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P00965

- GLNA3_PHAVU

UniProt

P00965 - GLNA3_PHAVU

Protein

Glutamine synthetase N-1

Gene

Gln-gamma

Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 2 (01 Oct 1989)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. nitrogen fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Nitrogen fixation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase N-1 (EC:6.3.1.2)
    Alternative name(s):
    Gln isozyme gamma
    Glutamate--ammonia ligase
    Gene namesi
    Name:Gln-gamma
    OrganismiPhaseolus vulgaris (Kidney bean) (French bean)
    Taxonomic identifieri3885 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 356356Glutamine synthetase N-1PRO_0000153193Add
    BLAST

    Proteomic databases

    ProMEXiP00965.

    Expressioni

    Tissue specificityi

    This is a nodule isozyme.

    Interactioni

    Subunit structurei

    Homooctamer.

    Structurei

    3D structure databases

    ProteinModelPortaliP00965.
    SMRiP00965. Positions 4-355.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00965-1 [UniParc]FASTAAdd to Basket

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    MSSISDLVNL NLSDSTERVI AEYIWVGGSG MDMRSKARTL SGPVKDPSKL    50
    PKWNYDGSST GQAPGQDSEV ILYPQTIFRD PFRRGNNILV MCDAYTPAGE 100
    PIPTNKRHNA AKIFSNPEVV AEEPWYGIEQ EYTLLQKDVQ WPVGWPLGGF 150
    PGPQGPYYCG IGANKAFGRD IVDSHYKACL YAGINISGIN GEVMPGQWEF 200
    QVGPSVGISA ADELWVARYI LERITEIAGV VLSFDPKPIQ GDWNGAGAHT 250
    NYSTKSMRND GGYEVIKKAI TKLEKRHKEH IAAYGEGNER RLTGKHETAD 300
    MNTFIWGVAN RGASIRVGRD TEKAGKGYFE DRRPASNMDP YVVTSMIAET 350
    TLLWKP 356
    Length:356
    Mass (Da):39,341
    Last modified:October 1, 1989 - v2
    Checksum:i3C88504756A452DC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14605 mRNA. Translation: CAA32759.1.
    M10159 mRNA. Translation: AAA33762.1.
    M92095 Genomic DNA. Translation: AAA33761.1.
    PIRiS04727. AJFBQ.
    RefSeqiXP_007141923.1. XM_007141861.1.

    Genome annotation databases

    GeneIDi18623838.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14605 mRNA. Translation: CAA32759.1 .
    M10159 mRNA. Translation: AAA33762.1 .
    M92095 Genomic DNA. Translation: AAA33761.1 .
    PIRi S04727. AJFBQ.
    RefSeqi XP_007141923.1. XM_007141861.1.

    3D structure databases

    ProteinModelPortali P00965.
    SMRi P00965. Positions 4-355.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    ProMEXi P00965.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 18623838.

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA sequence and differential expression of the gene encoding the glutamine synthetase and polypeptide of Phaseolus vulgaris L."
      Bennett M.J., Lightfoot D.A., Cullimore J.V.
      Plant Mol. Biol. 12:553-565(1989)
      Cited for: NUCLEOTIDE SEQUENCE.
      Tissue: Root nodule.
    2. "Glutamine synthetase of Phaseolus vulgaris L.: organ-specific expression of a multigene family."
      Cullimore J.V., Gebhardt C., Saarelainen R., Miflin B.J., Idler K.B., Barker R.F.
      J. Mol. Appl. Genet. 2:589-599(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 216-356.
    3. "Nuclear factors interact with conserved A/T-rich elements upstream of a nodule-enhanced glutamine synthetase gene from French bean."
      Forde B.G., Freeman J., Oliver J.E., Pineda M.
      Plant Cell 2:925-939(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 1-38.

    Entry informationi

    Entry nameiGLNA3_PHAVU
    AccessioniPrimary (citable) accession number: P00965
    Secondary accession number(s): Q9SAT5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 77 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are at least four isozymes of this enzyme in P.vulgaris.
    Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3