P00967 (PUR2_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trifunctional purine biosynthetic protein adenosine-3

Including the following 3 domains:

1. Phosphoribosylamine--glycine ligase
   EC=6.3.4.13
   Alternative name(s):
   Glycinamide ribonucleotide synthetase
   Short name=GARS
   Phosphoribosylglycinamide synthetase
2. Phosphoribosylformylglycinamidine cyclo-ligase
   EC=6.3.3.1
   Alternative name(s):
   AIR synthase
   Short name=AIRS
   Phosphoribosyl-aminoimidazole synthetase
3. Phosphoribosylglycinamide formyltransferase
   EC=2.1.2.2
   Alternative name(s):
   5'-phosphoribosylglycinamide transformylase
   GAR transformylase
   Short name=GART

Gene names

Name:	ade3
Synonyms:	Gart
ORF Names:	CG31628

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1353 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. 10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Sequence similarities	In the N-terminal section; belongs to the GARS family. In the central section; belongs to the AIR synthase family. In the C-terminal section; belongs to the GART family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro purine base biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methyltransferase activity Inferred from electronic annotation. Source: InterPro phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: EC phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC phosphoribosylglycinamide formyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P00967-1)

Also known as: A; 4.7 kb;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P00967-2)

Also known as: 1.7 kb;

The sequence of this isoform differs from the canonical sequence as follows:
     434-434: I → M
     435-1353: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1353	1353	Trifunctional purine biosynthetic protein adenosine-3		PRO_0000074934
Regions
	Domain	114 – 321	208	ATP-grasp
	Nucleotide binding	140 – 202	63	ATP By similarity
	Region	434 – 1152	719	AIRS
	Region	1153 – 1353	201	GART
	Region	1164 – 1166	3	5'-phosphoribosylglycinamide binding By similarity
	Region	1244 – 1247	4	10-formyltetrahydrofolate binding By similarity
	Region	1295 – 1299	5	10-formyltetrahydrofolate binding By similarity
	Region	1325 – 1328	4	5'-phosphoribosylglycinamide binding By similarity
Sites
	Active site	1263	1	Proton donor By similarity
	Metal binding	291	1	Manganese By similarity
	Metal binding	293	1	Manganese By similarity
	Binding site	1219	1	10-formyltetrahydrofolate By similarity
	Binding site	1261	1	10-formyltetrahydrofolate By similarity
	Site	1299	1	Raises pKa of active site His By similarity
Amino acid modifications
	Modified residue	814	1	Phosphoserine Ref.6
	Modified residue	816	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	434	1	I → M in isoform Short.		VSP_005512
	Alternative sequence	435 – 1353	919	Missing in isoform Short.		VSP_005513
Experimental info
	Sequence conflict	515	1	F → L in AAF52474. Ref.2
	Sequence conflict	602	1	H → Q in AAF52474. Ref.2
	Sequence conflict	907	1	G → E in AAF52474. Ref.2
	Sequence conflict	960	1	A → D in AAF52474. Ref.2
	Sequence conflict	1193	1	P → T in AAF52474. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long (A) (4.7 kb) [UniParc]. Last modified April 1, 1990. Version 2. Checksum: A68DAB61A02DFD4F	FASTA	1,353	144,448
	10 20 30 40 50 60 MSHRVLVIGS GGREHAICWK LSQSPKVAQI YALPGSHGIQ LVEKCRNLDA KTLDPKDFEA 70 80 90 100 110 120 IAKWSKENQI ALVVVGPEDP LALGLGDVLQ SAGIPCFGPG KQGAQIEADK KWAKDFMLRH 130 140 150 160 170 180 GIPTARYESF TDTEKAKAFI RSAPYPALVV KAAGLAAGKG VVVAANAKEA CQAVDEILGD 190 200 210 220 230 240 LKYGQAGATL VVEELLEGEE VSVLAFTDGK SVRAMLPAQD HKRLGNGDTG PNTGGMGAYC 250 260 270 280 290 300 PCPLISQPAL ELVQKAVLER AVQGLIKERI NYQGVLYAGL MLTRDGPRVL EFNCRFGDPE 310 320 330 340 350 360 TQVILPLLES DLFDVMEACC SGKLDKIPLQ WRNGVSAVGV ILASAGYPET STKGCIISGL 370 380 390 400 410 420 PAANTPTQLV FHSGLAVNAQ KEALTNGGRV LIAIALDGSL KEAAAKATKL AGSISFSGSG 430 440 450 460 470 480 AQYRTDIAQK AFKIASASTP GLSYKDSGVD IDAGDALVQR IKPLSRGTQR PGVIGGLGGF 490 500 510 520 530 540 GGLFRLKELT YKEPVIAEAT QGVGAKIHLA LTHEFYENVG YDLFALAAND VLEVGAEPVA 550 560 570 580 590 600 FLDYIACGKL QVPLAAQLVK GMADGCRDAR CALVGGETAE MPSLYAPGQH DMAGYCVGIV 610 620 630 640 650 660 EHSRILPRFD LYQPGDLLIG LPSSGLHCAG FNEILTQLAA SKVNLRERSP VDGGDDGLTL 670 680 690 700 710 720 AHVLATPTQL YVQQLLPHLQ KGDEIKSVAH VTHGLLNDIL RLLPDGFETT LDFGAVPVPK 730 740 750 760 770 780 IFGWLAGKLK LSAQTILERH NCGIGMVLIL PQSSQLWRTS LPGAKVLGVL QRRSKVSGSP 790 800 810 820 830 840 VQVRNFVEQL EKVASPFGGL GDRELPEELK KLPSNSDLSA PREECFENAA GRRLTRIPTH 850 860 870 880 890 900 YKDPILILGT DGVGTKLKIA QQTNRNTSVG IDLVAMCVND ILCNGAEPIS FSSYYACGHW 910 920 930 940 950 960 QEQLAKGVHS GVQEGARQAN SSFIDSHSAA LPLLYEPQVY DLAGFALGIA EHTGILPLLA 970 980 990 1000 1010 1020 EIQPGDVLIG LPSSGVHSNG FSLVHAVLKR VGLGLHDKAP FSDKTLGEEL LVPTKIYVKA 1030 1040 1050 1060 1070 1080 LSTLLSRGKH GIKALAHITG GGLSENIPRV LRKDLAVRLD ANKFQLPPVF AWLAAAGNIS 1090 1100 1110 1120 1130 1140 STELQRTYNC GLGMVLVVAP TEVEDVLKEL RYPQRAAVVG EVVARKDPKK SQVVVQNFEA 1150 1160 1170 1180 1190 1200 SLARTQKMLS QRRKRVAVLI SGTGSNLQAL IDATRDSAQG IHADVVLVIS NKPGVLGLQR 1210 1220 1230 1240 1250 1260 ATQAGIPSLV ISHKDFASRE VYDAELTRNL KAARVDLICL AGFMRVLSAP FVREWRGRLV 1270 1280 1290 1300 1310 1320 NIHPSLLPKY PGLHVQKQAL EAGEKESGCT VHFVDEGVDT GAIIVQAAVP ILPDDDEDSL 1330 1340 1350 TQRIHKAEHW AFPRALAMLV NGTALISPEV SSQ
	Isoform Short (1.7 kb) [UniParc]. Checksum: 0EF3BD499D8B8495	FASTA	434	45,864
	10 20 30 40 50 60 MSHRVLVIGS GGREHAICWK LSQSPKVAQI YALPGSHGIQ LVEKCRNLDA KTLDPKDFEA 70 80 90 100 110 120 IAKWSKENQI ALVVVGPEDP LALGLGDVLQ SAGIPCFGPG KQGAQIEADK KWAKDFMLRH 130 140 150 160 170 180 GIPTARYESF TDTEKAKAFI RSAPYPALVV KAAGLAAGKG VVVAANAKEA CQAVDEILGD 190 200 210 220 230 240 LKYGQAGATL VVEELLEGEE VSVLAFTDGK SVRAMLPAQD HKRLGNGDTG PNTGGMGAYC 250 260 270 280 290 300 PCPLISQPAL ELVQKAVLER AVQGLIKERI NYQGVLYAGL MLTRDGPRVL EFNCRFGDPE 310 320 330 340 350 360 TQVILPLLES DLFDVMEACC SGKLDKIPLQ WRNGVSAVGV ILASAGYPET STKGCIISGL 370 380 390 400 410 420 PAANTPTQLV FHSGLAVNAQ KEALTNGGRV LIAIALDGSL KEAAAKATKL AGSISFSGSG 430 AQYRTDIAQK AFKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Conserved arrangement of nested genes at the Drosophila Gart locus." Henikoff S., Eghtedarzadeh M.K. Genetics 117:711-725(1987) [PubMed: 3123310] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS LONG AND SHORT). Strain: Canton-S. Tissue: Embryo.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila metabolic gene transcript is alternatively processed." Henikoff S., Sloan J.S., Kelly J.D. Cell 34:405-414(1983) [PubMed: 6413075] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 60-1353, ALTERNATIVE SPLICING. Strain: Canton-S and Oregon-R. Tissue: Embryo.
[5]	"Sequence of a Drosophila DNA segment that functions in Saccharomyces cerevisiae and its regulation by a yeast promoter." Henikoff S., Furlong C.E. Nucleic Acids Res. 11:789-800(1983) [PubMed: 6300768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1147-1353 (ISOFORM LONG). Strain: Oregon-R. Tissue: Embryo.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-814 AND SER-816, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02527 Genomic DNA. Translation: AAA28562.1. J02527 Genomic DNA. Translation: AAA28563.1. AE014134 Genomic DNA. Translation: AAF52474.2. X00041 Genomic DNA. Translation: CAA24923.1.
PIR	AJFFPM. S01206.
RefSeq	NP_001014477.1. NM_001014477.1. NP_523497.2. NM_078773.2.
UniGene	Dm.21459.
3D structure databases
ProteinModelPortal	P00967.
SMR	P00967. Positions 1-435, 440-784, 830-1144, 1154-1352.
ModBase	Search...
Protein-protein interaction databases
STRING	P00967.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	33986.
KEGG	dme:Dmel_CG31628.
Organism-specific databases
CTD	33986.
FlyBase	FBgn0000053. ade3.
Phylogenomic databases
eggNOG	inNOG08032.
InParanoid	P00967.
OrthoDB	EOG4THT80.
PhylomeDB	P00967.
Gene expression databases
Bgee	P00967.
GermOnline	CG31628. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR002376. Formyl_transf_N. IPR001555. GART_AS. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR004733. PurM_cligase. IPR016188. PurM_N-like. IPR004607. PurN_trans. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.170. Formyl_transf_N. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K11787.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. PF00551. Formyl_trans_N. 1 hit. PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF53328. formyl_transf. 1 hit. SSF52440. PreATP-grasp-like. 1 hit. SSF55326. PurM_N-like. 2 hits. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. PurD. 1 hit. TIGR00878. PurM. 2 hits. TIGR00639. PurN. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	786281.

Entry information

Entry name

PUR2_DROME

Accession

Primary (citable) accession number: P00967
Secondary accession number(s): Q9VM53

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	April 1, 1990
Last modified:	January 25, 2012
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project