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Protein

Methionine--tRNA ligase

Gene

metG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi146 – 1461Zinc
Metal bindingi149 – 1491Zinc
Metal bindingi159 – 1591Zinc
Metal bindingi162 – 1621Zinc
Binding sitei336 – 3361ATP

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • methionine-tRNA ligase activity Source: EcoCyc
  • tRNA binding Source: UniProtKB-HAMAP
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • methionyl-tRNA aminoacylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:METG-MONOMER.
ECOL316407:JW2101-MONOMER.
MetaCyc:METG-MONOMER.
BRENDAi6.1.1.10. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase (EC:6.1.1.10)
Alternative name(s):
Methionyl-tRNA synthetase
Short name:
MetRS
Gene namesi
Name:metG
Ordered Locus Names:b2114, JW2101
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10586. metG.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi336 – 3361K → Q, A, E or R: Loss of activity. 2 Publications

Chemistry

ChEMBLiCHEMBL3367.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 677676Methionine--tRNA ligasePRO_0000139129Add
BLAST

Proteomic databases

EPDiP00959.
PaxDbiP00959.
PRIDEiP00959.

2D gel databases

SWISS-2DPAGEP00959.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
rplBP604222EBI-909268,EBI-543515

Protein-protein interaction databases

DIPiDIP-10194N.
IntActiP00959. 3 interactions.
STRINGi511145.b2114.

Chemistry

BindingDBiP00959.

Structurei

Secondary structure

1
677
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 126Combined sources
Beta strandi16 – 194Combined sources
Helixi23 – 4119Combined sources
Beta strandi45 – 539Combined sources
Helixi57 – 6610Combined sources
Helixi70 – 8718Combined sources
Beta strandi93 – 1008Combined sources
Helixi101 – 11616Combined sources
Beta strandi120 – 13011Combined sources
Turni131 – 1344Combined sources
Helixi139 – 1413Combined sources
Beta strandi142 – 1454Combined sources
Turni147 – 1493Combined sources
Beta strandi152 – 1554Combined sources
Turni160 – 1623Combined sources
Helixi168 – 1703Combined sources
Beta strandi172 – 1765Combined sources
Turni177 – 1793Combined sources
Beta strandi184 – 19310Combined sources
Helixi195 – 1984Combined sources
Helixi199 – 2079Combined sources
Helixi213 – 22513Combined sources
Beta strandi233 – 2375Combined sources
Beta strandi249 – 2513Combined sources
Helixi253 – 27220Combined sources
Helixi277 – 2826Combined sources
Beta strandi288 – 2958Combined sources
Helixi296 – 2983Combined sources
Helixi299 – 3035Combined sources
Helixi305 – 3128Combined sources
Beta strandi319 – 3246Combined sources
Beta strandi327 – 3293Combined sources
Turni336 – 3394Combined sources
Helixi344 – 3507Combined sources
Helixi353 – 36311Combined sources
Beta strandi371 – 3733Combined sources
Helixi375 – 38511Combined sources
Helixi386 – 3905Combined sources
Helixi392 – 3954Combined sources
Helixi398 – 4047Combined sources
Helixi416 – 4249Combined sources
Helixi426 – 4349Combined sources
Helixi438 – 45922Combined sources
Helixi461 – 4644Combined sources
Helixi471 – 49222Combined sources
Turni493 – 4953Combined sources
Helixi497 – 50711Combined sources
Helixi514 – 5174Combined sources
Helixi537 – 54711Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F4LX-ray1.85A1-551[»]
1MEANMR-A139-164[»]
1MEDNMR-A139-164[»]
1P7PX-ray1.80A2-552[»]
1PFUX-ray1.91A2-552[»]
1PFVX-ray1.70A2-552[»]
1PFWX-ray1.78A2-552[»]
1PFYX-ray1.93A2-552[»]
1PG0X-ray1.90A2-552[»]
1PG2X-ray1.75A2-552[»]
1QQTX-ray2.03A2-552[»]
3H97X-ray1.70A1-548[»]
3H99X-ray1.40A1-548[»]
3H9BX-ray1.50A1-548[»]
3H9CX-ray1.40A2-548[»]
ProteinModelPortaliP00959.
SMRiP00959. Positions 5-548, 571-676.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00959.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini575 – 677103tRNA-bindingAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi15 – 2511"HIGH" regionAdd
BLAST
Motifi333 – 3375"KMSKS" region

Sequence similaritiesi

Contains 1 tRNA-binding domain.Curated

Phylogenomic databases

eggNOGiENOG4105CKH. Bacteria.
COG0073. LUCA.
COG0143. LUCA.
HOGENOMiHOG000200400.
InParanoidiP00959.
KOiK01874.
OMAiFWPAMLD.
PhylomeDBiP00959.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.20.28.20. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_bsu_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR029038. MetRS_Zn.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00959-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQVAKKILV TCALPYANGS IHLGHMLEHI QADVWVRYQR MRGHEVNFIC
60 70 80 90 100
ADDAHGTPIM LKAQQLGITP EQMIGEMSQE HQTDFAGFNI SYDNYHSTHS
110 120 130 140 150
EENRQLSELI YSRLKENGFI KNRTISQLYD PEKGMFLPDR FVKGTCPKCK
160 170 180 190 200
SPDQYGDNCE VCGATYSPTE LIEPKSVVSG ATPVMRDSEH FFFDLPSFSE
210 220 230 240 250
MLQAWTRSGA LQEQVANKMQ EWFESGLQQW DISRDAPYFG FEIPNAPGKY
260 270 280 290 300
FYVWLDAPIG YMGSFKNLCD KRGDSVSFDE YWKKDSTAEL YHFIGKDIVY
310 320 330 340 350
FHSLFWPAML EGSNFRKPSN LFVHGYVTVN GAKMSKSRGT FIKASTWLNH
360 370 380 390 400
FDADSLRYYY TAKLSSRIDD IDLNLEDFVQ RVNADIVNKV VNLASRNAGF
410 420 430 440 450
INKRFDGVLA SELADPQLYK TFTDAAEVIG EAWESREFGK AVREIMALAD
460 470 480 490 500
LANRYVDEQA PWVVAKQEGR DADLQAICSM GINLFRVLMT YLKPVLPKLT
510 520 530 540 550
ERAEAFLNTE LTWDGIQQPL LGHKVNPFKA LYNRIDMRQV EALVEASKEE
560 570 580 590 600
VKAAAAPVTG PLADDPIQET ITFDDFAKVD LRVALIENAE FVEGSDKLLR
610 620 630 640 650
LTLDLGGEKR NVFSGIRSAY PDPQALIGRH TIMVANLAPR KMRFGISEGM
660 670
VMAAGPGGKD IFLLSPDAGA KPGHQVK
Length:677
Mass (Da):76,255
Last modified:January 23, 2007 - v2
Checksum:i363F3324AFD3202C
GO

Sequence cautioni

The sequence AAA60526 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02671 Genomic DNA. Translation: AAA24161.1.
X55791 Genomic DNA. Translation: CAA39315.1.
U00007 Genomic DNA. Translation: AAA60526.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75175.1.
AP009048 Genomic DNA. Translation: BAE76592.1.
PIRiS14427. SYECMT.
RefSeqiNP_416617.1. NC_000913.3.
WP_001350533.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75175; AAC75175; b2114.
BAE76592; BAE76592; BAE76592.
GeneIDi946643.
KEGGiecj:JW2101.
eco:b2114.
PATRICi32119561. VBIEscCol129921_2191.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02671 Genomic DNA. Translation: AAA24161.1.
X55791 Genomic DNA. Translation: CAA39315.1.
U00007 Genomic DNA. Translation: AAA60526.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75175.1.
AP009048 Genomic DNA. Translation: BAE76592.1.
PIRiS14427. SYECMT.
RefSeqiNP_416617.1. NC_000913.3.
WP_001350533.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F4LX-ray1.85A1-551[»]
1MEANMR-A139-164[»]
1MEDNMR-A139-164[»]
1P7PX-ray1.80A2-552[»]
1PFUX-ray1.91A2-552[»]
1PFVX-ray1.70A2-552[»]
1PFWX-ray1.78A2-552[»]
1PFYX-ray1.93A2-552[»]
1PG0X-ray1.90A2-552[»]
1PG2X-ray1.75A2-552[»]
1QQTX-ray2.03A2-552[»]
3H97X-ray1.70A1-548[»]
3H99X-ray1.40A1-548[»]
3H9BX-ray1.50A1-548[»]
3H9CX-ray1.40A2-548[»]
ProteinModelPortaliP00959.
SMRiP00959. Positions 5-548, 571-676.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-10194N.
IntActiP00959. 3 interactions.
STRINGi511145.b2114.

Chemistry

BindingDBiP00959.
ChEMBLiCHEMBL3367.

2D gel databases

SWISS-2DPAGEP00959.

Proteomic databases

EPDiP00959.
PaxDbiP00959.
PRIDEiP00959.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75175; AAC75175; b2114.
BAE76592; BAE76592; BAE76592.
GeneIDi946643.
KEGGiecj:JW2101.
eco:b2114.
PATRICi32119561. VBIEscCol129921_2191.

Organism-specific databases

EchoBASEiEB0581.
EcoGeneiEG10586. metG.

Phylogenomic databases

eggNOGiENOG4105CKH. Bacteria.
COG0073. LUCA.
COG0143. LUCA.
HOGENOMiHOG000200400.
InParanoidiP00959.
KOiK01874.
OMAiFWPAMLD.
PhylomeDBiP00959.

Enzyme and pathway databases

BioCyciEcoCyc:METG-MONOMER.
ECOL316407:JW2101-MONOMER.
MetaCyc:METG-MONOMER.
BRENDAi6.1.1.10. 2026.

Miscellaneous databases

EvolutionaryTraceiP00959.
PROiP00959.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.20.28.20. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_bsu_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR029038. MetRS_Zn.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYM_ECOLI
AccessioniPrimary (citable) accession number: P00959
Secondary accession number(s): Q2MAW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 180 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.