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P00964

- GLNA_NOSS1

UniProt

P00964 - GLNA_NOSS1

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Protein

Glutamine synthetase

Gene

glnA

Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Enzyme regulationi

The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive (By similarity).By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. heterocyst differentiation Source: UniProtKB-KW
  3. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase
Gene namesi
Name:glnA
Ordered Locus Names:alr2328
OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
Taxonomic identifieri103690 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000002483: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 474473Glutamine synthetasePRO_0000153215Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei402 – 4021O-AMP-tyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Keywords - Developmental stagei

Heterocyst

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.

Protein-protein interaction databases

STRINGi103690.alr2328.

Structurei

3D structure databases

ProteinModelPortaliP00964.
SMRiP00964. Positions 2-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000005157.
KOiK01915.
OMAiYESGMNS.
OrthoDBiEOG6B360N.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00964-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTPQEVLKR IQDEKIELID LKFIDTVGTW QHLTLYQNQI DESSFSDGVP
60 70 80 90 100
FDGSSIRGWK AINESDMTMV LDPNTAWIDP FMEVPTLSIV CSIKEPRTGE
110 120 130 140 150
WYNRCPRVIA QKAIDYLVST GIGDTAFFGP EAEFFIFDSA RFAQNANEGY
160 170 180 190 200
YFLDSVEGAW NSGKEGTADK PNLAYKPRFK EGYFPVSPTD SFQDIRTEML
210 220 230 240 250
LTMAKLGVPI EKHHHEVATG GQCELGFRFG KLIEAADWLM IYKYVIKNVA
260 270 280 290 300
KKYGKTVTFM PKPIFGDNGS GMHCHQSIWK DGKPLFAGDQ YAGLSEMGLY
310 320 330 340 350
YIGGLLKHAP ALLAITNPST NSYKRLVPGY EAPVNLAYSQ GNRSASIRIP
360 370 380 390 400
LSGTNPKAKR LEFRCPDATS NPYLAFAAML CAGIDGIKNK IHPGEPLDKN
410 420 430 440 450
IYELSPEELA KVPSTPGSLE LALEALENDH AFLTDTGVFT EDFIQNWIDY
460 470
KLANEVKQMQ LRPHPYEFSI YYDV
Length:474
Mass (Da):53,136
Last modified:January 23, 2007 - v3
Checksum:i6A801A1B55262B28
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti107 – 1071R → P in CAA24982. 1 PublicationCurated
Sequence conflicti177 – 1771Missing AA sequence 1 PublicationCurated

Mass spectrometryi

Molecular mass is 53314 Da from positions 2 - 474. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00147 Genomic DNA. Translation: CAA24982.1.
BA000019 Genomic DNA. Translation: BAB74027.1.
PIRiA01192. AJAIQ.
AI2096.
RefSeqiNP_486368.1. NC_003272.1.
WP_010996484.1. NC_003272.1.

Genome annotation databases

EnsemblBacteriaiBAB74027; BAB74027; BAB74027.
GeneIDi1105925.
KEGGiana:alr2328.
PATRICi22774830. VBINosSp37423_2882.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00147 Genomic DNA. Translation: CAA24982.1 .
BA000019 Genomic DNA. Translation: BAB74027.1 .
PIRi A01192. AJAIQ.
AI2096.
RefSeqi NP_486368.1. NC_003272.1.
WP_010996484.1. NC_003272.1.

3D structure databases

ProteinModelPortali P00964.
SMRi P00964. Positions 2-474.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 103690.alr2328.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB74027 ; BAB74027 ; BAB74027 .
GeneIDi 1105925.
KEGGi ana:alr2328.
PATRICi 22774830. VBINosSp37423_2882.

Phylogenomic databases

eggNOGi COG0174.
HOGENOMi HOG000005157.
KOi K01915.
OMAi YESGMNS.
OrthoDBi EOG6B360N.

Family and domain databases

Gene3Di 3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
TIGRFAMsi TIGR00653. GlnA. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Different promoters for the Anabaena glutamine synthetase gene during growth usine molecular or fixed nitrogen."
    Tumer N.E., Robinson S.J., Haselkorn R.
    Nature 306:337-342(1983)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7120 / UTEX 2576.
  3. Singh H., Rajaram H., Apte S.K.
    Submitted (DEC-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 23-57; 98-104; 142-196; 213-243; 326-343 AND 391-411, MASS SPECTROMETRY.

Entry informationi

Entry nameiGLNA_NOSS1
AccessioniPrimary (citable) accession number: P00964
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In Anabaena, it is present in ammonia-grown vegetative cells as well as in heterocysts (for nitrogen fixation).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3