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Protein

Glycine--tRNA ligase alpha subunit

Gene

glyQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly).

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:GLYQ-MONOMER
MetaCyc:GLYQ-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine--tRNA ligase alpha subunit (EC:6.1.1.14)
Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name:
GlyRS
Gene namesi
Name:glyQ
Synonyms:glyS(A)
Ordered Locus Names:b3560, JW3531
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG10409 glyQ

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000728361 – 303Glycine--tRNA ligase alpha subunitAdd BLAST303

Proteomic databases

EPDiP00960
PaxDbiP00960
PRIDEiP00960

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.

Binary interactionsi

WithEntry#Exp.IntActNotes
glySP009613EBI-551191,EBI-551400

Protein-protein interaction databases

BioGridi4259717, 48 interactors
DIPiDIP-9816N
IntActiP00960, 33 interactors
STRINGi316407.85676484

Structurei

3D structure databases

ProteinModelPortaliP00960
SMRiP00960
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0752 LUCA
HOGENOMiHOG000264291
InParanoidiP00960
KOiK01878
OMAiLGSYYQF
PhylomeDBiP00960

Family and domain databases

CDDicd00733 GlyRS_alpha_core, 1 hit
HAMAPiMF_00254 Gly_tRNA_synth_alpha, 1 hit
InterProiView protein in InterPro
IPR006194 Gly-tRNA-synth_heterodimer
IPR002310 Gly-tRNA_ligase_asu
PANTHERiPTHR44629 PTHR44629, 1 hit
PfamiView protein in Pfam
PF02091 tRNA-synt_2e, 1 hit
PRINTSiPR01044 TRNASYNTHGA
TIGRFAMsiTIGR00388 glyQ, 1 hit
PROSITEiView protein in PROSITE
PS50861 AA_TRNA_LIGASE_II_GLYAB, 1 hit

Sequencei

Sequence statusi: Complete.

P00960-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKFDTRTFQ GLILTLQDYW ARQGCTIVQP LDMEVGAGTS HPMTCLRELG
60 70 80 90 100
PEPMAAAYVQ PSRRPTDGRY GENPNRLQHY YQFQVVIKPS PDNIQELYLG
110 120 130 140 150
SLKELGMDPT IHDIRFVEDN WENPTLGAWG LGWEVWLNGM EVTQFTYFQQ
160 170 180 190 200
VGGLECKPVT GEITYGLERL AMYIQGVDSV YDLVWSDGPL GKTTYGDVFH
210 220 230 240 250
QNEVEQSTYN FEYADVDFLF TCFEQYEKEA QQLLALENPL PLPAYERILK
260 270 280 290 300
AAHSFNLLDA RKAISVTERQ RYILRIRTLT KAVAEAYYAS REALGFPMCN

KDK
Length:303
Mass (Da):34,774
Last modified:October 1, 1994 - v2
Checksum:i91E20623ADFD4DDB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48E → A in AAA23914 (PubMed:6309809).Curated1
Sequence conflicti65P → A in AAA23914 (PubMed:6309809).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01622 Genomic DNA Translation: AAA23914.1
U00039 Genomic DNA Translation: AAB18537.1
U00096 Genomic DNA Translation: AAC76584.1
AP009048 Genomic DNA Translation: BAE77734.1
J01623 Genomic DNA Translation: AAA23916.1
PIRiB65155 SYECGA
RefSeqiNP_418017.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAC76584; AAC76584; b3560
BAE77734; BAE77734; BAE77734
GeneIDi948079
KEGGiecj:JW3531
eco:b3560
PATRICifig|511145.12.peg.3674

Entry informationi

Entry nameiSYGA_ECOLI
AccessioniPrimary (citable) accession number: P00960
Secondary accession number(s): Q2M7M2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 1, 1994
Last modified: March 28, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. SIMILARITY comments
    Index of protein domains and families

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