSSQ TPLLQSTVVG 200
    LFTAPGLRLK QPFVESLGPF TPAIFPRSLD LSTDPVLAAQ KINRFVQAVT 250
    GWKMNLPLEG VSTDSTLFFN TYVHFQGKMR GFSQLTGLHE FWVDNSTSVS 300
    VPMLSGTGNF QHWSDAQNNF SVTRVPLGES VTLLLIQPQC ASDLDRVEVL 350
    VFQHDFLTWI KNPPPRAIRL TLPQLEIRGS YNLQDLLAQA KLSTLLGAEA 400
    NLGKMGDTNP RVGEVLNSIL LELQAGEEEQ PTESAQQPGS PEVLDVTLSS 450
    PFLFAIYERD SGALHFLGRV DNPQNVV 477
    Length:477
    Mass (Da):51,982
    Last modified:July 21, 1986 - v1
    Checksum:i689051A5788D693D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L00094
    , L00091, L00092, L00093 Genomic DNA. Translation: AAA98779.1.
    BC078741 mRNA. Translation: AAH78741.1.
    BC087679 mRNA. Translation: AAH87679.1.
    PIRiA93945. ANRT.
    RefSeqiNP_602308.1. NM_134432.2.
    XP_006255858.1. XM_006255796.1.
    UniGeneiRn.6319.

    Genome annotation databases

    EnsembliENSRNOT00000024917; ENSRNOP00000024917; ENSRNOG00000018445.
    GeneIDi24179.
    KEGGirno:24179.
    UCSCiRGD:2069. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L00094
    , L00091 , L00092 , L00093 Genomic DNA. Translation: AAA98779.1 .
    BC078741 mRNA. Translation: AAH78741.1 .
    BC087679 mRNA. Translation: AAH87679.1 .
    PIRi A93945. ANRT.
    RefSeqi NP_602308.1. NM_134432.2.
    XP_006255858.1. XM_006255796.1.
    UniGenei Rn.6319.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1SMR X-ray 2.00 B/D/F/H 30-33 [» ]
    2WXZ X-ray 2.80 A/C 25-477 [» ]
    2WY1 X-ray 3.15 A/B 25-477 [» ]
    ProteinModelPortali P01015.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1522474.
    STRINGi 10116.ENSRNOP00000024917.

    Chemistry

    BindingDBi P01015.

    Protein family/group databases

    MEROPSi I04.953.

    PTM databases

    PhosphoSitei P01015.

    Proteomic databases

    PaxDbi P01015.
    PRIDEi P01015.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000024917 ; ENSRNOP00000024917 ; ENSRNOG00000018445 .
    GeneIDi 24179.
    KEGGi rno:24179.
    UCSCi RGD:2069. rat.

    Organism-specific databases

    CTDi 183.
    RGDi 2069. Agt.

    Phylogenomic databases

    eggNOGi COG4826.
    GeneTreei ENSGT00750000117773.
    HOGENOMi HOG000033941.
    HOVERGENi HBG004233.
    InParanoidi P01015.
    KOi K09821.
    OMAi RFMQAVT.
    OrthoDBi EOG7QK0BN.
    PhylomeDBi P01015.
    TreeFami TF343201.

    Enzyme and pathway databases

    Reactomei REACT_196408. PPARA activates gene expression.
    REACT_198807. Metabolism of Angiotensinogen to Angiotensins.

    Miscellaneous databases

    EvolutionaryTracei P01015.
    NextBioi 602521.
    PROi P01015.

    Gene expression databases

    Genevestigatori P01015.

    Family and domain databases

    InterProi IPR000227. Angiotensinogen.
    IPR023796. Serpin_dom.
    IPR000215. Serpin_fam.
    [Graphical view ]
    PANTHERi PTHR11461. PTHR11461. 1 hit.
    Pfami PF00079. Serpin. 1 hit.
    [Graphical view ]
    PRINTSi PR00654. ANGIOTENSNGN.
    SMARTi SM00093. SERPIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56574. SSF56574. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
    2. "Common structural organization of the angiotensinogen and the alpha 1-antitrypsin genes."
      Tanaka T., Ohkubo H., Nakanishi S.
      J. Biol. Chem. 259:8063-8065(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain and Kidney.
    4. "Comparative studies on angiotensins. II. Structure of rat angiotensin and its identification by DNS-method."
      Nakayama T., Nakajima T., Sokabe H.
      Chem. Pharm. Bull. 20:1579-1581(1972) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-34.
    5. "The antithrombotic effect of angiotensin-(1-7) involves mas-mediated NO release from platelets."
      Fraga-Silva R.A., Pinheiro S.V.B., Goncalves A.C., Alenina N., Bader M., Santos R.A.S.
      Mol. Med. 14:28-35(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF ANGIOTENSIN 1-7.
    6. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 25-477, DISULFIDE BOND.

    Entry informationi

    Entry nameiANGT_RAT
    AccessioniPrimary (citable) accession number: P01015
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3