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Protein

Muconolactone Delta-isomerase

Gene

catC

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-5-oxo-2,5-dihydrofuran-2-acetate = 5-oxo-4,5-dihydrofuran-2-acetate.

Pathwayi: beta-ketoadipate pathway

This protein is involved in step 3 of the subpathway that synthesizes 5-oxo-4,5-dihydro-2-furylacetate from catechol.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Muconate cycloisomerase 1 (catB)
  3. Muconolactone Delta-isomerase (catC), Muconolactone Delta-isomerase (catC), Muconolactone Delta-isomerase (catC), Muconolactone Delta-isomerase, Muconolactone Delta-isomerase (AL481_22130), Muconolactone Delta-isomerase (catC), Muconolactone Delta-isomerase (HB4184_27310), Muconolactone Delta-isomerase (PU99_13590), Muconolactone Delta-isomerase, Muconolactone Delta-isomerase (HA62_11725)
This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-oxo-4,5-dihydro-2-furylacetate from catechol, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3424.
UniPathwayiUPA00157; UER00260.

Names & Taxonomyi

Protein namesi
Recommended name:
Muconolactone Delta-isomerase (EC:5.3.3.4)
Short name:
MIase
Gene namesi
Name:catC
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9696Muconolactone Delta-isomerasePRO_0000089334Add
BLAST

Interactioni

Subunit structurei

Homodecamer.

Protein-protein interaction databases

STRINGi76869.PputGB1_2205.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MLIX-ray3.30A/B/C/D/E/F/G/H/I/J1-96[»]
ProteinModelPortaliP00948.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00948.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105NN7. Bacteria.
COG4829. LUCA.

Family and domain databases

InterProiIPR011008. Dimeric_a/b-barrel.
IPR026029. MLI_dom.
IPR003464. Muconolactone_d_Isoase.
[Graphical view]
PfamiPF02426. MIase. 1 hit.
[Graphical view]
PIRSFiPIRSF001486. CatC. 1 hit.
ProDomiPD006619. Muconolactone_d_Isoase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF54909. SSF54909. 1 hit.
TIGRFAMsiTIGR03221. muco_delta. 1 hit.

Sequencei

Sequence statusi: Complete.

P00948-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFHVKMTVK LPVDMDPAKA TQLKADEKEL AQRLQREGTW RHLWRIAGHY
60 70 80 90
ANYSVFDVSS VEACNDTLMQ LPLFPYMDIE VDGLCRHPSS IHSDDR
Length:96
Mass (Da):11,116
Last modified:July 15, 1999 - v3
Checksum:i0BD82946E0636D14
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti21 – 222TQ → AR in AAA25767 (PubMed:2449420).Curated
Sequence conflicti47 – 471A → AV in AAA25767 (PubMed:2449420).Curated
Sequence conflicti59 – 591S → P in AAA66203 (PubMed:7814330).Curated
Sequence conflicti64 – 652CN → LH in AAA66203 (PubMed:7814330).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19460 Genomic DNA. Translation: AAA25767.1.
U12557 Genomic DNA. Translation: AAA66203.1.
PIRiB28630. ISPSMP.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19460 Genomic DNA. Translation: AAA25767.1.
U12557 Genomic DNA. Translation: AAA66203.1.
PIRiB28630. ISPSMP.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MLIX-ray3.30A/B/C/D/E/F/G/H/I/J1-96[»]
ProteinModelPortaliP00948.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi76869.PputGB1_2205.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105NN7. Bacteria.
COG4829. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00157; UER00260.
BioCyciMetaCyc:MONOMER-3424.

Miscellaneous databases

EvolutionaryTraceiP00948.

Family and domain databases

InterProiIPR011008. Dimeric_a/b-barrel.
IPR026029. MLI_dom.
IPR003464. Muconolactone_d_Isoase.
[Graphical view]
PfamiPF02426. MIase. 1 hit.
[Graphical view]
PIRSFiPIRSF001486. CatC. 1 hit.
ProDomiPD006619. Muconolactone_d_Isoase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF54909. SSF54909. 1 hit.
TIGRFAMsiTIGR03221. muco_delta. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Transcriptional regulation, nucleotide sequence, and localization of the promoter of the catBC operon in Pseudomonas putida."
    Aldrich T.L., Chakrabarty A.M.
    J. Bacteriol. 170:1297-1304(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Discontinuities in the evolution of Pseudomonas putida cat genes."
    Houghton J.E., Brown T.M., Appel A.J., Hughes E.J., Ornston L.N.
    J. Bacteriol. 177:401-412(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: PRS2000.
  3. "Repetitions in the NH2-terminal amino acid sequence of beta-ketoadipate enol-lactone hydrolase from Pseudomonas putida."
    McCorkle G.M., Yeh W.-K., Fletcher P., Ornston L.N.
    J. Biol. Chem. 255:6335-6341(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-52.
  4. "Purification and partial amino acid sequence of the cyanogen bromide fragments of muconolactone isomerase from Pseudomonas putida."
    Meagher R.B.
    Biochim. Biophys. Acta 494:33-47(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
    Strain: PRS2113.
  5. "Crystal structure of muconolactone isomerase at 3.3-A resolution."
    Katti S.K., Katz B.A., Wyckoff H.W.
    J. Mol. Biol. 205:557-571(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS).

Entry informationi

Entry nameiCATC_PSEPU
AccessioniPrimary (citable) accession number: P00948
Secondary accession number(s): Q51959
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 15, 1999
Last modified: April 13, 2016
This is version 79 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.