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Protein

Glycine--tRNA ligase beta subunit

Gene

glyS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly).

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:GLYS-MONOMER.
MetaCyc:GLYS-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine--tRNA ligase beta subunit (EC:6.1.1.14)
Alternative name(s):
Glycyl-tRNA synthetase beta subunit
Short name:
GlyRS
Gene namesi
Name:glyS
Synonyms:glyS(B)
Ordered Locus Names:b3559, JW3530
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10410. glyS.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000728992 – 689Glycine--tRNA ligase beta subunitAdd BLAST688

Proteomic databases

PaxDbiP00961.
PRIDEiP00961.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.

Binary interactionsi

WithEntry#Exp.IntActNotes
glyQP009603EBI-551400,EBI-551191

Protein-protein interaction databases

DIPiDIP-6880N.
IntActiP00961. 11 interactors.
MINTiMINT-1276197.
STRINGi316385.ECDH10B_3739.

Structurei

3D structure databases

ProteinModelPortaliP00961.
SMRiP00961.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C38. Bacteria.
COG0751. LUCA.
HOGENOMiHOG000264302.
InParanoidiP00961.
KOiK01879.
PhylomeDBiP00961.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
HAMAPiMF_00255. Gly_tRNA_synth_beta. 1 hit.
InterProiView protein in InterPro
IPR008909. DALR_anticod-bd.
IPR015944. Gly-tRNA-synth_bsu.
IPR006194. Gly-tRNA-synth_heterodimer.
IPR009080. tRNAsynth_Ia_anticodon-bd.
PANTHERiPTHR30075. PTHR30075. 1 hit.
PfamiView protein in Pfam
PF05746. DALR_1. 1 hit.
PF02092. tRNA_synt_2f. 1 hit.
PRINTSiPR01045. TRNASYNTHGB.
TIGRFAMsiTIGR00211. glyS. 1 hit.
PROSITEiView protein in PROSITE
PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00961-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKTFLVEI GTEELPPKAL RSLAESFAAN FTAELDNAGL AHGTVQWFAA
60 70 80 90 100
PRRLALKVAN LAEAQPDREI EKRGPAIAQA FDAEGKPSKA AEGWARGCGI
110 120 130 140 150
TVDQAERLTT DKGEWLLYRA HVKGESTEAL LPNMVATSLA KLPIPKLMRW
160 170 180 190 200
GASDVHFVRP VHTVTLLLGD KVIPATILGI QSDRVIRGHR FMGEPEFTID
210 220 230 240 250
NADQYPEILR ERGKVIADYE ERKAKIKADA EEAARKIGGN ADLSESLLEE
260 270 280 290 300
VASLVEWPVV LTAKFEEKFL AVPAEALVYT MKGDQKYFPV YANDGKLLPN
310 320 330 340 350
FIFVANIESK DPQQIISGNE KVVRPRLADA EFFFNTDRKK RLEDNLPRLQ
360 370 380 390 400
TVLFQQQLGT LRDKTDRIQA LAGWIAEQIG ADVNHATRAG LLSKCDLMTN
410 420 430 440 450
MVFEFTDTQG VMGMHYARHD GEAEDVAVAL NEQYQPRFAG DDLPSNPVAC
460 470 480 490 500
ALAIADKMDT LAGIFGIGQH PKGDKDPFAL RRAALGVLRI IVEKNLNLDL
510 520 530 540 550
QTLTEEAVRL YGDKLTNANV VDDVIDFMLG RFRAWYQDEG YTVDTIQAVL
560 570 580 590 600
ARRPTRPADF DARMKAVSHF RTLDAAAALA AANKRVSNIL AKSDEVLSDR
610 620 630 640 650
VNASTLKEPE EIKLAMQVVV LRDKLEPYFT EGRYQDALVE LAELREPVDA
660 670 680
FFDKVMVMVD DKELRINRLT MLEKLRELFL RVADISLLQ
Length:689
Mass (Da):76,813
Last modified:January 23, 2007 - v4
Checksum:iF123D53B25BAE23A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2S → Q in AAA23915 (PubMed:6309809).Curated1
Sequence conflicti91A → P in AAA23915 (PubMed:6309809).Curated1
Sequence conflicti154 – 156DVH → AAA in AAA23915 (PubMed:6309809).Curated3
Sequence conflicti211 – 212ER → DG in AAA23915 (PubMed:6309809).Curated2
Sequence conflicti342L → D in AAA23915 (PubMed:6309809).Curated1
Sequence conflicti361L → V in AAA23915 (PubMed:6309809).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01622 Genomic DNA. Translation: AAA23915.1.
U00039 Genomic DNA. Translation: AAB18536.1.
U00096 Genomic DNA. Translation: AAC76583.1.
AP009048 Genomic DNA. Translation: BAE77735.1.
J01623 Genomic DNA. Translation: AAA23917.1.
PIRiS47780. SYECGB.
RefSeqiNP_418016.1. NC_000913.3.
WP_001291772.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76583; AAC76583; b3559.
BAE77735; BAE77735; BAE77735.
GeneIDi948080.
KEGGiecj:JW3530.
eco:b3559.
PATRICifig|1411691.4.peg.3154.

Similar proteinsi

Entry informationi

Entry nameiSYGB_ECOLI
AccessioniPrimary (citable) accession number: P00961
Secondary accession number(s): Q2M7M1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 159 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. SIMILARITY comments
    Index of protein domains and families