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Protein

Glycine--tRNA ligase alpha subunit

Gene

glyQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:GLYQ-MONOMER.
ECOL316407:JW3531-MONOMER.
MetaCyc:GLYQ-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine--tRNA ligase alpha subunit (EC:6.1.1.14)
Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name:
GlyRS
Gene namesi
Name:glyQ
Synonyms:glyS(A)
Ordered Locus Names:b3560, JW3531
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10409. glyQ.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000728361 – 303Glycine--tRNA ligase alpha subunitAdd BLAST303

Proteomic databases

EPDiP00960.
PaxDbiP00960.
PRIDEiP00960.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.

Binary interactionsi

WithEntry#Exp.IntActNotes
glySP009613EBI-551191,EBI-551400

Protein-protein interaction databases

BioGridi4259717. 23 interactors.
DIPiDIP-9816N.
IntActiP00960. 32 interactors.
MINTiMINT-1225336.
STRINGi511145.b3560.

Structurei

3D structure databases

ProteinModelPortaliP00960.
SMRiP00960.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0752. LUCA.
HOGENOMiHOG000264291.
InParanoidiP00960.
KOiK01878.
OMAiLGSYYQF.
PhylomeDBiP00960.

Family and domain databases

CDDicd00733. GlyRS_alpha_core. 1 hit.
HAMAPiMF_00254. Gly_tRNA_synth_alpha. 1 hit.
InterProiIPR006194. Gly-tRNA-synth_heterodimer.
IPR002310. Gly-tRNA_ligase_asu.
[Graphical view]
PfamiPF02091. tRNA-synt_2e. 1 hit.
[Graphical view]
PRINTSiPR01044. TRNASYNTHGA.
TIGRFAMsiTIGR00388. glyQ. 1 hit.
PROSITEiPS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00960-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKFDTRTFQ GLILTLQDYW ARQGCTIVQP LDMEVGAGTS HPMTCLRELG
60 70 80 90 100
PEPMAAAYVQ PSRRPTDGRY GENPNRLQHY YQFQVVIKPS PDNIQELYLG
110 120 130 140 150
SLKELGMDPT IHDIRFVEDN WENPTLGAWG LGWEVWLNGM EVTQFTYFQQ
160 170 180 190 200
VGGLECKPVT GEITYGLERL AMYIQGVDSV YDLVWSDGPL GKTTYGDVFH
210 220 230 240 250
QNEVEQSTYN FEYADVDFLF TCFEQYEKEA QQLLALENPL PLPAYERILK
260 270 280 290 300
AAHSFNLLDA RKAISVTERQ RYILRIRTLT KAVAEAYYAS REALGFPMCN

KDK
Length:303
Mass (Da):34,774
Last modified:October 1, 1994 - v2
Checksum:i91E20623ADFD4DDB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48E → A in AAA23914 (PubMed:6309809).Curated1
Sequence conflicti65P → A in AAA23914 (PubMed:6309809).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01622 Genomic DNA. Translation: AAA23914.1.
U00039 Genomic DNA. Translation: AAB18537.1.
U00096 Genomic DNA. Translation: AAC76584.1.
AP009048 Genomic DNA. Translation: BAE77734.1.
J01623 Genomic DNA. Translation: AAA23916.1.
PIRiB65155. SYECGA.
RefSeqiNP_418017.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC76584; AAC76584; b3560.
BAE77734; BAE77734; BAE77734.
GeneIDi948079.
KEGGiecj:JW3531.
eco:b3560.
PATRICi32122594. VBIEscCol129921_3674.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01622 Genomic DNA. Translation: AAA23914.1.
U00039 Genomic DNA. Translation: AAB18537.1.
U00096 Genomic DNA. Translation: AAC76584.1.
AP009048 Genomic DNA. Translation: BAE77734.1.
J01623 Genomic DNA. Translation: AAA23916.1.
PIRiB65155. SYECGA.
RefSeqiNP_418017.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP00960.
SMRiP00960.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259717. 23 interactors.
DIPiDIP-9816N.
IntActiP00960. 32 interactors.
MINTiMINT-1225336.
STRINGi511145.b3560.

Proteomic databases

EPDiP00960.
PaxDbiP00960.
PRIDEiP00960.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76584; AAC76584; b3560.
BAE77734; BAE77734; BAE77734.
GeneIDi948079.
KEGGiecj:JW3531.
eco:b3560.
PATRICi32122594. VBIEscCol129921_3674.

Organism-specific databases

EchoBASEiEB0404.
EcoGeneiEG10409. glyQ.

Phylogenomic databases

eggNOGiCOG0752. LUCA.
HOGENOMiHOG000264291.
InParanoidiP00960.
KOiK01878.
OMAiLGSYYQF.
PhylomeDBiP00960.

Enzyme and pathway databases

BioCyciEcoCyc:GLYQ-MONOMER.
ECOL316407:JW3531-MONOMER.
MetaCyc:GLYQ-MONOMER.

Miscellaneous databases

PROiP00960.

Family and domain databases

CDDicd00733. GlyRS_alpha_core. 1 hit.
HAMAPiMF_00254. Gly_tRNA_synth_alpha. 1 hit.
InterProiIPR006194. Gly-tRNA-synth_heterodimer.
IPR002310. Gly-tRNA_ligase_asu.
[Graphical view]
PfamiPF02091. tRNA-synt_2e. 1 hit.
[Graphical view]
PRINTSiPR01044. TRNASYNTHGA.
TIGRFAMsiTIGR00388. glyQ. 1 hit.
PROSITEiPS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYGA_ECOLI
AccessioniPrimary (citable) accession number: P00960
Secondary accession number(s): Q2M7M2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.