Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P00965

- GLNA3_PHAVU

UniProt

P00965 - GLNA3_PHAVU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glutamine synthetase N-1

Gene

Gln-gamma

Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase N-1 (EC:6.3.1.2)
Alternative name(s):
Gln isozyme gamma
Glutamate--ammonia ligase
Gene namesi
Name:Gln-gamma
OrganismiPhaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifieri3885 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Glutamine synthetase N-1PRO_0000153193Add
BLAST

Proteomic databases

ProMEXiP00965.

Expressioni

Tissue specificityi

This is a nodule isozyme.

Interactioni

Subunit structurei

Homooctamer.

Structurei

3D structure databases

ProteinModelPortaliP00965.
SMRiP00965. Positions 4-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00965-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSISDLVNL NLSDSTERVI AEYIWVGGSG MDMRSKARTL SGPVKDPSKL
60 70 80 90 100
PKWNYDGSST GQAPGQDSEV ILYPQTIFRD PFRRGNNILV MCDAYTPAGE
110 120 130 140 150
PIPTNKRHNA AKIFSNPEVV AEEPWYGIEQ EYTLLQKDVQ WPVGWPLGGF
160 170 180 190 200
PGPQGPYYCG IGANKAFGRD IVDSHYKACL YAGINISGIN GEVMPGQWEF
210 220 230 240 250
QVGPSVGISA ADELWVARYI LERITEIAGV VLSFDPKPIQ GDWNGAGAHT
260 270 280 290 300
NYSTKSMRND GGYEVIKKAI TKLEKRHKEH IAAYGEGNER RLTGKHETAD
310 320 330 340 350
MNTFIWGVAN RGASIRVGRD TEKAGKGYFE DRRPASNMDP YVVTSMIAET

TLLWKP
Length:356
Mass (Da):39,341
Last modified:October 1, 1989 - v2
Checksum:i3C88504756A452DC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14605 mRNA. Translation: CAA32759.1.
M10159 mRNA. Translation: AAA33762.1.
M92095 Genomic DNA. Translation: AAA33761.1.
PIRiS04727. AJFBQ.
RefSeqiXP_007141923.1. XM_007141861.1.

Genome annotation databases

GeneIDi18623838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14605 mRNA. Translation: CAA32759.1 .
M10159 mRNA. Translation: AAA33762.1 .
M92095 Genomic DNA. Translation: AAA33761.1 .
PIRi S04727. AJFBQ.
RefSeqi XP_007141923.1. XM_007141861.1.

3D structure databases

ProteinModelPortali P00965.
SMRi P00965. Positions 4-355.
ModBasei Search...
MobiDBi Search...

Proteomic databases

ProMEXi P00965.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 18623838.

Family and domain databases

Gene3Di 3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA sequence and differential expression of the gene encoding the glutamine synthetase and polypeptide of Phaseolus vulgaris L."
    Bennett M.J., Lightfoot D.A., Cullimore J.V.
    Plant Mol. Biol. 12:553-565(1989)
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Root nodule.
  2. "Glutamine synthetase of Phaseolus vulgaris L.: organ-specific expression of a multigene family."
    Cullimore J.V., Gebhardt C., Saarelainen R., Miflin B.J., Idler K.B., Barker R.F.
    J. Mol. Appl. Genet. 2:589-599(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 216-356.
  3. "Nuclear factors interact with conserved A/T-rich elements upstream of a nodule-enhanced glutamine synthetase gene from French bean."
    Forde B.G., Freeman J., Oliver J.E., Pineda M.
    Plant Cell 2:925-939(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1-38.

Entry informationi

Entry nameiGLNA3_PHAVU
AccessioniPrimary (citable) accession number: P00965
Secondary accession number(s): Q9SAT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 1, 1989
Last modified: October 29, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are at least four isozymes of this enzyme in P.vulgaris.
Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3