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P01002 (IPSG_CANFA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Double-headed protease inhibitor, submandibular gland
OrganismCanis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]
Taxonomic identifier9615 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis

Protein attributes

Sequence length115 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This inhibitor is composed of two homologous actively inhibiting halves: one which inhibits trypsin, the other which inhibits elastase.

Subcellular location

Secreted.

Miscellaneous

Three very similar inhibitors found in the submandibular glands are secreted into the saliva. The sequence shown is one of the two main inhibitors.

Sequence similarities

Contains 2 Kazal-like domains.

Ontologies

Keywords
   Cellular componentSecreted
   DomainRepeat
   Molecular functionProtease inhibitor
Serine protease inhibitor
   PTMDisulfide bond
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase inhibitor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 115115Double-headed protease inhibitor, submandibular gland
PRO_0000073035

Regions

Domain6 – 6661Kazal-like 1
Domain67 – 11549Kazal-like 2

Sites

Site26 – 272Reactive bond 1 for trypsin
Site77 – 782Reactive bond 2 for elastase

Amino acid modifications

Disulfide bond12 ↔ 46 By similarity
Disulfide bond24 ↔ 43 By similarity
Disulfide bond32 ↔ 64 By similarity
Disulfide bond68 ↔ 97 By similarity
Disulfide bond75 ↔ 94 By similarity
Disulfide bond83 ↔ 115 By similarity

Sequences

Sequence LengthMass (Da)Tools
P01002 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: 1F901F163EE2A35C

FASTA11512,775
        10         20         30         40         50         60 
GPPPAIGREV DCSNYKGKGS QIACPRLHQP ICGTDHKTYS NECMFCALTL NKKFEVRKLQ 

        70         80         90        100        110 
DTACDIECTE YSDMCTMDYR PLCGSDGKNY SNKCSFCNAV KKSRGTIFLA KHGEC 

« Hide

References

[1]"The amino-acid sequences of the double-headed proteinase inhibitors from cat, lion and dog submandibular glands."
Reisinger P.W.M., Hochstrasser K., Gottlicher I., Eulitz M., Wachter E.
Biol. Chem. Hoppe-Seyler 368:717-726(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
Tissue: Submandibular gland.
[2]"The amino acid sequence of the double-headed proteinase inhibitor from canine submandibular glands, III. Sequencing studies."
Hochstrasser K., Bretzel G., Wachter E., Heindl S.
Hoppe-Seyler's Z. Physiol. Chem. 356:1865-1877(1975) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY PROTEIN SEQUENCE.
Tissue: Submandibular gland.

Cross-references

Sequence databases

PIRTIDGS. C29654.

3D structure databases

ProteinModelPortalP01002.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9615.ENSCAFP00000009968.

Protein family/group databases

MEROPSI01.016.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGNOG265458.
HOGENOMHOG000202775.
HOVERGENHBG006172.
InParanoidP01002.

Family and domain databases

InterProIPR002350. Kazal_dom.
IPR001239. Prot_inh_Kazal-m.
[Graphical view]
PfamPF00050. Kazal_1. 2 hits.
[Graphical view]
PRINTSPR00290. KAZALINHBTR.
SMARTSM00280. KAZAL. 2 hits.
[Graphical view]
PROSITEPS00282. KAZAL_1. 2 hits.
PS51465. KAZAL_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIPSG_CANFA
AccessionPrimary (citable) accession number: P01002
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1988
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families