Reviewed, UniProtKB/Swiss-Prot P00960 (SYGA_ECOLI)

Names and origin

Protein names

Recommended name:
    Glycyl-tRNA synthetase alpha subunit
    EC=6.1.1.14
Alternative name(s):
    Glycine--tRNA ligase alpha subunit
      Short name=GlyRS

Gene names

Name:	glyQ
Synonyms:	glyS(A)
Ordered Locus Names:	b3560, JW3531

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits. HAMAP MF_00254
Subcellular location	Cytoplasm. HAMAP MF_00254
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
glpQ	P09394	1	EBI-551191,EBI-1128141

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Glycyl-tRNA synthetase alpha subunit HAMAP MF_00254		PRO_0000072836
Experimental info
	Sequence conflict	48	1	E → A in AAA23914. Ref.1
	Sequence conflict	65	1	P → A in AAA23914. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P00960-1 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: 91E20623ADFD4DDB	FASTA	303	34,774
	10 20 30 40 50 60 MQKFDTRTFQ GLILTLQDYW ARQGCTIVQP LDMEVGAGTS HPMTCLRELG PEPMAAAYVQ 70 80 90 100 110 120 PSRRPTDGRY GENPNRLQHY YQFQVVIKPS PDNIQELYLG SLKELGMDPT IHDIRFVEDN 130 140 150 160 170 180 WENPTLGAWG LGWEVWLNGM EVTQFTYFQQ VGGLECKPVT GEITYGLERL AMYIQGVDSV 190 200 210 220 230 240 YDLVWSDGPL GKTTYGDVFH QNEVEQSTYN FEYADVDFLF TCFEQYEKEA QQLLALENPL 250 260 270 280 290 300 PLPAYERILK AAHSFNLLDA RKAISVTERQ RYILRIRTLT KAVAEAYYAS REALGFPMCN KDK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structures of both subunits of Escherichia coli glycyl-tRNA synthetase." Webster T.A., Gibson B.W., Keng T., Biemann K., Schimmel P. J. Biol. Chem. 258:10637-10641(1983) [PubMed: 6309809] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Gene for Escherichia coli glycyl-tRNA synthetase has tandem subunit coding regions in the same reading frame." Keng T., Webster T.A., Sauer R.T., Schimmel P. J. Biol. Chem. 257:12503-12508(1982) [PubMed: 6290471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 268-303.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J01622 Genomic DNA. Translation: AAA23914.1. U00039 Genomic DNA. Translation: AAB18537.1. U00096 Genomic DNA. Translation: AAC76584.1. AP009048 Genomic DNA. Translation: BAE77734.1. J01623 Genomic DNA. Translation: AAA23916.1.
PIR	SYECGA. B65155.
RefSeq	AP_004233.1. NP_418017.1.
3D structure databases
HSSP	HSSP built from PDB template 1J5W based on UniProtKB Q9WY59.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9816N.
IntAct	P00960. 23 interactions.
Genome annotation databases
GeneID	948079.
GenomeReviews	Gene locus JW3531 in contig AP009048_GR. Gene locus b3560 in contig U00096_GR.
KEGG	ecj:JW3531. eco:b3560.
Organism-specific databases
EchoBASE	EB0404.
EcoGene	EG10409. glyQ.
CMR	Search...
Phylogenomic databases
HOGENOM	P00960.
OMA	P00960. CRRPTDG.
Enzyme and pathway databases
BioCyc	EcoCyc:GLYQ-MON. MetaCyc:GLYQ-MON.
Family and domain databases
HAMAP	MF_00254. [Tree]
InterPro	IPR006194. Gly-tRNA-synth_II_heterodimer. IPR002310. Gly-tRNA_synth_IIc_asu. [Graphical view]
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
ProDom	PD006985. tRNA_synt_2e. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00388. glyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_ECOLI

Accession

Primary (citable) accession number: P00960
Secondary accession number(s): Q2M7M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)