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P01006

- SSI_STRAO

UniProt

P01006 - SSI_STRAO

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Protein
Subtilisin inhibitor
Gene
ssi
Organism
Streptomyces albogriseolus
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Strong inhibitor of bacterial serine proteases such as subtilisin.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei104 – 1052Reactive bond for subtilisin

GO - Molecular functioni

  1. serine-type endopeptidase inhibitor activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI16.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Subtilisin inhibitor
Alternative name(s):
SSI type
Gene namesi
Name:ssi
OrganismiStreptomyces albogriseolus
Taxonomic identifieri1887 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Subcellular locationi

Secreted UniRule annotation

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi104 – 1041M → D, E, V, I, G or P: Decrease in inhibition. 2 Publications
Mutagenesisi104 – 1041M → K or R: Also inhibits trypsin. 2 Publications
Mutagenesisi104 – 1041M → Y or W: Also inhibits chymotrypsin. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 31311 Publication
Add
BLAST
Chaini32 – 144113Subtilisin inhibitorUniRule annotation
PRO_0000033270Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi66 ↔ 81UniRule annotation
Disulfide bondi102 ↔ 132UniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

MINTiMINT-1345749.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi42 – 5211
Turni53 – 553
Beta strandi60 – 656
Beta strandi67 – 693
Beta strandi71 – 733
Helixi77 – 8711
Helixi91 – 933
Beta strandi98 – 1036
Beta strandi109 – 1179
Beta strandi120 – 13011
Helixi131 – 1355
Beta strandi138 – 1403

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2SICX-ray1.80I38-144[»]
2TLDX-ray2.60I35-144[»]
3SICX-ray1.80I38-144[»]
3SSIX-ray2.30A32-144[»]
5SICX-ray2.20I38-144[»]
ProteinModelPortaliP01006.
SMRiP01006. Positions 37-144.

Miscellaneous databases

EvolutionaryTraceiP01006.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati33 – 375UniRule annotation
Repeati39 – 435UniRule annotation

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di3.30.350.10. 1 hit.
HAMAPiMF_00778. SSI.
InterProiIPR000691. Prot_inh_I16_SSI.
IPR020054. Prot_inh_SSI_I16_CS.
IPR023549. Subtilisin_inhibitor.
[Graphical view]
PfamiPF00720. SSI. 1 hit.
[Graphical view]
PRINTSiPR00294. SSBTLNINHBTR.
ProDomiPD004028. Prot_inh_SSI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55399. SSF55399. 1 hit.
PROSITEiPS00999. SSI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01006-1 [UniParc]FASTAAdd to Basket

« Hide

MRNTGAGPSP SVSRPPPSAA PLSGAALAAP GDAPSALYAP SALVLTVGKG    50
VSATTAAPER AVTLTCAPGP SGTHPAAGSA CADLAAVGGD LNALTRGEDV 100
MCPMVYDPVL LTVDGVWQGK RVSYERVFSN ECEMNAHGSS VFAF 144
Length:144
Mass (Da):14,312
Last modified:November 1, 1990 - v2
Checksum:iBEA57AC7FDCD8004
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00402 Genomic DNA. Translation: BAA00305.1.
M33133 Genomic DNA. Translation: AAA26821.1.
M33134 Genomic DNA. Translation: AAA26822.1.
X73049 Genomic DNA. Translation: CAA51525.1.
M54887 Genomic DNA. Translation: AAA26827.1.
PIRiJX0066. XSSMA.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00402 Genomic DNA. Translation: BAA00305.1 .
M33133 Genomic DNA. Translation: AAA26821.1 .
M33134 Genomic DNA. Translation: AAA26822.1 .
X73049 Genomic DNA. Translation: CAA51525.1 .
M54887 Genomic DNA. Translation: AAA26827.1 .
PIRi JX0066. XSSMA.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2SIC X-ray 1.80 I 38-144 [» ]
2TLD X-ray 2.60 I 35-144 [» ]
3SIC X-ray 1.80 I 38-144 [» ]
3SSI X-ray 2.30 A 32-144 [» ]
5SIC X-ray 2.20 I 38-144 [» ]
ProteinModelPortali P01006.
SMRi P01006. Positions 37-144.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1345749.

Protein family/group databases

MEROPSi I16.003.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P01006.

Family and domain databases

Gene3Di 3.30.350.10. 1 hit.
HAMAPi MF_00778. SSI.
InterProi IPR000691. Prot_inh_I16_SSI.
IPR020054. Prot_inh_SSI_I16_CS.
IPR023549. Subtilisin_inhibitor.
[Graphical view ]
Pfami PF00720. SSI. 1 hit.
[Graphical view ]
PRINTSi PR00294. SSBTLNINHBTR.
ProDomi PD004028. Prot_inh_SSI. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF55399. SSF55399. 1 hit.
PROSITEi PS00999. SSI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and nucleotide sequence determination of gene encoding Streptomyces subtilisin inhibitor (SSI)."
    Obata S., Taguchi S., Kumagai I., Miura K.
    J. Biochem. 105:367-371(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S-3253.
  2. "Partial alteration of a protein Streptomyces subtilisin inhibitor by site-directed mutagenesis."
    Miura K., Kumagai I., Obata S., Kojima S., Taguchi S.
    Proc. Jpn. Acad., B, Phys. Biol. Sci. 64:147-149(1988)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Analysis of transcriptional control regions in the Streptomyces subtilisin-inhibitor-encoding gene."
    Taguchi S., Nishiyama K., Kumagai I., Miura K.
    Gene 84:279-286(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 32-144.
    Strain: S-3253.
  4. "Effect of a rare leucine codon, TTA, on expression of a foreign gene in Streptomyces lividans."
    Ueda Y., Taguchi S., Nishiyama K., Kumagai I., Miura K.
    Biochim. Biophys. Acta 1172:262-266(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 32-144.
  5. "Amino acid sequence of an alkaline proteinase inhibitor (Streptomyces subtilisin inhibitor) from Streptomyces albogriseolus S-3253."
    Ikenaka T., Odani S., Sakai M., Nabeshima Y., Sato S., Murao S.
    J. Biochem. 76:1191-1209(1974) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 32-144.
    Strain: S-3253.
  6. "Importance of the carboxyl-terminal four amino acid residues in the inhibitory activity of Streptomyces subtilisin inhibitor (with a revision of its carboxyl-terminal sequence)."
    Sakai M., Odani S., Ikenaka T.
    J. Biochem. 87:891-898(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 142-143.
  7. "Crystal structures of Streptomyces subtilisin inhibitor and its complex with subtilisin BPN'."
    Mitsui Y., Satow Y., Watanabe Y., Hirono S., Iitaka Y.
    Nature 277:447-452(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
  8. "Crystal structure at 2.6-A resolution of the complex of subtilisin BPN' with streptomyces subtilisin inhibitor."
    Hirono S., Akagawa H., Mitsui Y., Iitaka Y.
    J. Mol. Biol. 178:389-413(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
  9. "Alteration of the specificity of the Streptomyces subtilisin inhibitor by gene engineering."
    Kojima S., Obata S., Kumagai I., Miura K.
    Biotechnology (N.Y.) 8:449-452(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF MET-104.
  10. "Inhibition of subtilisin BPN' by reaction site P1 mutants of Streptomyces subtilisin inhibitor."
    Kojima S., Nishiyama Y., Kumagai I., Miura K.
    J. Biochem. 109:377-382(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF MET-104.

Entry informationi

Entry nameiSSI_STRAO
AccessioniPrimary (citable) accession number: P01006
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1990
Last modified: April 16, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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