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P00967

- PUR2_DROME

UniProt

P00967 - PUR2_DROME

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Protein

Trifunctional purine biosynthetic protein adenosine-3

Gene
ade3, Gart, CG31628
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi291 – 2911Manganese By similarity
Metal bindingi293 – 2931Manganese By similarity
Binding sitei1219 – 1219110-formyltetrahydrofolate By similarity
Binding sitei1261 – 1261110-formyltetrahydrofolate By similarity
Active sitei1263 – 12631Proton donor By similarity
Sitei1299 – 12991Raises pKa of active site His By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi140 – 20263ATP By similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. methyltransferase activity Source: InterPro
  4. phosphoribosylamine-glycine ligase activity Source: FlyBase
  5. phosphoribosylformylglycinamidine cyclo-ligase activity Source: FlyBase
  6. phosphoribosylglycinamide formyltransferase activity Source: FlyBase

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. purine nucleobase biosynthetic process Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_208157. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00125.
UPA00074; UER00126.
UPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Trifunctional purine biosynthetic protein adenosine-3
Including the following 3 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name:
GART
Gene namesi
Name:ade3
Synonyms:Gart
ORF Names:CG31628
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000053. ade3.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13531353Trifunctional purine biosynthetic protein adenosine-3UniRule annotationPRO_0000074934Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei814 – 8141Phosphoserine1 Publication
Modified residuei816 – 8161Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP00967.

Expressioni

Gene expression databases

BgeeiP00967.

Interactioni

Protein-protein interaction databases

BioGridi60135. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP00967.
SMRiP00967. Positions 1-431, 442-791, 841-1134, 1155-1343.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini114 – 321208ATP-graspAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni434 – 1152719AIRSUniRule annotationAdd
BLAST
Regioni1153 – 1353201GARTUniRule annotationAdd
BLAST
Regioni1164 – 116635'-phosphoribosylglycinamide binding By similarity
Regioni1244 – 1247410-formyltetrahydrofolate binding By similarity
Regioni1295 – 1299510-formyltetrahydrofolate binding By similarity
Regioni1325 – 132845'-phosphoribosylglycinamide binding By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.
In the central section; belongs to the AIR synthase family.
In the C-terminal section; belongs to the GART family.
Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG0151.
InParanoidiP00967.
KOiK11787.
OrthoDBiEOG7RBZ7H.
PhylomeDBiP00967.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 2 hits.
HAMAPiMF_00138. GARS.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 2 hits.
TIGR00639. PurN. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: P00967-1) [UniParc]FASTAAdd to Basket

Also known as: A, 4.7 kb

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSHRVLVIGS GGREHAICWK LSQSPKVAQI YALPGSHGIQ LVEKCRNLDA     50
KTLDPKDFEA IAKWSKENQI ALVVVGPEDP LALGLGDVLQ SAGIPCFGPG 100
KQGAQIEADK KWAKDFMLRH GIPTARYESF TDTEKAKAFI RSAPYPALVV 150
KAAGLAAGKG VVVAANAKEA CQAVDEILGD LKYGQAGATL VVEELLEGEE 200
VSVLAFTDGK SVRAMLPAQD HKRLGNGDTG PNTGGMGAYC PCPLISQPAL 250
ELVQKAVLER AVQGLIKERI NYQGVLYAGL MLTRDGPRVL EFNCRFGDPE 300
TQVILPLLES DLFDVMEACC SGKLDKIPLQ WRNGVSAVGV ILASAGYPET 350
STKGCIISGL PAANTPTQLV FHSGLAVNAQ KEALTNGGRV LIAIALDGSL 400
KEAAAKATKL AGSISFSGSG AQYRTDIAQK AFKIASASTP GLSYKDSGVD 450
IDAGDALVQR IKPLSRGTQR PGVIGGLGGF GGLFRLKELT YKEPVIAEAT 500
QGVGAKIHLA LTHEFYENVG YDLFALAAND VLEVGAEPVA FLDYIACGKL 550
QVPLAAQLVK GMADGCRDAR CALVGGETAE MPSLYAPGQH DMAGYCVGIV 600
EHSRILPRFD LYQPGDLLIG LPSSGLHCAG FNEILTQLAA SKVNLRERSP 650
VDGGDDGLTL AHVLATPTQL YVQQLLPHLQ KGDEIKSVAH VTHGLLNDIL 700
RLLPDGFETT LDFGAVPVPK IFGWLAGKLK LSAQTILERH NCGIGMVLIL 750
PQSSQLWRTS LPGAKVLGVL QRRSKVSGSP VQVRNFVEQL EKVASPFGGL 800
GDRELPEELK KLPSNSDLSA PREECFENAA GRRLTRIPTH YKDPILILGT 850
DGVGTKLKIA QQTNRNTSVG IDLVAMCVND ILCNGAEPIS FSSYYACGHW 900
QEQLAKGVHS GVQEGARQAN SSFIDSHSAA LPLLYEPQVY DLAGFALGIA 950
EHTGILPLLA EIQPGDVLIG LPSSGVHSNG FSLVHAVLKR VGLGLHDKAP 1000
FSDKTLGEEL LVPTKIYVKA LSTLLSRGKH GIKALAHITG GGLSENIPRV 1050
LRKDLAVRLD ANKFQLPPVF AWLAAAGNIS STELQRTYNC GLGMVLVVAP 1100
TEVEDVLKEL RYPQRAAVVG EVVARKDPKK SQVVVQNFEA SLARTQKMLS 1150
QRRKRVAVLI SGTGSNLQAL IDATRDSAQG IHADVVLVIS NKPGVLGLQR 1200
ATQAGIPSLV ISHKDFASRE VYDAELTRNL KAARVDLICL AGFMRVLSAP 1250
FVREWRGRLV NIHPSLLPKY PGLHVQKQAL EAGEKESGCT VHFVDEGVDT 1300
GAIIVQAAVP ILPDDDEDSL TQRIHKAEHW AFPRALAMLV NGTALISPEV 1350
SSQ 1353
Length:1,353
Mass (Da):144,448
Last modified:April 1, 1990 - v2
Checksum:iA68DAB61A02DFD4F
GO
Isoform Short (identifier: P00967-2) [UniParc]FASTAAdd to Basket

Also known as: 1.7 kb

The sequence of this isoform differs from the canonical sequence as follows:
     434-434: I → M
     435-1353: Missing.

Show »
Length:434
Mass (Da):45,864
Checksum:i0EF3BD499D8B8495
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei434 – 4341I → M in isoform Short. VSP_005512
Alternative sequencei435 – 1353919Missing in isoform Short. VSP_005513Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti515 – 5151F → L in AAF52474. 1 Publication
Sequence conflicti602 – 6021H → Q in AAF52474. 1 Publication
Sequence conflicti907 – 9071G → E in AAF52474. 1 Publication
Sequence conflicti960 – 9601A → D in AAF52474. 1 Publication
Sequence conflicti1193 – 11931P → T in AAF52474. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02527 Genomic DNA. Translation: AAA28562.1.
J02527 Genomic DNA. Translation: AAA28563.1.
AE014134 Genomic DNA. Translation: AAF52474.2.
X00041 Genomic DNA. Translation: CAA24923.1.
PIRiS01206. AJFFPM.
RefSeqiNP_001014477.1. NM_001014477.1. [P00967-2]
NP_523497.2. NM_078773.2.
UniGeneiDm.21459.

Genome annotation databases

GeneIDi33986.
KEGGidme:Dmel_CG31628.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02527 Genomic DNA. Translation: AAA28562.1 .
J02527 Genomic DNA. Translation: AAA28563.1 .
AE014134 Genomic DNA. Translation: AAF52474.2 .
X00041 Genomic DNA. Translation: CAA24923.1 .
PIRi S01206. AJFFPM.
RefSeqi NP_001014477.1. NM_001014477.1. [P00967-2 ]
NP_523497.2. NM_078773.2.
UniGenei Dm.21459.

3D structure databases

ProteinModelPortali P00967.
SMRi P00967. Positions 1-431, 442-791, 841-1134, 1155-1343.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 60135. 1 interaction.

Proteomic databases

PaxDbi P00967.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 33986.
KEGGi dme:Dmel_CG31628.

Organism-specific databases

CTDi 33986.
FlyBasei FBgn0000053. ade3.

Phylogenomic databases

eggNOGi COG0151.
InParanoidi P00967.
KOi K11787.
OrthoDBi EOG7RBZ7H.
PhylomeDBi P00967.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00125 .
UPA00074 ; UER00126 .
UPA00074 ; UER00129 .
Reactomei REACT_208157. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

GenomeRNAii 33986.
NextBioi 786281.
PROi P00967.

Gene expression databases

Bgeei P00967.

Family and domain databases

Gene3Di 3.30.1330.10. 2 hits.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 2 hits.
HAMAPi MF_00138. GARS.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsi TIGR00877. purD. 1 hit.
TIGR00878. purM. 2 hits.
TIGR00639. PurN. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Conserved arrangement of nested genes at the Drosophila Gart locus."
    Henikoff S., Eghtedarzadeh M.K.
    Genetics 117:711-725(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS LONG AND SHORT).
    Strain: Canton-S.
    Tissue: Embryo.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. "A Drosophila metabolic gene transcript is alternatively processed."
    Henikoff S., Sloan J.S., Kelly J.D.
    Cell 34:405-414(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 60-1353, ALTERNATIVE SPLICING.
    Strain: Canton-S and Oregon-R.
    Tissue: Embryo.
  5. "Sequence of a Drosophila DNA segment that functions in Saccharomyces cerevisiae and its regulation by a yeast promoter."
    Henikoff S., Furlong C.E.
    Nucleic Acids Res. 11:789-800(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1147-1353 (ISOFORM LONG).
    Strain: Oregon-R.
    Tissue: Embryo.
  6. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-814 AND SER-816, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiPUR2_DROME
AccessioniPrimary (citable) accession number: P00967
Secondary accession number(s): Q9VM53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1990
Last modified: September 3, 2014
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi