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P00958

- SYMC_YEAST

UniProt

P00958 - SYMC_YEAST

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Protein

Methionine--tRNA ligase, cytoplasmic

Gene
MES1, YGR264C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei411 – 4111ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. methionine-tRNA ligase activity Source: SGD
  3. protein binding Source: IntAct

GO - Biological processi

  1. methionyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30933-MONOMER.
BRENDAi6.1.1.10. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase, cytoplasmic (EC:6.1.1.10)
Alternative name(s):
Methionyl-tRNA synthetase
Short name:
MetRS
Gene namesi
Name:MES1
Ordered Locus Names:YGR264C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

SGDiS000003496. MES1.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. methionyl glutamyl tRNA synthetase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi584 – 5841N → D or Q: Abolishes aminoacylation activity. 1 Publication
Mutagenesisi588 – 5881R → A, K or Q: Abolishes aminoacylation activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 751750Methionine--tRNA ligase, cytoplasmicUniRule annotationPRO_0000139269Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP00958.
PaxDbiP00958.
PeptideAtlasiP00958.

Expressioni

Gene expression databases

GenevestigatoriP00958.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
ARC1P466727EBI-18762,EBI-7224

Protein-protein interaction databases

BioGridi33515. 37 interactions.
DIPiDIP-2211N.
IntActiP00958. 10 interactions.
MINTiMINT-648422.
STRINGi4932.YGR264C.

Structurei

Secondary structure

1
751
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53
Beta strandi10 – 123
Helixi15 – 3117
Beta strandi49 – 513
Helixi61 – 688
Turni73 – 764
Helixi78 – 869
Turni87 – 893
Helixi90 – 923
Beta strandi93 – 953
Helixi98 – 11114
Helixi122 – 14221
Helixi148 – 15710

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HSNX-ray2.20A2-160[»]
ProteinModelPortaliP00958.
SMRiP00958. Positions 1-160, 199-739.

Miscellaneous databases

EvolutionaryTraceiP00958.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi205 – 21511"HIGH" regionUniRule annotationAdd
BLAST
Motifi408 – 4125"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0143.
GeneTreeiENSGT00550000075017.
HOGENOMiHOG000200402.
KOiK01874.
OMAiWVEEASE.
OrthoDBiEOG7CG77Z.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.20.28.20. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR018285. Met-tRNA-synth_N.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR029038. MetRS_Zn.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF09635. MetRS-N. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00958-1 [UniParc]FASTAAdd to Basket

« Hide

MSFLISFDKS KKHPAHLQLA NNLKIALALE YASKNLKPEV DNDNAAMELR    50
NTKEPFLLFD ANAILRYVMD DFEGQTSDKY QFALASLQNL LYHKELPQQH 100
VEVLTNKAIE NYLVELKEPL TTTDLILFAN VYALNSSLVH SKFPELPSKV 150
HNAVALAKKH VPRDSSSFKN IGAVKIQADL TVKPKDSEIL PKPNERNILI 200
TSALPYVNNV PHLGNIIGSV LSADIFARYC KGRNYNALFI CGTDEYGTAT 250
ETKALEEGVT PRQLCDKYHK IHSDVYKWFQ IGFDYFGRTT TDKQTEIAQH 300
IFTKLNSNGY LEEQSMKQLY CPVHNSYLAD RYVEGECPKC HYDDARGDQC 350
DKCGALLDPF ELINPRCKLD DASPEPKYSD HIFLSLDKLE SQISEWVEKA 400
SEEGNWSKNS KTITQSWLKD GLKPRCITRD LVWGTPVPLE KYKDKVLYVW 450
FDATIGYVSI TSNYTKEWKQ WWNNPEHVSL YQFMGKDNVP FHTVVFPGSQ 500
LGTEENWTML HHLNTTEYLQ YENGKFSKSR GVGVFGNNAQ DSGISPSVWR 550
YYLASVRPES SDSHFSWDDF VARNNSELLA NLGNFVNRLI KFVNAKYNGV 600
VPKFDPKKVS NYDGLVKDIN EILSNYVKEM ELGHERRGLE IAMSLSARGN 650
QFLQENKLDN TLFSQSPEKS DAVVAVGLNI IYAVSSIITP YMPEIGEKIN 700
KMLNAPALKI DDRFHLAILE GHNINKAEYL FQRIDEKKID EWRAKYGGQQ 750
V 751
Length:751
Mass (Da):85,678
Last modified:January 23, 2007 - v4
Checksum:i11679C1AB8BB5E39
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti122 – 1221T → A1 Publication
Sequence conflicti122 – 1221T → A1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01316 Genomic DNA. Translation: CAA24627.1.
Y07777 Genomic DNA. Translation: CAA69086.1.
Z73049 Genomic DNA. Translation: CAA97293.1.
BK006941 Genomic DNA. Translation: DAA08354.1.
PIRiS64597. SYBYMT.
RefSeqiNP_011780.3. NM_001181393.3.

Genome annotation databases

EnsemblFungiiYGR264C; YGR264C; YGR264C.
GeneIDi853181.
KEGGisce:YGR264C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01316 Genomic DNA. Translation: CAA24627.1 .
Y07777 Genomic DNA. Translation: CAA69086.1 .
Z73049 Genomic DNA. Translation: CAA97293.1 .
BK006941 Genomic DNA. Translation: DAA08354.1 .
PIRi S64597. SYBYMT.
RefSeqi NP_011780.3. NM_001181393.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2HSN X-ray 2.20 A 2-160 [» ]
ProteinModelPortali P00958.
SMRi P00958. Positions 1-160, 199-739.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33515. 37 interactions.
DIPi DIP-2211N.
IntActi P00958. 10 interactions.
MINTi MINT-648422.
STRINGi 4932.YGR264C.

Proteomic databases

MaxQBi P00958.
PaxDbi P00958.
PeptideAtlasi P00958.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR264C ; YGR264C ; YGR264C .
GeneIDi 853181.
KEGGi sce:YGR264C.

Organism-specific databases

SGDi S000003496. MES1.

Phylogenomic databases

eggNOGi COG0143.
GeneTreei ENSGT00550000075017.
HOGENOMi HOG000200402.
KOi K01874.
OMAi WVEEASE.
OrthoDBi EOG7CG77Z.

Enzyme and pathway databases

BioCyci YEAST:G3O-30933-MONOMER.
BRENDAi 6.1.1.10. 984.

Miscellaneous databases

EvolutionaryTracei P00958.
NextBioi 973317.
PROi P00958.

Gene expression databases

Genevestigatori P00958.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
2.20.28.20. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00098. Met_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR018285. Met-tRNA-synth_N.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR029038. MetRS_Zn.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view ]
Pfami PF09635. MetRS-N. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view ]
PRINTSi PR01041. TRNASYNTHMET.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsi TIGR00398. metG. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of the Saccharomyces cerevisiae gene for methionyl-tRNA synthetase."
    Walter P., Gangloff J., Bonnet J., Boulanger Y., Ebel J.-P., Fasiolo F.
    Proc. Natl. Acad. Sci. U.S.A. 80:2437-2441(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Fasiolo F.
    Submitted (SEP-1983) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Cytoplasmic methionyl-tRNA synthetase from Bakers' yeast. A monomer with a post-translationally modified N-terminus."
    Fasiolo F., Gibson B.W., Walter P., Chatton B., Biemann K., Boulanger Y.
    J. Biol. Chem. 260:15571-15576(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2.
  4. "Analysis of an 11.6 kb region from the right arm of chromosome VII of Saccharomyces cerevisiae between the RAD2 and the MES1 genes reveals the presence of three new genes."
    Clemente M.L., Sartori G., Cardazzo B., Carignani G.
    Yeast 13:287-290(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. "Strategy for the mass spectrometric verification and correction of the primary structures of proteins deduced from their DNA sequences."
    Gibson B.W., Biemann K.
    Proc. Natl. Acad. Sci. U.S.A. 81:1956-1960(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 10-90.
  8. "Identification of potential amino acid residues supporting anticodon recognition in yeast methionyl-tRNA synthetase."
    Despons L., Walter P., Senger B., Ebel J.-P., Fasiolo F.
    FEBS Lett. 289:217-220(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ASN-584 AND ARG-588.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYMC_YEAST
AccessioniPrimary (citable) accession number: P00958
Secondary accession number(s): D6VV43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 148 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 85000 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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