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Protein

Serine protease inhibitor Kazal-type 6

Gene

SPINK6

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease inhibitor selective for kallikreins. Efficiently inhibits KLK4, KLK5, KLK6, KLK7, KLK12, KLK13 and KLK14. Doesn't inhibit KLK8. Inhibits acrosin, trypsin, and chymotrypsin.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei42 – 432Reactive bond

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI01.015.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease inhibitor Kazal-type 6
Alternative name(s):
Acrosin inhibitor 2
Acrosin inhibitor IIA
BUSI-II
Gene namesi
Name:SPINK6
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 8057Serine protease inhibitor Kazal-type 6PRO_0000073033Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei24 – 241Pyrrolidone carboxylic acid
Disulfide bondi30 ↔ 62
Disulfide bondi40 ↔ 59
Disulfide bondi48 ↔ 80

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP01001.
PRIDEiP01001.

Expressioni

Tissue specificityi

Seminal plasma.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000044797.

Structurei

Secondary structure

1
80
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi31 – 333Combined sources
Turni35 – 384Combined sources
Beta strandi41 – 444Combined sources
Beta strandi47 – 526Combined sources
Beta strandi53 – 564Combined sources
Helixi58 – 669Combined sources
Beta strandi68 – 703Combined sources
Beta strandi74 – 785Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BUSNMR-A25-80[»]
2BUSNMR-A25-80[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01001.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 8057Kazal-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Kazal-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3649. Eukaryota.
ENOG410YC3T. LUCA.
GeneTreeiENSGT00530000064225.
HOGENOMiHOG000090244.
HOVERGENiHBG006182.
InParanoidiP01001.
OMAiFCFLTGV.
OrthoDBiEOG7BW0M2.

Family and domain databases

InterProiIPR002350. Kazal_dom.
[Graphical view]
PfamiPF00050. Kazal_1. 1 hit.
[Graphical view]
SMARTiSM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTSGVFLLL SLALFCFFSG VFGQGAQVDC AEFKDPKVYC TRESNPHCGS
60 70 80
DGQTYGNKCA FCKAVMKSGG KINLKHRGKC
Length:80
Mass (Da):8,663
Last modified:September 11, 2007 - v3
Checksum:i91E3215704596CD1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti51 – 533DGQ → NGE AA sequence (PubMed:500016).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti24 – 263QGA → LF in IIB.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DY218174 mRNA. No translation available.
PIRiA01235. XTBO.
RefSeqiXP_002706142.1. XM_002706096.4.
XP_015327798.1. XM_015472312.1.

Genome annotation databases

EnsembliENSBTAT00000047606; ENSBTAP00000044797; ENSBTAG00000033470.
GeneIDi100335759.
KEGGibta:100335759.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DY218174 mRNA. No translation available.
PIRiA01235. XTBO.
RefSeqiXP_002706142.1. XM_002706096.4.
XP_015327798.1. XM_015472312.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BUSNMR-A25-80[»]
2BUSNMR-A25-80[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000044797.

Protein family/group databases

MEROPSiI01.015.

Proteomic databases

PaxDbiP01001.
PRIDEiP01001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000047606; ENSBTAP00000044797; ENSBTAG00000033470.
GeneIDi100335759.
KEGGibta:100335759.

Organism-specific databases

CTDi404203.

Phylogenomic databases

eggNOGiKOG3649. Eukaryota.
ENOG410YC3T. LUCA.
GeneTreeiENSGT00530000064225.
HOGENOMiHOG000090244.
HOVERGENiHBG006182.
InParanoidiP01001.
OMAiFCFLTGV.
OrthoDBiEOG7BW0M2.

Miscellaneous databases

EvolutionaryTraceiP01001.

Family and domain databases

InterProiIPR002350. Kazal_dom.
[Graphical view]
PfamiPF00050. Kazal_1. 1 hit.
[Graphical view]
SMARTiSM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Seminal vesicle.
  2. "Differences in the evolution of seminal plasma acrosin inhibitors and pancreatic secretory trypsin inhibitors."
    Cechova D., Meloun B.
    Hoppe-Seyler's Z. Physiol. Chem. 360:1497-1500(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-80, VARIANT IIB.
  3. "Reinvestigation of the C-terminal end in the amino acid sequence of the proteinase inhibitor IIA from bull seminal plasma."
    Frank G.
    J. Mol. Biol. 166:665-668(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 79-80.
  4. "Characterization of the proteinase inhibitor IIA from bull seminal plasma by 1H nuclear magnetic resonance. Stability, amide proton exchange and mobility of aromatic residues."
    Strop P., Wuethrich K.
    J. Mol. Biol. 166:631-640(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 24-80.
  5. "Assignment of the 1H nuclear magnetic resonance spectrum of the proteinase inhibitor IIA from bull seminal plasma by two-dimensional nuclear magnetic resonance at 500 MHz."
    Strop P., Wider G., Wuethrich K.
    J. Mol. Biol. 166:641-665(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 24-80, SEQUENCE REVISION.
  6. "Preliminary structural comparison of the proteinase isoinhibitors IIA and IIB from bull seminal plasma based on individual assignments of the 1H nuclear magnetic resonance spectra by two-dimensional nuclear magnetic resonance at 500 MHz."
    Strop P., Cechova D., Wuethrich K.
    J. Mol. Biol. 166:669-676(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 24-80 OF VARIANT IIB.
  7. "Secondary structure in the solution conformation of the proteinase inhibitor IIA from bull seminal plasma by nuclear magnetic resonance."
    Williamson M.P., Marion D., Wuethrich K.
    J. Mol. Biol. 173:341-359(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 24-80.
  8. "Solution conformation of proteinase inhibitor IIA from bull seminal plasma by 1H nuclear magnetic resonance and distance geometry."
    Williamson M.P., Havel T.F., Wuethrich K.
    J. Mol. Biol. 182:295-315(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 24-80.

Entry informationi

Entry nameiISK6_BOVIN
AccessioniPrimary (citable) accession number: P01001
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: September 11, 2007
Last modified: May 11, 2016
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The sequence of BUSI-IIA is shown.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.