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P00965 (GLNA3_PHAVU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase N-1

EC=6.3.1.2
Alternative name(s):
Gln isozyme gamma
Glutamate--ammonia ligase
Gene names
Name:Gln-gamma
OrganismPhaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifier3885 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer.

Subcellular location

Cytoplasm.

Tissue specificity

This is a nodule isozyme.

Miscellaneous

There are at least four isozymes of this enzyme in P.vulgaris.

Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Biological processNitrogen fixation
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 356356Glutamine synthetase N-1
PRO_0000153193

Sequences

Sequence LengthMass (Da)Tools
P00965 [UniParc].

Last modified October 1, 1989. Version 2.
Checksum: 3C88504756A452DC

FASTA35639,341
        10         20         30         40         50         60 
MSSISDLVNL NLSDSTERVI AEYIWVGGSG MDMRSKARTL SGPVKDPSKL PKWNYDGSST 

        70         80         90        100        110        120 
GQAPGQDSEV ILYPQTIFRD PFRRGNNILV MCDAYTPAGE PIPTNKRHNA AKIFSNPEVV 

       130        140        150        160        170        180 
AEEPWYGIEQ EYTLLQKDVQ WPVGWPLGGF PGPQGPYYCG IGANKAFGRD IVDSHYKACL 

       190        200        210        220        230        240 
YAGINISGIN GEVMPGQWEF QVGPSVGISA ADELWVARYI LERITEIAGV VLSFDPKPIQ 

       250        260        270        280        290        300 
GDWNGAGAHT NYSTKSMRND GGYEVIKKAI TKLEKRHKEH IAAYGEGNER RLTGKHETAD 

       310        320        330        340        350 
MNTFIWGVAN RGASIRVGRD TEKAGKGYFE DRRPASNMDP YVVTSMIAET TLLWKP 

« Hide

References

[1]"cDNA sequence and differential expression of the gene encoding the glutamine synthetase and polypeptide of Phaseolus vulgaris L."
Bennett M.J., Lightfoot D.A., Cullimore J.V.
Plant Mol. Biol. 12:553-565(1989)
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Root nodule.
[2]"Glutamine synthetase of Phaseolus vulgaris L.: organ-specific expression of a multigene family."
Cullimore J.V., Gebhardt C., Saarelainen R., Miflin B.J., Idler K.B., Barker R.F.
J. Mol. Appl. Genet. 2:589-599(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 216-356.
[3]"Nuclear factors interact with conserved A/T-rich elements upstream of a nodule-enhanced glutamine synthetase gene from French bean."
Forde B.G., Freeman J., Oliver J.E., Pineda M.
Plant Cell 2:925-939(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 1-38.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14605 mRNA. Translation: CAA32759.1.
M10159 mRNA. Translation: AAA33762.1.
M92095 Genomic DNA. Translation: AAA33761.1.
PIRAJFBQ. S04727.

3D structure databases

ProteinModelPortalP00965.
SMRP00965. Positions 4-355.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

ProMEXP00965.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA3_PHAVU
AccessionPrimary (citable) accession number: P00965
Secondary accession number(s): Q9SAT5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 1, 1989
Last modified: February 19, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families