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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei198ATPBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • tryptophan-tRNA ligase activity Source: EcoCyc

GO - Biological processi

  • tryptophanyl-tRNA aminoacylation Source: EcoCyc

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:TRPS-MONOMER.
MetaCyc:TRPS-MONOMER.
SABIO-RKiP00954.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase (EC:6.1.1.2)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Gene namesi
Name:trpS
Ordered Locus Names:b3384, JW3347
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11030. trpS.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001366281 – 334Tryptophan--tRNA ligaseAdd BLAST334

Proteomic databases

PaxDbiP00954.
PRIDEiP00954.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4259293. 171 interactors.
DIPiDIP-11042N.
IntActiP00954. 12 interactors.
MINTiMINT-1244806.
STRINGi316385.ECDH10B_3559.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5V0IX-ray1.90A/B1-334[»]
ProteinModelPortaliP00954.
SMRiP00954.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 20"HIGH" region9
Motifi195 – 199"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C31. Bacteria.
COG0180. LUCA.
HOGENOMiHOG000059940.
InParanoidiP00954.
KOiK01867.
PhylomeDBiP00954.

Family and domain databases

CDDicd00806. TrpRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
PfamiView protein in Pfam
PF00579. tRNA-synt_1b. 1 hit.
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

P00954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKPIVFSGA QPSGELTIGN YMGALRQWVN MQDDYHCIYC IVDQHAITVR
60 70 80 90 100
QDAQKLRKAT LDTLALYLAC GIDPEKSTIF VQSHVPEHAQ LGWALNCYTY
110 120 130 140 150
FGELSRMTQF KDKSARYAEN INAGLFDYPV LMAADILLYQ TNLVPVGEDQ
160 170 180 190 200
KQHLELSRDI AQRFNALYGE IFKVPEPFIP KSGARVMSLL EPTKKMSKSD
210 220 230 240 250
DNRNNVIGLL EDPKSVVKKI KRAVTDSDEP PVVRYDVQNK AGVSNLLDIL
260 270 280 290 300
SAVTGQSIPE LEKQFEGKMY GHLKGEVADA VSGMLTELQE RYHRFRNDEA
310 320 330
FLQQVMKDGA EKASAHASRT LKAVYEAIGF VAKP
Length:334
Mass (Da):37,438
Last modified:August 29, 2003 - v3
Checksum:iCBAC73ABB8406026
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30N → K (PubMed:7042706).Curated1
Sequence conflicti30N → K (PubMed:7603433).Curated1
Sequence conflicti326E → Q (PubMed:7042706).Curated1
Sequence conflicti326E → Q (PubMed:7603433).Curated1
Sequence conflicti334P → R (PubMed:7042706).Curated1
Sequence conflicti334P → R (PubMed:7603433).Curated1
Sequence conflicti334P → R (PubMed:8555191).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti60T → R in TRPS567C; reduced activity, thermolabile. 1
Natural varianti91L → F in TRPS10330; reduced activity. 1
Natural varianti112D → E in TRPS9969; no effect in activity. 1
Natural varianti129P → S in TRPS9969; reduced activity. 1
Natural varianti133A → E in TRPS42C; reduced activity. 1
Natural varianti196M → I in TRPS271C; activity largely reduced. 1
Natural varianti329G → S in TRPS4040; reduced activity. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00371 Genomic DNA. Translation: CAA23670.1.
Z19601 Genomic DNA. Translation: CAA79669.1.
U38647 Genomic DNA. Translation: AAA92300.1.
U18997 Genomic DNA. Translation: AAA58181.1.
U00096 Genomic DNA. Translation: AAC76409.1.
AP009048 Genomic DNA. Translation: BAE77907.1.
PIRiC65133. YWEC.
RefSeqiNP_417843.1. NC_000913.3.
WP_000165552.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76409; AAC76409; b3384.
BAE77907; BAE77907; BAE77907.
GeneIDi947894.
KEGGiecj:JW3347.
eco:b3384.
PATRICifig|1411691.4.peg.3346.

Similar proteinsi

Entry informationi

Entry nameiSYW_ECOLI
AccessioniPrimary (citable) accession number: P00954
Secondary accession number(s): Q2M749
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 29, 2003
Last modified: August 30, 2017
This is version 147 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families