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P00980

- VKTHA_DENAN

UniProt

P00980 - VKTHA_DENAN

Protein

Kunitz-type serine protease inhibitor homolog alpha-dendrotoxin

Gene
N/A
Organism
Dendroaspis angusticeps (Eastern green mamba) (Naja angusticeps)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Serine protease inhibitor homolog that blocks voltage-gated potassium channels (Kv1.1/KCNA1, Kv1.2/KCNA2, and Kv1.6/KCNA6) (IC50=0.4-150 nM) and facilitates neurotransmitter release.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei5 – 51May be the major determinant of the binding affinity for potassium channels
    Sitei9 – 91Important for binding to potassium channels
    Sitei19 – 191Not important for inhibition of potassium channels

    GO - Molecular functioni

    1. serine-type endopeptidase inhibitor activity Source: InterPro

    Keywords - Molecular functioni

    Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

    Protein family/group databases

    MEROPSiI02.056.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kunitz-type serine protease inhibitor homolog alpha-dendrotoxin
    Short name:
    Alpha-DTX
    Alternative name(s):
    Protease inhibitor 1 homolog
    Toxin C13S2C3
    Venom basic protease inhibitor 1 homolog
    OrganismiDendroaspis angusticeps (Eastern green mamba) (Naja angusticeps)
    Taxonomic identifieri8618 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeDendroaspis

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Toxic dosei

    LD50 is 23 mg/kg by intravenous injection.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 5959Kunitz-type serine protease inhibitor homolog alpha-dendrotoxinPRO_0000155433Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11Pyrrolidone carboxylic acid1 Publication
    Disulfide bondi7 ↔ 571 PublicationPROSITE-ProRule annotation
    Disulfide bondi16 ↔ 401 PublicationPROSITE-ProRule annotation
    Disulfide bondi32 ↔ 531 PublicationPROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Pyrrolidone carboxylic acid

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Structurei

    Secondary structure

    1
    59
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 84
    Beta strandi15 – 173
    Beta strandi20 – 267
    Turni27 – 304
    Beta strandi31 – 377
    Beta strandi47 – 493
    Helixi50 – 578

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DTXX-ray2.20A2-59[»]
    ProteinModelPortaliP00980.
    SMRiP00980. Positions 1-59.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00980.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 5751BPTI/Kunitz inhibitorPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the venom Kunitz-type family.Curated
    Contains 1 BPTI/Kunitz inhibitor domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG006193.

    Family and domain databases

    Gene3Di4.10.410.10. 1 hit.
    InterProiIPR002223. Prot_inh_Kunz-m.
    IPR020901. Prtase_inh_Kunz-CS.
    [Graphical view]
    PfamiPF00014. Kunitz_BPTI. 1 hit.
    [Graphical view]
    PRINTSiPR00759. BASICPTASE.
    SMARTiSM00131. KU. 1 hit.
    [Graphical view]
    SUPFAMiSSF57362. SSF57362. 1 hit.
    PROSITEiPS00280. BPTI_KUNITZ_1. 1 hit.
    PS50279. BPTI_KUNITZ_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00980-1 [UniParc]FASTAAdd to Basket

    « Hide

    QPRRKLCILH RNPGRCYDKI PAFYYNQKKK QCERFDWSGC GGNSNRFKTI   50
    EECRRTCIG 59
    Length:59
    Mass (Da):7,071
    Last modified:July 21, 1986 - v1
    Checksum:i96B60752E8AD81AE
    GO

    Sequence databases

    PIRiA01212. VIEPIA.

    Cross-referencesi

    Sequence databases

    PIRi A01212. VIEPIA.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DTX X-ray 2.20 A 2-59 [» ]
    ProteinModelPortali P00980.
    SMRi P00980. Positions 1-59.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi I02.056.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG006193.

    Miscellaneous databases

    EvolutionaryTracei P00980.

    Family and domain databases

    Gene3Di 4.10.410.10. 1 hit.
    InterProi IPR002223. Prot_inh_Kunz-m.
    IPR020901. Prtase_inh_Kunz-CS.
    [Graphical view ]
    Pfami PF00014. Kunitz_BPTI. 1 hit.
    [Graphical view ]
    PRINTSi PR00759. BASICPTASE.
    SMARTi SM00131. KU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57362. SSF57362. 1 hit.
    PROSITEi PS00280. BPTI_KUNITZ_1. 1 hit.
    PS50279. BPTI_KUNITZ_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Snake venoms. The amino acid sequences of two proteinase inhibitor homologues from Dendroaspis angusticeps venom."
      Joubert F.J., Taljaard N.
      Hoppe-Seyler's Z. Physiol. Chem. 361:661-674(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
      Tissue: Venom.
    2. "Twenty years of dendrotoxins."
      Harvey A.L.
      Toxicon 39:15-26(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW, FUNCTION.
    3. "Protease inhibitors from marine venomous animals and their counterparts in terrestrial venomous animals."
      Mourao C.B., Schwartz E.F.
      Mar. Drugs 11:2069-2112(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW, FUNCTION, SITE LYS-5; LEU-9 AND LYS-19.
    4. "Crystal structure of alpha-dendrotoxin from the green mamba venom and its comparison with the structure of bovine pancreatic trypsin inhibitor."
      Skarzynski T.
      J. Mol. Biol. 224:671-683(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

    Entry informationi

    Entry nameiVKTHA_DENAN
    AccessioniPrimary (citable) accession number: P00980
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Does not inhibit serine proteases and voltage-gated potassium channels Kvl.3/KCNA3, Kv1.4/KCNA4, Kv1.5/KCNA5, Kv3.1/KCNC1, Kv3.4/KCNC4, and Kv4.1/KCND1.

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3