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P00964

- GLNA_NOSS1

UniProt

P00964 - GLNA_NOSS1

Protein

Glutamine synthetase

Gene

glnA

Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    Enzyme regulationi

    The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive By similarity.By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. heterocyst differentiation Source: UniProtKB-KW
    3. nitrogen fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Nitrogen fixation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase
    Gene namesi
    Name:glnA
    Ordered Locus Names:alr2328
    OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
    Taxonomic identifieri103690 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
    ProteomesiUP000002483: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 474473Glutamine synthetasePRO_0000153215Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei402 – 4021O-AMP-tyrosineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Keywords - Developmental stagei

    Heterocyst

    Interactioni

    Subunit structurei

    Oligomer of 12 subunits arranged in the form of two hexagons.

    Protein-protein interaction databases

    STRINGi103690.alr2328.

    Structurei

    3D structure databases

    ProteinModelPortaliP00964.
    SMRiP00964. Positions 2-474.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0174.
    HOGENOMiHOG000005157.
    KOiK01915.
    OMAiYESGMNS.
    OrthoDBiEOG6B360N.

    Family and domain databases

    Gene3Di3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR004809. Gln_synth_I.
    IPR001637. Gln_synth_I_adenylation_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    TIGRFAMsiTIGR00653. GlnA. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00182. GLNA_ADENYLATION. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00964-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTPQEVLKR IQDEKIELID LKFIDTVGTW QHLTLYQNQI DESSFSDGVP    50
    FDGSSIRGWK AINESDMTMV LDPNTAWIDP FMEVPTLSIV CSIKEPRTGE 100
    WYNRCPRVIA QKAIDYLVST GIGDTAFFGP EAEFFIFDSA RFAQNANEGY 150
    YFLDSVEGAW NSGKEGTADK PNLAYKPRFK EGYFPVSPTD SFQDIRTEML 200
    LTMAKLGVPI EKHHHEVATG GQCELGFRFG KLIEAADWLM IYKYVIKNVA 250
    KKYGKTVTFM PKPIFGDNGS GMHCHQSIWK DGKPLFAGDQ YAGLSEMGLY 300
    YIGGLLKHAP ALLAITNPST NSYKRLVPGY EAPVNLAYSQ GNRSASIRIP 350
    LSGTNPKAKR LEFRCPDATS NPYLAFAAML CAGIDGIKNK IHPGEPLDKN 400
    IYELSPEELA KVPSTPGSLE LALEALENDH AFLTDTGVFT EDFIQNWIDY 450
    KLANEVKQMQ LRPHPYEFSI YYDV 474
    Length:474
    Mass (Da):53,136
    Last modified:January 23, 2007 - v3
    Checksum:i6A801A1B55262B28
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti107 – 1071R → P in CAA24982. 1 PublicationCurated
    Sequence conflicti177 – 1771Missing AA sequence 1 PublicationCurated

    Mass spectrometryi

    Molecular mass is 53314 Da from positions 2 - 474. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00147 Genomic DNA. Translation: CAA24982.1.
    BA000019 Genomic DNA. Translation: BAB74027.1.
    PIRiA01192. AJAIQ.
    AI2096.
    RefSeqiNP_486368.1. NC_003272.1.
    WP_010996484.1. NC_003272.1.

    Genome annotation databases

    EnsemblBacteriaiBAB74027; BAB74027; BAB74027.
    GeneIDi1105925.
    KEGGiana:alr2328.
    PATRICi22774830. VBINosSp37423_2882.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00147 Genomic DNA. Translation: CAA24982.1 .
    BA000019 Genomic DNA. Translation: BAB74027.1 .
    PIRi A01192. AJAIQ.
    AI2096.
    RefSeqi NP_486368.1. NC_003272.1.
    WP_010996484.1. NC_003272.1.

    3D structure databases

    ProteinModelPortali P00964.
    SMRi P00964. Positions 2-474.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 103690.alr2328.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB74027 ; BAB74027 ; BAB74027 .
    GeneIDi 1105925.
    KEGGi ana:alr2328.
    PATRICi 22774830. VBINosSp37423_2882.

    Phylogenomic databases

    eggNOGi COG0174.
    HOGENOMi HOG000005157.
    KOi K01915.
    OMAi YESGMNS.
    OrthoDBi EOG6B360N.

    Family and domain databases

    Gene3Di 3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR004809. Gln_synth_I.
    IPR001637. Gln_synth_I_adenylation_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    TIGRFAMsi TIGR00653. GlnA. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00182. GLNA_ADENYLATION. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Different promoters for the Anabaena glutamine synthetase gene during growth usine molecular or fixed nitrogen."
      Tumer N.E., Robinson S.J., Haselkorn R.
      Nature 306:337-342(1983)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 7120 / UTEX 2576.
    3. Singh H., Rajaram H., Apte S.K.
      Submitted (DEC-2008) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 23-57; 98-104; 142-196; 213-243; 326-343 AND 391-411, MASS SPECTROMETRY.

    Entry informationi

    Entry nameiGLNA_NOSS1
    AccessioniPrimary (citable) accession number: P00964
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 100 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In Anabaena, it is present in ammonia-grown vegetative cells as well as in heterocysts (for nitrogen fixation).

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3