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Protein

Triosephosphate isomerase

Gene

TPI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 207000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Triosephosphate isomerase (TPI1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10Substrate1 Publication1
Binding sitei12Substrate1 Publication1
Active sitei95Electrophile1
Active sitei165Proton acceptor1

GO - Molecular functioni

  • triose-phosphate isomerase activity Source: SGD

GO - Biological processi

  • gluconeogenesis Source: GO_Central
  • glyceraldehyde-3-phosphate biosynthetic process Source: GO_Central
  • glycerol catabolic process Source: GO_Central
  • glycolytic process Source: SGD

Keywordsi

Molecular functionIsomerase
Biological processGluconeogenesis, Glycolysis

Enzyme and pathway databases

BioCyciYEAST:YDR050C-MONOMER
BRENDAi5.3.1.1 984
ReactomeiR-SCE-70171 Glycolysis
R-SCE-70263 Gluconeogenesis
SABIO-RKiP00942
UniPathwayiUPA00109; UER00189
UPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomerase (EC:5.3.1.1)
Short name:
TIM
Alternative name(s):
Triose-phosphate isomerase
Gene namesi
Name:TPI1
Ordered Locus Names:YDR050C
ORF Names:YD9609.05C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR050C
SGDiS000002457 TPI1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000901692 – 248Triosephosphate isomeraseAdd BLAST247

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphothreonineCombined sources1
Modified residuei71PhosphoserineCombined sources1
Modified residuei215PhosphoserineCombined sources1
Cross-linki223Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP00942
PaxDbiP00942
PRIDEiP00942
TopDownProteomicsiP00942

2D gel databases

COMPLUYEAST-2DPAGEiP00942
SWISS-2DPAGEiP00942

PTM databases

iPTMnetiP00942

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi32104, 123 interactors
DIPiDIP-6671N
IntActiP00942, 18 interactors
MINTiP00942
STRINGi4932.YDR050C

Structurei

Secondary structure

1248
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 10Combined sources6
Helixi17 – 29Combined sources13
Beta strandi36 – 41Combined sources6
Helixi44 – 46Combined sources3
Helixi47 – 53Combined sources7
Beta strandi59 – 64Combined sources6
Beta strandi68 – 73Combined sources6
Helixi80 – 85Combined sources6
Beta strandi90 – 94Combined sources5
Helixi96 – 100Combined sources5
Helixi106 – 118Combined sources13
Beta strandi122 – 127Combined sources6
Helixi131 – 135Combined sources5
Helixi139 – 153Combined sources15
Beta strandi160 – 164Combined sources5
Helixi167 – 169Combined sources3
Turni170 – 172Combined sources3
Helixi178 – 196Combined sources19
Helixi198 – 203Combined sources6
Beta strandi206 – 211Combined sources6
Turni214 – 216Combined sources3
Helixi217 – 220Combined sources4
Beta strandi228 – 232Combined sources5
Helixi233 – 236Combined sources4
Helixi239 – 244Combined sources6
Turni245 – 247Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I45X-ray1.80A/B2-248[»]
1NEYX-ray1.20A/B2-248[»]
1NF0X-ray1.60A/B2-248[»]
1YPIX-ray1.90A/B2-248[»]
2YPIX-ray2.50A/B2-248[»]
3YPIX-ray2.80A/B2-248[»]
4FF7X-ray1.86A/B1-248[»]
7TIMX-ray1.90A/B2-248[»]
ProteinModelPortaliP00942
SMRiP00942
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00942

Family & Domainsi

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000013354
HOGENOMiHOG000226413
InParanoidiP00942
KOiK01803
OMAiLCVGEGL
OrthoDBiEOG092C484E

Family and domain databases

CDDicd00311 TIM, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00147_B TIM_B, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR035990 TIM_sf
IPR022896 TrioseP_Isoase_bac/euk
IPR000652 Triosephosphate_isomerase
IPR020861 Triosephosphate_isomerase_AS
PANTHERiPTHR21139 PTHR21139, 1 hit
PfamiView protein in Pfam
PF00121 TIM, 1 hit
SUPFAMiSSF51351 SSF51351, 1 hit
TIGRFAMsiTIGR00419 tim, 1 hit
PROSITEiView protein in PROSITE
PS00171 TIM_1, 1 hit
PS51440 TIM_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00942-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARTFFVGGN FKLNGSKQSI KEIVERLNTA SIPENVEVVI CPPATYLDYS
60 70 80 90 100
VSLVKKPQVT VGAQNAYLKA SGAFTGENSV DQIKDVGAKW VILGHSERRS
110 120 130 140 150
YFHEDDKFIA DKTKFALGQG VGVILCIGET LEEKKAGKTL DVVERQLNAV
160 170 180 190 200
LEEVKDWTNV VVAYEPVWAI GTGLAATPED AQDIHASIRK FLASKLGDKA
210 220 230 240
ASELRILYGG SANGSNAVTF KDKADVDGFL VGGASLKPEF VDIINSRN
Length:248
Mass (Da):26,795
Last modified:January 23, 2007 - v2
Checksum:i8429251C4DF51F86
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01366 Genomic DNA Translation: AAA88757.1
Z49209 Genomic DNA Translation: CAA89080.1
AY557654 Genomic DNA Translation: AAS55980.1
BK006938 Genomic DNA Translation: DAA11897.1
PIRiA01168 ISBYT
RefSeqiNP_010335.1, NM_001180358.1

Genome annotation databases

EnsemblFungiiYDR050C; YDR050C; YDR050C
GeneIDi851620
KEGGisce:YDR050C

Similar proteinsi

Entry informationi

Entry nameiTPIS_YEAST
AccessioniPrimary (citable) accession number: P00942
Secondary accession number(s): D6VS37
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 193 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health