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Protein

Xylose isomerase

Gene

xylA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

D-xylopyranose = D-xylulose.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei101 – 10111 Publication
Active sitei104 – 1041By similarity
Metal bindingi232 – 2321Magnesium 1By similarity
Metal bindingi268 – 2681Magnesium 1By similarity
Metal bindingi268 – 2681Magnesium 2By similarity
Metal bindingi271 – 2711Magnesium 2By similarity
Metal bindingi296 – 2961Magnesium 1By similarity
Metal bindingi307 – 3071Magnesium 2By similarity
Metal bindingi309 – 3091Magnesium 2By similarity
Metal bindingi339 – 3391Magnesium 1By similarity

GO - Molecular functioni

  • magnesium ion binding Source: UniProtKB-HAMAP
  • xylose isomerase activity Source: CACAO

GO - Biological processi

  • D-xylose catabolic process Source: EcoCyc
  • pentose-phosphate shunt Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism, Pentose shunt, Xylose metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:XYLISOM-MONOMER.
ECOL316407:JW3537-MONOMER.
MetaCyc:XYLISOM-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Xylose isomerase (EC:5.3.1.5)
Alternative name(s):
D-xylulose keto-isomerase
Gene namesi
Name:xylA
Ordered Locus Names:b3565, JW3537
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11074. xylA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440Xylose isomerasePRO_0000195774Add
BLAST

Proteomic databases

EPDiP00944.
PaxDbiP00944.
PRIDEiP00944.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi4262540. 14 interactions.
DIPiDIP-11149N.
IntActiP00944. 8 interactions.
MINTiMINT-1238504.
STRINGi511145.b3565.

Structurei

3D structure databases

ProteinModelPortaliP00944.
SMRiP00944. Positions 4-439.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the xylose isomerase family.Curated

Phylogenomic databases

eggNOGiENOG4105C93. Bacteria.
COG2115. LUCA.
HOGENOMiHOG000252293.
InParanoidiP00944.
KOiK01805.
OMAiHTFQHEL.
OrthoDBiEOG62NX4R.
PhylomeDBiP00944.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00455. Xylose_isom_A.
InterProiIPR013022. Xyl_isomerase-like_TIM-brl.
IPR013452. Xylose_isom_bac.
IPR001998. Xylose_isomerase.
[Graphical view]
PRINTSiPR00688. XYLOSISMRASE.
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR02630. xylose_isom_A. 1 hit.
PROSITEiPS51415. XYLOSE_ISOMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00944-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAYFDQLDR VRYEGSKSSN PLAFRHYNPD ELVLGKRMEE HLRFAACYWH
60 70 80 90 100
TFCWNGADMF GVGAFNRPWQ QPGEALALAK RKADVAFEFF HKLHVPFYCF
110 120 130 140 150
HDVDVSPEGA SLKEYINNFA QMVDVLAGKQ EESGVKLLWG TANCFTNPRY
160 170 180 190 200
GAGAATNPDP EVFSWAATQV VTAMEATHKL GGENYVLWGG REGYETLLNT
210 220 230 240 250
DLRQEREQLG RFMQMVVEHK HKIGFQGTLL IEPKPQEPTK HQYDYDAATV
260 270 280 290 300
YGFLKQFGLE KEIKLNIEAN HATLAGHSFH HEIATAIALG LFGSVDANRG
310 320 330 340 350
DAQLGWDTDQ FPNSVEENAL VMYEILKAGG FTTGGLNFDA KVRRQSTDKY
360 370 380 390 400
DLFYGHIGAM DTMALALKIA ARMIEDGELD KRIAQRYSGW NSELGQQILK
410 420 430 440
GQMSLADLAK YAQEHHLSPV HQSGRQEQLE NLVNHYLFDK
Length:440
Mass (Da):49,742
Last modified:July 21, 1986 - v1
Checksum:i6841659B6EC0561F
GO

Sequence cautioni

The sequence CAA25347.1 differs from that shown. Reason: Frameshift at position 67. Curated
The sequence CAA28394.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti169 – 1691Q → E in AAB20471 (PubMed:2132127).Curated
Sequence conflicti299 – 2991R → L in AAB20471 (PubMed:2132127).Curated
Sequence conflicti342 – 3421V → D in AAB20471 (PubMed:2132127).Curated
Sequence conflicti378 – 3781E → D in AAB20471 (PubMed:2132127).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01996 Genomic DNA. Translation: AAA24768.1.
X04691 Genomic DNA. Translation: CAA28394.1. Different initiation.
X00772 Genomic DNA. Translation: CAA25346.1. Frameshift.
X00772 Genomic DNA. Translation: CAA25347.1. Frameshift.
S68257 Genomic DNA. Translation: AAB20471.2.
U00039 Genomic DNA. Translation: AAB18542.1.
U00096 Genomic DNA. Translation: AAC76589.1.
AP009048 Genomic DNA. Translation: BAE77728.1.
PIRiA30252. ISECX1.
RefSeqiNP_418022.1. NC_000913.3.
WP_001149591.1. NZ_CP014272.1.

Genome annotation databases

EnsemblBacteriaiAAC76589; AAC76589; b3565.
BAE77728; BAE77728; BAE77728.
GeneIDi948141.
KEGGiecj:JW3537.
eco:b3565.
PATRICi32122606. VBIEscCol129921_3680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01996 Genomic DNA. Translation: AAA24768.1.
X04691 Genomic DNA. Translation: CAA28394.1. Different initiation.
X00772 Genomic DNA. Translation: CAA25346.1. Frameshift.
X00772 Genomic DNA. Translation: CAA25347.1. Frameshift.
S68257 Genomic DNA. Translation: AAB20471.2.
U00039 Genomic DNA. Translation: AAB18542.1.
U00096 Genomic DNA. Translation: AAC76589.1.
AP009048 Genomic DNA. Translation: BAE77728.1.
PIRiA30252. ISECX1.
RefSeqiNP_418022.1. NC_000913.3.
WP_001149591.1. NZ_CP014272.1.

3D structure databases

ProteinModelPortaliP00944.
SMRiP00944. Positions 4-439.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262540. 14 interactions.
DIPiDIP-11149N.
IntActiP00944. 8 interactions.
MINTiMINT-1238504.
STRINGi511145.b3565.

Proteomic databases

EPDiP00944.
PaxDbiP00944.
PRIDEiP00944.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76589; AAC76589; b3565.
BAE77728; BAE77728; BAE77728.
GeneIDi948141.
KEGGiecj:JW3537.
eco:b3565.
PATRICi32122606. VBIEscCol129921_3680.

Organism-specific databases

EchoBASEiEB1067.
EcoGeneiEG11074. xylA.

Phylogenomic databases

eggNOGiENOG4105C93. Bacteria.
COG2115. LUCA.
HOGENOMiHOG000252293.
InParanoidiP00944.
KOiK01805.
OMAiHTFQHEL.
OrthoDBiEOG62NX4R.
PhylomeDBiP00944.

Enzyme and pathway databases

BioCyciEcoCyc:XYLISOM-MONOMER.
ECOL316407:JW3537-MONOMER.
MetaCyc:XYLISOM-MONOMER.

Miscellaneous databases

PROiP00944.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00455. Xylose_isom_A.
InterProiIPR013022. Xyl_isomerase-like_TIM-brl.
IPR013452. Xylose_isom_bac.
IPR001998. Xylose_isomerase.
[Graphical view]
PRINTSiPR00688. XYLOSISMRASE.
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR02630. xylose_isom_A. 1 hit.
PROSITEiPS51415. XYLOSE_ISOMERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of the xylose isomerase and xylulose kinase genes of Escherichia coli."
    Lawlis V.B., Dennis M.S., Chen E.Y., Smith D.H., Henner D.J.
    Appl. Environ. Microbiol. 47:15-21(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / MM294 / ATCC 33625 / DSM 5208.
  2. "Xylose isomerase from Escherichia coli. Characterization of the protein and the structural gene."
    Schellenberg G.D., Sarthy A., Larson A.E., Backer M.P., Crabb J.W., Lidstrom M., Hall B.D., Furlong C.E.
    J. Biol. Chem. 259:6826-6832(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Molecular cloning, DNA structure and expression of the Escherichia coli D-xylose isomerase."
    Briggs K.A., Lancashire W.E., Hartley B.S.
    EMBO J. 3:611-616(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Sequence analysis of D-xylose isomerase gene from Escherichia coli."
    Hou Y.M., Zhang Q.J., Wang S.J.
    Chin. J. Biotechnol. 6:269-277(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  8. "Identification of essential histidine residues in the active site of Escherichia coli xylose (glucose) isomerase."
    Batt C.A., Jamieson A.C., Vandeyar M.A.
    Proc. Natl. Acad. Sci. U.S.A. 87:618-622(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVE SITE.

Entry informationi

Entry nameiXYLA_ECOLI
AccessioniPrimary (citable) accession number: P00944
Secondary accession number(s): P00945, Q2M7M8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 11, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.