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P00998 (ISK1_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pancreatic secretory trypsin inhibitor
Alternative name(s):
Serine protease inhibitor Kazal-type 1
Gene names
Name:SPINK1
Synonyms:PSTI
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length56 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This is a trypsin inhibitor, its physiological function is to prevent the trypsin-catalyzed premature activation of zymogens within the pancreas.

Subcellular location

Secreted.

Sequence similarities

Contains 1 Kazal-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5656Pancreatic secretory trypsin inhibitor
PRO_0000073027

Regions

Domain3 – 5654Kazal-like

Sites

Site18 – 192Reactive bond

Amino acid modifications

Disulfide bond9 ↔ 38 Ref.3
Disulfide bond16 ↔ 35 Ref.3
Disulfide bond24 ↔ 56 Ref.3

Natural variations

Natural variant1 – 44Missing in a second inhibitor.

Secondary structure

.......... 56
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00998 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 39A3649DADF16D25

FASTA566,023
        10         20         30         40         50 
TSPQREATCT SEVSGCPKIY NPVCGTDGIT YSNECVLCSE NKKRQTPVLI QKSGPC 

« Hide

References

[1]"The structure of the porcine pancreatic secretory trypsin inhibitor. I. A sequence determination by Edman degradation and mass spectral identification of the p-bromophenyl-thiohydantoins."
Tschesche H., Wachter E.
Eur. J. Biochem. 16:187-198(1970) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
[2]"The primary structure of the porcine pancreatic secretory trypsin inhibitor. I. Amino acid sequence of the reduced S-aminoethylated protein."
Bartelt D.C., Greene L.J.
J. Biol. Chem. 246:2218-2229(1971) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
[3]"Disulfide bridges of the secretory trypsin inhibitor from procine pancreas and the degradation of covalent structure during the temporary inhibition."
Tschesche H., Schneider M., Reidel G., Klein H.
Hoppe-Seyler's Z. Physiol. Chem. 353:763-764(1972) [PubMed] [Europe PMC] [Abstract]
Cited for: DISULFIDE BONDS.
[4]"Trypsin inhibitors. VII. Primary structure of the specific trypsin inhibitor II (Kazal-type) from porcine pancreas. Sequence analysis with mass spectrometry identification of the p-bromophenylthio-hydantoins from the Edman degradation."
Tschesche H., Wachter E.
Hoppe-Seyler's Z. Physiol. Chem. 351:1449-1459(1970) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF A SECOND INHIBITOR.
[5]"Three-dimensional structure of the complex between pancreatic secretory trypsin inhibitor (Kazal type) and trypsinogen at 1.8-A resolution. Structure solution, crystallographic refinement and preliminary structural interpretation."
Bolognesi M., Gatti G., Menegatti E., Guarneri M., Marquart M., Papamokos E., Huber R.
J. Mol. Biol. 162:839-868(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).

Cross-references

Sequence databases

PIRTIPG. A91174.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1TGSX-ray1.80I1-56[»]
ProteinModelPortalP00998.
SMRP00998. Positions 1-56.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1505130.

Protein family/group databases

MEROPSI01.011.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSSSCT00000026654; ENSSSCP00000022179; ENSSSCG00000027244.

Phylogenomic databases

GeneTreeENSGT00530000064228.
HOVERGENHBG006182.
OMAYANECIL.
OrthoDBEOG7BW0M2.

Family and domain databases

InterProIPR002350. Kazal_dom.
IPR001239. Prot_inh_Kazal-m.
[Graphical view]
PfamPF00050. Kazal_1. 1 hit.
[Graphical view]
PRINTSPR00290. KAZALINHBTR.
SMARTSM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP00998.

Entry information

Entry nameISK1_PIG
AccessionPrimary (citable) accession number: P00998
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 13, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references