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P00964 (GLNA_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase

EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase
Gene names
Name:glnA
Ordered Locus Names:alr2328
OrganismNostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]
Taxonomic identifier103690 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Enzyme regulation

The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive By similarity.

Subunit structure

Oligomer of 12 subunits arranged in the form of two hexagons.

Subcellular location

Cytoplasm.

Miscellaneous

In Anabaena, it is present in ammonia-grown vegetative cells as well as in heterocysts (for nitrogen fixation).

Sequence similarities

Belongs to the glutamine synthetase family.

Mass spectrometry

Molecular mass is 53314 Da from positions 2 - 474. Determined by MALDI. Ref.3

Ontologies

Keywords
   Biological processNitrogen fixation
   Cellular componentCytoplasm
   Developmental stageHeterocyst
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

heterocyst differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

nitrogen fixation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 474473Glutamine synthetase
PRO_0000153215

Amino acid modifications

Modified residue4021O-AMP-tyrosine By similarity

Experimental info

Sequence conflict1071R → P in CAA24982. Ref.1
Sequence conflict1771Missing AA sequence Ref.3

Sequences

Sequence LengthMass (Da)Tools
P00964 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 6A801A1B55262B28

FASTA47453,136
        10         20         30         40         50         60 
MTTPQEVLKR IQDEKIELID LKFIDTVGTW QHLTLYQNQI DESSFSDGVP FDGSSIRGWK 

        70         80         90        100        110        120 
AINESDMTMV LDPNTAWIDP FMEVPTLSIV CSIKEPRTGE WYNRCPRVIA QKAIDYLVST 

       130        140        150        160        170        180 
GIGDTAFFGP EAEFFIFDSA RFAQNANEGY YFLDSVEGAW NSGKEGTADK PNLAYKPRFK 

       190        200        210        220        230        240 
EGYFPVSPTD SFQDIRTEML LTMAKLGVPI EKHHHEVATG GQCELGFRFG KLIEAADWLM 

       250        260        270        280        290        300 
IYKYVIKNVA KKYGKTVTFM PKPIFGDNGS GMHCHQSIWK DGKPLFAGDQ YAGLSEMGLY 

       310        320        330        340        350        360 
YIGGLLKHAP ALLAITNPST NSYKRLVPGY EAPVNLAYSQ GNRSASIRIP LSGTNPKAKR 

       370        380        390        400        410        420 
LEFRCPDATS NPYLAFAAML CAGIDGIKNK IHPGEPLDKN IYELSPEELA KVPSTPGSLE 

       430        440        450        460        470 
LALEALENDH AFLTDTGVFT EDFIQNWIDY KLANEVKQMQ LRPHPYEFSI YYDV 

« Hide

References

« Hide 'large scale' references
[1]"Different promoters for the Anabaena glutamine synthetase gene during growth usine molecular or fixed nitrogen."
Tumer N.E., Robinson S.J., Haselkorn R.
Nature 306:337-342(1983)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120."
Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. expand/collapse author list , Takazawa M., Yamada M., Yasuda M., Tabata S.
DNA Res. 8:205-213(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7120 / UTEX 2576.
[3]Singh H., Rajaram H., Apte S.K.
Submitted (DEC-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 23-57; 98-104; 142-196; 213-243; 326-343 AND 391-411, MASS SPECTROMETRY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X00147 Genomic DNA. Translation: CAA24982.1.
BA000019 Genomic DNA. Translation: BAB74027.1.
PIRAJAIQ. A01192.
AI2096.
RefSeqNP_486368.1. NC_003272.1.

3D structure databases

ProteinModelPortalP00964.
SMRP00964. Positions 2-474.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING103690.alr2328.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB74027; BAB74027; BAB74027.
GeneID1105925.
KEGGana:alr2328.
PATRIC22774830. VBINosSp37423_2882.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0174.
HOGENOMHOG000005157.
KOK01915.
OMAYESGMNS.
OrthoDBEOG6B360N.

Family and domain databases

Gene3D3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
TIGRFAMsTIGR00653. GlnA. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA_NOSS1
AccessionPrimary (citable) accession number: P00964
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families