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Protein

Serine protease inhibitor Kazal-type 1

Gene

SPINK1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:5466061). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens (By similarity).By similarity1 Publication
In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei18 – 19Reactive bond for trypsinPROSITE-ProRule annotationBy similarity2
Sitei20 – 21Necessary for sperm bindingBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI01.011

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease inhibitor Kazal-type 1By similarity
Alternative name(s):
Pancreatic secretory trypsin inhibitor1 Publication
Gene namesi
Name:SPINK1
Synonyms:PSTI
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000730271 – 56Serine protease inhibitor Kazal-type 1Add BLAST56

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi9 ↔ 38PROSITE-ProRule annotation1 Publication
Disulfide bondi16 ↔ 35PROSITE-ProRule annotation1 Publication
Disulfide bondi24 ↔ 56PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP00998
PeptideAtlasiP00998
PRIDEiP00998

Expressioni

Gene expression databases

BgeeiENSSSCG00000027244
GenevisibleiP00998 SS

Interactioni

Protein-protein interaction databases

MINTiP00998
STRINGi9823.ENSSSCP00000022179

Structurei

Secondary structure

156
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 17Combined sources6
Beta strandi23 – 25Combined sources3
Beta strandi30 – 33Combined sources4
Helixi34 – 42Combined sources9
Beta strandi50 – 54Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TGSX-ray1.80I1-56[»]
ProteinModelPortaliP00998
SMRiP00998
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00998

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 56Kazal-likePROSITE-ProRule annotationAdd BLAST54

Phylogenomic databases

eggNOGiKOG3649 Eukaryota
ENOG410YC3T LUCA
HOVERGENiHBG006182
InParanoidiP00998
OMAiTKIYNPV
OrthoDBiEOG091G14HG

Family and domain databases

InterProiView protein in InterPro
IPR002350 Kazal_dom
IPR036058 Kazal_dom_sf
IPR001239 Prot_inh_Kazal-m
PfamiView protein in Pfam
PF00050 Kazal_1, 1 hit
PRINTSiPR00290 KAZALINHBTR
SMARTiView protein in SMART
SM00280 KAZAL, 1 hit
SUPFAMiSSF100895 SSF100895, 1 hit
PROSITEiView protein in PROSITE
PS00282 KAZAL_1, 1 hit
PS51465 KAZAL_2, 1 hit

Sequencei

Sequence statusi: Complete.

P00998-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
TSPQREATCT SEVSGCPKIY NPVCGTDGIT YSNECVLCSE NKKRQTPVLI

QKSGPC
Length:56
Mass (Da):6,023
Last modified:July 21, 1986 - v1
Checksum:i39A3649DADF16D25
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti1 – 4Missing in a second inhibitor. 4

Sequence databases

PIRiA91174 TIPG

Similar proteinsi

Entry informationi

Entry nameiISK1_PIG
AccessioniPrimary (citable) accession number: P00998
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 23, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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