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Protein

Serine protease inhibitor Kazal-type 1

Gene

SPINK1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:5466061). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens (By similarity).By similarity1 Publication
In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei18 – 19Reactive bond for trypsinPROSITE-ProRule annotationBy similarity2
Sitei20 – 21Necessary for sperm bindingBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI01.011.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease inhibitor Kazal-type 1By similarity
Alternative name(s):
Pancreatic secretory trypsin inhibitor1 Publication
Gene namesi
Name:SPINK1
Synonyms:PSTI
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000730271 – 56Serine protease inhibitor Kazal-type 1Add BLAST56

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi9 ↔ 38PROSITE-ProRule annotation1 Publication
Disulfide bondi16 ↔ 35PROSITE-ProRule annotation1 Publication
Disulfide bondi24 ↔ 56PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP00998.
PeptideAtlasiP00998.
PRIDEiP00998.

Expressioni

Gene expression databases

GenevisibleiP00998. SS.

Interactioni

Protein-protein interaction databases

MINTiMINT-1505130.
STRINGi9823.ENSSSCP00000022179.

Structurei

Secondary structure

156
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 17Combined sources6
Beta strandi23 – 25Combined sources3
Beta strandi30 – 33Combined sources4
Helixi34 – 42Combined sources9
Beta strandi50 – 54Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TGSX-ray1.80I1-56[»]
ProteinModelPortaliP00998.
SMRiP00998.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00998.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 56Kazal-likePROSITE-ProRule annotationAdd BLAST54

Phylogenomic databases

eggNOGiKOG3649. Eukaryota.
ENOG410YC3T. LUCA.
GeneTreeiENSGT00530000064228.
HOVERGENiHBG006182.
InParanoidiP00998.
OMAiTKIYNPV.
OrthoDBiEOG091G14HG.

Family and domain databases

InterProiView protein in InterPro
IPR002350. Kazal_dom.
IPR001239. Prot_inh_Kazal-m.
PfamiView protein in Pfam
PF00050. Kazal_1. 1 hit.
PRINTSiPR00290. KAZALINHBTR.
SMARTiView protein in SMART
SM00280. KAZAL. 1 hit.
SUPFAMiSSF100895. SSF100895. 1 hit.
PROSITEiView protein in PROSITE
PS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P00998-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
TSPQREATCT SEVSGCPKIY NPVCGTDGIT YSNECVLCSE NKKRQTPVLI

QKSGPC
Length:56
Mass (Da):6,023
Last modified:July 21, 1986 - v1
Checksum:i39A3649DADF16D25
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti1 – 4Missing in a second inhibitor. 4

Sequence databases

PIRiA91174. TIPG.

Genome annotation databases

EnsembliENSSSCT00000026654; ENSSSCP00000022179; ENSSSCG00000027244.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiISK1_PIG
AccessioniPrimary (citable) accession number: P00998
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 7, 2017
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references