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Protein

Pancreatic trypsin inhibitor

Gene
N/A
Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei50 – 512Reactive bond for trypsin

GO - Molecular functioni

GO - Biological processi

  • negative regulation of platelet aggregation Source: CACAO
  • negative regulation of thrombin receptor signaling pathway Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI02.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Pancreatic trypsin inhibitor
Alternative name(s):
Aprotinin
Basic protease inhibitor
Short name:
BPI
Short name:
BPTI
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Pharmaceutical usei

Previously available under the name Trasylol (Mile). Used for inhibiting coagulation so as to reduce blood loss during bypass surgery.

Protein family/group databases

Allergomei3890. Bos d TI.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Propeptidei22 – 3514PRO_0000016852Add
BLAST
Chaini36 – 9358Pancreatic trypsin inhibitorPRO_0000016853Add
BLAST
Propeptidei94 – 1007PRO_0000016854

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi40 ↔ 90
Disulfide bondi49 ↔ 73PROSITE-ProRule annotation1 Publication
Disulfide bondi65 ↔ 86PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP00974.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
P007604EBI-1032263,EBI-986385
Q52V245EBI-1032263,EBI-8755401From a different organism.

Protein-protein interaction databases

DIPiDIP-6113N.
IntActiP00974. 5 interactions.
MINTiMINT-90698.

Structurei

Secondary structure

1
100
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi38 – 414Combined sources
Beta strandi48 – 503Combined sources
Beta strandi53 – 597Combined sources
Turni60 – 634Combined sources
Beta strandi64 – 707Combined sources
Beta strandi72 – 743Combined sources
Beta strandi76 – 783Combined sources
Beta strandi80 – 823Combined sources
Helixi83 – 908Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AALX-ray1.60A/B36-93[»]
1B0CX-ray2.80A/B/C/D/E36-91[»]
1BHCX-ray2.70A/B/C/D/E/F/G/H/I/J36-93[»]
1BPIX-ray1.09A36-93[»]
1BPTX-ray2.00A36-93[»]
1BRBX-ray2.10I36-93[»]
1BTHX-ray2.30P/Q36-93[»]
1BTIX-ray2.20A36-93[»]
1BZ5X-ray2.58A/B/C/D/E36-93[»]
1BZXX-ray2.10I36-93[»]
1CBWX-ray2.60D/I36-93[»]
1CO7X-ray1.90I2-100[»]
1D0DX-ray1.62B36-93[»]
1EAWX-ray2.93B/D36-93[»]
1EJMX-ray1.85B/D/F36-93[»]
1F5RX-ray1.65I36-100[»]
1F7ZX-ray1.55I36-100[»]
1FAKX-ray2.10I37-90[»]
1FANX-ray2.00A36-93[»]
1FY8X-ray1.70I36-93[»]
1G6XX-ray0.86A36-93[»]
1JV8NMR-A36-93[»]
1JV9NMR-A36-93[»]
1K09NMR-A/B50-73[»]
1K6UX-ray1.00A36-93[»]
1LD5NMR-A36-93[»]
1LD6NMR-A36-93[»]
1MTNX-ray2.80D/H36-93[»]
1NAGX-ray1.90A36-93[»]
1OA5NMR-536-93[»]
1OA6NMR-536-93[»]
1P2IX-ray1.65I36-93[»]
1P2JX-ray1.35I36-93[»]
1P2KX-ray1.60I36-93[»]
1P2MX-ray1.75B/D36-93[»]
1P2NX-ray1.80B/D36-93[»]
1P2OX-ray2.00B/D36-93[»]
1P2QX-ray1.80B/D36-93[»]
1PITNMR-A36-93[»]
1QLQX-ray1.42A36-93[»]
1T7CX-ray1.85B/D36-93[»]
1T8LX-ray1.75B/D36-93[»]
1T8MX-ray1.80B/D36-93[»]
1T8NX-ray1.75B/D36-93[»]
1T8OX-ray1.70B/D36-93[»]
1TPAX-ray1.90I36-93[»]
1UUANMR-A38-93[»]
1UUBNMR-A38-93[»]
1YKTX-ray1.70B36-91[»]
1YLCX-ray1.70B36-91[»]
1YLDX-ray1.70B36-91[»]
2FI3X-ray1.58I36-93[»]
2FI4X-ray1.58I36-93[»]
2FI5X-ray1.58I36-93[»]
2FTLX-ray1.62I36-93[»]
2FTMX-ray1.65B36-93[»]
2HEXX-ray2.10A/B/C/D/E36-93[»]
2IJOX-ray2.30I36-93[»]
2KAIX-ray2.50I36-93[»]
2PTCX-ray1.90I36-93[»]
2R9PX-ray1.40E/F/G/I36-93[»]
2RA3X-ray1.46C/I36-93[»]
2TGPX-ray1.90I36-93[»]
2TPIX-ray2.10I36-93[»]
2ZJXX-ray1.09A/B36-93[»]
2ZVXX-ray1.09A/B36-93[»]
3BTDX-ray1.90I36-93[»]
3BTEX-ray1.85I36-93[»]
3BTFX-ray1.80I36-93[»]
3BTGX-ray1.90I36-93[»]
3BTHX-ray1.75I36-93[»]
3BTKX-ray1.85I36-93[»]
3BTMX-ray1.80I36-93[»]
3BTQX-ray1.90I36-93[»]
3BTTX-ray1.90I36-93[»]
3BTWX-ray2.05I36-93[»]
3FP6X-ray1.49I36-93[»]
3FP7X-ray1.46I36-50[»]
J51-93[»]
3FP8X-ray1.46I36-93[»]
3GYMX-ray2.80I/J36-93[»]
3LDIX-ray2.20A/B/C/D/E36-93[»]
3LDJX-ray1.70A/B/C36-93[»]
3LDMX-ray2.60A/B/C/D/E36-93[»]
3OTJOther2.15I36-93[»]
3P92X-ray1.60E36-93[»]
3P95X-ray1.30E36-93[»]
3TGIX-ray1.80I36-100[»]
3TGJX-ray2.20I36-100[»]
3TGKX-ray1.70I36-100[»]
3TPIX-ray1.90I36-93[»]
3U1JX-ray1.80E36-93[»]
3WNYX-ray1.30A/B/C/E/F/G/H/I36-95[»]
4BNRX-ray2.00I/J1-100[»]
4DG4X-ray1.40C/E/F/H36-93[»]
4PTIX-ray1.50A36-93[»]
4TPIX-ray2.20I36-93[»]
4WWYX-ray1.70C/I36-93[»]
4WXVX-ray2.10C/I36-90[»]
4Y0YX-ray1.25I36-93[»]
4Y0ZX-ray1.37I36-93[»]
4Y10X-ray1.37I36-93[»]
4Y11X-ray1.30I36-93[»]
5PTIX-ray1.00A36-93[»]
6PTIX-ray1.70A36-93[»]
7PTIX-ray1.60A36-93[»]
8PTIX-ray1.80A36-93[»]
9PTIX-ray1.22A36-93[»]
DisProtiDP00729.
ProteinModelPortaliP00974.
SMRiP00974. Positions 36-93.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00974.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 9051BPTI/Kunitz inhibitorPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 BPTI/Kunitz inhibitor domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000007327.
HOVERGENiHBG006193.

Family and domain databases

Gene3Di4.10.410.10. 1 hit.
InterProiIPR002223. Kunitz_BPTI.
IPR020901. Prtase_inh_Kunz-CS.
[Graphical view]
PfamiPF00014. Kunitz_BPTI. 1 hit.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00131. KU. 1 hit.
[Graphical view]
SUPFAMiSSF57362. SSF57362. 1 hit.
PROSITEiPS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00974-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMSRLCLSV ALLVLLGTLA ASTPGCDTSN QAKAQRPDFC LEPPYTGPCK
60 70 80 90 100
ARIIRYFYNA KAGLCQTFVY GGCRAKRNNF KSAEDCMRTC GGAIGPWENL
Length:100
Mass (Da):10,903
Last modified:July 1, 1989 - v2
Checksum:i6A778A4AD763FB19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20934, M20930, M20932 Genomic DNA. Translation: AAD13685.1.
X03365 Genomic DNA. Translation: CAA27062.1. Sequence problems.
X03365 Genomic DNA. Translation: CAA27063.1.
X05274 mRNA. Translation: CAA28886.1.
PIRiS00277. TIBO.
UniGeneiBt.102246.
Bt.28518.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20934, M20930, M20932 Genomic DNA. Translation: AAD13685.1.
X03365 Genomic DNA. Translation: CAA27062.1. Sequence problems.
X03365 Genomic DNA. Translation: CAA27063.1.
X05274 mRNA. Translation: CAA28886.1.
PIRiS00277. TIBO.
UniGeneiBt.102246.
Bt.28518.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AALX-ray1.60A/B36-93[»]
1B0CX-ray2.80A/B/C/D/E36-91[»]
1BHCX-ray2.70A/B/C/D/E/F/G/H/I/J36-93[»]
1BPIX-ray1.09A36-93[»]
1BPTX-ray2.00A36-93[»]
1BRBX-ray2.10I36-93[»]
1BTHX-ray2.30P/Q36-93[»]
1BTIX-ray2.20A36-93[»]
1BZ5X-ray2.58A/B/C/D/E36-93[»]
1BZXX-ray2.10I36-93[»]
1CBWX-ray2.60D/I36-93[»]
1CO7X-ray1.90I2-100[»]
1D0DX-ray1.62B36-93[»]
1EAWX-ray2.93B/D36-93[»]
1EJMX-ray1.85B/D/F36-93[»]
1F5RX-ray1.65I36-100[»]
1F7ZX-ray1.55I36-100[»]
1FAKX-ray2.10I37-90[»]
1FANX-ray2.00A36-93[»]
1FY8X-ray1.70I36-93[»]
1G6XX-ray0.86A36-93[»]
1JV8NMR-A36-93[»]
1JV9NMR-A36-93[»]
1K09NMR-A/B50-73[»]
1K6UX-ray1.00A36-93[»]
1LD5NMR-A36-93[»]
1LD6NMR-A36-93[»]
1MTNX-ray2.80D/H36-93[»]
1NAGX-ray1.90A36-93[»]
1OA5NMR-536-93[»]
1OA6NMR-536-93[»]
1P2IX-ray1.65I36-93[»]
1P2JX-ray1.35I36-93[»]
1P2KX-ray1.60I36-93[»]
1P2MX-ray1.75B/D36-93[»]
1P2NX-ray1.80B/D36-93[»]
1P2OX-ray2.00B/D36-93[»]
1P2QX-ray1.80B/D36-93[»]
1PITNMR-A36-93[»]
1QLQX-ray1.42A36-93[»]
1T7CX-ray1.85B/D36-93[»]
1T8LX-ray1.75B/D36-93[»]
1T8MX-ray1.80B/D36-93[»]
1T8NX-ray1.75B/D36-93[»]
1T8OX-ray1.70B/D36-93[»]
1TPAX-ray1.90I36-93[»]
1UUANMR-A38-93[»]
1UUBNMR-A38-93[»]
1YKTX-ray1.70B36-91[»]
1YLCX-ray1.70B36-91[»]
1YLDX-ray1.70B36-91[»]
2FI3X-ray1.58I36-93[»]
2FI4X-ray1.58I36-93[»]
2FI5X-ray1.58I36-93[»]
2FTLX-ray1.62I36-93[»]
2FTMX-ray1.65B36-93[»]
2HEXX-ray2.10A/B/C/D/E36-93[»]
2IJOX-ray2.30I36-93[»]
2KAIX-ray2.50I36-93[»]
2PTCX-ray1.90I36-93[»]
2R9PX-ray1.40E/F/G/I36-93[»]
2RA3X-ray1.46C/I36-93[»]
2TGPX-ray1.90I36-93[»]
2TPIX-ray2.10I36-93[»]
2ZJXX-ray1.09A/B36-93[»]
2ZVXX-ray1.09A/B36-93[»]
3BTDX-ray1.90I36-93[»]
3BTEX-ray1.85I36-93[»]
3BTFX-ray1.80I36-93[»]
3BTGX-ray1.90I36-93[»]
3BTHX-ray1.75I36-93[»]
3BTKX-ray1.85I36-93[»]
3BTMX-ray1.80I36-93[»]
3BTQX-ray1.90I36-93[»]
3BTTX-ray1.90I36-93[»]
3BTWX-ray2.05I36-93[»]
3FP6X-ray1.49I36-93[»]
3FP7X-ray1.46I36-50[»]
J51-93[»]
3FP8X-ray1.46I36-93[»]
3GYMX-ray2.80I/J36-93[»]
3LDIX-ray2.20A/B/C/D/E36-93[»]
3LDJX-ray1.70A/B/C36-93[»]
3LDMX-ray2.60A/B/C/D/E36-93[»]
3OTJOther2.15I36-93[»]
3P92X-ray1.60E36-93[»]
3P95X-ray1.30E36-93[»]
3TGIX-ray1.80I36-100[»]
3TGJX-ray2.20I36-100[»]
3TGKX-ray1.70I36-100[»]
3TPIX-ray1.90I36-93[»]
3U1JX-ray1.80E36-93[»]
3WNYX-ray1.30A/B/C/E/F/G/H/I36-95[»]
4BNRX-ray2.00I/J1-100[»]
4DG4X-ray1.40C/E/F/H36-93[»]
4PTIX-ray1.50A36-93[»]
4TPIX-ray2.20I36-93[»]
4WWYX-ray1.70C/I36-93[»]
4WXVX-ray2.10C/I36-90[»]
4Y0YX-ray1.25I36-93[»]
4Y0ZX-ray1.37I36-93[»]
4Y10X-ray1.37I36-93[»]
4Y11X-ray1.30I36-93[»]
5PTIX-ray1.00A36-93[»]
6PTIX-ray1.70A36-93[»]
7PTIX-ray1.60A36-93[»]
8PTIX-ray1.80A36-93[»]
9PTIX-ray1.22A36-93[»]
DisProtiDP00729.
ProteinModelPortaliP00974.
SMRiP00974. Positions 36-93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6113N.
IntActiP00974. 5 interactions.
MINTiMINT-90698.

Protein family/group databases

Allergomei3890. Bos d TI.
MEROPSiI02.001.

Proteomic databases

PRIDEiP00974.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOGENOMiHOG000007327.
HOVERGENiHBG006193.

Miscellaneous databases

EvolutionaryTraceiP00974.

Family and domain databases

Gene3Di4.10.410.10. 1 hit.
InterProiIPR002223. Kunitz_BPTI.
IPR020901. Prtase_inh_Kunz-CS.
[Graphical view]
PfamiPF00014. Kunitz_BPTI. 1 hit.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00131. KU. 1 hit.
[Graphical view]
SUPFAMiSSF57362. SSF57362. 1 hit.
PROSITEiPS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBPT1_BOVIN
AccessioniPrimary (citable) accession number: P00974
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: September 7, 2016
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Pharmaceutical, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.