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P00967 (PUR2_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trifunctional purine biosynthetic protein adenosine-3

Including the following 3 domains:

  1. Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
    Alternative name(s):
    Glycinamide ribonucleotide synthetase
    Short name=GARS
    Phosphoribosylglycinamide synthetase
  2. Phosphoribosylformylglycinamidine cyclo-ligase
    EC=6.3.3.1
    Alternative name(s):
    AIR synthase
    Short name=AIRS
    Phosphoribosyl-aminoimidazole synthetase
  3. Phosphoribosylglycinamide formyltransferase
    EC=2.1.2.2
    Alternative name(s):
    5'-phosphoribosylglycinamide transformylase
    GAR transformylase
    Short name=GART
Gene names
Name:ade3
Synonyms:Gart
ORF Names:CG31628
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length1353 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00138

10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00138

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.

Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.

Sequence similarities

In the N-terminal section; belongs to the GARS family.

In the central section; belongs to the AIR synthase family.

In the C-terminal section; belongs to the GART family.

Contains 1 ATP-grasp domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P00967-1)

Also known as: A; 4.7 kb;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P00967-2)

Also known as: 1.7 kb;

The sequence of this isoform differs from the canonical sequence as follows:
     434-434: I → M
     435-1353: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13531353Trifunctional purine biosynthetic protein adenosine-3 HAMAP-Rule MF_00138
PRO_0000074934

Regions

Domain114 – 321208ATP-grasp
Nucleotide binding140 – 20263ATP By similarity
Region434 – 1152719AIRS HAMAP-Rule MF_00138
Region1153 – 1353201GART HAMAP-Rule MF_00138
Region1164 – 116635'-phosphoribosylglycinamide binding By similarity
Region1244 – 1247410-formyltetrahydrofolate binding By similarity
Region1295 – 1299510-formyltetrahydrofolate binding By similarity
Region1325 – 132845'-phosphoribosylglycinamide binding By similarity

Sites

Active site12631Proton donor By similarity
Metal binding2911Manganese By similarity
Metal binding2931Manganese By similarity
Binding site1219110-formyltetrahydrofolate By similarity
Binding site1261110-formyltetrahydrofolate By similarity
Site12991Raises pKa of active site His By similarity

Amino acid modifications

Modified residue8141Phosphoserine Ref.6
Modified residue8161Phosphoserine Ref.6

Natural variations

Alternative sequence4341I → M in isoform Short.
VSP_005512
Alternative sequence435 – 1353919Missing in isoform Short.
VSP_005513

Experimental info

Sequence conflict5151F → L in AAF52474. Ref.2
Sequence conflict6021H → Q in AAF52474. Ref.2
Sequence conflict9071G → E in AAF52474. Ref.2
Sequence conflict9601A → D in AAF52474. Ref.2
Sequence conflict11931P → T in AAF52474. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform Long (A) (4.7 kb) [UniParc].

Last modified April 1, 1990. Version 2.
Checksum: A68DAB61A02DFD4F

FASTA1,353144,448
        10         20         30         40         50         60 
MSHRVLVIGS GGREHAICWK LSQSPKVAQI YALPGSHGIQ LVEKCRNLDA KTLDPKDFEA 

        70         80         90        100        110        120 
IAKWSKENQI ALVVVGPEDP LALGLGDVLQ SAGIPCFGPG KQGAQIEADK KWAKDFMLRH 

       130        140        150        160        170        180 
GIPTARYESF TDTEKAKAFI RSAPYPALVV KAAGLAAGKG VVVAANAKEA CQAVDEILGD 

       190        200        210        220        230        240 
LKYGQAGATL VVEELLEGEE VSVLAFTDGK SVRAMLPAQD HKRLGNGDTG PNTGGMGAYC 

       250        260        270        280        290        300 
PCPLISQPAL ELVQKAVLER AVQGLIKERI NYQGVLYAGL MLTRDGPRVL EFNCRFGDPE 

       310        320        330        340        350        360 
TQVILPLLES DLFDVMEACC SGKLDKIPLQ WRNGVSAVGV ILASAGYPET STKGCIISGL 

       370        380        390        400        410        420 
PAANTPTQLV FHSGLAVNAQ KEALTNGGRV LIAIALDGSL KEAAAKATKL AGSISFSGSG 

       430        440        450        460        470        480 
AQYRTDIAQK AFKIASASTP GLSYKDSGVD IDAGDALVQR IKPLSRGTQR PGVIGGLGGF 

       490        500        510        520        530        540 
GGLFRLKELT YKEPVIAEAT QGVGAKIHLA LTHEFYENVG YDLFALAAND VLEVGAEPVA 

       550        560        570        580        590        600 
FLDYIACGKL QVPLAAQLVK GMADGCRDAR CALVGGETAE MPSLYAPGQH DMAGYCVGIV 

       610        620        630        640        650        660 
EHSRILPRFD LYQPGDLLIG LPSSGLHCAG FNEILTQLAA SKVNLRERSP VDGGDDGLTL 

       670        680        690        700        710        720 
AHVLATPTQL YVQQLLPHLQ KGDEIKSVAH VTHGLLNDIL RLLPDGFETT LDFGAVPVPK 

       730        740        750        760        770        780 
IFGWLAGKLK LSAQTILERH NCGIGMVLIL PQSSQLWRTS LPGAKVLGVL QRRSKVSGSP 

       790        800        810        820        830        840 
VQVRNFVEQL EKVASPFGGL GDRELPEELK KLPSNSDLSA PREECFENAA GRRLTRIPTH 

       850        860        870        880        890        900 
YKDPILILGT DGVGTKLKIA QQTNRNTSVG IDLVAMCVND ILCNGAEPIS FSSYYACGHW 

       910        920        930        940        950        960 
QEQLAKGVHS GVQEGARQAN SSFIDSHSAA LPLLYEPQVY DLAGFALGIA EHTGILPLLA 

       970        980        990       1000       1010       1020 
EIQPGDVLIG LPSSGVHSNG FSLVHAVLKR VGLGLHDKAP FSDKTLGEEL LVPTKIYVKA 

      1030       1040       1050       1060       1070       1080 
LSTLLSRGKH GIKALAHITG GGLSENIPRV LRKDLAVRLD ANKFQLPPVF AWLAAAGNIS 

      1090       1100       1110       1120       1130       1140 
STELQRTYNC GLGMVLVVAP TEVEDVLKEL RYPQRAAVVG EVVARKDPKK SQVVVQNFEA 

      1150       1160       1170       1180       1190       1200 
SLARTQKMLS QRRKRVAVLI SGTGSNLQAL IDATRDSAQG IHADVVLVIS NKPGVLGLQR 

      1210       1220       1230       1240       1250       1260 
ATQAGIPSLV ISHKDFASRE VYDAELTRNL KAARVDLICL AGFMRVLSAP FVREWRGRLV 

      1270       1280       1290       1300       1310       1320 
NIHPSLLPKY PGLHVQKQAL EAGEKESGCT VHFVDEGVDT GAIIVQAAVP ILPDDDEDSL 

      1330       1340       1350 
TQRIHKAEHW AFPRALAMLV NGTALISPEV SSQ 

« Hide

Isoform Short (1.7 kb) [UniParc].

Checksum: 0EF3BD499D8B8495
Show »

FASTA43445,864

References

« Hide 'large scale' references
[1]"Conserved arrangement of nested genes at the Drosophila Gart locus."
Henikoff S., Eghtedarzadeh M.K.
Genetics 117:711-725(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS LONG AND SHORT).
Strain: Canton-S.
Tissue: Embryo.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[4]"A Drosophila metabolic gene transcript is alternatively processed."
Henikoff S., Sloan J.S., Kelly J.D.
Cell 34:405-414(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 60-1353, ALTERNATIVE SPLICING.
Strain: Canton-S and Oregon-R.
Tissue: Embryo.
[5]"Sequence of a Drosophila DNA segment that functions in Saccharomyces cerevisiae and its regulation by a yeast promoter."
Henikoff S., Furlong C.E.
Nucleic Acids Res. 11:789-800(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1147-1353 (ISOFORM LONG).
Strain: Oregon-R.
Tissue: Embryo.
[6]"Phosphoproteome analysis of Drosophila melanogaster embryos."
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-814 AND SER-816, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02527 Genomic DNA. Translation: AAA28562.1.
J02527 Genomic DNA. Translation: AAA28563.1.
AE014134 Genomic DNA. Translation: AAF52474.2.
X00041 Genomic DNA. Translation: CAA24923.1.
PIRAJFFPM. S01206.
RefSeqNP_001014477.1. NM_001014477.1. [P00967-2]
NP_523497.2. NM_078773.2.
UniGeneDm.21459.

3D structure databases

ProteinModelPortalP00967.
SMRP00967. Positions 1-431, 442-791, 841-1134, 1155-1343.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid60135. 1 interaction.

Proteomic databases

PaxDbP00967.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID33986.
KEGGdme:Dmel_CG31628.

Organism-specific databases

CTD33986.
FlyBaseFBgn0000053. ade3.

Phylogenomic databases

eggNOGCOG0151.
InParanoidP00967.
KOK11787.
OrthoDBEOG7RBZ7H.
PhylomeDBP00967.

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.
UPA00074; UER00126.
UPA00074; UER00129.

Gene expression databases

BgeeP00967.

Family and domain databases

Gene3D3.30.1330.10. 2 hits.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 2 hits.
HAMAPMF_00138. GARS.
InterProIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsTIGR00877. purD. 1 hit.
TIGR00878. purM. 2 hits.
TIGR00639. PurN. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi33986.
NextBio786281.
PROP00967.

Entry information

Entry namePUR2_DROME
AccessionPrimary (citable) accession number: P00967
Secondary accession number(s): Q9VM53
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1990
Last modified: July 9, 2014
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase