trpS

Functionⁱ

Catalyzes the attachment of tryptophan to tRNA(Trp). Amino acylates tRNA(Trp) with both L- and D-tryptophan, although D-tryptophan is a poor substrate (PubMed:10918062).UniRule annotation1 Publication

Catalytic activityⁱ

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	135	L-tryptophanUniRule annotation		1
Binding siteⁱ	186	ATP; via amide nitrogen and carbonyl oxygenUniRule annotation		1

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	11 – 13	ATPUniRule annotation	3
Nucleotide bindingⁱ	19 – 20	ATPUniRule annotation	2
Nucleotide bindingⁱ	147 – 149	ATPUniRule annotation	3
Nucleotide bindingⁱ	195 – 199	ATPUniRule annotation	5

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
tryptophan-tRNA ligase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 4371652

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

tryptophanyl-tRNA aminoacylation
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 4869215

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Aminoacyl-tRNA synthetase, Ligase
Biological process	Protein biosynthesis
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:TRPS-MONOMER. MetaCyc:TRPS-MONOMER.
SABIO-RKⁱ	P00954.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation1 Publication) Alternative name(s): Tryptophanyl-tRNA synthetaseUniRule annotation Short name: TrpRSUniRule annotation
Gene namesⁱ	Name:trpSUniRule annotation Ordered Locus Names:b3384, JW3347
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11030. trpS.

EcoGeneⁱ

EG11030. trpS.

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_00140

GO - Cellular componentⁱ

cytosol
Source: EcoCyc
Inferred from direct assayⁱ
- 15911532
- 18304323

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000136628	1 – 334	Tryptophan--tRNA ligaseAdd BLAST		334

Proteomic databases

EPDⁱ	P00954.
PaxDbⁱ	P00954.
PRIDEⁱ	P00954.

Interactionⁱ

Subunit structureⁱ

Homodimer.UniRule annotation

Protein-protein interaction databases

BioGridⁱ	4259293. 179 interactors.
Database of interacting proteins More...DIPⁱ	DIP-11042N.
IntActⁱ	P00954. 12 interactors.
STRINGⁱ	316385.ECDH10B_3559.

Structureⁱ

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	5V0I	X-ray	1.90	A/B	1-334	[»]
ProteinModelPortalⁱ	P00954.
SMRⁱ	P00954.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	12 – 20	"HIGH" regionUniRule annotation		9
Motifⁱ	195 – 199	"KMSKS" regionUniRule annotation		5

Sequence similaritiesⁱ

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105C31. Bacteria. COG0180. LUCA.
HOGENOMⁱ	HOG000059940.
InParanoidⁱ	P00954.
KEGG Orthology (KO) More...KOⁱ	K01867.
OMAⁱ	QRMTQFK.
PhylomeDBⁱ	P00954.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00806. TrpRS_core. 1 hit.
Gene3Dⁱ	3.40.50.620. 2 hits.
HAMAPⁱ	MF_00140_B. Trp_tRNA_synth_B. 1 hit.
InterProⁱ	View protein in InterPro IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-ligase. IPR024109. Trp-tRNA-ligase_bac-type.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00579. tRNA-synt_1b. 1 hit.
PRINTSⁱ	PR01039. TRNASYNTHTRP.
TIGRFAMsⁱ	TIGR00233. trpS. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

P00954-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTKPIVFSGA QPSGELTIGN YMGALRQWVN MQDDYHCIYC IVDQHAITVR 
        60         70         80         90        100
QDAQKLRKAT LDTLALYLAC GIDPEKSTIF VQSHVPEHAQ LGWALNCYTY 
       110        120        130        140        150
FGELSRMTQF KDKSARYAEN INAGLFDYPV LMAADILLYQ TNLVPVGEDQ 
       160        170        180        190        200
KQHLELSRDI AQRFNALYGE IFKVPEPFIP KSGARVMSLL EPTKKMSKSD 
       210        220        230        240        250
DNRNNVIGLL EDPKSVVKKI KRAVTDSDEP PVVRYDVQNK AGVSNLLDIL 
       260        270        280        290        300
SAVTGQSIPE LEKQFEGKMY GHLKGEVADA VSGMLTELQE RYHRFRNDEA 
       310        320        330 
FLQQVMKDGA EKASAHASRT LKAVYEAIGF VAKP

Length:334

Mass (Da):37,438

Last modified:August 29, 2003 - v3

Checksum:ⁱCBAC73ABB8406026

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	30	N → K in CAA23670 (PubMed:7042706).Curated	1
Sequence conflictⁱ	30	N → K in CAA79669 (PubMed:7603433).Curated	1
Sequence conflictⁱ	326	E → Q in CAA23670 (PubMed:7042706).Curated	1
Sequence conflictⁱ	326	E → Q in CAA79669 (PubMed:7603433).Curated	1
Sequence conflictⁱ	334	P → R in CAA23670 (PubMed:7042706).Curated	1
Sequence conflictⁱ	334	P → R in CAA79669 (PubMed:7603433).Curated	1
Sequence conflictⁱ	334	P → R in AAA92300 (PubMed:8555191).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱ	60	T → R in TRPS567C; reduced activity, thermolabile.	1
Natural variantⁱ	91	L → F in TRPS10330; reduced activity.	1
Natural variantⁱ	112	D → E in TRPS9969; no effect in activity.	1
Natural variantⁱ	129	P → S in TRPS9969; reduced activity.	1
Natural variantⁱ	133	A → E in TRPS42C; reduced activity.	1
Natural variantⁱ	196	M → I in TRPS271C; activity largely reduced.	1
Natural variantⁱ	329	G → S in TRPS4040; reduced activity.	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	V00371 Genomic DNA. Translation: CAA23670.1. Z19601 Genomic DNA. Translation: CAA79669.1. U38647 Genomic DNA. Translation: AAA92300.1. U18997 Genomic DNA. Translation: AAA58181.1. U00096 Genomic DNA. Translation: AAC76409.1. AP009048 Genomic DNA. Translation: BAE77907.1.
PIRⁱ	C65133. YWEC.
NCBI Reference Sequences More...RefSeqⁱ	NP_417843.1. NC_000913.3. WP_000165552.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaⁱ	AAC76409; AAC76409; b3384. BAE77907; BAE77907; BAE77907.
GeneIDⁱ	947894.
KEGGⁱ	ecj:JW3347. eco:b3384.
PATRICⁱ	fig\|1411691.4.peg.3346.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P00954	W1B9B5 A0A236RCD9 E2QFN4 F4SQB4 A0A025CP33 A0A023Z3N5 A0A073H280 D7Y643 M9FNI4 A0A140N7C1 +59	Klebsiella pneumoniae IS22 Shigella boydii Escherichia coli Escherichia coli H736 Escherichia coli O145:NM str. 2010C-3526 Escherichia coli O145:H28 str. RM12581 Escherichia coli 5-366-08_S1_C3 Escherichia coli MS 115-1 Escherichia coli MP021561.2 Escherichia coli (strain B / BL21-DE3) And more	334	UniRef100_P00954	Cluster: Tryptophan--tRNA ligase	70

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P00954	W1B9B5 A0A236RCD9 E2QFN4 F4SQB4 A0A025CP33 A0A023Z3N5 A0A073H280 D7Y643 M9FNI4 A0A140N7C1 +802	Klebsiella pneumoniae IS22 Shigella boydii Escherichia coli Escherichia coli H736 Escherichia coli O145:NM str. 2010C-3526 Escherichia coli O145:H28 str. RM12581 Escherichia coli 5-366-08_S1_C3 Escherichia coli MS 115-1 Escherichia coli MP021561.2 Escherichia coli (strain B / BL21-DE3) And more	334	UniRef90_P00954	Cluster: Tryptophan--tRNA ligase	813

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P00954	W1B9B5 A0A236RCD9 E2QFN4 F4SQB4 A0A025CP33 A0A023Z3N5 A0A073H280 D7Y643 M9FNI4 A0A140N7C1 +1412	Klebsiella pneumoniae IS22 Shigella boydii Escherichia coli Escherichia coli H736 Escherichia coli O145:NM str. 2010C-3526 Escherichia coli O145:H28 str. RM12581 Escherichia coli 5-366-08_S1_C3 Escherichia coli MS 115-1 Escherichia coli MP021561.2 Escherichia coli (strain B / BL21-DE3) And more	334	UniRef50_P00954	Cluster: Tryptophan--tRNA ligase	1,423

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	V00371 Genomic DNA. Translation: CAA23670.1. Z19601 Genomic DNA. Translation: CAA79669.1. U38647 Genomic DNA. Translation: AAA92300.1. U18997 Genomic DNA. Translation: AAA58181.1. U00096 Genomic DNA. Translation: AAC76409.1. AP009048 Genomic DNA. Translation: BAE77907.1.
PIRⁱ	C65133. YWEC.
NCBI Reference Sequences More...RefSeqⁱ	NP_417843.1. NC_000913.3. WP_000165552.1. NZ_LN832404.1.

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	5V0I	X-ray	1.90	A/B	1-334	[»]
ProteinModelPortalⁱ	P00954.
SMRⁱ	P00954.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4259293. 179 interactors.
Database of interacting proteins More...DIPⁱ	DIP-11042N.
IntActⁱ	P00954. 12 interactors.
STRINGⁱ	316385.ECDH10B_3559.

Proteomic databases

EPDⁱ	P00954.
PaxDbⁱ	P00954.
PRIDEⁱ	P00954.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC76409; AAC76409; b3384. BAE77907; BAE77907; BAE77907.
GeneIDⁱ	947894.
KEGGⁱ	ecj:JW3347. eco:b3384.
PATRICⁱ	fig\|1411691.4.peg.3346.

Organism-specific databases

EchoBASEⁱ	EB1023.
EcoGeneⁱ	EG11030. trpS.

Phylogenomic databases

eggNOGⁱ	ENOG4105C31. Bacteria. COG0180. LUCA.
HOGENOMⁱ	HOG000059940.
InParanoidⁱ	P00954.
KEGG Orthology (KO) More...KOⁱ	K01867.
OMAⁱ	QRMTQFK.
PhylomeDBⁱ	P00954.

Enzyme and pathway databases

BioCycⁱ	EcoCyc:TRPS-MONOMER. MetaCyc:TRPS-MONOMER.
SABIO-RKⁱ	P00954.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P00954.

PROⁱ

PR:P00954.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00806. TrpRS_core. 1 hit.
Gene3Dⁱ	3.40.50.620. 2 hits.
HAMAPⁱ	MF_00140_B. Trp_tRNA_synth_B. 1 hit.
InterProⁱ	View protein in InterPro IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-ligase. IPR024109. Trp-tRNA-ligase_bac-type.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00579. tRNA-synt_1b. 1 hit.
PRINTSⁱ	PR01039. TRNASYNTHTRP.
TIGRFAMsⁱ	TIGR00233. trpS. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00178. AA_TRNA_LIGASE_I. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SYW_ECOLI
Accessionⁱ	P00954Primary (citable) accession number: P00954 Secondary accession number(s): Q2M749
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
	Last sequence update:	August 29, 2003
	Last modified:	March 28, 2018
	This is version 152 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

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UniProtKB - P00954 (SYW_ECOLI)

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Tryptophan--tRNA ligase

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ