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Protein

Mannose-6-phosphate isomerase

Gene

manA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide.

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi97 – 971ZincBy similarity
Metal bindingi99 – 991ZincBy similarity
Metal bindingi134 – 1341ZincBy similarity
Metal bindingi255 – 2551ZincBy similarity
Active sitei274 – 2741By similarity

GO - Molecular functioni

  • mannose-6-phosphate isomerase activity Source: EcoCyc
  • zinc ion binding Source: InterPro

GO - Biological processi

  • colanic acid biosynthetic process Source: EcoCyc
  • GDP-mannose biosynthetic process Source: EcoCyc
  • mannose catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:MANNPISOM-MONOMER.
ECOL316407:JW1605-MONOMER.
MetaCyc:MANNPISOM-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Name:manA
Synonyms:pmi
Ordered Locus Names:b1613, JW1605
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10566. manA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 391391Mannose-6-phosphate isomerasePRO_0000194230Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei280 – 2801N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP00946.
PRIDEiP00946.

2D gel databases

SWISS-2DPAGEP00946.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
minCP181963EBI-554045,EBI-554060

Protein-protein interaction databases

DIPiDIP-10153N.
IntActiP00946. 19 interactions.
MINTiMINT-1241491.
STRINGi511145.b1613.

Structurei

3D structure databases

ProteinModelPortaliP00946.
SMRiP00946. Positions 1-391.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1482.
HOGENOMiHOG000241278.
InParanoidiP00946.
KOiK01809.
OMAiVMKMEVP.
OrthoDBiEOG6130B2.
PhylomeDBiP00946.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00946-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKLINSVQN YAWGSKTALT ELYGMENPSS QPMAELWMGA HPKSSSRVQN
60 70 80 90 100
AAGDIVSLRD VIESDKSTLL GEAVAKRFGE LPFLFKVLCA AQPLSIQVHP
110 120 130 140 150
NKHNSEIGFA KENAAGIPMD AAERNYKDPN HKPELVFALT PFLAMNAFRE
160 170 180 190 200
FSEIVSLLQP VAGAHPAIAH FLQQPDAERL SELFASLLNM QGEEKSRALA
210 220 230 240 250
ILKSALDSQQ GEPWQTIRLI SEFYPEDSGL FSPLLLNVVK LNPGEAMFLF
260 270 280 290 300
AETPHAYLQG VALEVMANSD NVLRAGLTPK YIDIPELVAN VKFEAKPANQ
310 320 330 340 350
LLTQPVKQGA ELDFPIPVDD FAFSLHDLSD KETTISQQSA AILFCVEGDA
360 370 380 390
TLWKGSQQLQ LKPGESAFIA ANESPVTVKG HGRLARVYNK L
Length:391
Mass (Da):42,850
Last modified:July 21, 1986 - v1
Checksum:i669737512CADBCCD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15380 Genomic DNA. Translation: AAA24109.1.
U00096 Genomic DNA. Translation: AAC74685.1.
AP009048 Genomic DNA. Translation: BAA15361.1.
U35067 Genomic DNA. Translation: AAA79050.1.
PIRiA01172. ISECMP.
RefSeqiNP_416130.3. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74685; AAC74685; b1613.
BAA15361; BAA15361; BAA15361.
GeneIDi944840.
KEGGiecj:Y75_p1589.
eco:b1613.
PATRICi32118528. VBIEscCol129921_1684.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15380 Genomic DNA. Translation: AAA24109.1.
U00096 Genomic DNA. Translation: AAC74685.1.
AP009048 Genomic DNA. Translation: BAA15361.1.
U35067 Genomic DNA. Translation: AAA79050.1.
PIRiA01172. ISECMP.
RefSeqiNP_416130.3. NC_000913.3.

3D structure databases

ProteinModelPortaliP00946.
SMRiP00946. Positions 1-391.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-10153N.
IntActiP00946. 19 interactions.
MINTiMINT-1241491.
STRINGi511145.b1613.

2D gel databases

SWISS-2DPAGEP00946.

Proteomic databases

PaxDbiP00946.
PRIDEiP00946.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74685; AAC74685; b1613.
BAA15361; BAA15361; BAA15361.
GeneIDi944840.
KEGGiecj:Y75_p1589.
eco:b1613.
PATRICi32118528. VBIEscCol129921_1684.

Organism-specific databases

EchoBASEiEB0561.
EcoGeneiEG10566. manA.

Phylogenomic databases

eggNOGiCOG1482.
HOGENOMiHOG000241278.
InParanoidiP00946.
KOiK01809.
OMAiVMKMEVP.
OrthoDBiEOG6130B2.
PhylomeDBiP00946.

Enzyme and pathway databases

BioCyciEcoCyc:MANNPISOM-MONOMER.
ECOL316407:JW1605-MONOMER.
MetaCyc:MANNPISOM-MONOMER.

Miscellaneous databases

PROiP00946.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and transcriptional start point of the phosphomannose isomerase gene (manA) of Escherichia coli."
    Miles J.S., Guest J.R.
    Gene 32:41-48(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. Robison K., O'Keeffe T., Church G.M.
    Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 378-391.
    Strain: K12 / EMG2.
  6. "Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli."
    Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y.
    Mol. Cell. Proteomics 8:215-225(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-280, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: K12 / JW1106 and K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiMANA_ECOLI
AccessioniPrimary (citable) accession number: P00946
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 24, 2015
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.