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Protein

Double-headed protease inhibitor, submandibular gland

Gene
N/A
Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

This inhibitor is composed of two homologous actively inhibiting halves: one which inhibits trypsin, the other which inhibits elastase.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei26 – 272Reactive bond 1 for trypsin
Sitei77 – 782Reactive bond 2 for elastase

GO - Molecular functioni

  1. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI01.016.

Names & Taxonomyi

Protein namesi
Recommended name:
Double-headed protease inhibitor, submandibular gland
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 115115Double-headed protease inhibitor, submandibular glandPRO_0000073035Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi12 ↔ 46PROSITE-ProRule annotation
Disulfide bondi24 ↔ 43PROSITE-ProRule annotation
Disulfide bondi32 ↔ 64PROSITE-ProRule annotation
Disulfide bondi68 ↔ 97PROSITE-ProRule annotation
Disulfide bondi75 ↔ 94PROSITE-ProRule annotation
Disulfide bondi83 ↔ 115PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000009968.

Structurei

3D structure databases

ProteinModelPortaliP01002.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 6661Kazal-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini67 – 11549Kazal-like 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 Kazal-like domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG265458.
HOGENOMiHOG000202775.
HOVERGENiHBG006172.

Family and domain databases

InterProiIPR002350. Kazal_dom.
IPR001239. Prot_inh_Kazal-m.
[Graphical view]
PfamiPF00050. Kazal_1. 2 hits.
[Graphical view]
PRINTSiPR00290. KAZALINHBTR.
SMARTiSM00280. KAZAL. 2 hits.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 2 hits.
PS51465. KAZAL_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P01002-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
GPPPAIGREV DCSNYKGKGS QIACPRLHQP ICGTDHKTYS NECMFCALTL
60 70 80 90 100
NKKFEVRKLQ DTACDIECTE YSDMCTMDYR PLCGSDGKNY SNKCSFCNAV
110
KKSRGTIFLA KHGEC
Length:115
Mass (Da):12,775
Last modified:August 1, 1988 - v1
Checksum:i1F901F163EE2A35C
GO

Sequence databases

PIRiC29654. TIDGS.

Cross-referencesi

Sequence databases

PIRiC29654. TIDGS.

3D structure databases

ProteinModelPortaliP01002.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000009968.

Protein family/group databases

MEROPSiI01.016.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiNOG265458.
HOGENOMiHOG000202775.
HOVERGENiHBG006172.

Family and domain databases

InterProiIPR002350. Kazal_dom.
IPR001239. Prot_inh_Kazal-m.
[Graphical view]
PfamiPF00050. Kazal_1. 2 hits.
[Graphical view]
PRINTSiPR00290. KAZALINHBTR.
SMARTiSM00280. KAZAL. 2 hits.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 2 hits.
PS51465. KAZAL_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The amino-acid sequences of the double-headed proteinase inhibitors from cat, lion and dog submandibular glands."
    Reisinger P.W.M., Hochstrasser K., Gottlicher I., Eulitz M., Wachter E.
    Biol. Chem. Hoppe-Seyler 368:717-726(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Tissue: Submandibular gland.
  2. "The amino acid sequence of the double-headed proteinase inhibitor from canine submandibular glands, III. Sequencing studies."
    Hochstrasser K., Bretzel G., Wachter E., Heindl S.
    Hoppe-Seyler's Z. Physiol. Chem. 356:1865-1877(1975) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PROTEIN SEQUENCE.
    Tissue: Submandibular gland.

Entry informationi

Entry nameiIPSG_CANFA
AccessioniPrimary (citable) accession number: P01002
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1988
Last modified: January 7, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Three very similar inhibitors found in the submandibular glands are secreted into the saliva. The sequence shown is one of the two main inhibitors.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.