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P01006

- SSI_STRAO

UniProt

P01006 - SSI_STRAO

Protein

Subtilisin inhibitor

Gene

ssi

Organism
Streptomyces albogriseolus
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Strong inhibitor of bacterial serine proteases such as subtilisin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei104 – 1052Reactive bond for subtilisin

    GO - Molecular functioni

    1. serine-type endopeptidase inhibitor activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Protease inhibitor, Serine protease inhibitor

    Protein family/group databases

    MEROPSiI16.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Subtilisin inhibitor
    Alternative name(s):
    SSI type
    Gene namesi
    Name:ssi
    OrganismiStreptomyces albogriseolus
    Taxonomic identifieri1887 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi104 – 1041M → D, E, V, I, G or P: Decrease in inhibition. 2 Publications
    Mutagenesisi104 – 1041M → K or R: Also inhibits trypsin. 2 Publications
    Mutagenesisi104 – 1041M → Y or W: Also inhibits chymotrypsin. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 31311 PublicationAdd
    BLAST
    Chaini32 – 144113Subtilisin inhibitorPRO_0000033270Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi66 ↔ 81
    Disulfide bondi102 ↔ 132

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    MINTiMINT-1345749.

    Structurei

    Secondary structure

    1
    144
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi42 – 5211
    Turni53 – 553
    Beta strandi60 – 656
    Beta strandi67 – 693
    Beta strandi71 – 733
    Helixi77 – 8711
    Helixi91 – 933
    Beta strandi98 – 1036
    Beta strandi109 – 1179
    Beta strandi120 – 13011
    Helixi131 – 1355
    Beta strandi138 – 1403

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2SICX-ray1.80I38-144[»]
    2TLDX-ray2.60I35-144[»]
    3SICX-ray1.80I38-144[»]
    3SSIX-ray2.30A32-144[»]
    5SICX-ray2.20I38-144[»]
    ProteinModelPortaliP01006.
    SMRiP01006. Positions 37-144.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP01006.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati33 – 375
    Repeati39 – 435

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di3.30.350.10. 1 hit.
    HAMAPiMF_00778. SSI.
    InterProiIPR000691. Prot_inh_I16_SSI.
    IPR020054. Prot_inh_SSI_I16_CS.
    IPR023549. Subtilisin_inhibitor.
    [Graphical view]
    PfamiPF00720. SSI. 1 hit.
    [Graphical view]
    PRINTSiPR00294. SSBTLNINHBTR.
    ProDomiPD004028. Prot_inh_SSI. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF55399. SSF55399. 1 hit.
    PROSITEiPS00999. SSI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P01006-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRNTGAGPSP SVSRPPPSAA PLSGAALAAP GDAPSALYAP SALVLTVGKG    50
    VSATTAAPER AVTLTCAPGP SGTHPAAGSA CADLAAVGGD LNALTRGEDV 100
    MCPMVYDPVL LTVDGVWQGK RVSYERVFSN ECEMNAHGSS VFAF 144
    Length:144
    Mass (Da):14,312
    Last modified:November 1, 1990 - v2
    Checksum:iBEA57AC7FDCD8004
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00402 Genomic DNA. Translation: BAA00305.1.
    M33133 Genomic DNA. Translation: AAA26821.1.
    M33134 Genomic DNA. Translation: AAA26822.1.
    X73049 Genomic DNA. Translation: CAA51525.1.
    M54887 Genomic DNA. Translation: AAA26827.1.
    PIRiJX0066. XSSMA.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00402 Genomic DNA. Translation: BAA00305.1 .
    M33133 Genomic DNA. Translation: AAA26821.1 .
    M33134 Genomic DNA. Translation: AAA26822.1 .
    X73049 Genomic DNA. Translation: CAA51525.1 .
    M54887 Genomic DNA. Translation: AAA26827.1 .
    PIRi JX0066. XSSMA.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2SIC X-ray 1.80 I 38-144 [» ]
    2TLD X-ray 2.60 I 35-144 [» ]
    3SIC X-ray 1.80 I 38-144 [» ]
    3SSI X-ray 2.30 A 32-144 [» ]
    5SIC X-ray 2.20 I 38-144 [» ]
    ProteinModelPortali P01006.
    SMRi P01006. Positions 37-144.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1345749.

    Protein family/group databases

    MEROPSi I16.003.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P01006.

    Family and domain databases

    Gene3Di 3.30.350.10. 1 hit.
    HAMAPi MF_00778. SSI.
    InterProi IPR000691. Prot_inh_I16_SSI.
    IPR020054. Prot_inh_SSI_I16_CS.
    IPR023549. Subtilisin_inhibitor.
    [Graphical view ]
    Pfami PF00720. SSI. 1 hit.
    [Graphical view ]
    PRINTSi PR00294. SSBTLNINHBTR.
    ProDomi PD004028. Prot_inh_SSI. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF55399. SSF55399. 1 hit.
    PROSITEi PS00999. SSI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and nucleotide sequence determination of gene encoding Streptomyces subtilisin inhibitor (SSI)."
      Obata S., Taguchi S., Kumagai I., Miura K.
      J. Biochem. 105:367-371(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S-3253.
    2. "Partial alteration of a protein Streptomyces subtilisin inhibitor by site-directed mutagenesis."
      Miura K., Kumagai I., Obata S., Kojima S., Taguchi S.
      Proc. Jpn. Acad., B, Phys. Biol. Sci. 64:147-149(1988)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Analysis of transcriptional control regions in the Streptomyces subtilisin-inhibitor-encoding gene."
      Taguchi S., Nishiyama K., Kumagai I., Miura K.
      Gene 84:279-286(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 32-144.
      Strain: S-3253.
    4. "Effect of a rare leucine codon, TTA, on expression of a foreign gene in Streptomyces lividans."
      Ueda Y., Taguchi S., Nishiyama K., Kumagai I., Miura K.
      Biochim. Biophys. Acta 1172:262-266(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 32-144.
    5. "Amino acid sequence of an alkaline proteinase inhibitor (Streptomyces subtilisin inhibitor) from Streptomyces albogriseolus S-3253."
      Ikenaka T., Odani S., Sakai M., Nabeshima Y., Sato S., Murao S.
      J. Biochem. 76:1191-1209(1974) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 32-144.
      Strain: S-3253.
    6. "Importance of the carboxyl-terminal four amino acid residues in the inhibitory activity of Streptomyces subtilisin inhibitor (with a revision of its carboxyl-terminal sequence)."
      Sakai M., Odani S., Ikenaka T.
      J. Biochem. 87:891-898(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO 142-143.
    7. "Crystal structures of Streptomyces subtilisin inhibitor and its complex with subtilisin BPN'."
      Mitsui Y., Satow Y., Watanabe Y., Hirono S., Iitaka Y.
      Nature 277:447-452(1979) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
    8. "Crystal structure at 2.6-A resolution of the complex of subtilisin BPN' with streptomyces subtilisin inhibitor."
      Hirono S., Akagawa H., Mitsui Y., Iitaka Y.
      J. Mol. Biol. 178:389-413(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
    9. "Alteration of the specificity of the Streptomyces subtilisin inhibitor by gene engineering."
      Kojima S., Obata S., Kumagai I., Miura K.
      Biotechnology (N.Y.) 8:449-452(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF MET-104.
    10. "Inhibition of subtilisin BPN' by reaction site P1 mutants of Streptomyces subtilisin inhibitor."
      Kojima S., Nishiyama Y., Kumagai I., Miura K.
      J. Biochem. 109:377-382(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF MET-104.

    Entry informationi

    Entry nameiSSI_STRAO
    AccessioniPrimary (citable) accession number: P01006
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 98 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3