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P00946 (MANA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannose-6-phosphate isomerase

EC=5.3.1.8
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name=PMI
Gene names
Name:manA
Synonyms:pmi
Ordered Locus Names:b1613, JW1605
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length391 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide.

Catalytic activity

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the mannose-6-phosphate isomerase type 1 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

minCP181963EBI-554045,EBI-554060

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 391391Mannose-6-phosphate isomerase
PRO_0000194230

Sites

Active site2741 By similarity
Metal binding971Zinc By similarity
Metal binding991Zinc By similarity
Metal binding1341Zinc By similarity
Metal binding2551Zinc By similarity

Amino acid modifications

Modified residue2801N6-acetyllysine Ref.6

Sequences

Sequence LengthMass (Da)Tools
P00946 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 669737512CADBCCD

FASTA39142,850
        10         20         30         40         50         60 
MQKLINSVQN YAWGSKTALT ELYGMENPSS QPMAELWMGA HPKSSSRVQN AAGDIVSLRD 

        70         80         90        100        110        120 
VIESDKSTLL GEAVAKRFGE LPFLFKVLCA AQPLSIQVHP NKHNSEIGFA KENAAGIPMD 

       130        140        150        160        170        180 
AAERNYKDPN HKPELVFALT PFLAMNAFRE FSEIVSLLQP VAGAHPAIAH FLQQPDAERL 

       190        200        210        220        230        240 
SELFASLLNM QGEEKSRALA ILKSALDSQQ GEPWQTIRLI SEFYPEDSGL FSPLLLNVVK 

       250        260        270        280        290        300 
LNPGEAMFLF AETPHAYLQG VALEVMANSD NVLRAGLTPK YIDIPELVAN VKFEAKPANQ 

       310        320        330        340        350        360 
LLTQPVKQGA ELDFPIPVDD FAFSLHDLSD KETTISQQSA AILFCVEGDA TLWKGSQQLQ 

       370        380        390 
LKPGESAFIA ANESPVTVKG HGRLARVYNK L 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence and transcriptional start point of the phosphomannose isomerase gene (manA) of Escherichia coli."
Miles J.S., Guest J.R.
Gene 32:41-48(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]Robison K., O'Keeffe T., Church G.M.
Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 378-391.
Strain: K12 / EMG2.
[6]"Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli."
Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y.
Mol. Cell. Proteomics 8:215-225(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-280, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: K12 / JW1106 and K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M15380 Genomic DNA. Translation: AAA24109.1.
U00096 Genomic DNA. Translation: AAC74685.1.
AP009048 Genomic DNA. Translation: BAA15361.1.
U35067 Genomic DNA. Translation: AAA79050.1.
PIRISECMP. A01172.
RefSeqNP_416130.3. NC_000913.3.
YP_489876.1. NC_007779.1.

3D structure databases

ProteinModelPortalP00946.
SMRP00946. Positions 1-391.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-10153N.
IntActP00946. 19 interactions.
MINTMINT-1241491.
STRING511145.b1613.

2D gel databases

SWISS-2DPAGEP00946.

Proteomic databases

PaxDbP00946.
PRIDEP00946.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74685; AAC74685; b1613.
BAA15361; BAA15361; BAA15361.
GeneID12931261.
944840.
KEGGecj:Y75_p1589.
eco:b1613.
PATRIC32118528. VBIEscCol129921_1684.

Organism-specific databases

EchoBASEEB0561.
EcoGeneEG10566. manA.

Phylogenomic databases

eggNOGCOG1482.
HOGENOMHOG000241278.
KOK01809.
OMAQGEPWDT.
OrthoDBEOG6130B2.
PhylomeDBP00946.

Enzyme and pathway databases

BioCycEcoCyc:MANNPISOM-MONOMER.
ECOL316407:JW1605-MONOMER.
MetaCyc:MANNPISOM-MONOMER.

Gene expression databases

GenevestigatorP00946.

Family and domain databases

Gene3D2.60.120.10. 3 hits.
InterProIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERPTHR10309. PTHR10309. 1 hit.
PfamPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSPR00714. MAN6PISMRASE.
SUPFAMSSF51182. SSF51182. 1 hit.
TIGRFAMsTIGR00218. manA. 1 hit.
PROSITEPS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP00946.

Entry information

Entry nameMANA_ECOLI
AccessionPrimary (citable) accession number: P00946
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 9, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene