Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei195ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.2. 623.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase (EC:6.1.1.2)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Gene namesi
Name:trpS
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

DrugBankiDB00150. L-Tryptophan.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001366011 – 328Tryptophan--tRNA ligaseAdd BLAST328

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

DIPiDIP-48697N.

Structurei

Secondary structure

1328
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Beta strandi11 – 13Combined sources3
Helixi16 – 21Combined sources6
Helixi23 – 29Combined sources7
Turni30 – 32Combined sources3
Beta strandi33 – 39Combined sources7
Helixi41 – 44Combined sources4
Helixi51 – 67Combined sources17
Turni72 – 74Combined sources3
Beta strandi75 – 79Combined sources5
Helixi80 – 82Combined sources3
Helixi85 – 96Combined sources12
Helixi99 – 103Combined sources5
Helixi106 – 112Combined sources7
Helixi120 – 123Combined sources4
Helixi125 – 134Combined sources10
Turni135 – 137Combined sources3
Beta strandi139 – 142Combined sources4
Helixi145 – 147Combined sources3
Helixi148 – 165Combined sources18
Beta strandi173 – 175Combined sources3
Beta strandi178 – 182Combined sources5
Helixi200 – 202Combined sources3
Helixi210 – 219Combined sources10
Turni232 – 234Combined sources3
Helixi236 – 249Combined sources14
Helixi253 – 259Combined sources7
Turni260 – 262Combined sources3
Helixi265 – 291Combined sources27
Helixi295 – 322Combined sources28

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D2RX-ray2.90A/B/C/D/E/F1-326[»]
1I6KX-ray1.72A1-328[»]
1I6LX-ray1.72A1-328[»]
1I6MX-ray1.72A1-328[»]
1M83X-ray2.20A1-328[»]
1MAUX-ray2.15A1-328[»]
1MAWX-ray3.00A/B/C/D/E/F1-328[»]
1MB2X-ray2.70A/B/C/D/E/F1-328[»]
2OV4X-ray2.50A1-328[»]
3FHJX-ray2.65A/B/C/D/E/F1-326[»]
3FI0X-ray2.70A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R1-326[»]
5DK4X-ray1.90A2-328[»]
ProteinModelPortaliP00953.
SMRiP00953.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00953.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi10 – 18"HIGH" region9
Motifi192 – 196"KMSKS" region5

Sequence similaritiesi

Family and domain databases

CDDicd00806. TrpRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00953-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTIFSGIQP SGVITIGNYI GALRQFVELQ HEYNCYFCIV DQHAITVWQD
60 70 80 90 100
PHELRQNIRR LAAKYLAVGI DPTQATLFIQ SEVPAHAQAA WMLQCIVYIG
110 120 130 140 150
ELERMTQFKE KSAGKEAVSA GLLTYPPLMA ADILLYNTDI VPVGEDQKQH
160 170 180 190 200
IELTRDLAER FNKRYGELFT IPEARIPKVG ARIMSLVDPT KKMSKSDPNP
210 220 230 240 250
KAYITLLDDA KTIEKKIKSA VTDSEGTIRY DKEAKPGISN LLNIYSTLSG
260 270 280 290 300
QSIEELERQY EGKGYGVFKA DLAQVVIETL RPIQERYHHW MESEELDRVL
310 320
DEGAEKANRV ASEMVRKMEQ AMGLGRRR
Length:328
Mass (Da):37,193
Last modified:November 1, 1988 - v1
Checksum:iB8C4C6028F0913B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14742 Genomic DNA. Translation: AAA22873.1.
PIRiA26055. YWBSF.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14742 Genomic DNA. Translation: AAA22873.1.
PIRiA26055. YWBSF.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D2RX-ray2.90A/B/C/D/E/F1-326[»]
1I6KX-ray1.72A1-328[»]
1I6LX-ray1.72A1-328[»]
1I6MX-ray1.72A1-328[»]
1M83X-ray2.20A1-328[»]
1MAUX-ray2.15A1-328[»]
1MAWX-ray3.00A/B/C/D/E/F1-328[»]
1MB2X-ray2.70A/B/C/D/E/F1-328[»]
2OV4X-ray2.50A1-328[»]
3FHJX-ray2.65A/B/C/D/E/F1-326[»]
3FI0X-ray2.70A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R1-326[»]
5DK4X-ray1.90A2-328[»]
ProteinModelPortaliP00953.
SMRiP00953.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48697N.

Chemistry databases

DrugBankiDB00150. L-Tryptophan.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi6.1.1.2. 623.

Miscellaneous databases

EvolutionaryTraceiP00953.

Family and domain databases

CDDicd00806. TrpRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYW_GEOSE
AccessioniPrimary (citable) accession number: P00953
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1988
Last modified: November 30, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.