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P00946

- MANA_ECOLI

UniProt

P00946 - MANA_ECOLI

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Protein

Mannose-6-phosphate isomerase

Gene

manA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide.

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi97 – 971ZincBy similarity
Metal bindingi99 – 991ZincBy similarity
Metal bindingi134 – 1341ZincBy similarity
Metal bindingi255 – 2551ZincBy similarity
Active sitei274 – 2741By similarity

GO - Molecular functioni

  1. mannose-6-phosphate isomerase activity Source: EcoCyc
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. colanic acid biosynthetic process Source: EcoCyc
  2. GDP-mannose biosynthetic process Source: EcoCyc
  3. mannose catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:MANNPISOM-MONOMER.
ECOL316407:JW1605-MONOMER.
MetaCyc:MANNPISOM-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Name:manA
Synonyms:pmi
Ordered Locus Names:b1613, JW1605
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10566. manA.

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 391391Mannose-6-phosphate isomerasePRO_0000194230Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei280 – 2801N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP00946.
PRIDEiP00946.

2D gel databases

SWISS-2DPAGEP00946.

Expressioni

Gene expression databases

GenevestigatoriP00946.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
minCP181963EBI-554045,EBI-554060

Protein-protein interaction databases

DIPiDIP-10153N.
IntActiP00946. 19 interactions.
MINTiMINT-1241491.
STRINGi511145.b1613.

Structurei

3D structure databases

ProteinModelPortaliP00946.
SMRiP00946. Positions 1-391.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1482.
HOGENOMiHOG000241278.
InParanoidiP00946.
KOiK01809.
OMAiQGEPWDT.
OrthoDBiEOG6130B2.
PhylomeDBiP00946.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00946-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQKLINSVQN YAWGSKTALT ELYGMENPSS QPMAELWMGA HPKSSSRVQN
60 70 80 90 100
AAGDIVSLRD VIESDKSTLL GEAVAKRFGE LPFLFKVLCA AQPLSIQVHP
110 120 130 140 150
NKHNSEIGFA KENAAGIPMD AAERNYKDPN HKPELVFALT PFLAMNAFRE
160 170 180 190 200
FSEIVSLLQP VAGAHPAIAH FLQQPDAERL SELFASLLNM QGEEKSRALA
210 220 230 240 250
ILKSALDSQQ GEPWQTIRLI SEFYPEDSGL FSPLLLNVVK LNPGEAMFLF
260 270 280 290 300
AETPHAYLQG VALEVMANSD NVLRAGLTPK YIDIPELVAN VKFEAKPANQ
310 320 330 340 350
LLTQPVKQGA ELDFPIPVDD FAFSLHDLSD KETTISQQSA AILFCVEGDA
360 370 380 390
TLWKGSQQLQ LKPGESAFIA ANESPVTVKG HGRLARVYNK L
Length:391
Mass (Da):42,850
Last modified:July 21, 1986 - v1
Checksum:i669737512CADBCCD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15380 Genomic DNA. Translation: AAA24109.1.
U00096 Genomic DNA. Translation: AAC74685.1.
AP009048 Genomic DNA. Translation: BAA15361.1.
U35067 Genomic DNA. Translation: AAA79050.1.
PIRiA01172. ISECMP.
RefSeqiNP_416130.3. NC_000913.3.
YP_489876.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74685; AAC74685; b1613.
BAA15361; BAA15361; BAA15361.
GeneIDi12931261.
944840.
KEGGiecj:Y75_p1589.
eco:b1613.
PATRICi32118528. VBIEscCol129921_1684.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15380 Genomic DNA. Translation: AAA24109.1 .
U00096 Genomic DNA. Translation: AAC74685.1 .
AP009048 Genomic DNA. Translation: BAA15361.1 .
U35067 Genomic DNA. Translation: AAA79050.1 .
PIRi A01172. ISECMP.
RefSeqi NP_416130.3. NC_000913.3.
YP_489876.1. NC_007779.1.

3D structure databases

ProteinModelPortali P00946.
SMRi P00946. Positions 1-391.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-10153N.
IntActi P00946. 19 interactions.
MINTi MINT-1241491.
STRINGi 511145.b1613.

2D gel databases

SWISS-2DPAGE P00946.

Proteomic databases

PaxDbi P00946.
PRIDEi P00946.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74685 ; AAC74685 ; b1613 .
BAA15361 ; BAA15361 ; BAA15361 .
GeneIDi 12931261.
944840.
KEGGi ecj:Y75_p1589.
eco:b1613.
PATRICi 32118528. VBIEscCol129921_1684.

Organism-specific databases

EchoBASEi EB0561.
EcoGenei EG10566. manA.

Phylogenomic databases

eggNOGi COG1482.
HOGENOMi HOG000241278.
InParanoidi P00946.
KOi K01809.
OMAi QGEPWDT.
OrthoDBi EOG6130B2.
PhylomeDBi P00946.

Enzyme and pathway databases

BioCyci EcoCyc:MANNPISOM-MONOMER.
ECOL316407:JW1605-MONOMER.
MetaCyc:MANNPISOM-MONOMER.

Miscellaneous databases

PROi P00946.

Gene expression databases

Genevestigatori P00946.

Family and domain databases

Gene3Di 2.60.120.10. 3 hits.
InterProi IPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view ]
PANTHERi PTHR10309. PTHR10309. 1 hit.
Pfami PF01238. PMI_typeI. 1 hit.
[Graphical view ]
PIRSFi PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSi PR00714. MAN6PISMRASE.
SUPFAMi SSF51182. SSF51182. 1 hit.
TIGRFAMsi TIGR00218. manA. 1 hit.
PROSITEi PS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and transcriptional start point of the phosphomannose isomerase gene (manA) of Escherichia coli."
    Miles J.S., Guest J.R.
    Gene 32:41-48(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. Robison K., O'Keeffe T., Church G.M.
    Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 378-391.
    Strain: K12 / EMG2.
  6. "Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli."
    Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y.
    Mol. Cell. Proteomics 8:215-225(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-280, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: K12 / JW1106 and K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiMANA_ECOLI
AccessioniPrimary (citable) accession number: P00946
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 26, 2014
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3