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Reviewed, UniProtKB/Swiss-Prot P00958 (SYMC_YEAST)

Last modified June 16, 2009. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Methionyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS
Gene names
Name: MES1
Ordered Locus Names: YGR264C
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length751 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Miscellaneous

Present with 85000 molecules/cell in log phase SD medium. Ref.8

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PRP40P332031EBI-18762,EBI-701

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 751750Methionyl-tRNA synthetase, cytoplasmic
PRO_0000139269

Regions

Motif205 – 21511"HIGH" region
Motif408 – 4125"KMSKS" region

Sites

Binding site4111ATP By similarity

Amino acid modifications

Modified residue21N-acetylserine Ref.3
Modified residue3731Phosphoserine Ref.9

Experimental info

Mutagenesis5841N → D or Q: Abolishes aminoacylation activity. Ref.7
Mutagenesis5881R → A, K or Q: Abolishes aminoacylation activity. Ref.7
Sequence conflict1221T → A Ref.1
Sequence conflict1221T → A Ref.3

Secondary structure

.......................... 751
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00958-1 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 11679C1AB8BB5E39

FASTA75185,678
        10         20         30         40         50         60 
MSFLISFDKS KKHPAHLQLA NNLKIALALE YASKNLKPEV DNDNAAMELR NTKEPFLLFD 

        70         80         90        100        110        120 
ANAILRYVMD DFEGQTSDKY QFALASLQNL LYHKELPQQH VEVLTNKAIE NYLVELKEPL 

       130        140        150        160        170        180 
TTTDLILFAN VYALNSSLVH SKFPELPSKV HNAVALAKKH VPRDSSSFKN IGAVKIQADL 

       190        200        210        220        230        240 
TVKPKDSEIL PKPNERNILI TSALPYVNNV PHLGNIIGSV LSADIFARYC KGRNYNALFI 

       250        260        270        280        290        300 
CGTDEYGTAT ETKALEEGVT PRQLCDKYHK IHSDVYKWFQ IGFDYFGRTT TDKQTEIAQH 

       310        320        330        340        350        360 
IFTKLNSNGY LEEQSMKQLY CPVHNSYLAD RYVEGECPKC HYDDARGDQC DKCGALLDPF 

       370        380        390        400        410        420 
ELINPRCKLD DASPEPKYSD HIFLSLDKLE SQISEWVEKA SEEGNWSKNS KTITQSWLKD 

       430        440        450        460        470        480 
GLKPRCITRD LVWGTPVPLE KYKDKVLYVW FDATIGYVSI TSNYTKEWKQ WWNNPEHVSL 

       490        500        510        520        530        540 
YQFMGKDNVP FHTVVFPGSQ LGTEENWTML HHLNTTEYLQ YENGKFSKSR GVGVFGNNAQ 

       550        560        570        580        590        600 
DSGISPSVWR YYLASVRPES SDSHFSWDDF VARNNSELLA NLGNFVNRLI KFVNAKYNGV 

       610        620        630        640        650        660 
VPKFDPKKVS NYDGLVKDIN EILSNYVKEM ELGHERRGLE IAMSLSARGN QFLQENKLDN 

       670        680        690        700        710        720 
TLFSQSPEKS DAVVAVGLNI IYAVSSIITP YMPEIGEKIN KMLNAPALKI DDRFHLAILE 

       730        740        750 
GHNINKAEYL FQRIDEKKID EWRAKYGGQQ V 

« Hide

References

« Hide 'large scale' references
[1]"Primary structure of the Saccharomyces cerevisiae gene for methionyl-tRNA synthetase."
Walter P., Gangloff J., Bonnet J., Boulanger Y., Ebel J.-P., Fasiolo F.
Proc. Natl. Acad. Sci. U.S.A. 80:2437-2441(1983) [PubMed: 6341994] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Fasiolo F.
Submitted (SEP-1983) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"Cytoplasmic methionyl-tRNA synthetase from Bakers' yeast. A monomer with a post-translationally modified N-terminus."
Fasiolo F., Gibson B.W., Walter P., Chatton B., Biemann K., Boulanger Y.
J. Biol. Chem. 260:15571-15576(1985) [PubMed: 3905796] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2.
[4]"Analysis of an 11.6 kb region from the right arm of chromosome VII of Saccharomyces cerevisiae between the RAD2 and the MES1 genes reveals the presence of three new genes."
Clemente M.L., Sartori G., Cardazzo B., Carignani G.
Yeast 13:287-290(1997) [PubMed: 9090059] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[5]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed: 9169869] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[6]"Strategy for the mass spectrometric verification and correction of the primary structures of proteins deduced from their DNA sequences."
Gibson B.W., Biemann K.
Proc. Natl. Acad. Sci. U.S.A. 81:1956-1960(1984) [PubMed: 6371805] [Abstract]
Cited for: PROTEIN SEQUENCE OF 10-90.
[7]"Identification of potential amino acid residues supporting anticodon recognition in yeast methionyl-tRNA synthetase."
Despons L., Walter P., Senger B., Ebel J.-P., Fasiolo F.
FEBS Lett. 289:217-220(1991) [PubMed: 1915850] [Abstract]
Cited for: MUTAGENESIS OF ASN-584 AND ARG-588.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-373, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

V01316 Genomic DNA. Translation: CAA24627.1.
Y07777 Genomic DNA. Translation: CAA69086.1.
Z73049 Genomic DNA. Translation: CAA97293.1.
PIRSYBYMT. S64597.
RefSeqNP_011780.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2HSNX-ray2.20A2-159[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:2211N.
IntActP00958. 12 interactions.

Proteomic databases

PeptideAtlasP00958.

Genome annotation databases

EnsemblYGR264C. Saccharomyces cerevisiae. [Contig view]
GeneID853181.
GenomeReviewsGene locus YGR264C in contig Y13135_GR.
KEGGsce:YGR264C.
NMPDRfig|4932.3.peg.2909.

Organism-specific databases

CYGDYGR264c.
SGDS000003496. MES1.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP00958.
OMAP00958. YINGIKH.

Enzyme and pathway databases

BRENDA6.1.1.10. 250.

Gene expression databases

ArrayExpressP00958.
GermOnlineYGR264C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR002304. Met-tRNA-synth_Ia.
IPR018285. Met-tRNA-synth_N.
IPR014729. Rossmann-like_a/b/a_fold.
IPR014758. tRNA-synt_met_N.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PfamPF09635. MetRS-N. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
TIGRFAMsTIGR00398. metG. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio973317.

Entry information

Entry nameSYMC_YEAST
AccessionPrimary (citable) accession number: P00958
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 98 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents