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P00953

- SYW_GEOSE

UniProt

P00953 - SYW_GEOSE

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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei195 – 1951ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. tryptophan-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tryptophanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase (EC:6.1.1.2)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Gene namesi
Name:trpS
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Tryptophan--tRNA ligasePRO_0000136601Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

DIPiDIP-48697N.

Structurei

Secondary structure

1
328
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86Combined sources
Helixi16 – 216Combined sources
Helixi23 – 297Combined sources
Turni30 – 323Combined sources
Beta strandi33 – 397Combined sources
Helixi41 – 444Combined sources
Helixi51 – 6717Combined sources
Turni72 – 743Combined sources
Beta strandi75 – 795Combined sources
Helixi80 – 823Combined sources
Helixi85 – 9612Combined sources
Helixi99 – 1035Combined sources
Helixi106 – 1127Combined sources
Helixi120 – 1234Combined sources
Helixi125 – 13410Combined sources
Turni135 – 1373Combined sources
Beta strandi139 – 1424Combined sources
Helixi145 – 1473Combined sources
Helixi148 – 16518Combined sources
Beta strandi173 – 1753Combined sources
Beta strandi178 – 1825Combined sources
Helixi200 – 2023Combined sources
Helixi210 – 21910Combined sources
Turni232 – 2343Combined sources
Helixi236 – 24914Combined sources
Helixi253 – 2597Combined sources
Turni260 – 2623Combined sources
Helixi265 – 29127Combined sources
Helixi295 – 32228Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D2RX-ray2.90A/B/C/D/E/F1-326[»]
1I6KX-ray1.72A1-328[»]
1I6LX-ray1.72A1-328[»]
1I6MX-ray1.72A1-328[»]
1M83X-ray2.20A1-328[»]
1MAUX-ray2.15A1-328[»]
1MAWX-ray3.00A/B/C/D/E/F1-328[»]
1MB2X-ray2.70A/B/C/D/E/F1-328[»]
2OV4X-ray2.50A1-328[»]
3FHJX-ray2.65A/B/C/D/E/F1-326[»]
3FI0X-ray2.70A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R1-326[»]
ProteinModelPortaliP00953.
SMRiP00953. Positions 1-328.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00953.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi10 – 189"HIGH" region
Motifi192 – 1965"KMSKS" region

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00953-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKTIFSGIQP SGVITIGNYI GALRQFVELQ HEYNCYFCIV DQHAITVWQD
60 70 80 90 100
PHELRQNIRR LAAKYLAVGI DPTQATLFIQ SEVPAHAQAA WMLQCIVYIG
110 120 130 140 150
ELERMTQFKE KSAGKEAVSA GLLTYPPLMA ADILLYNTDI VPVGEDQKQH
160 170 180 190 200
IELTRDLAER FNKRYGELFT IPEARIPKVG ARIMSLVDPT KKMSKSDPNP
210 220 230 240 250
KAYITLLDDA KTIEKKIKSA VTDSEGTIRY DKEAKPGISN LLNIYSTLSG
260 270 280 290 300
QSIEELERQY EGKGYGVFKA DLAQVVIETL RPIQERYHHW MESEELDRVL
310 320
DEGAEKANRV ASEMVRKMEQ AMGLGRRR
Length:328
Mass (Da):37,193
Last modified:November 1, 1988 - v1
Checksum:iB8C4C6028F0913B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14742 Genomic DNA. Translation: AAA22873.1.
PIRiA26055. YWBSF.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14742 Genomic DNA. Translation: AAA22873.1 .
PIRi A26055. YWBSF.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1D2R X-ray 2.90 A/B/C/D/E/F 1-326 [» ]
1I6K X-ray 1.72 A 1-328 [» ]
1I6L X-ray 1.72 A 1-328 [» ]
1I6M X-ray 1.72 A 1-328 [» ]
1M83 X-ray 2.20 A 1-328 [» ]
1MAU X-ray 2.15 A 1-328 [» ]
1MAW X-ray 3.00 A/B/C/D/E/F 1-328 [» ]
1MB2 X-ray 2.70 A/B/C/D/E/F 1-328 [» ]
2OV4 X-ray 2.50 A 1-328 [» ]
3FHJ X-ray 2.65 A/B/C/D/E/F 1-326 [» ]
3FI0 X-ray 2.70 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R 1-326 [» ]
ProteinModelPortali P00953.
SMRi P00953. Positions 1-328.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48697N.

Chemistry

DrugBanki DB00150. L-Tryptophan.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P00953.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
HAMAPi MF_00140_B. Trp_tRNA_synth_B.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view ]
PANTHERi PTHR10055. PTHR10055. 1 hit.
Pfami PF00579. tRNA-synt_1b. 1 hit.
[Graphical view ]
PRINTSi PR01039. TRNASYNTHTRP.
TIGRFAMsi TIGR00233. trpS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and complete nucleotide sequence of the Bacillus stearothermophilus tryptophanyl tRNA synthetase gene."
    Barstow D.A., Sharman A.F., Atkinson T., Minton N.P.
    Gene 46:37-45(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The amino acid sequence of tryptophanyl tRNA synthetase from Bacillus stearothermophilus."
    Winter G.P., Hartley B.S.
    FEBS Lett. 80:340-342(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-327.
  3. "Tryptophanyl-tRNA synthetase crystal structure reveals an unexpected homology to tyrosyl-tRNA synthetase."
    Doublie S., Bricogne G., Gilmore C., Carter C.W. Jr.
    Structure 3:17-31(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
  4. "2.9-A crystal structure of ligand-free tryptophanyl-tRNA synthetase: domain movements fragment the adenine nucleotide binding site."
    Ilyin V.A., Temple B., Hu M., Li G.-P., Yin Y., Vachette P., Carter C.W. Jr.
    Protein Sci. 9:218-231(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).

Entry informationi

Entry nameiSYW_GEOSE
AccessioniPrimary (citable) accession number: P00953
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1988
Last modified: November 26, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3