UniProtKB - P00954 (SYW_ECOLI)
(max 400 entries)x
Your basket is currently empty. i
Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)
Protein
Tryptophan--tRNA ligase
Gene
trpS
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the attachment of tryptophan to tRNA(Trp). Amino acylates tRNA(Trp) with both L- and D-tryptophan, although D-tryptophan is a poor substrate (PubMed:10918062).UniRule annotation1 Publication
Catalytic activityi
ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 135 | L-tryptophanUniRule annotation | 1 | |
Binding sitei | 186 | ATP; via amide nitrogen and carbonyl oxygenUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 11 – 13 | ATPUniRule annotation | 3 | |
Nucleotide bindingi | 19 – 20 | ATPUniRule annotation | 2 | |
Nucleotide bindingi | 147 – 149 | ATPUniRule annotation | 3 | |
Nucleotide bindingi | 195 – 199 | ATPUniRule annotation | 5 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- tryptophan-tRNA ligase activity Source: EcoCyc
GO - Biological processi
- tryptophanyl-tRNA aminoacylation Source: EcoCyc
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:TRPS-MONOMER. MetaCyc:TRPS-MONOMER. |
SABIO-RKi | P00954. |
Names & Taxonomyi
Protein namesi | Recommended name: Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation1 Publication)Alternative name(s): Tryptophanyl-tRNA synthetaseUniRule annotation Short name: TrpRSUniRule annotation |
Gene namesi | Name:trpSUniRule annotation Ordered Locus Names:b3384, JW3347 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Organism-specific databases
EcoGenei | EG11030. trpS. |
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000136628 | 1 – 334 | Tryptophan--tRNA ligaseAdd BLAST | 334 |
Proteomic databases
EPDi | P00954. |
PaxDbi | P00954. |
PRIDEi | P00954. |
Interactioni
Subunit structurei
Homodimer.UniRule annotation
Protein-protein interaction databases
BioGridi | 4259293. 179 interactors. |
DIPi | DIP-11042N. |
IntActi | P00954. 12 interactors. |
STRINGi | 316385.ECDH10B_3559. |
Structurei
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5V0I | X-ray | 1.90 | A/B | 1-334 | [»] | |
ProteinModelPortali | P00954. | |||||
SMRi | P00954. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 12 – 20 | "HIGH" regionUniRule annotation | 9 | |
Motifi | 195 – 199 | "KMSKS" regionUniRule annotation | 5 |
Sequence similaritiesi
Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4105C31. Bacteria. COG0180. LUCA. |
HOGENOMi | HOG000059940. |
InParanoidi | P00954. |
KOi | K01867. |
OMAi | QRMTQFK. |
PhylomeDBi | P00954. |
Family and domain databases
CDDi | cd00806. TrpRS_core. 1 hit. |
Gene3Di | 3.40.50.620. 2 hits. |
HAMAPi | MF_00140_B. Trp_tRNA_synth_B. 1 hit. |
InterProi | View protein in InterPro IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-ligase. IPR024109. Trp-tRNA-ligase_bac-type. |
Pfami | View protein in Pfam PF00579. tRNA-synt_1b. 1 hit. |
PRINTSi | PR01039. TRNASYNTHTRP. |
TIGRFAMsi | TIGR00233. trpS. 1 hit. |
PROSITEi | View protein in PROSITE PS00178. AA_TRNA_LIGASE_I. 1 hit. |
i Sequence
Sequence statusi: Complete.
P00954-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKPIVFSGA QPSGELTIGN YMGALRQWVN MQDDYHCIYC IVDQHAITVR
60 70 80 90 100
QDAQKLRKAT LDTLALYLAC GIDPEKSTIF VQSHVPEHAQ LGWALNCYTY
110 120 130 140 150
FGELSRMTQF KDKSARYAEN INAGLFDYPV LMAADILLYQ TNLVPVGEDQ
160 170 180 190 200
KQHLELSRDI AQRFNALYGE IFKVPEPFIP KSGARVMSLL EPTKKMSKSD
210 220 230 240 250
DNRNNVIGLL EDPKSVVKKI KRAVTDSDEP PVVRYDVQNK AGVSNLLDIL
260 270 280 290 300
SAVTGQSIPE LEKQFEGKMY GHLKGEVADA VSGMLTELQE RYHRFRNDEA
310 320 330
FLQQVMKDGA EKASAHASRT LKAVYEAIGF VAKP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 30 | N → K in CAA23670 (PubMed:7042706).Curated | 1 | |
Sequence conflicti | 30 | N → K in CAA79669 (PubMed:7603433).Curated | 1 | |
Sequence conflicti | 326 | E → Q in CAA23670 (PubMed:7042706).Curated | 1 | |
Sequence conflicti | 326 | E → Q in CAA79669 (PubMed:7603433).Curated | 1 | |
Sequence conflicti | 334 | P → R in CAA23670 (PubMed:7042706).Curated | 1 | |
Sequence conflicti | 334 | P → R in CAA79669 (PubMed:7603433).Curated | 1 | |
Sequence conflicti | 334 | P → R in AAA92300 (PubMed:8555191).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 60 | T → R in TRPS567C; reduced activity, thermolabile. | 1 | |
Natural varianti | 91 | L → F in TRPS10330; reduced activity. | 1 | |
Natural varianti | 112 | D → E in TRPS9969; no effect in activity. | 1 | |
Natural varianti | 129 | P → S in TRPS9969; reduced activity. | 1 | |
Natural varianti | 133 | A → E in TRPS42C; reduced activity. | 1 | |
Natural varianti | 196 | M → I in TRPS271C; activity largely reduced. | 1 | |
Natural varianti | 329 | G → S in TRPS4040; reduced activity. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00371 Genomic DNA. Translation: CAA23670.1. Z19601 Genomic DNA. Translation: CAA79669.1. U38647 Genomic DNA. Translation: AAA92300.1. U18997 Genomic DNA. Translation: AAA58181.1. U00096 Genomic DNA. Translation: AAC76409.1. AP009048 Genomic DNA. Translation: BAE77907.1. |
PIRi | C65133. YWEC. |
RefSeqi | NP_417843.1. NC_000913.3. WP_000165552.1. NZ_LN832404.1. |
Genome annotation databases
EnsemblBacteriai | AAC76409; AAC76409; b3384. BAE77907; BAE77907; BAE77907. |
GeneIDi | 947894. |
KEGGi | ecj:JW3347. eco:b3384. |
PATRICi | fig|1411691.4.peg.3346. |
Similar proteinsi
Entry informationi
Entry namei | SYW_ECOLI | |
Accessioni | P00954Primary (citable) accession number: P00954 Secondary accession number(s): Q2M749 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | August 29, 2003 | |
Last modified: | March 28, 2018 | |
This is version 152 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |