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P01015 (ANGT_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Angiotensinogen
Alternative name(s):
Serpin A8

Cleaved into the following 8 chains:

  1. Angiotensin-1
    Alternative name(s):
    Angiotensin 1-10
    Angiotensin I
    Short name=Ang I
  2. Angiotensin-2
    Alternative name(s):
    Angiotensin 1-8
    Angiotensin II
    Short name=Ang II
  3. Angiotensin-3
    Alternative name(s):
    Angiotensin 2-8
    Angiotensin III
    Short name=Ang III
    Des-Asp[1]-angiotensin II
  4. Angiotensin-4
    Alternative name(s):
    Angiotensin 3-8
    Angiotensin IV
    Short name=Ang IV
  5. Angiotensin 1-9
  6. Angiotensin 1-7
  7. Angiotensin 1-5
  8. Angiotensin 1-4
Gene names
Name:Agt
Synonyms:Serpina8
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length477 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis By similarity. Ref.5

Angiotensin-2: acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone By similarity. Ref.5

Angiotensin-3: stimulates aldosterone release By similarity. Ref.5

Angiotensin 1-7: is a ligand for the G-protein coupled receptor MAS1 By similarity. Has vasodilator and antidiuretic effects By similarity. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets. Ref.5

Subcellular location

Secreted.

Tissue specificity

Expressed by the liver and secreted in plasma.

Post-translational modification

In response to low blood pressure, the enzyme renin/REN cleaves angiotensinogen to produce angiotensin-1. Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4 By similarity. Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2 and can be further processed by ACE to produce angiotensin 1-7, angiotensin 1-5 and angiotensin 1-4. Angiotensin 1-7 has also been proposed to be cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin) By similarity.

The disulfide bond is labile. Angiotensinogen is present in the circulation in a near 40:60 ratio with the oxidized disulfide-bonded form, which preferentially interacts with receptor-bound renin.

Sequence similarities

Belongs to the serpin family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionVasoactive
Vasoconstrictor
   PTMDisulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processERK1 and ERK2 cascade

Inferred from direct assay PubMed 21367774. Source: RGD

G-protein coupled receptor signaling pathway

Inferred from mutant phenotype PubMed 16286564. Source: RGD

activation of NF-kappaB-inducing kinase activity

Inferred from electronic annotation. Source: Ensembl

activation of phospholipase C activity

Inferred from mutant phenotype PubMed 11100981. Source: RGD

adrenergic receptor signaling pathway

Traceable author statement PubMed 9931144. Source: GOC

aging

Inferred from expression pattern PubMed 21318332. Source: RGD

angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure

Inferred from electronic annotation. Source: Ensembl

angiotensin-mediated drinking behavior

Inferred from direct assay PubMed 21281609. Source: RGD

artery smooth muscle contraction

Inferred from direct assay PubMed 21076237. Source: RGD

astrocyte activation

Inferred from electronic annotation. Source: Ensembl

blood vessel development

Inferred from electronic annotation. Source: Ensembl

branching involved in ureteric bud morphogenesis

Inferred from electronic annotation. Source: Ensembl

catenin import into nucleus

Inferred from direct assay PubMed 21376054. Source: RGD

cell growth involved in cardiac muscle cell development

Inferred from direct assay PubMed 21376054. Source: RGD

cell surface receptor signaling pathway

Inferred from direct assay PubMed 12130563. Source: RGD

cell-matrix adhesion

Inferred from electronic annotation. Source: Ensembl

cellular response to mechanical stimulus

Inferred from expression pattern PubMed 21131638. Source: RGD

cellular sodium ion homeostasis

Inferred from mutant phenotype PubMed 16380540. Source: RGD

cytokine secretion

Inferred from direct assay PubMed 21282555. Source: RGD

establishment of blood-nerve barrier

Inferred from electronic annotation. Source: Ensembl

excretion

Inferred from electronic annotation. Source: Ensembl

extracellular matrix organization

Inferred from electronic annotation. Source: Ensembl

fibroblast proliferation

Inferred from direct assay PubMed 21419085. Source: RGD

hormone metabolic process

Inferred from electronic annotation. Source: Ensembl

kidney development

Inferred from electronic annotation. Source: Ensembl

negative regulation of angiogenesis

Inferred from direct assay PubMed 21281454. Source: RGD

negative regulation of cell growth

Inferred from mutant phenotype PubMed 15951342. Source: RGD

negative regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

negative regulation of endopeptidase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

negative regulation of neuron apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of neurotrophin TRK receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

negative regulation of tissue remodeling

Inferred from direct assay PubMed 21167013. Source: RGD

ovarian follicle rupture

Inferred from electronic annotation. Source: Ensembl

peristalsis

Inferred from electronic annotation. Source: Ensembl

positive regulation of MAPK cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of activation of JAK2 kinase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of blood pressure

Inferred from direct assay PubMed 21318332. Source: RGD

positive regulation of branching involved in ureteric bud morphogenesis

Inferred from electronic annotation. Source: Ensembl

positive regulation of cardiac muscle hypertrophy

Inferred from direct assay PubMed 8348686. Source: BHF-UCL

positive regulation of catalytic activity

Inferred from mutant phenotype PubMed 11100981. Source: RGD

positive regulation of cholesterol esterification

Inferred from electronic annotation. Source: Ensembl

positive regulation of endothelial cell migration

Inferred from electronic annotation. Source: Ensembl

positive regulation of epidermal growth factor receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of extracellular matrix constituent secretion

Inferred from direct assay PubMed 21419085. Source: RGD

positive regulation of extrinsic apoptotic signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of fatty acid biosynthetic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of fibroblast proliferation

Inferred from direct assay PubMed 8348686. Source: BHF-UCL

positive regulation of multicellular organism growth

Inferred from electronic annotation. Source: Ensembl

positive regulation of organ growth

Inferred from electronic annotation. Source: Ensembl

positive regulation of phosphatidylinositol 3-kinase signaling

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein tyrosine kinase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of renal sodium excretion

Inferred from mutant phenotype PubMed 16380540. Source: RGD

positive regulation of superoxide anion generation

Inferred from direct assay PubMed 21376054. Source: RGD

positive regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: Ensembl

regulation of calcium ion transport

Inferred from direct assay PubMed 21423294. Source: RGD

regulation of inflammatory response

Inferred from electronic annotation. Source: Ensembl

regulation of long-term neuronal synaptic plasticity

Inferred from mutant phenotype PubMed 11282364. Source: RGD

regulation of norepinephrine secretion

Inferred from mutant phenotype PubMed 11100981. Source: RGD

regulation of proteolysis

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of renal output by angiotensin

Inferred from electronic annotation. Source: Ensembl

regulation of renal sodium excretion

Inferred from direct assay PubMed 11390024. Source: RGD

renal response to blood flow involved in circulatory renin-angiotensin regulation of systemic arterial blood pressure

Inferred from electronic annotation. Source: Ensembl

renin-angiotensin regulation of aldosterone production

Inferred from direct assay PubMed 21318332. Source: RGD

response to cold

Inferred from electronic annotation. Source: Ensembl

response to muscle activity involved in regulation of muscle adaptation

Inferred from mutant phenotype PubMed 8252633. Source: BHF-UCL

response to salt stress

Inferred from electronic annotation. Source: Ensembl

smooth muscle cell differentiation

Inferred from electronic annotation. Source: Ensembl

smooth muscle cell proliferation

Inferred from direct assay PubMed 21292788. Source: RGD

stress-activated MAPK cascade

Inferred from direct assay PubMed 21367774. Source: RGD

uterine smooth muscle contraction

Inferred from direct assay PubMed 21401609. Source: RGD

vasoconstriction

Inferred from direct assay PubMed 20955746. Source: RGD

vasodilation

Inferred from direct assay PubMed 21056585. Source: RGD

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 12242043. Source: RGD

extracellular space

Inferred from direct assay PubMed 8252633. Source: BHF-UCL

   Molecular_functionacetyltransferase activator activity

Inferred from electronic annotation. Source: Ensembl

alpha1-adrenergic receptor activity

Traceable author statement PubMed 9931144. Source: RGD

angiotensin receptor binding

Inferred from mutant phenotype PubMed 11282364PubMed 15951342. Source: RGD

hormone activity

Inferred from direct assay PubMed 8348686. Source: BHF-UCL

serine-type endopeptidase inhibitor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

type 1 angiotensin receptor binding

Inferred from mutant phenotype PubMed 16286564. Source: RGD

type 2 angiotensin receptor binding

Inferred from mutant phenotype PubMed 16286564. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Ref.4
Chain25 – 477453Angiotensinogen
PRO_0000032468
Peptide25 – 3410Angiotensin-1 Ref.4
PRO_0000032469
Peptide25 – 339Angiotensin 1-9
PRO_0000420674
Peptide25 – 328Angiotensin-2
PRO_0000032470
Peptide25 – 317Angiotensin 1-7
PRO_0000420675
Peptide25 – 295Angiotensin 1-5
PRO_0000420676
Peptide25 – 284Angiotensin 1-4
PRO_0000420677
Peptide26 – 327Angiotensin-3
PRO_0000032471
Peptide27 – 326Angiotensin-4
PRO_0000420678

Amino acid modifications

Glycosylation2951N-linked (GlcNAc...) Potential
Glycosylation3191N-linked (GlcNAc...) Potential
Disulfide bond42 ↔ 161 Ref.6

Secondary structure

............................................................ 477
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P01015 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 689051A5788D693D

FASTA47751,982
        10         20         30         40         50         60 
MTPTGAGLKA TIFCILTWVS LTAGDRVYIH PFHLLYYSKS TCAQLENPSV ETLPEPTFEP 

        70         80         90        100        110        120 
VPIQAKTSPV DEKTLRDKLV LATEKLEAED RQRAAQVAMI ANFMGFRMYK MLSEARGVAS 

       130        140        150        160        170        180 
GAVLSPPALF GTLVSFYLGS LDPTASQLQV LLGVPVKEGD CTSRLDGHKV LTALQAVQGL 

       190        200        210        220        230        240 
LVTQGGSSSQ TPLLQSTVVG LFTAPGLRLK QPFVESLGPF TPAIFPRSLD LSTDPVLAAQ 

       250        260        270        280        290        300 
KINRFVQAVT GWKMNLPLEG VSTDSTLFFN TYVHFQGKMR GFSQLTGLHE FWVDNSTSVS 

       310        320        330        340        350        360 
VPMLSGTGNF QHWSDAQNNF SVTRVPLGES VTLLLIQPQC ASDLDRVEVL VFQHDFLTWI 

       370        380        390        400        410        420 
KNPPPRAIRL TLPQLEIRGS YNLQDLLAQA KLSTLLGAEA NLGKMGDTNP RVGEVLNSIL 

       430        440        450        460        470 
LELQAGEEEQ PTESAQQPGS PEVLDVTLSS PFLFAIYERD SGALHFLGRV DNPQNVV 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequence analysis of cDNA for rat angiotensinogen."
Ohkubo H., Kageyama R., Ujihara M., Hirose T., Inayama S., Nakanishi S.
Proc. Natl. Acad. Sci. U.S.A. 80:2196-2200(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
[2]"Common structural organization of the angiotensinogen and the alpha 1-antitrypsin genes."
Tanaka T., Ohkubo H., Nakanishi S.
J. Biol. Chem. 259:8063-8065(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain and Kidney.
[4]"Comparative studies on angiotensins. II. Structure of rat angiotensin and its identification by DNS-method."
Nakayama T., Nakajima T., Sokabe H.
Chem. Pharm. Bull. 20:1579-1581(1972) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-34.
[5]"The antithrombotic effect of angiotensin-(1-7) involves mas-mediated NO release from platelets."
Fraga-Silva R.A., Pinheiro S.V.B., Goncalves A.C., Alenina N., Bader M., Santos R.A.S.
Mol. Med. 14:28-35(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF ANGIOTENSIN 1-7.
[6]"A redox switch in angiotensinogen modulates angiotensin release."
Zhou A., Carrell R.W., Murphy M.P., Wei Z., Yan Y., Stanley P.L., Stein P.E., Broughton Pipkin F., Read R.J.
Nature 468:108-111(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 25-477, DISULFIDE BOND.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L00094 expand/collapse EMBL AC list , L00091, L00092, L00093 Genomic DNA. Translation: AAA98779.1.
BC078741 mRNA. Translation: AAH78741.1.
BC087679 mRNA. Translation: AAH87679.1.
PIRANRT. A93945.
RefSeqNP_602308.1. NM_134432.2.
XP_006255858.1. XM_006255796.1.
UniGeneRn.6319.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1SMRX-ray2.00B/D/F/H30-33[»]
2WXZX-ray2.80A/C25-477[»]
2WY1X-ray3.15A/B25-477[»]
ProteinModelPortalP01015.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1522474.
STRING10116.ENSRNOP00000024917.

Chemistry

BindingDBP01015.

Protein family/group databases

MEROPSI04.953.

PTM databases

PhosphoSiteP01015.

Proteomic databases

PaxDbP01015.
PRIDEP01015.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000024917; ENSRNOP00000024917; ENSRNOG00000018445.
GeneID24179.
KEGGrno:24179.
UCSCRGD:2069. rat.

Organism-specific databases

CTD183.
RGD2069. Agt.

Phylogenomic databases

eggNOGCOG4826.
GeneTreeENSGT00750000117773.
HOGENOMHOG000033941.
HOVERGENHBG004233.
InParanoidP01015.
KOK09821.
OMARFMQAVT.
OrthoDBEOG7QK0BN.
PhylomeDBP01015.
TreeFamTF343201.

Gene expression databases

GenevestigatorP01015.

Family and domain databases

InterProIPR000227. Angiotensinogen.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERPTHR11461. PTHR11461. 1 hit.
PfamPF00079. Serpin. 1 hit.
[Graphical view]
PRINTSPR00654. ANGIOTENSNGN.
SMARTSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMSSF56574. SSF56574. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP01015.
NextBio602521.
PROP01015.

Entry information

Entry nameANGT_RAT
AccessionPrimary (citable) accession number: P01015
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references