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Protein

Subtilisin inhibitor

Gene

ssi

Organism
Streptomyces albogriseolus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Strong inhibitor of bacterial serine proteases such as subtilisin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei104 – 105Reactive bond for subtilisin2

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI16.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Subtilisin inhibitor
Alternative name(s):
SSI type
Gene namesi
Name:ssi
OrganismiStreptomyces albogriseolus
Taxonomic identifieri1887 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi104M → D, E, V, I, G or P: Decrease in inhibition. 2 Publications1
Mutagenesisi104M → K or R: Also inhibits trypsin. 2 Publications1
Mutagenesisi104M → Y or W: Also inhibits chymotrypsin. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 311 PublicationAdd BLAST31
ChainiPRO_000003327032 – 144Subtilisin inhibitorAdd BLAST113

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi66 ↔ 81
Disulfide bondi102 ↔ 132

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

MINTiMINT-1345749.

Structurei

Secondary structure

1144
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi42 – 52Combined sources11
Turni53 – 55Combined sources3
Beta strandi60 – 65Combined sources6
Beta strandi67 – 69Combined sources3
Beta strandi71 – 73Combined sources3
Helixi77 – 87Combined sources11
Helixi91 – 93Combined sources3
Beta strandi98 – 103Combined sources6
Beta strandi109 – 117Combined sources9
Beta strandi120 – 130Combined sources11
Helixi131 – 135Combined sources5
Beta strandi138 – 140Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2SICX-ray1.80I38-144[»]
2TLDX-ray2.60I35-144[»]
3SICX-ray1.80I38-144[»]
3SSIX-ray2.30A32-144[»]
5SICX-ray2.20I38-144[»]
ProteinModelPortaliP01006.
SMRiP01006.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01006.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati33 – 375
Repeati39 – 435

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di3.30.350.10. 1 hit.
HAMAPiMF_00778. SSI. 1 hit.
InterProiIPR000691. Prot_inh_I16_SSI.
IPR020054. Prot_inh_SSI_I16_CS.
IPR023549. Subtilisin_inhibitor.
[Graphical view]
PfamiPF00720. SSI. 1 hit.
[Graphical view]
PRINTSiPR00294. SSBTLNINHBTR.
ProDomiPD004028. Prot_inh_SSI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55399. SSF55399. 1 hit.
PROSITEiPS00999. SSI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01006-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNTGAGPSP SVSRPPPSAA PLSGAALAAP GDAPSALYAP SALVLTVGKG
60 70 80 90 100
VSATTAAPER AVTLTCAPGP SGTHPAAGSA CADLAAVGGD LNALTRGEDV
110 120 130 140
MCPMVYDPVL LTVDGVWQGK RVSYERVFSN ECEMNAHGSS VFAF
Length:144
Mass (Da):14,312
Last modified:November 1, 1990 - v2
Checksum:iBEA57AC7FDCD8004
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00402 Genomic DNA. Translation: BAA00305.1.
M33133 Genomic DNA. Translation: AAA26821.1.
M33134 Genomic DNA. Translation: AAA26822.1.
X73049 Genomic DNA. Translation: CAA51525.1.
M54887 Genomic DNA. Translation: AAA26827.1.
PIRiJX0066. XSSMA.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00402 Genomic DNA. Translation: BAA00305.1.
M33133 Genomic DNA. Translation: AAA26821.1.
M33134 Genomic DNA. Translation: AAA26822.1.
X73049 Genomic DNA. Translation: CAA51525.1.
M54887 Genomic DNA. Translation: AAA26827.1.
PIRiJX0066. XSSMA.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2SICX-ray1.80I38-144[»]
2TLDX-ray2.60I35-144[»]
3SICX-ray1.80I38-144[»]
3SSIX-ray2.30A32-144[»]
5SICX-ray2.20I38-144[»]
ProteinModelPortaliP01006.
SMRiP01006.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1345749.

Protein family/group databases

MEROPSiI16.003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP01006.

Family and domain databases

Gene3Di3.30.350.10. 1 hit.
HAMAPiMF_00778. SSI. 1 hit.
InterProiIPR000691. Prot_inh_I16_SSI.
IPR020054. Prot_inh_SSI_I16_CS.
IPR023549. Subtilisin_inhibitor.
[Graphical view]
PfamiPF00720. SSI. 1 hit.
[Graphical view]
PRINTSiPR00294. SSBTLNINHBTR.
ProDomiPD004028. Prot_inh_SSI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55399. SSF55399. 1 hit.
PROSITEiPS00999. SSI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSSI_STRAO
AccessioniPrimary (citable) accession number: P01006
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1990
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.