Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P00998

- ISK1_PIG

UniProt

P00998 - ISK1_PIG

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Pancreatic secretory trypsin inhibitor

Gene

SPINK1

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This is a trypsin inhibitor, its physiological function is to prevent the trypsin-catalyzed premature activation of zymogens within the pancreas.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei18 – 192Reactive bond

GO - Molecular functioni

  1. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

GO - Biological processi

  1. negative regulation of calcium ion import Source: Ensembl
  2. negative regulation of nitric oxide mediated signal transduction Source: Ensembl
  3. negative regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  4. negative regulation of serine-type endopeptidase activity Source: Ensembl
  5. regulation of acrosome reaction Source: Ensembl
  6. regulation of store-operated calcium entry Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI01.011.

Names & Taxonomyi

Protein namesi
Recommended name:
Pancreatic secretory trypsin inhibitor
Alternative name(s):
Serine protease inhibitor Kazal-type 1
Gene namesi
Name:SPINK1
Synonyms:PSTI
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 2

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5656Pancreatic secretory trypsin inhibitorPRO_0000073027Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi9 ↔ 381 PublicationPROSITE-ProRule annotation
Disulfide bondi16 ↔ 351 PublicationPROSITE-ProRule annotation
Disulfide bondi24 ↔ 561 PublicationPROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

MINTiMINT-1505130.

Structurei

Secondary structure

1
56
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi12 – 176Combined sources
Beta strandi23 – 253Combined sources
Beta strandi30 – 334Combined sources
Helixi34 – 429Combined sources
Beta strandi50 – 545Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TGSX-ray1.80I1-56[»]
ProteinModelPortaliP00998.
SMRiP00998. Positions 1-56.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00998.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 5654Kazal-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Kazal-like domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000064228.
HOVERGENiHBG006182.
InParanoidiP00998.
OMAiNKEREVP.
OrthoDBiEOG7BW0M2.

Family and domain databases

InterProiIPR002350. Kazal_dom.
IPR001239. Prot_inh_Kazal-m.
[Graphical view]
PfamiPF00050. Kazal_1. 1 hit.
[Graphical view]
PRINTSiPR00290. KAZALINHBTR.
SMARTiSM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00998-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
TSPQREATCT SEVSGCPKIY NPVCGTDGIT YSNECVLCSE NKKRQTPVLI

QKSGPC
Length:56
Mass (Da):6,023
Last modified:July 21, 1986 - v1
Checksum:i39A3649DADF16D25
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti1 – 44Missing in a second inhibitor.

Sequence databases

PIRiA91174. TIPG.

Genome annotation databases

EnsembliENSSSCT00000026654; ENSSSCP00000022179; ENSSSCG00000027244.

Cross-referencesi

Sequence databases

PIRi A91174. TIPG.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1TGS X-ray 1.80 I 1-56 [» ]
ProteinModelPortali P00998.
SMRi P00998. Positions 1-56.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1505130.

Protein family/group databases

MEROPSi I01.011.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSSSCT00000026654 ; ENSSSCP00000022179 ; ENSSSCG00000027244 .

Phylogenomic databases

GeneTreei ENSGT00530000064228.
HOVERGENi HBG006182.
InParanoidi P00998.
OMAi NKEREVP.
OrthoDBi EOG7BW0M2.

Miscellaneous databases

EvolutionaryTracei P00998.

Family and domain databases

InterProi IPR002350. Kazal_dom.
IPR001239. Prot_inh_Kazal-m.
[Graphical view ]
Pfami PF00050. Kazal_1. 1 hit.
[Graphical view ]
PRINTSi PR00290. KAZALINHBTR.
SMARTi SM00280. KAZAL. 1 hit.
[Graphical view ]
PROSITEi PS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The structure of the porcine pancreatic secretory trypsin inhibitor. I. A sequence determination by Edman degradation and mass spectral identification of the p-bromophenyl-thiohydantoins."
    Tschesche H., Wachter E.
    Eur. J. Biochem. 16:187-198(1970) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  2. "The primary structure of the porcine pancreatic secretory trypsin inhibitor. I. Amino acid sequence of the reduced S-aminoethylated protein."
    Bartelt D.C., Greene L.J.
    J. Biol. Chem. 246:2218-2229(1971) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  3. "Disulfide bridges of the secretory trypsin inhibitor from procine pancreas and the degradation of covalent structure during the temporary inhibition."
    Tschesche H., Schneider M., Reidel G., Klein H.
    Hoppe-Seyler's Z. Physiol. Chem. 353:763-764(1972) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.
  4. "Trypsin inhibitors. VII. Primary structure of the specific trypsin inhibitor II (Kazal-type) from porcine pancreas. Sequence analysis with mass spectrometry identification of the p-bromophenylthio-hydantoins from the Edman degradation."
    Tschesche H., Wachter E.
    Hoppe-Seyler's Z. Physiol. Chem. 351:1449-1459(1970) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF A SECOND INHIBITOR.
  5. "Three-dimensional structure of the complex between pancreatic secretory trypsin inhibitor (Kazal type) and trypsinogen at 1.8-A resolution. Structure solution, crystallographic refinement and preliminary structural interpretation."
    Bolognesi M., Gatti G., Menegatti E., Guarneri M., Marquart M., Papamokos E., Huber R.
    J. Mol. Biol. 162:839-868(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).

Entry informationi

Entry nameiISK1_PIG
AccessioniPrimary (citable) accession number: P00998
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 26, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3