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Protein

Methionine--tRNA ligase

Gene

metG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi146Zinc1
Metal bindingi149Zinc1
Metal bindingi159Zinc1
Metal bindingi162Zinc1
Binding sitei336ATP1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • methionine-tRNA ligase activity Source: EcoCyc
  • tRNA binding Source: UniProtKB-HAMAP
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • methionyl-tRNA aminoacylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:METG-MONOMER.
ECOL316407:JW2101-MONOMER.
MetaCyc:METG-MONOMER.
BRENDAi6.1.1.10. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase (EC:6.1.1.10)
Alternative name(s):
Methionyl-tRNA synthetase
Short name:
MetRS
Gene namesi
Name:metG
Ordered Locus Names:b2114, JW2101
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10586. metG.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi336K → Q, A, E or R: Loss of activity. 2 Publications1

Chemistry databases

ChEMBLiCHEMBL3367.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001391292 – 677Methionine--tRNA ligaseAdd BLAST676

Proteomic databases

EPDiP00959.
PaxDbiP00959.
PRIDEiP00959.

2D gel databases

SWISS-2DPAGEP00959.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
rplBP604222EBI-909268,EBI-543515

Protein-protein interaction databases

DIPiDIP-10194N.
IntActiP00959. 3 interactors.
STRINGi511145.b2114.

Chemistry databases

BindingDBiP00959.

Structurei

Secondary structure

1677
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 12Combined sources6
Beta strandi16 – 19Combined sources4
Helixi23 – 41Combined sources19
Beta strandi45 – 53Combined sources9
Helixi57 – 66Combined sources10
Helixi70 – 87Combined sources18
Beta strandi93 – 100Combined sources8
Helixi101 – 116Combined sources16
Beta strandi120 – 130Combined sources11
Turni131 – 134Combined sources4
Helixi139 – 141Combined sources3
Beta strandi142 – 145Combined sources4
Turni147 – 149Combined sources3
Beta strandi152 – 155Combined sources4
Turni160 – 162Combined sources3
Helixi168 – 170Combined sources3
Beta strandi172 – 176Combined sources5
Turni177 – 179Combined sources3
Beta strandi184 – 193Combined sources10
Helixi195 – 198Combined sources4
Helixi199 – 207Combined sources9
Helixi213 – 225Combined sources13
Beta strandi233 – 237Combined sources5
Beta strandi249 – 251Combined sources3
Helixi253 – 272Combined sources20
Helixi277 – 282Combined sources6
Beta strandi288 – 295Combined sources8
Helixi296 – 298Combined sources3
Helixi299 – 303Combined sources5
Helixi305 – 312Combined sources8
Beta strandi319 – 324Combined sources6
Beta strandi327 – 329Combined sources3
Turni336 – 339Combined sources4
Helixi344 – 350Combined sources7
Helixi353 – 363Combined sources11
Beta strandi371 – 373Combined sources3
Helixi375 – 385Combined sources11
Helixi386 – 390Combined sources5
Helixi392 – 395Combined sources4
Helixi398 – 404Combined sources7
Helixi416 – 424Combined sources9
Helixi426 – 434Combined sources9
Helixi438 – 459Combined sources22
Helixi461 – 464Combined sources4
Helixi471 – 492Combined sources22
Turni493 – 495Combined sources3
Helixi497 – 507Combined sources11
Helixi514 – 517Combined sources4
Helixi537 – 547Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F4LX-ray1.85A1-551[»]
1MEANMR-A139-164[»]
1MEDNMR-A139-164[»]
1P7PX-ray1.80A2-552[»]
1PFUX-ray1.91A2-552[»]
1PFVX-ray1.70A2-552[»]
1PFWX-ray1.78A2-552[»]
1PFYX-ray1.93A2-552[»]
1PG0X-ray1.90A2-552[»]
1PG2X-ray1.75A2-552[»]
1QQTX-ray2.03A2-552[»]
3H97X-ray1.70A1-548[»]
3H99X-ray1.40A1-548[»]
3H9BX-ray1.50A1-548[»]
3H9CX-ray1.40A2-548[»]
ProteinModelPortaliP00959.
SMRiP00959.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00959.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini575 – 677tRNA-bindingAdd BLAST103

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi15 – 25"HIGH" regionAdd BLAST11
Motifi333 – 337"KMSKS" region5

Sequence similaritiesi

Contains 1 tRNA-binding domain.Curated

Phylogenomic databases

eggNOGiENOG4105CKH. Bacteria.
COG0073. LUCA.
COG0143. LUCA.
HOGENOMiHOG000200400.
InParanoidiP00959.
KOiK01874.
OMAiFWPAMLD.
PhylomeDBiP00959.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
cd02800. tRNA_bind_EcMetRS_like. 1 hit.
Gene3Di1.10.730.10. 1 hit.
2.20.28.20. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_bsu_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR029038. MetRS_Zn.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00959-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQVAKKILV TCALPYANGS IHLGHMLEHI QADVWVRYQR MRGHEVNFIC
60 70 80 90 100
ADDAHGTPIM LKAQQLGITP EQMIGEMSQE HQTDFAGFNI SYDNYHSTHS
110 120 130 140 150
EENRQLSELI YSRLKENGFI KNRTISQLYD PEKGMFLPDR FVKGTCPKCK
160 170 180 190 200
SPDQYGDNCE VCGATYSPTE LIEPKSVVSG ATPVMRDSEH FFFDLPSFSE
210 220 230 240 250
MLQAWTRSGA LQEQVANKMQ EWFESGLQQW DISRDAPYFG FEIPNAPGKY
260 270 280 290 300
FYVWLDAPIG YMGSFKNLCD KRGDSVSFDE YWKKDSTAEL YHFIGKDIVY
310 320 330 340 350
FHSLFWPAML EGSNFRKPSN LFVHGYVTVN GAKMSKSRGT FIKASTWLNH
360 370 380 390 400
FDADSLRYYY TAKLSSRIDD IDLNLEDFVQ RVNADIVNKV VNLASRNAGF
410 420 430 440 450
INKRFDGVLA SELADPQLYK TFTDAAEVIG EAWESREFGK AVREIMALAD
460 470 480 490 500
LANRYVDEQA PWVVAKQEGR DADLQAICSM GINLFRVLMT YLKPVLPKLT
510 520 530 540 550
ERAEAFLNTE LTWDGIQQPL LGHKVNPFKA LYNRIDMRQV EALVEASKEE
560 570 580 590 600
VKAAAAPVTG PLADDPIQET ITFDDFAKVD LRVALIENAE FVEGSDKLLR
610 620 630 640 650
LTLDLGGEKR NVFSGIRSAY PDPQALIGRH TIMVANLAPR KMRFGISEGM
660 670
VMAAGPGGKD IFLLSPDAGA KPGHQVK
Length:677
Mass (Da):76,255
Last modified:January 23, 2007 - v2
Checksum:i363F3324AFD3202C
GO

Sequence cautioni

The sequence AAA60526 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02671 Genomic DNA. Translation: AAA24161.1.
X55791 Genomic DNA. Translation: CAA39315.1.
U00007 Genomic DNA. Translation: AAA60526.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75175.1.
AP009048 Genomic DNA. Translation: BAE76592.1.
PIRiS14427. SYECMT.
RefSeqiNP_416617.1. NC_000913.3.
WP_001350533.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75175; AAC75175; b2114.
BAE76592; BAE76592; BAE76592.
GeneIDi946643.
KEGGiecj:JW2101.
eco:b2114.
PATRICi32119561. VBIEscCol129921_2191.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02671 Genomic DNA. Translation: AAA24161.1.
X55791 Genomic DNA. Translation: CAA39315.1.
U00007 Genomic DNA. Translation: AAA60526.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75175.1.
AP009048 Genomic DNA. Translation: BAE76592.1.
PIRiS14427. SYECMT.
RefSeqiNP_416617.1. NC_000913.3.
WP_001350533.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F4LX-ray1.85A1-551[»]
1MEANMR-A139-164[»]
1MEDNMR-A139-164[»]
1P7PX-ray1.80A2-552[»]
1PFUX-ray1.91A2-552[»]
1PFVX-ray1.70A2-552[»]
1PFWX-ray1.78A2-552[»]
1PFYX-ray1.93A2-552[»]
1PG0X-ray1.90A2-552[»]
1PG2X-ray1.75A2-552[»]
1QQTX-ray2.03A2-552[»]
3H97X-ray1.70A1-548[»]
3H99X-ray1.40A1-548[»]
3H9BX-ray1.50A1-548[»]
3H9CX-ray1.40A2-548[»]
ProteinModelPortaliP00959.
SMRiP00959.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-10194N.
IntActiP00959. 3 interactors.
STRINGi511145.b2114.

Chemistry databases

BindingDBiP00959.
ChEMBLiCHEMBL3367.

2D gel databases

SWISS-2DPAGEP00959.

Proteomic databases

EPDiP00959.
PaxDbiP00959.
PRIDEiP00959.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75175; AAC75175; b2114.
BAE76592; BAE76592; BAE76592.
GeneIDi946643.
KEGGiecj:JW2101.
eco:b2114.
PATRICi32119561. VBIEscCol129921_2191.

Organism-specific databases

EchoBASEiEB0581.
EcoGeneiEG10586. metG.

Phylogenomic databases

eggNOGiENOG4105CKH. Bacteria.
COG0073. LUCA.
COG0143. LUCA.
HOGENOMiHOG000200400.
InParanoidiP00959.
KOiK01874.
OMAiFWPAMLD.
PhylomeDBiP00959.

Enzyme and pathway databases

BioCyciEcoCyc:METG-MONOMER.
ECOL316407:JW2101-MONOMER.
MetaCyc:METG-MONOMER.
BRENDAi6.1.1.10. 2026.

Miscellaneous databases

EvolutionaryTraceiP00959.
PROiP00959.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
cd02800. tRNA_bind_EcMetRS_like. 1 hit.
Gene3Di1.10.730.10. 1 hit.
2.20.28.20. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_bsu_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR029038. MetRS_Zn.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYM_ECOLI
AccessioniPrimary (citable) accession number: P00959
Secondary accession number(s): Q2MAW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 183 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.