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UniProtKB - P00962 (SYQ_ECOLI)

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Protein

Glutamine--tRNA ligase

Gene

glnS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei271ATPBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutamine-tRNA ligase activity Source: EcoCyc
Complete GO annotation...

GO - Biological processi

  • glutaminyl-tRNA aminoacylation Source: EcoCyc
Complete GO annotation...

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:GLNS-MONOMER.
MetaCyc:GLNS-MONOMER.
BRENDAi6.1.1.18. 2026.
6.1.1.24. 2026.
SABIO-RKiP00962.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--tRNA ligase (EC:6.1.1.18)
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name:
GlnRS
Gene namesi
Name:glnS
Ordered Locus Names:b0680, JW0666
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10390. glnS.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001958332 – 554Glutamine--tRNA ligaseAdd BLAST553

Proteomic databases

EPDiP00962.
PaxDbiP00962.
PRIDEiP00962.

2D gel databases

SWISS-2DPAGEiP00962.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4261207. 27 interactors.
DIPiDIP-9787N.
IntActiP00962. 5 interactors.
MINTiMINT-1227909.
STRINGi316385.ECDH10B_0745.

Chemistry databases

BindingDBiP00962.

Structurei

Secondary structure

1554
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi11 – 21Combined sources11
Beta strandi29 – 32Combined sources4
Beta strandi36 – 38Combined sources3
Helixi42 – 57Combined sources16
Beta strandi61 – 66Combined sources6
Helixi71 – 73Combined sources3
Helixi76 – 88Combined sources13
Beta strandi94 – 96Combined sources3
Helixi100 – 103Combined sources4
Helixi104 – 116Combined sources13
Beta strandi119 – 123Combined sources5
Helixi127 – 134Combined sources8
Beta strandi137 – 139Combined sources3
Turni145 – 148Combined sources4
Helixi151 – 162Combined sources12
Beta strandi172 – 175Combined sources4
Helixi184 – 186Combined sources3
Beta strandi190 – 194Combined sources5
Turni200 – 202Combined sources3
Beta strandi207 – 210Combined sources4
Helixi212 – 222Combined sources11
Beta strandi226 – 231Combined sources6
Helixi232 – 234Combined sources3
Turni235 – 237Combined sources3
Helixi238 – 246Combined sources9
Beta strandi255 – 259Combined sources5
Beta strandi264 – 267Combined sources4
Helixi271 – 279Combined sources9
Beta strandi282 – 284Combined sources3
Beta strandi291 – 293Combined sources3
Helixi294 – 300Combined sources7
Helixi304 – 314Combined sources11
Helixi325 – 339Combined sources15
Beta strandi345 – 354Combined sources10
Beta strandi362 – 368Combined sources7
Helixi373 – 375Combined sources3
Beta strandi377 – 382Combined sources6
Beta strandi384 – 389Combined sources6
Turni390 – 392Combined sources3
Beta strandi393 – 396Combined sources4
Beta strandi403 – 405Combined sources3
Beta strandi408 – 412Combined sources5
Beta strandi417 – 424Combined sources8
Beta strandi427 – 429Combined sources3
Beta strandi433 – 436Combined sources4
Beta strandi460 – 462Combined sources3
Beta strandi466 – 473Combined sources8
Beta strandi476 – 480Combined sources5
Helixi482 – 484Combined sources3
Beta strandi485 – 487Combined sources3
Helixi488 – 491Combined sources4
Beta strandi496 – 504Combined sources9
Helixi506 – 510Combined sources5
Beta strandi516 – 519Combined sources4
Turni520 – 522Combined sources3
Beta strandi523 – 527Combined sources5
Turni529 – 531Combined sources3
Beta strandi534 – 536Combined sources3
Beta strandi538 – 544Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EUQX-ray3.10A1-548[»]
1EUYX-ray2.60A1-548[»]
1EXDX-ray2.70A1-548[»]
1GSGX-ray2.80P2-554[»]
1GTRX-ray2.50A2-554[»]
1GTSX-ray2.80A2-554[»]
1NYLX-ray2.60A9-547[»]
1O0BX-ray2.70A2-554[»]
1O0CX-ray2.70A2-554[»]
1QRSX-ray2.60A2-554[»]
1QRTX-ray2.70A2-554[»]
1QRUX-ray3.00A2-554[»]
1QTQX-ray2.25A2-554[»]
1ZJWX-ray2.50A2-554[»]
2RD2X-ray2.60A1-548[»]
2RE8X-ray2.60A1-548[»]
4JXXX-ray2.30A2-554[»]
4JXZX-ray2.40A2-554[»]
4JYZX-ray2.50A2-554[»]
ProteinModelPortaliP00962.
SMRiP00962.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00962.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi34 – 44"HIGH" regionAdd BLAST11
Motifi268 – 272"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105CX6. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000259232.
InParanoidiP00962.
KOiK01886.
PhylomeDBiP00962.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 3 hits.
HAMAPiMF_00126. Gln_tRNA_synth. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00962-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEAEARPTN FIRQIIDEDL ASGKHTTVHT RFPPEPNGYL HIGHAKSICL 
        60         70         80         90        100
NFGIAQDYKG QCNLRFDDTN PVKEDIEYVE SIKNDVEWLG FHWSGNVRYS 
       110        120        130        140        150
SDYFDQLHAY AIELINKGLA YVDELTPEQI REYRGTLTQP GKNSPYRDRS 
       160        170        180        190        200
VEENLALFEK MRAGGFEEGK ACLRAKIDMA SPFIVMRDPV LYRIKFAEHH 
       210        220        230        240        250
QTGNKWCIYP MYDFTHCISD ALEGITHSLC TLEFQDNRRL YDWVLDNITI 
       260        270        280        290        300
PVHPRQYEFS RLNLEYTVMS KRKLNLLVTD KHVEGWDDPR MPTISGLRRR 
       310        320        330        340        350
GYTAASIREF CKRIGVTKQD NTIEMASLES CIREDLNENA PRAMAVIDPV 
       360        370        380        390        400
KLVIENYQGE GEMVTMPNHP NKPEMGSRQV PFSGEIWIDR ADFREEANKQ 
       410        420        430        440        450
YKRLVLGKEV RLRNAYVIKA ERVEKDAEGN ITTIFCTYDA DTLSKDPADG 
       460        470        480        490        500
RKVKGVIHWV SAAHALPVEI RLYDRLFSVP NPGAADDFLS VINPESLVIK 
       510        520        530        540        550
QGFAEPSLKD AVAGKAFQFE REGYFCLDSR HSTAEKPVFN RTVGLRDTWA 

KVGE
Length:554
Mass (Da):63,478
Last modified:January 23, 2007 - v3
Checksum:iE720164EF990F335
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti549 – 554WAKVGE → GRK (PubMed:6288695).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01575 Genomic DNA. Translation: CAA24894.1.
U00096 Genomic DNA. Translation: AAC73774.1.
AP009048 Genomic DNA. Translation: BAA35328.1.
M16470, M16368 Genomic DNA. Translation: AAA69006.1.
PIRiG64802. SYECQT.
I41235.
RefSeqiNP_415206.1. NC_000913.3.
WP_001287154.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73774; AAC73774; b0680.
BAA35328; BAA35328; BAA35328.
GeneIDi945310.
KEGGiecj:JW0666.
eco:b0680.
PATRICifig|1411691.4.peg.1596.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSYQ_ECOLI
AccessioniPrimary (citable) accession number: P00962
Secondary accession number(s): Q59403
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: July 5, 2017
This is version 181 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families