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Protein

Steroid Delta-isomerase

Gene

ksi

Organism
Comamonas testosteroni (Pseudomonas testosteroni)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei14Proton donor1
Active sitei38Proton acceptor1 Publication1
Binding sitei99Substrate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Lipid metabolism, Steroid metabolism

Enzyme and pathway databases

BRENDAi5.3.3.1. 1590.
SABIO-RKP00947.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid Delta-isomerase (EC:5.3.3.1)
Alternative name(s):
Delta(5)-3-ketosteroid isomerase
Gene namesi
Name:ksi
OrganismiComamonas testosteroni (Pseudomonas testosteroni)
Taxonomic identifieri285 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeComamonas

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39P → A: Perturbs active site geometry and lowers activity. 1
Mutagenesisi99D → A: Lowers activity 3000-fold. 1 Publication1
Mutagenesisi116F → W: Slightly lower activity at neutral pH. Increased catalytic activity at pH 3.8. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000976441 – 125Steroid Delta-isomeraseAdd BLAST125

Expressioni

Inductioni

By steroids.

Interactioni

Subunit structurei

Homodimer.3 Publications

Protein-protein interaction databases

STRINGi688245.CtCNB1_1323.

Structurei

Secondary structure

1125
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 20Combined sources17
Helixi23 – 27Combined sources5
Beta strandi30 – 39Combined sources10
Beta strandi45 – 47Combined sources3
Helixi48 – 58Combined sources11
Beta strandi64 – 67Combined sources4
Beta strandi72 – 74Combined sources3
Beta strandi77 – 88Combined sources12
Beta strandi91 – 95Combined sources5
Beta strandi98 – 103Combined sources6
Turni105 – 107Combined sources3
Beta strandi109 – 115Combined sources7
Helixi118 – 120Combined sources3
Beta strandi121 – 123Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BUQNMR-A/B1-125[»]
1ISKNMR-A/B1-125[»]
1OCVX-ray2.00A/B/C/D1-125[»]
1OGZX-ray2.30A1-125[»]
1OHPX-ray1.53A/B/C/D1-125[»]
1OHSX-ray1.70A/B/C/D1-125[»]
1QJGX-ray2.30A/B/C/D/E/F1-125[»]
3M8CX-ray2.10A/B/C/D1-125[»]
3MHEX-ray1.72A/B1-125[»]
3MKIX-ray2.00A/B/C/D1-125[»]
3MYTX-ray1.96A/B/C/D1-125[»]
3NBRX-ray1.73A1-125[»]
3NHXX-ray1.59A1-125[»]
3NM2X-ray1.89A1-125[»]
3NUVX-ray1.76A/B1-125[»]
3NXJX-ray1.97A/B1-125[»]
3OV4X-ray1.83A/B/C/D1-125[»]
3T8UX-ray2.50A/B/C/D1-125[»]
3UNLX-ray2.52A/B/C/D1-125[»]
4L7KX-ray2.10A/B/C/D/E/F/G/H/I/J/K/O1-125[»]
5DREX-ray2.15A1-125[»]
8CHOX-ray2.30A1-125[»]
ProteinModelPortaliP00947.
SMRiP00947.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00947.

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105X1P. Bacteria.
ENOG411288K. LUCA.

Family and domain databases

Gene3Di3.10.450.50. 1 hit.
InterProiIPR002075. NTF2.
IPR032710. NTF2-like_dom.
IPR011944. Steroid_delta5-4_isomerase.
[Graphical view]
PfamiPF02136. NTF2. 1 hit.
[Graphical view]
SUPFAMiSSF54427. SSF54427. 1 hit.
TIGRFAMsiTIGR02246. TIGR02246. 1 hit.

Sequencei

Sequence statusi: Complete.

P00947-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTPEHMTAV VQRYVAALNA GDLDGIVALF ADDATVEDPV GSEPRSGTAA
60 70 80 90 100
IREFYANSLK LPLAVELTQE VRAVANEAAF AFTVSFEYQG RKTVVAPIDH
110 120
FRFNGAGKVV SMRALFGEKN IHAGA
Length:125
Mass (Da):13,398
Last modified:February 1, 1991 - v2
Checksum:i2ECD410D4B929430
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22749 Genomic DNA. Translation: AAA25872.1.
J03568 Genomic DNA. Translation: AAA25871.1.
PIRiJT0336. SIPSDT.
RefSeqiWP_003078309.1. NZ_BBJZ01000017.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22749 Genomic DNA. Translation: AAA25872.1.
J03568 Genomic DNA. Translation: AAA25871.1.
PIRiJT0336. SIPSDT.
RefSeqiWP_003078309.1. NZ_BBJZ01000017.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BUQNMR-A/B1-125[»]
1ISKNMR-A/B1-125[»]
1OCVX-ray2.00A/B/C/D1-125[»]
1OGZX-ray2.30A1-125[»]
1OHPX-ray1.53A/B/C/D1-125[»]
1OHSX-ray1.70A/B/C/D1-125[»]
1QJGX-ray2.30A/B/C/D/E/F1-125[»]
3M8CX-ray2.10A/B/C/D1-125[»]
3MHEX-ray1.72A/B1-125[»]
3MKIX-ray2.00A/B/C/D1-125[»]
3MYTX-ray1.96A/B/C/D1-125[»]
3NBRX-ray1.73A1-125[»]
3NHXX-ray1.59A1-125[»]
3NM2X-ray1.89A1-125[»]
3NUVX-ray1.76A/B1-125[»]
3NXJX-ray1.97A/B1-125[»]
3OV4X-ray1.83A/B/C/D1-125[»]
3T8UX-ray2.50A/B/C/D1-125[»]
3UNLX-ray2.52A/B/C/D1-125[»]
4L7KX-ray2.10A/B/C/D/E/F/G/H/I/J/K/O1-125[»]
5DREX-ray2.15A1-125[»]
8CHOX-ray2.30A1-125[»]
ProteinModelPortaliP00947.
SMRiP00947.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi688245.CtCNB1_1323.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105X1P. Bacteria.
ENOG411288K. LUCA.

Enzyme and pathway databases

BRENDAi5.3.3.1. 1590.
SABIO-RKP00947.

Miscellaneous databases

EvolutionaryTraceiP00947.

Family and domain databases

Gene3Di3.10.450.50. 1 hit.
InterProiIPR002075. NTF2.
IPR032710. NTF2-like_dom.
IPR011944. Steroid_delta5-4_isomerase.
[Graphical view]
PfamiPF02136. NTF2. 1 hit.
[Graphical view]
SUPFAMiSSF54427. SSF54427. 1 hit.
TIGRFAMsiTIGR02246. TIGR02246. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSDIS_COMTE
AccessioniPrimary (citable) accession number: P00947
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1991
Last modified: November 2, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.