P00961 (SYGB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase beta subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase beta subunit
Short name=GlyRS

Gene names

Name:	glyS
Synonyms:	glyS(B)
Ordered Locus Names:	b3559, JW3530

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00255
Subunit structure	Tetramer of two alpha and two beta subunits.
Subcellular location	Cytoplasm HAMAP MF_00255.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arginine-tRNA ligase activity Inferred from electronic annotation. Source: InterPro glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 689	688	Glycine--tRNA ligase beta subunit HAMAP MF_00255		PRO_0000072899
Experimental info
	Sequence conflict	2	1	S → Q in AAA23915. Ref.1
	Sequence conflict	91	1	A → P in AAA23915. Ref.1
	Sequence conflict	154 – 156	3	DVH → AAA in AAA23915. Ref.1
	Sequence conflict	211 – 212	2	ER → DG in AAA23915. Ref.1
	Sequence conflict	342	1	L → D in AAA23915. Ref.1
	Sequence conflict	361	1	L → V in AAA23915. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P00961 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: F123D53B25BAE23A	FASTA	689	76,813
	10 20 30 40 50 60 MSEKTFLVEI GTEELPPKAL RSLAESFAAN FTAELDNAGL AHGTVQWFAA PRRLALKVAN 70 80 90 100 110 120 LAEAQPDREI EKRGPAIAQA FDAEGKPSKA AEGWARGCGI TVDQAERLTT DKGEWLLYRA 130 140 150 160 170 180 HVKGESTEAL LPNMVATSLA KLPIPKLMRW GASDVHFVRP VHTVTLLLGD KVIPATILGI 190 200 210 220 230 240 QSDRVIRGHR FMGEPEFTID NADQYPEILR ERGKVIADYE ERKAKIKADA EEAARKIGGN 250 260 270 280 290 300 ADLSESLLEE VASLVEWPVV LTAKFEEKFL AVPAEALVYT MKGDQKYFPV YANDGKLLPN 310 320 330 340 350 360 FIFVANIESK DPQQIISGNE KVVRPRLADA EFFFNTDRKK RLEDNLPRLQ TVLFQQQLGT 370 380 390 400 410 420 LRDKTDRIQA LAGWIAEQIG ADVNHATRAG LLSKCDLMTN MVFEFTDTQG VMGMHYARHD 430 440 450 460 470 480 GEAEDVAVAL NEQYQPRFAG DDLPSNPVAC ALAIADKMDT LAGIFGIGQH PKGDKDPFAL 490 500 510 520 530 540 RRAALGVLRI IVEKNLNLDL QTLTEEAVRL YGDKLTNANV VDDVIDFMLG RFRAWYQDEG 550 560 570 580 590 600 YTVDTIQAVL ARRPTRPADF DARMKAVSHF RTLDAAAALA AANKRVSNIL AKSDEVLSDR 610 620 630 640 650 660 VNASTLKEPE EIKLAMQVVV LRDKLEPYFT EGRYQDALVE LAELREPVDA FFDKVMVMVD 670 680 DKELRINRLT MLEKLRELFL RVADISLLQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structures of both subunits of Escherichia coli glycyl-tRNA synthetase." Webster T.A., Gibson B.W., Keng T., Biemann K., Schimmel P. J. Biol. Chem. 258:10637-10641(1983) [PubMed: 6309809] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Gene for Escherichia coli glycyl-tRNA synthetase has tandem subunit coding regions in the same reading frame." Keng T., Webster T.A., Sauer R.T., Schimmel P. J. Biol. Chem. 257:12503-12508(1982) [PubMed: 6290471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20.
[6]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-13. Strain: K12 / EMG2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J01622 Genomic DNA. Translation: AAA23915.1. U00039 Genomic DNA. Translation: AAB18536.1. U00096 Genomic DNA. Translation: AAC76583.1. AP009048 Genomic DNA. Translation: BAE77735.1. J01623 Genomic DNA. Translation: AAA23917.1.
PIR	SYECGB. S47780.
RefSeq	NP_418016.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P00961.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6880N.
IntAct	P00961. 12 interactions.
MINT	MINT-1276197.
PTM databases
PhosSite	P00961.
2D gel databases
ECO2DBASE	E077.5. 6TH EDITION.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000360; EBESCP00000000360; EBESCG00000000294. EBESCT00000015779; EBESCP00000015070; EBESCG00000014839.
GeneID	948080.
GenomeReviews	Gene locus JW3530 in contig AP009048_GR. Gene locus b3559 in contig U00096_GR.
KEGG	ecj:JW3530. eco:b3559.
PATRIC	32122592. VBIEscCol129921_3673.
Organism-specific databases
EchoBASE	EB0405.
EcoGene	EG10410. glyS.
Phylogenomic databases
eggNOG	COG0751.
GeneTree	EBGT00050000011475.
HOGENOM	HBG586599.
OMA	FIRPVHT.
PhylomeDB	P00961.
ProtClustDB	PRK01233.
Enzyme and pathway databases
BioCyc	EcoCyc:GLYS-MONOMER. MetaCyc:GLYS-MONOMER.
Gene expression databases
Genevestigator	P00961.
Family and domain databases
HAMAP	MF_00255. Gly_tRNA_synth_beta. [Tree]
InterPro	IPR008909. DALR_anticod-bd. IPR006194. Gly-tRNA-synth_II_heterodimer. IPR015944. Gly-tRNA-synth_IIc_bsu. [Graphical view]
KO	K01879.
Pfam	PF05746. DALR_1. 1 hit. PF02092. tRNA_synt_2f. 1 hit. [Graphical view]
PRINTS	PR01045. TRNASYNTHGB.
SMART	SM00836. DALR_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00211. GlyS. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGB_ECOLI

Accession

Primary (citable) accession number: P00961
Secondary accession number(s): Q2M7M1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 116 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program