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Protein

Glycine--tRNA ligase beta subunit

Gene

glyS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:GLYS-MONOMER.
ECOL316407:JW3530-MONOMER.
MetaCyc:GLYS-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine--tRNA ligase beta subunit (EC:6.1.1.14)
Alternative name(s):
Glycyl-tRNA synthetase beta subunit
Short name:
GlyRS
Gene namesi
Name:glyS
Synonyms:glyS(B)
Ordered Locus Names:b3559, JW3530
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10410. glyS.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 689688Glycine--tRNA ligase beta subunitPRO_0000072899Add
BLAST

Proteomic databases

EPDiP00961.
PaxDbiP00961.
PRIDEiP00961.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.

Binary interactionsi

WithEntry#Exp.IntActNotes
glyQP009603EBI-551400,EBI-551191

Protein-protein interaction databases

DIPiDIP-6880N.
IntActiP00961. 11 interactions.
MINTiMINT-1276197.
STRINGi511145.b3559.

Structurei

3D structure databases

ProteinModelPortaliP00961.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C38. Bacteria.
COG0751. LUCA.
HOGENOMiHOG000264302.
InParanoidiP00961.
KOiK01879.
OMAiLPIPKRM.
OrthoDBiEOG661H9S.
PhylomeDBiP00961.

Family and domain databases

HAMAPiMF_00255. Gly_tRNA_synth_beta.
InterProiIPR008909. DALR_anticod-bd.
IPR015944. Gly-tRNA-synth_bsu.
IPR006194. Gly-tRNA-synth_heterodimer.
[Graphical view]
PfamiPF05746. DALR_1. 1 hit.
PF02092. tRNA_synt_2f. 1 hit.
[Graphical view]
PRINTSiPR01045. TRNASYNTHGB.
TIGRFAMsiTIGR00211. glyS. 1 hit.
PROSITEiPS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00961-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKTFLVEI GTEELPPKAL RSLAESFAAN FTAELDNAGL AHGTVQWFAA
60 70 80 90 100
PRRLALKVAN LAEAQPDREI EKRGPAIAQA FDAEGKPSKA AEGWARGCGI
110 120 130 140 150
TVDQAERLTT DKGEWLLYRA HVKGESTEAL LPNMVATSLA KLPIPKLMRW
160 170 180 190 200
GASDVHFVRP VHTVTLLLGD KVIPATILGI QSDRVIRGHR FMGEPEFTID
210 220 230 240 250
NADQYPEILR ERGKVIADYE ERKAKIKADA EEAARKIGGN ADLSESLLEE
260 270 280 290 300
VASLVEWPVV LTAKFEEKFL AVPAEALVYT MKGDQKYFPV YANDGKLLPN
310 320 330 340 350
FIFVANIESK DPQQIISGNE KVVRPRLADA EFFFNTDRKK RLEDNLPRLQ
360 370 380 390 400
TVLFQQQLGT LRDKTDRIQA LAGWIAEQIG ADVNHATRAG LLSKCDLMTN
410 420 430 440 450
MVFEFTDTQG VMGMHYARHD GEAEDVAVAL NEQYQPRFAG DDLPSNPVAC
460 470 480 490 500
ALAIADKMDT LAGIFGIGQH PKGDKDPFAL RRAALGVLRI IVEKNLNLDL
510 520 530 540 550
QTLTEEAVRL YGDKLTNANV VDDVIDFMLG RFRAWYQDEG YTVDTIQAVL
560 570 580 590 600
ARRPTRPADF DARMKAVSHF RTLDAAAALA AANKRVSNIL AKSDEVLSDR
610 620 630 640 650
VNASTLKEPE EIKLAMQVVV LRDKLEPYFT EGRYQDALVE LAELREPVDA
660 670 680
FFDKVMVMVD DKELRINRLT MLEKLRELFL RVADISLLQ
Length:689
Mass (Da):76,813
Last modified:January 23, 2007 - v4
Checksum:iF123D53B25BAE23A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21S → Q in AAA23915 (PubMed:6309809).Curated
Sequence conflicti91 – 911A → P in AAA23915 (PubMed:6309809).Curated
Sequence conflicti154 – 1563DVH → AAA in AAA23915 (PubMed:6309809).Curated
Sequence conflicti211 – 2122ER → DG in AAA23915 (PubMed:6309809).Curated
Sequence conflicti342 – 3421L → D in AAA23915 (PubMed:6309809).Curated
Sequence conflicti361 – 3611L → V in AAA23915 (PubMed:6309809).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01622 Genomic DNA. Translation: AAA23915.1.
U00039 Genomic DNA. Translation: AAB18536.1.
U00096 Genomic DNA. Translation: AAC76583.1.
AP009048 Genomic DNA. Translation: BAE77735.1.
J01623 Genomic DNA. Translation: AAA23917.1.
PIRiS47780. SYECGB.
RefSeqiNP_418016.1. NC_000913.3.
WP_001291772.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76583; AAC76583; b3559.
BAE77735; BAE77735; BAE77735.
GeneIDi948080.
KEGGiecj:JW3530.
eco:b3559.
PATRICi32122592. VBIEscCol129921_3673.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01622 Genomic DNA. Translation: AAA23915.1.
U00039 Genomic DNA. Translation: AAB18536.1.
U00096 Genomic DNA. Translation: AAC76583.1.
AP009048 Genomic DNA. Translation: BAE77735.1.
J01623 Genomic DNA. Translation: AAA23917.1.
PIRiS47780. SYECGB.
RefSeqiNP_418016.1. NC_000913.3.
WP_001291772.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP00961.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6880N.
IntActiP00961. 11 interactions.
MINTiMINT-1276197.
STRINGi511145.b3559.

Proteomic databases

EPDiP00961.
PaxDbiP00961.
PRIDEiP00961.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76583; AAC76583; b3559.
BAE77735; BAE77735; BAE77735.
GeneIDi948080.
KEGGiecj:JW3530.
eco:b3559.
PATRICi32122592. VBIEscCol129921_3673.

Organism-specific databases

EchoBASEiEB0405.
EcoGeneiEG10410. glyS.

Phylogenomic databases

eggNOGiENOG4105C38. Bacteria.
COG0751. LUCA.
HOGENOMiHOG000264302.
InParanoidiP00961.
KOiK01879.
OMAiLPIPKRM.
OrthoDBiEOG661H9S.
PhylomeDBiP00961.

Enzyme and pathway databases

BioCyciEcoCyc:GLYS-MONOMER.
ECOL316407:JW3530-MONOMER.
MetaCyc:GLYS-MONOMER.

Miscellaneous databases

PROiP00961.

Family and domain databases

HAMAPiMF_00255. Gly_tRNA_synth_beta.
InterProiIPR008909. DALR_anticod-bd.
IPR015944. Gly-tRNA-synth_bsu.
IPR006194. Gly-tRNA-synth_heterodimer.
[Graphical view]
PfamiPF05746. DALR_1. 1 hit.
PF02092. tRNA_synt_2f. 1 hit.
[Graphical view]
PRINTSiPR01045. TRNASYNTHGB.
TIGRFAMsiTIGR00211. glyS. 1 hit.
PROSITEiPS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structures of both subunits of Escherichia coli glycyl-tRNA synthetase."
    Webster T.A., Gibson B.W., Keng T., Biemann K., Schimmel P.
    J. Biol. Chem. 258:10637-10641(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Gene for Escherichia coli glycyl-tRNA synthetase has tandem subunit coding regions in the same reading frame."
    Keng T., Webster T.A., Sauer R.T., Schimmel P.
    J. Biol. Chem. 257:12503-12508(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20.
  6. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-13.
    Strain: K12 / EMG2.
  7. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.

Entry informationi

Entry nameiSYGB_ECOLI
AccessioniPrimary (citable) accession number: P00961
Secondary accession number(s): Q2M7M1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 150 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.