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P00946

- MANA_ECOLI

UniProt

P00946 - MANA_ECOLI

Protein

Mannose-6-phosphate isomerase

Gene

manA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide.

    Catalytic activityi

    D-mannose 6-phosphate = D-fructose 6-phosphate.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi97 – 971ZincBy similarity
    Metal bindingi99 – 991ZincBy similarity
    Metal bindingi134 – 1341ZincBy similarity
    Metal bindingi255 – 2551ZincBy similarity
    Active sitei274 – 2741By similarity

    GO - Molecular functioni

    1. mannose-6-phosphate isomerase activity Source: EcoCyc
    2. protein binding Source: IntAct
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. colanic acid biosynthetic process Source: EcoCyc
    2. GDP-mannose biosynthetic process Source: EcoCyc
    3. mannose catabolic process Source: EcoCyc

    Keywords - Molecular functioni

    Isomerase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciEcoCyc:MANNPISOM-MONOMER.
    ECOL316407:JW1605-MONOMER.
    MetaCyc:MANNPISOM-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannose-6-phosphate isomerase (EC:5.3.1.8)
    Alternative name(s):
    Phosphohexomutase
    Phosphomannose isomerase
    Short name:
    PMI
    Gene namesi
    Name:manA
    Synonyms:pmi
    Ordered Locus Names:b1613, JW1605
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10566. manA.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 391391Mannose-6-phosphate isomerasePRO_0000194230Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei280 – 2801N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP00946.
    PRIDEiP00946.

    2D gel databases

    SWISS-2DPAGEP00946.

    Expressioni

    Gene expression databases

    GenevestigatoriP00946.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    minCP181963EBI-554045,EBI-554060

    Protein-protein interaction databases

    DIPiDIP-10153N.
    IntActiP00946. 19 interactions.
    MINTiMINT-1241491.
    STRINGi511145.b1613.

    Structurei

    3D structure databases

    ProteinModelPortaliP00946.
    SMRiP00946. Positions 1-391.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1482.
    HOGENOMiHOG000241278.
    KOiK01809.
    OMAiQGEPWDT.
    OrthoDBiEOG6130B2.
    PhylomeDBiP00946.

    Family and domain databases

    Gene3Di2.60.120.10. 3 hits.
    InterProiIPR001250. Man6P_Isoase-1.
    IPR016305. Mannose-6-P_Isomerase.
    IPR018050. Pmannose_isomerase-type1_CS.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view]
    PANTHERiPTHR10309. PTHR10309. 1 hit.
    PfamiPF01238. PMI_typeI. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
    PRINTSiPR00714. MAN6PISMRASE.
    SUPFAMiSSF51182. SSF51182. 1 hit.
    TIGRFAMsiTIGR00218. manA. 1 hit.
    PROSITEiPS00965. PMI_I_1. 1 hit.
    PS00966. PMI_I_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00946-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQKLINSVQN YAWGSKTALT ELYGMENPSS QPMAELWMGA HPKSSSRVQN    50
    AAGDIVSLRD VIESDKSTLL GEAVAKRFGE LPFLFKVLCA AQPLSIQVHP 100
    NKHNSEIGFA KENAAGIPMD AAERNYKDPN HKPELVFALT PFLAMNAFRE 150
    FSEIVSLLQP VAGAHPAIAH FLQQPDAERL SELFASLLNM QGEEKSRALA 200
    ILKSALDSQQ GEPWQTIRLI SEFYPEDSGL FSPLLLNVVK LNPGEAMFLF 250
    AETPHAYLQG VALEVMANSD NVLRAGLTPK YIDIPELVAN VKFEAKPANQ 300
    LLTQPVKQGA ELDFPIPVDD FAFSLHDLSD KETTISQQSA AILFCVEGDA 350
    TLWKGSQQLQ LKPGESAFIA ANESPVTVKG HGRLARVYNK L 391
    Length:391
    Mass (Da):42,850
    Last modified:July 21, 1986 - v1
    Checksum:i669737512CADBCCD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15380 Genomic DNA. Translation: AAA24109.1.
    U00096 Genomic DNA. Translation: AAC74685.1.
    AP009048 Genomic DNA. Translation: BAA15361.1.
    U35067 Genomic DNA. Translation: AAA79050.1.
    PIRiA01172. ISECMP.
    RefSeqiNP_416130.3. NC_000913.3.
    YP_489876.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74685; AAC74685; b1613.
    BAA15361; BAA15361; BAA15361.
    GeneIDi12931261.
    944840.
    KEGGiecj:Y75_p1589.
    eco:b1613.
    PATRICi32118528. VBIEscCol129921_1684.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15380 Genomic DNA. Translation: AAA24109.1 .
    U00096 Genomic DNA. Translation: AAC74685.1 .
    AP009048 Genomic DNA. Translation: BAA15361.1 .
    U35067 Genomic DNA. Translation: AAA79050.1 .
    PIRi A01172. ISECMP.
    RefSeqi NP_416130.3. NC_000913.3.
    YP_489876.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P00946.
    SMRi P00946. Positions 1-391.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10153N.
    IntActi P00946. 19 interactions.
    MINTi MINT-1241491.
    STRINGi 511145.b1613.

    2D gel databases

    SWISS-2DPAGE P00946.

    Proteomic databases

    PaxDbi P00946.
    PRIDEi P00946.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74685 ; AAC74685 ; b1613 .
    BAA15361 ; BAA15361 ; BAA15361 .
    GeneIDi 12931261.
    944840.
    KEGGi ecj:Y75_p1589.
    eco:b1613.
    PATRICi 32118528. VBIEscCol129921_1684.

    Organism-specific databases

    EchoBASEi EB0561.
    EcoGenei EG10566. manA.

    Phylogenomic databases

    eggNOGi COG1482.
    HOGENOMi HOG000241278.
    KOi K01809.
    OMAi QGEPWDT.
    OrthoDBi EOG6130B2.
    PhylomeDBi P00946.

    Enzyme and pathway databases

    BioCyci EcoCyc:MANNPISOM-MONOMER.
    ECOL316407:JW1605-MONOMER.
    MetaCyc:MANNPISOM-MONOMER.

    Miscellaneous databases

    PROi P00946.

    Gene expression databases

    Genevestigatori P00946.

    Family and domain databases

    Gene3Di 2.60.120.10. 3 hits.
    InterProi IPR001250. Man6P_Isoase-1.
    IPR016305. Mannose-6-P_Isomerase.
    IPR018050. Pmannose_isomerase-type1_CS.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view ]
    PANTHERi PTHR10309. PTHR10309. 1 hit.
    Pfami PF01238. PMI_typeI. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
    PRINTSi PR00714. MAN6PISMRASE.
    SUPFAMi SSF51182. SSF51182. 1 hit.
    TIGRFAMsi TIGR00218. manA. 1 hit.
    PROSITEi PS00965. PMI_I_1. 1 hit.
    PS00966. PMI_I_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and transcriptional start point of the phosphomannose isomerase gene (manA) of Escherichia coli."
      Miles J.S., Guest J.R.
      Gene 32:41-48(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. Robison K., O'Keeffe T., Church G.M.
      Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 378-391.
      Strain: K12 / EMG2.
    6. "Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli."
      Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y.
      Mol. Cell. Proteomics 8:215-225(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-280, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: K12 / JW1106 and K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiMANA_ECOLI
    AccessioniPrimary (citable) accession number: P00946
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3