P01002 (IPSG_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Double-headed protease inhibitor, submandibular gland
Organism	Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]
Taxonomic identifier	9615 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › Canis lupus

Protein attributes

Sequence length	115 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This inhibitor is composed of two homologous actively inhibiting halves: one which inhibits trypsin, the other which inhibits elastase.
Subcellular location	Secreted.
Miscellaneous	Three very similar inhibitors found in the submandibular glands are secreted into the saliva. The sequence shown is one of the two main inhibitors.
Sequence similarities	Contains 2 Kazal-like domains.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 115	115	Double-headed protease inhibitor, submandibular gland		PRO_0000073035
Regions
	Domain	6 – 66	61	Kazal-like 1
	Domain	67 – 115	49	Kazal-like 2
Sites
	Site	26 – 27	2	Reactive bond 1 for trypsin
	Site	77 – 78	2	Reactive bond 2 for elastase
Amino acid modifications
	Disulfide bond	12 ↔ 46		By similarity
	Disulfide bond	24 ↔ 43		By similarity
	Disulfide bond	32 ↔ 64		By similarity
	Disulfide bond	68 ↔ 97		By similarity
	Disulfide bond	75 ↔ 94		By similarity
	Disulfide bond	83 ↔ 115		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P01002 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 1F901F163EE2A35C	FASTA	115	12,775
	10 20 30 40 50 60 GPPPAIGREV DCSNYKGKGS QIACPRLHQP ICGTDHKTYS NECMFCALTL NKKFEVRKLQ 70 80 90 100 110 DTACDIECTE YSDMCTMDYR PLCGSDGKNY SNKCSFCNAV KKSRGTIFLA KHGEC

References

[1]	"The amino-acid sequences of the double-headed proteinase inhibitors from cat, lion and dog submandibular glands." Reisinger P.W.M., Hochstrasser K., Gottlicher I., Eulitz M., Wachter E. Biol. Chem. Hoppe-Seyler 368:717-726(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Submandibular gland.
[2]	"The amino acid sequence of the double-headed proteinase inhibitor from canine submandibular glands, III. Sequencing studies." Hochstrasser K., Bretzel G., Wachter E., Heindl S. Hoppe-Seyler's Z. Physiol. Chem. 356:1865-1877(1975) [PubMed] [Europe PMC] [Abstract] Cited for: PRELIMINARY PROTEIN SEQUENCE. Tissue: Submandibular gland.

Cross-references

Sequence databases
PIR	TIDGS. C29654.
3D structure databases
ProteinModelPortal	P01002.
ModBase	Search...
Protein family/group databases
MEROPS	I01.016.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG265458.
HOGENOM	HOG000202775.
HOVERGEN	HBG006172.
InParanoid	P01002.
OrthoDB	EOG4K6G5H.
Family and domain databases
InterPro	IPR002350. Kazal_dom. IPR001239. Prot_inh_Kazal-m. [Graphical view]
Pfam	PF00050. Kazal_1. 2 hits. [Graphical view]
PRINTS	PR00290. KAZALINHBTR.
SMART	SM00280. KAZAL. 2 hits. [Graphical view]
PROSITE	PS00282. KAZAL_1. 2 hits. PS51465. KAZAL_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPSG_CANFA

Accession

Primary (citable) accession number: P01002

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 1, 1988
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program