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Protein

Glutamine synthetase

Gene

glnA

Organism
Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

The activity of this enzyme could be controlled by adenylation under conditions of abundant glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi131Magnesium 1By similarity1
Metal bindingi133Magnesium 2By similarity1
Binding sitei211ATPBy similarity1
Metal bindingi216Magnesium 2By similarity1
Metal bindingi224Magnesium 2By similarity1
Binding sitei269L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi273Magnesium 1; via pros nitrogenBy similarity1
Binding sitei277ATPBy similarity1
Binding sitei325L-glutamateBy similarity1
Binding sitei331L-glutamateBy similarity1
Binding sitei343ATPBy similarity1
Binding sitei343L-glutamateBy similarity1
Binding sitei348ATPBy similarity1
Binding sitei357ATPBy similarity1
Metal bindingi362Magnesium 1By similarity1
Binding sitei364L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi275 – 277ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processNitrogen fixation
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I betaBy similarity
Short name:
GSI betaBy similarity
Gene namesi
Name:glnABy similarity
Ordered Locus Names:alr2328
OrganismiNostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)
Taxonomic identifieri103690 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
Proteomesi
  • UP000002483 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532152 – 474Glutamine synthetaseAdd BLAST473

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei402O-AMP-tyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Expressioni

Keywords - Developmental stagei

Heterocyst

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

Protein-protein interaction databases

STRINGi103690.alr2328.

Structurei

3D structure databases

ProteinModelPortaliP00964.
SMRiP00964.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni268 – 269L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.By similarity

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.
HOGENOMiHOG000005157.
KOiK01915.
OMAiKVLNQVG.
OrthoDBiPOG091H02IE.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P00964-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPQEVLKR IQDEKIELID LKFIDTVGTW QHLTLYQNQI DESSFSDGVP
60 70 80 90 100
FDGSSIRGWK AINESDMTMV LDPNTAWIDP FMEVPTLSIV CSIKEPRTGE
110 120 130 140 150
WYNRCPRVIA QKAIDYLVST GIGDTAFFGP EAEFFIFDSA RFAQNANEGY
160 170 180 190 200
YFLDSVEGAW NSGKEGTADK PNLAYKPRFK EGYFPVSPTD SFQDIRTEML
210 220 230 240 250
LTMAKLGVPI EKHHHEVATG GQCELGFRFG KLIEAADWLM IYKYVIKNVA
260 270 280 290 300
KKYGKTVTFM PKPIFGDNGS GMHCHQSIWK DGKPLFAGDQ YAGLSEMGLY
310 320 330 340 350
YIGGLLKHAP ALLAITNPST NSYKRLVPGY EAPVNLAYSQ GNRSASIRIP
360 370 380 390 400
LSGTNPKAKR LEFRCPDATS NPYLAFAAML CAGIDGIKNK IHPGEPLDKN
410 420 430 440 450
IYELSPEELA KVPSTPGSLE LALEALENDH AFLTDTGVFT EDFIQNWIDY
460 470
KLANEVKQMQ LRPHPYEFSI YYDV
Length:474
Mass (Da):53,136
Last modified:January 23, 2007 - v3
Checksum:i6A801A1B55262B28
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti107R → P in CAA24982 (Ref. 1) Curated1
Sequence conflicti177Missing AA sequence (Ref. 3) Curated1

Mass spectrometryi

Molecular mass is 53314 Da from positions 2 - 474. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00147 Genomic DNA. Translation: CAA24982.1.
BA000019 Genomic DNA. Translation: BAB74027.1.
PIRiA01192. AJAIQ.
AI2096.
RefSeqiWP_010996484.1. NC_003272.1.

Genome annotation databases

EnsemblBacteriaiBAB74027; BAB74027; BAB74027.
KEGGiana:alr2328.
PATRICi22774830. VBINosSp37423_2882.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00147 Genomic DNA. Translation: CAA24982.1.
BA000019 Genomic DNA. Translation: BAB74027.1.
PIRiA01192. AJAIQ.
AI2096.
RefSeqiWP_010996484.1. NC_003272.1.

3D structure databases

ProteinModelPortaliP00964.
SMRiP00964.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi103690.alr2328.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB74027; BAB74027; BAB74027.
KEGGiana:alr2328.
PATRICi22774830. VBINosSp37423_2882.

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.
HOGENOMiHOG000005157.
KOiK01915.
OMAiKVLNQVG.
OrthoDBiPOG091H02IE.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLN1B_NOSS1
AccessioniPrimary (citable) accession number: P00964
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: March 15, 2017
This is version 114 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In Anabaena, it is present in ammonia-grown vegetative cells as well as in heterocysts (for nitrogen fixation).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.