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P00949 (PGM1_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglucomutase-1
Short name=PGM 1
EC=5.4.2.2
Alternative name(s):
Glucose phosphomutase 1
Gene names
Name:PGM1
OrganismOryctolagus cuniculus (Rabbit) [Complete proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length562 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme participates in both the breakdown and synthesis of glucose.

Catalytic activity

Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate.

Cofactor

Binds 1 magnesium ion per subunit.

Subunit structure

Monomer.

Subcellular location

Isoform 1: Cytoplasm.

Isoform 2: Sarcoplasmic reticulum. Note: Localizes to the junctional skeletal sarcoplasmic reticulum, probably by association with phospholipids and/or other proteins.

Post-translational modification

Isoform 2 is the major calmodulin-dependent phosphoprotein in junctional skeletal sarcoplasmic reticulum vesicles.

Sequence similarities

Belongs to the phosphohexose mutase family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Glucose metabolism
   Cellular componentCytoplasm
Sarcoplasmic reticulum
   Coding sequence diversityAlternative splicing
   LigandMagnesium
Metal-binding
   Molecular functionIsomerase
   PTMAcetylation
Phosphoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processglucose metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentsarcoplasmic reticulum

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionmagnesium ion binding

Inferred from electronic annotation. Source: InterPro

phosphoglucomutase activity

Inferred from sequence or structural similarity. Source: AgBase

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P00949-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P00949-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: MVKIVTVKTKAYP → MEEGPLPLLTIRTAPYH
     25-36: RVKVFQSSTNYA → KTYYFEDKPCYL
     44-56: ISTVEPAQRQEAT → FFSIDLKDRQGSS
     65-77: FYMKEAIQLIVRI → YFNKSAIETILQM

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 562561Phosphoglucomutase-1
PRO_0000147779

Sites

Active site1171Phosphoserine intermediate Ref.4
Metal binding1171Magnesium; via phosphate group
Metal binding2881Magnesium
Metal binding2901Magnesium
Metal binding2921Magnesium

Amino acid modifications

Modified residue161N6-acetyllysine By similarity
Modified residue1151Phosphothreonine By similarity
Modified residue1171Phosphoserine By similarity
Modified residue3531Phosphotyrosine By similarity
Modified residue4191N6-acetyllysine By similarity

Natural variations

Alternative sequence1 – 1313MVKIV…TKAYP → MEEGPLPLLTIRTAPYH in isoform 2.
VSP_004690
Alternative sequence25 – 3612RVKVF…STNYA → KTYYFEDKPCYL in isoform 2.
VSP_004691
Alternative sequence44 – 5613ISTVE…RQEAT → FFSIDLKDRQGSS in isoform 2.
VSP_004692
Alternative sequence65 – 7713FYMKE…LIVRI → YFNKSAIETILQM in isoform 2.
VSP_004693

Secondary structure

1 ......................................................................................................... 562
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 6D9F42284EF09002

FASTA56261,558
        10         20         30         40         50         60 
MVKIVTVKTK AYPDQKPGTS GLRKRVKVFQ SSTNYAENFI QSIISTVEPA QRQEATLVVG 

        70         80         90        100        110        120 
GDGRFYMKEA IQLIVRIAAA NGIGRLVIGQ NGILSTPAVS CIIRKIKAIG GIILTASHNP 

       130        140        150        160        170        180 
GGPNGDFGIK FNISNGGPAP EAITDKIFQI SKTIEEYAIC PDLKVDLGVL GKQQFDLENK 

       190        200        210        220        230        240 
FKPFTVEIVD SVEAYATMLR NIFDFNALKE LLSGPNRLKI RIDAMHGVVG PYVKKILCEE 

       250        260        270        280        290        300 
LGAPANSAVN CVPLEDFGGH HPDPNLTYAA DLVETMKSGE HDFGAAFDGD GDRNMILGKH 

       310        320        330        340        350        360 
GFFVNPSDSV AVIAANIFSI PYFQQTGVRG FARSMPTSGA LDRVANATKI ALYETPTGWK 

       370        380        390        400        410        420 
FFGNLMDASK LSLCGEESFG TGSDHIREKD GLWAVLAWLS ILATRKQSVE DILKDHWHKF 

       430        440        450        460        470        480 
GRNFFTRYDY EEVEAEGATK MMKDLEALMF DRSFVGKQFS ANDKVYTVEK ADNFEYHDPV 

       490        500        510        520        530        540 
DGSVSKNQGL RLIFADGSRI IFRLSGTGSA GATIRLYIDS YEKDNAKINQ DPQVMLAPLI 

       550        560 
SIALKVSQLQ ERTGRTAPTV IT

« Hide

Isoform 2 [UniParc].

Checksum: DB76D766775F9F13
Show »

FASTA56662,193

References

[1]"The complete amino acid sequence of rabbit muscle phosphoglucomutase."
Ray W.J. Jr., Hermodson M.A., Puvathingal J.M., Mahoney W.C.
J. Biol. Chem. 258:9166-9174(1983) [PubMed: 6223925] [Abstract]
Cited for: PROTEIN SEQUENCE (ISOFORM 1).
Tissue: Muscle.
[2]"Phosphoglucomutase 1: complete human and rabbit mRNA sequences and direct mapping of this highly polymorphic marker on human chromosome 1."
Whitehouse D.B., Putt W., Lovegrove J.U., Morrison K.E., Hollyoake M., Fox M.F., Hopkinson D.A., Edwards Y.H.
Proc. Natl. Acad. Sci. U.S.A. 89:411-415(1992) [PubMed: 1530890] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Purification, characterization, and molecular cloning of a 60-kDa phosphoprotein in rabbit skeletal sarcoplasmic reticulum which is an isoform of phosphoglucomutase."
Lee Y.S., Marks A.R., Gureckas N., Lacro R., Nadal-Ginard B., Kim D.H.
J. Biol. Chem. 267:21080-21088(1992) [PubMed: 1328221] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Skeletal muscle.
[4]"A tryptic peptide containing a unique serine phosphate residue in rabbit phosphoglucomutase."
Milstein C.P., Milstein C.
Biochem. J. 109:93-99(1968) [PubMed: 5669853] [Abstract]
Cited for: ACTIVE SITE.
[5]"The crystal structure of muscle phosphoglucomutase refined at 2.7-A resolution."
Dai J.-B., Liu Y., Ray W.J. Jr., Konno M.
J. Biol. Chem. 267:6322-6337(1992) [PubMed: 1532581] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
[6]"Structure of rabbit muscle phosphoglucomutase refined at 2.4-A resolution."
Liu Y., Ray W.J. Jr., Baranidharan S.
Acta Crystallogr. D 53:392-405(1997) [PubMed: 15299905] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M97664 mRNA. Translation: AAA31454.1.
M97663 mRNA. Translation: AAA31453.1.
PIRPMRBI. A45077.
PMRB. B41801.
RefSeqNP_001075785.1. NM_001082316.1.
UniGeneOcu.1953.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1C47X-ray2.70A/B2-562[»]
1C4GX-ray2.70A/B2-562[»]
1JDYX-ray2.70A/B2-561[»]
1LXTX-ray2.70A/B2-562[»]
1VKLX-ray2.70A/B2-561[»]
3PMGX-ray2.40A/B2-562[»]
ProteinModelPortalP00949.
SMRP00949. Positions 2-562.
ModBaseSearch...

Protein-protein interaction databases

STRINGP00949.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100009155.

Organism-specific databases

CTD5236.

Phylogenomic databases

eggNOGmaNOG12728.
GeneTreeENSGT00390000011831.
HOVERGENHBG001599.

Family and domain databases

InterProIPR005844. A-D-PHexomutase_a/b/a-I.
IPR016055. A-D-PHexomutase_a/b/a-I/II/III.
IPR005845. A-D-PHexomutase_a/b/a-II.
IPR005846. A-D-PHexomutase_a/b/a-III.
IPR005843. A-D-PHexomutase_C.
IPR016066. A-D-PHexomutase_CS.
IPR005841. Alpha-D-phosphohexomutase_SF.
[Graphical view]
Gene3DG3DSA:3.40.120.10. A-D-PHexomutase_a/b/a-I/II/III. 3 hits.
PfamPF02878. PGM_PMM_I. 1 hit.
PF02879. PGM_PMM_II. 1 hit.
PF02880. PGM_PMM_III. 1 hit.
PF00408. PGM_PMM_IV. 1 hit.
[Graphical view]
PRINTSPR00509. PGMPMM.
SUPFAMSSF53738. A-D-PHexomutase_a/b/a-I/II/III. 2 hits.
PROSITEPS00710. PGM_PMM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGM1_RABIT
AccessionPrimary (citable) accession number: P00949
Secondary accession number(s): P38651
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 16, 2011
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents