Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Triosephosphate isomerase

Gene

TPI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Triosephosphate isomerase (TPI1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10Substrate1 Publication1
Binding sitei12Substrate1 Publication1
Active sitei95Electrophile1
Active sitei165Proton acceptor1

GO - Molecular functioni

  • triose-phosphate isomerase activity Source: SGD

GO - Biological processi

  • gluconeogenesis Source: GO_Central
  • glyceraldehyde-3-phosphate biosynthetic process Source: GO_Central
  • glycerol catabolic process Source: GO_Central
  • glycolytic process Source: SGD
  • pentose-phosphate shunt Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis, Pentose shunt

Enzyme and pathway databases

BioCyciYEAST:YDR050C-MONOMER.
BRENDAi5.3.1.1. 984.
ReactomeiR-SCE-70171. Glycolysis.
R-SCE-70263. Gluconeogenesis.
SABIO-RKP00942.
UniPathwayiUPA00109; UER00189.
UPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomerase (EC:5.3.1.1)
Short name:
TIM
Alternative name(s):
Triose-phosphate isomerase
Gene namesi
Name:TPI1
Ordered Locus Names:YDR050C
ORF Names:YD9609.05C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR050C.
SGDiS000002457. TPI1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytosol Source: GO_Central
  • mitochondrion Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000901692 – 248Triosephosphate isomeraseAdd BLAST247

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphothreonineCombined sources1
Modified residuei71PhosphoserineCombined sources1
Modified residuei215PhosphoserineCombined sources1
Cross-linki223Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP00942.
PRIDEiP00942.
TopDownProteomicsiP00942.

2D gel databases

COMPLUYEAST-2DPAGEP00942.
SWISS-2DPAGEP00942.

PTM databases

iPTMnetiP00942.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi32104. 51 interactors.
DIPiDIP-6671N.
IntActiP00942. 10 interactors.
MINTiMINT-614733.

Structurei

Secondary structure

1248
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 10Combined sources6
Helixi17 – 29Combined sources13
Beta strandi36 – 41Combined sources6
Helixi44 – 46Combined sources3
Helixi47 – 53Combined sources7
Beta strandi59 – 64Combined sources6
Beta strandi68 – 73Combined sources6
Helixi80 – 85Combined sources6
Beta strandi90 – 94Combined sources5
Helixi96 – 100Combined sources5
Helixi106 – 118Combined sources13
Beta strandi122 – 127Combined sources6
Helixi131 – 135Combined sources5
Helixi139 – 153Combined sources15
Beta strandi160 – 164Combined sources5
Helixi167 – 169Combined sources3
Turni170 – 172Combined sources3
Helixi178 – 196Combined sources19
Helixi198 – 203Combined sources6
Beta strandi206 – 211Combined sources6
Turni214 – 216Combined sources3
Helixi217 – 220Combined sources4
Beta strandi228 – 232Combined sources5
Helixi233 – 236Combined sources4
Helixi239 – 244Combined sources6
Turni245 – 247Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I45X-ray1.80A/B2-248[»]
1NEYX-ray1.20A/B2-248[»]
1NF0X-ray1.60A/B2-248[»]
1YPIX-ray1.90A/B2-248[»]
2YPIX-ray2.50A/B2-248[»]
3YPIX-ray2.80A/B2-248[»]
4FF7X-ray1.86A/B1-248[»]
7TIMX-ray1.90A/B2-248[»]
DisProtiDP00430.
ProteinModelPortaliP00942.
SMRiP00942.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00942.

Family & Domainsi

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000013354.
HOGENOMiHOG000226413.
InParanoidiP00942.
KOiK01803.
OMAiHRFRIHP.
OrthoDBiEOG092C484E.

Family and domain databases

CDDicd00311. TIM. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00942-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARTFFVGGN FKLNGSKQSI KEIVERLNTA SIPENVEVVI CPPATYLDYS
60 70 80 90 100
VSLVKKPQVT VGAQNAYLKA SGAFTGENSV DQIKDVGAKW VILGHSERRS
110 120 130 140 150
YFHEDDKFIA DKTKFALGQG VGVILCIGET LEEKKAGKTL DVVERQLNAV
160 170 180 190 200
LEEVKDWTNV VVAYEPVWAI GTGLAATPED AQDIHASIRK FLASKLGDKA
210 220 230 240
ASELRILYGG SANGSNAVTF KDKADVDGFL VGGASLKPEF VDIINSRN
Length:248
Mass (Da):26,795
Last modified:January 23, 2007 - v2
Checksum:i8429251C4DF51F86
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01366 Genomic DNA. Translation: AAA88757.1.
Z49209 Genomic DNA. Translation: CAA89080.1.
AY557654 Genomic DNA. Translation: AAS55980.1.
BK006938 Genomic DNA. Translation: DAA11897.1.
PIRiA01168. ISBYT.
RefSeqiNP_010335.1. NM_001180358.1.

Genome annotation databases

EnsemblFungiiYDR050C; YDR050C; YDR050C.
GeneIDi851620.
KEGGisce:YDR050C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01366 Genomic DNA. Translation: AAA88757.1.
Z49209 Genomic DNA. Translation: CAA89080.1.
AY557654 Genomic DNA. Translation: AAS55980.1.
BK006938 Genomic DNA. Translation: DAA11897.1.
PIRiA01168. ISBYT.
RefSeqiNP_010335.1. NM_001180358.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I45X-ray1.80A/B2-248[»]
1NEYX-ray1.20A/B2-248[»]
1NF0X-ray1.60A/B2-248[»]
1YPIX-ray1.90A/B2-248[»]
2YPIX-ray2.50A/B2-248[»]
3YPIX-ray2.80A/B2-248[»]
4FF7X-ray1.86A/B1-248[»]
7TIMX-ray1.90A/B2-248[»]
DisProtiDP00430.
ProteinModelPortaliP00942.
SMRiP00942.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32104. 51 interactors.
DIPiDIP-6671N.
IntActiP00942. 10 interactors.
MINTiMINT-614733.

PTM databases

iPTMnetiP00942.

2D gel databases

COMPLUYEAST-2DPAGEP00942.
SWISS-2DPAGEP00942.

Proteomic databases

MaxQBiP00942.
PRIDEiP00942.
TopDownProteomicsiP00942.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR050C; YDR050C; YDR050C.
GeneIDi851620.
KEGGisce:YDR050C.

Organism-specific databases

EuPathDBiFungiDB:YDR050C.
SGDiS000002457. TPI1.

Phylogenomic databases

GeneTreeiENSGT00390000013354.
HOGENOMiHOG000226413.
InParanoidiP00942.
KOiK01803.
OMAiHRFRIHP.
OrthoDBiEOG092C484E.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00189.
UPA00138.
BioCyciYEAST:YDR050C-MONOMER.
BRENDAi5.3.1.1. 984.
ReactomeiR-SCE-70171. Glycolysis.
R-SCE-70263. Gluconeogenesis.
SABIO-RKP00942.

Miscellaneous databases

EvolutionaryTraceiP00942.
PROiP00942.

Family and domain databases

CDDicd00311. TIM. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPIS_YEAST
AccessioniPrimary (citable) accession number: P00942
Secondary accession number(s): D6VS37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 179 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 207000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.