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P00980 (VKTHA_DENAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kunitz-type serine protease inhibitor homolog alpha-dendrotoxin

Short name=Alpha-DTX
Alternative name(s):
Protease inhibitor 1 homolog
Toxin C13S2C3
Venom basic protease inhibitor 1 homolog
OrganismDendroaspis angusticeps (Eastern green mamba) (Naja angusticeps)
Taxonomic identifier8618 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeDendroaspis

Protein attributes

Sequence length59 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine protease inhibitor homolog that blocks voltage-gated potassium channels (Kv1.1/KCNA1, Kv1.2/KCNA2, and Kv1.6/KCNA6) (IC50=0.4-150 nM) and facilitates neurotransmitter release. Ref.2 Ref.3

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Toxic dose

LD50 is 23 mg/kg by intravenous injection.

Miscellaneous

Does not inhibit serine proteases and voltage-gated potassium channels Kvl.3/KCNA3, Kv1.4/KCNA4, Kv1.5/KCNA5, Kv3.1/KCNC1, Kv3.4/KCNC4, and Kv4.1/KCND1.

Sequence similarities

Belongs to the venom Kunitz-type family.

Contains 1 BPTI/Kunitz inhibitor domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5959Kunitz-type serine protease inhibitor homolog alpha-dendrotoxin
PRO_0000155433

Regions

Domain7 – 5751BPTI/Kunitz inhibitor

Sites

Site51May be the major determinant of the binding affinity for potassium channels
Site91Important for binding to potassium channels
Site191Not important for inhibition of potassium channels

Amino acid modifications

Modified residue11Pyrrolidone carboxylic acid Ref.1
Disulfide bond7 ↔ 57 Ref.1
Disulfide bond16 ↔ 40 Ref.1
Disulfide bond32 ↔ 53 Ref.1

Secondary structure

............ 59
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00980 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 96B60752E8AD81AE

FASTA597,071
        10         20         30         40         50 
QPRRKLCILH RNPGRCYDKI PAFYYNQKKK QCERFDWSGC GGNSNRFKTI EECRRTCIG 

« Hide

References

[1]"Snake venoms. The amino acid sequences of two proteinase inhibitor homologues from Dendroaspis angusticeps venom."
Joubert F.J., Taljaard N.
Hoppe-Seyler's Z. Physiol. Chem. 361:661-674(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
Tissue: Venom.
[2]"Twenty years of dendrotoxins."
Harvey A.L.
Toxicon 39:15-26(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW, FUNCTION.
[3]"Protease inhibitors from marine venomous animals and their counterparts in terrestrial venomous animals."
Mourao C.B., Schwartz E.F.
Mar. Drugs 11:2069-2112(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW, FUNCTION, SITE LYS-5; LEU-9 AND LYS-19.
[4]"Crystal structure of alpha-dendrotoxin from the green mamba venom and its comparison with the structure of bovine pancreatic trypsin inhibitor."
Skarzynski T.
J. Mol. Biol. 224:671-683(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

Cross-references

Sequence databases

PIRVIEPIA. A01212.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1DTXX-ray2.20A2-59[»]
ProteinModelPortalP00980.
SMRP00980. Positions 1-59.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSI02.056.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG006193.

Family and domain databases

Gene3D4.10.410.10. 1 hit.
InterProIPR002223. Prot_inh_Kunz-m.
IPR020901. Prtase_inh_Kunz-CS.
[Graphical view]
PfamPF00014. Kunitz_BPTI. 1 hit.
[Graphical view]
PRINTSPR00759. BASICPTASE.
SMARTSM00131. KU. 1 hit.
[Graphical view]
SUPFAMSSF57362. SSF57362. 1 hit.
PROSITEPS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP00980.

Entry information

Entry nameVKTHA_DENAN
AccessionPrimary (citable) accession number: P00980
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references