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Protein

Methionine--tRNA ligase, cytoplasmic

Gene

MES1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of methionine to tRNA(Met) in a two-step reaction: methionine is first activated by ATP to form Met-AMP and then transferred to the acceptor end of tRNA(Met).1 Publication

Miscellaneous

Present with 85000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).3 Publications

Kineticsi

The presence of ARC1 reduces the KM for tRNA(Met) to less than 0.1 µM and increases the catalytic efficiency more than 500-fold.
  1. KM=6.6 µM for tRNA(Met) (in the absence of ARC1)3 Publications
  2. KM=10 µM for methionine3 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei411ATPBy similarity1

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • methionine-tRNA ligase activity Source: SGD

    GO - Biological processi

    • methionyl-tRNA aminoacylation Source: SGD

    Keywordsi

    Molecular functionAminoacyl-tRNA synthetase, Ligase
    Biological processProtein biosynthesis
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30933-MONOMER
    BRENDAi6.1.1.10 984

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine--tRNA ligase, cytoplasmic (EC:6.1.1.10)
    Alternative name(s):
    Methionyl-tRNA synthetase
    Short name:
    MetRS
    Gene namesi
    Name:MES1
    Ordered Locus Names:YGR264C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome VII

    Organism-specific databases

    EuPathDBiFungiDB:YGR264C
    SGDiS000003496 MES1

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi63A → H: Abolishes interaction with ARC1. 1 Publication1
    Mutagenesisi502G → D in mes1; renders the protein unstable in vitro, elevates the KM for methionine in vivo. 1 Publication1
    Mutagenesisi584N → D or Q: Abolishes aminoacylation activity. 1 Publication1
    Mutagenesisi588R → A, K or Q: Abolishes aminoacylation activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved2 Publications
    ChainiPRO_00001392692 – 751Methionine--tRNA ligase, cytoplasmicAdd BLAST750

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2N-acetylserine2 Publications1

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP00958
    PaxDbiP00958
    PRIDEiP00958

    PTM databases

    iPTMnetiP00958

    Interactioni

    Subunit structurei

    Component of a yeast aminoacyl-tRNA synthase (aaRS) complex formed by methionyl-tRNA synthase MES1, glutamyl-tRNA synthase GUS1 and the tRNA aminoacylation cofactor ARC1 in a stoichiometric complex. Interacts (via N-ter) with ARC1 (via N-ter). Can also form a stable binary complex with ARC1 that is functional in terms of aminoacylation. ARC1 increases the affinity for cognate tRNAs due to the presence of a tRNA binding domain in the middle and C-terminal part of ARC1.5 Publications

    Binary interactionsi

    Show more details

    Protein-protein interaction databases

    BioGridi33515285 interactors.
    DIPiDIP-2211N
    IntActiP00958 30 interactors.
    MINTiP00958
    STRINGi4932.YGR264C

    Structurei

    Secondary structure

    1751
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi3 – 5Combined sources3
    Beta strandi10 – 12Combined sources3
    Helixi15 – 31Combined sources17
    Beta strandi49 – 51Combined sources3
    Helixi61 – 68Combined sources8
    Turni73 – 76Combined sources4
    Helixi78 – 86Combined sources9
    Turni87 – 89Combined sources3
    Helixi90 – 92Combined sources3
    Beta strandi93 – 95Combined sources3
    Helixi98 – 111Combined sources14
    Helixi122 – 142Combined sources21
    Helixi148 – 157Combined sources10

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2HSNX-ray2.20A2-160[»]
    ProteinModelPortaliP00958
    SMRiP00958
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00958

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni36 – 92Interaction with ARC1Add BLAST57

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi205 – 215"HIGH" regionAdd BLAST11
    Motifi525 – 529"KMSKS" region5

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00550000075017
    HOGENOMiHOG000200402
    InParanoidiP00958
    KOiK01874
    OMAiYMRMAGH
    OrthoDBiEOG092C1KCQ

    Family and domain databases

    CDDicd00814 MetRS_core, 1 hit
    Gene3Di2.20.28.201 hit
    3.40.50.6202 hits
    HAMAPiMF_00098 Met_tRNA_synth_type1, 1 hit
    InterProiView protein in InterPro
    IPR001412 aa-tRNA-synth_I_CS
    IPR018285 Met-tRNA-synth_N
    IPR023458 Met-tRNA_ligase_1
    IPR014758 Met-tRNA_synth
    IPR015413 Methionyl/Leucyl_tRNA_Synth
    IPR033911 MetRS_core
    IPR029038 MetRS_Zn
    IPR014729 Rossmann-like_a/b/a_fold
    IPR009080 tRNAsynth_Ia_anticodon-bd
    PfamiView protein in Pfam
    PF09635 MetRS-N, 1 hit
    PF09334 tRNA-synt_1g, 1 hit
    PRINTSiPR01041 TRNASYNTHMET
    SUPFAMiSSF47323 SSF47323, 1 hit
    SSF57770 SSF57770, 1 hit
    TIGRFAMsiTIGR00398 metG, 1 hit
    PROSITEiView protein in PROSITE
    PS00178 AA_TRNA_LIGASE_I, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00958-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSFLISFDKS KKHPAHLQLA NNLKIALALE YASKNLKPEV DNDNAAMELR
    60 70 80 90 100
    NTKEPFLLFD ANAILRYVMD DFEGQTSDKY QFALASLQNL LYHKELPQQH
    110 120 130 140 150
    VEVLTNKAIE NYLVELKEPL TTTDLILFAN VYALNSSLVH SKFPELPSKV
    160 170 180 190 200
    HNAVALAKKH VPRDSSSFKN IGAVKIQADL TVKPKDSEIL PKPNERNILI
    210 220 230 240 250
    TSALPYVNNV PHLGNIIGSV LSADIFARYC KGRNYNALFI CGTDEYGTAT
    260 270 280 290 300
    ETKALEEGVT PRQLCDKYHK IHSDVYKWFQ IGFDYFGRTT TDKQTEIAQH
    310 320 330 340 350
    IFTKLNSNGY LEEQSMKQLY CPVHNSYLAD RYVEGECPKC HYDDARGDQC
    360 370 380 390 400
    DKCGALLDPF ELINPRCKLD DASPEPKYSD HIFLSLDKLE SQISEWVEKA
    410 420 430 440 450
    SEEGNWSKNS KTITQSWLKD GLKPRCITRD LVWGTPVPLE KYKDKVLYVW
    460 470 480 490 500
    FDATIGYVSI TSNYTKEWKQ WWNNPEHVSL YQFMGKDNVP FHTVVFPGSQ
    510 520 530 540 550
    LGTEENWTML HHLNTTEYLQ YENGKFSKSR GVGVFGNNAQ DSGISPSVWR
    560 570 580 590 600
    YYLASVRPES SDSHFSWDDF VARNNSELLA NLGNFVNRLI KFVNAKYNGV
    610 620 630 640 650
    VPKFDPKKVS NYDGLVKDIN EILSNYVKEM ELGHERRGLE IAMSLSARGN
    660 670 680 690 700
    QFLQENKLDN TLFSQSPEKS DAVVAVGLNI IYAVSSIITP YMPEIGEKIN
    710 720 730 740 750
    KMLNAPALKI DDRFHLAILE GHNINKAEYL FQRIDEKKID EWRAKYGGQQ

    V
    Length:751
    Mass (Da):85,678
    Last modified:January 23, 2007 - v4
    Checksum:i11679C1AB8BB5E39
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti122T → A in CAA24627 (PubMed:6341994).Curated1
    Sequence conflicti122T → A (PubMed:3905796).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    V01316 Genomic DNA Translation: CAA24627.1
    Y07777 Genomic DNA Translation: CAA69086.1
    Z73049 Genomic DNA Translation: CAA97293.1
    BK006941 Genomic DNA Translation: DAA08354.1
    PIRiS64597 SYBYMT
    RefSeqiNP_011780.3, NM_001181393.3

    Genome annotation databases

    EnsemblFungiiYGR264C; YGR264C; YGR264C
    GeneIDi853181
    KEGGisce:YGR264C

    Similar proteinsi

    Entry informationi

    Entry nameiSYMC_YEAST
    AccessioniPrimary (citable) accession number: P00958
    Secondary accession number(s): D6VV43
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: March 28, 2018
    This is version 181 of the entry and version 4 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome