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Protein

Kunitz-type serine protease inhibitor homolog alpha-dendrotoxin

Gene
N/A
Organism
Dendroaspis angusticeps (Eastern green mamba) (Naja angusticeps)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease inhibitor homolog that blocks voltage-gated potassium channels (Kv1.1/KCNA1, Kv1.2/KCNA2, and Kv1.6/KCNA6) (IC50=0.4-150 nM) and facilitates neurotransmitter release.2 Publications

Miscellaneous

Does not inhibit serine proteases and voltage-gated potassium channels Kvl.3/KCNA3, Kv1.4/KCNA4, Kv1.5/KCNA5, Kv3.1/KCNC1, Kv3.4/KCNC4, and Kv4.1/KCND1.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei5May be the major determinant of the binding affinity for potassium channels1
Sitei9Important for binding to potassium channels1
Sitei19Not important for inhibition of potassium channels1

GO - Molecular functioni

Keywordsi

Molecular functionIon channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Kunitz-type serine protease inhibitor homolog alpha-dendrotoxin
Short name:
Alpha-DTX
Alternative name(s):
Protease inhibitor 1 homolog
Toxin C13S2C3
Venom basic protease inhibitor 1 homolog
OrganismiDendroaspis angusticeps (Eastern green mamba) (Naja angusticeps)
Taxonomic identifieri8618 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeDendroaspis

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 23 mg/kg by intravenous injection.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001554331 – 59Kunitz-type serine protease inhibitor homolog alpha-dendrotoxinAdd BLAST59

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1Pyrrolidone carboxylic acid1 Publication1
Disulfide bondi7 ↔ 57PROSITE-ProRule annotation1 Publication
Disulfide bondi16 ↔ 40PROSITE-ProRule annotation1 Publication
Disulfide bondi32 ↔ 53PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

159
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 8Combined sources4
Beta strandi15 – 17Combined sources3
Beta strandi20 – 26Combined sources7
Turni27 – 30Combined sources4
Beta strandi31 – 37Combined sources7
Beta strandi47 – 49Combined sources3
Helixi50 – 57Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DTXX-ray2.20A2-59[»]
ProteinModelPortaliP00980.
SMRiP00980.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00980.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 57BPTI/Kunitz inhibitorPROSITE-ProRule annotationAdd BLAST51

Sequence similaritiesi

Belongs to the venom Kunitz-type family.Curated

Phylogenomic databases

HOVERGENiHBG006193.

Family and domain databases

Gene3Di4.10.410.10. 1 hit.
InterProiView protein in InterPro
IPR002223. Kunitz_BPTI.
IPR020901. Prtase_inh_Kunz-CS.
PfamiView protein in Pfam
PF00014. Kunitz_BPTI. 1 hit.
PRINTSiPR00759. BASICPTASE.
SMARTiView protein in SMART
SM00131. KU. 1 hit.
SUPFAMiSSF57362. SSF57362. 1 hit.
PROSITEiView protein in PROSITE
PS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P00980-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
QPRRKLCILH RNPGRCYDKI PAFYYNQKKK QCERFDWSGC GGNSNRFKTI

EECRRTCIG
Length:59
Mass (Da):7,071
Last modified:July 21, 1986 - v1
Checksum:i96B60752E8AD81AE
GO

Sequence databases

PIRiA01212. VIEPIA.

Cross-referencesi

Sequence databases

PIRiA01212. VIEPIA.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DTXX-ray2.20A2-59[»]
ProteinModelPortaliP00980.
SMRiP00980.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG006193.

Miscellaneous databases

EvolutionaryTraceiP00980.

Family and domain databases

Gene3Di4.10.410.10. 1 hit.
InterProiView protein in InterPro
IPR002223. Kunitz_BPTI.
IPR020901. Prtase_inh_Kunz-CS.
PfamiView protein in Pfam
PF00014. Kunitz_BPTI. 1 hit.
PRINTSiPR00759. BASICPTASE.
SMARTiView protein in SMART
SM00131. KU. 1 hit.
SUPFAMiSSF57362. SSF57362. 1 hit.
PROSITEiView protein in PROSITE
PS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVKTHA_DENAN
AccessioniPrimary (citable) accession number: P00980
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: February 15, 2017
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.