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Protein

Triosephosphate isomerase

Gene

TPI1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Triosephosphate isomerase (TPI1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13Substrate1
Active sitei95Electrophile1
Active sitei165Proton acceptor1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis, Pentose shunt

Enzyme and pathway databases

BRENDAi5.3.1.1. 1306.
ReactomeiR-GGA-352875. Gluconeogenesis.
R-GGA-352882. Glycolysis.
R-GGA-70171. Glycolysis.
R-GGA-70263. Gluconeogenesis.
SABIO-RKP00940.
UniPathwayiUPA00109; UER00189.
UPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomerase (EC:5.3.1.1)
Short name:
TIM
Alternative name(s):
Triose-phosphate isomerase
Gene namesi
Name:TPI1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi95H → N: Reduces activity 5000-fold. 1 Publication1
Mutagenesisi165E → D: Reduces activity 300-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000901212 – 248Triosephosphate isomeraseAdd BLAST247

Proteomic databases

PaxDbiP00940.
PRIDEiP00940.

Expressioni

Gene expression databases

BgeeiENSGALG00000014526.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi676684. 1 interactor.
IntActiP00940. 1 interactor.
STRINGi9031.ENSGALP00000023396.

Structurei

Secondary structure

1248
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 11Combined sources6
Helixi18 – 30Combined sources13
Beta strandi37 – 43Combined sources7
Helixi45 – 47Combined sources3
Helixi48 – 54Combined sources7
Beta strandi59 – 64Combined sources6
Beta strandi68 – 73Combined sources6
Helixi80 – 85Combined sources6
Beta strandi90 – 94Combined sources5
Helixi96 – 100Combined sources5
Helixi106 – 118Combined sources13
Beta strandi122 – 127Combined sources6
Helixi131 – 136Combined sources6
Helixi139 – 151Combined sources13
Helixi157 – 159Combined sources3
Beta strandi160 – 164Combined sources5
Helixi167 – 169Combined sources3
Beta strandi170 – 173Combined sources4
Helixi178 – 195Combined sources18
Helixi198 – 203Combined sources6
Beta strandi206 – 208Combined sources3
Turni214 – 216Combined sources3
Helixi217 – 221Combined sources5
Beta strandi228 – 232Combined sources5
Helixi233 – 236Combined sources4
Helixi240 – 244Combined sources5
Turni245 – 247Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SPQX-ray2.16A/B2-248[»]
1SQ7X-ray2.85A/B2-248[»]
1SSDX-ray2.90A/B2-248[»]
1SSGX-ray2.90A/B2-248[»]
1SU5X-ray2.70A/B2-248[»]
1SW0X-ray1.71A/B1-248[»]
1SW3X-ray2.03A/B1-248[»]
1SW7X-ray2.22A/B1-248[»]
1TIMX-ray2.50A/B2-248[»]
1TPBX-ray1.901/22-248[»]
1TPCX-ray1.901/22-248[»]
1TPHX-ray1.801/22-248[»]
1TPUX-ray1.90A/B2-248[»]
1TPVX-ray1.90A/B2-248[»]
1TPWX-ray1.90A/B2-248[»]
4P61X-ray1.34A/B1-248[»]
8TIMX-ray2.50A/B2-248[»]
ProteinModelPortaliP00940.
SMRiP00940.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00940.

Family & Domainsi

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.Curated

Phylogenomic databases

eggNOGiKOG1643. Eukaryota.
COG0149. LUCA.
GeneTreeiENSGT00390000013354.
HOGENOMiHOG000226413.
HOVERGENiHBG002599.
InParanoidiP00940.
KOiK01803.
OMAiIEKNGTM.
OrthoDBiEOG091G0LEZ.
PhylomeDBiP00940.
TreeFamiTF300829.

Family and domain databases

CDDicd00311. TIM. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00940-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPRKFFVGG NWKMNGDKKS LGELIHTLNG AKLSADTEVV CGAPSIYLDF
60 70 80 90 100
ARQKLDAKIG VAAQNCYKVP KGAFTGEISP AMIKDIGAAW VILGHSERRH
110 120 130 140 150
VFGESDELIG QKVAHALAEG LGVIACIGEK LDEREAGITE KVVFEQTKAI
160 170 180 190 200
ADNVKDWSKV VLAYEPVWAI GTGKTATPQQ AQEVHEKLRG WLKSHVSDAV
210 220 230 240
AQSTRIIYGG SVTGGNCKEL ASQHDVDGFL VGGASLKPEF VDIINAKH
Length:248
Mass (Da):26,620
Last modified:January 23, 2007 - v2
Checksum:iAFCC258E574DE982
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17 – 18DK → KR AA sequence (PubMed:4463937).Curated2
Sequence conflicti29N → D AA sequence (PubMed:4463937).Curated1
Sequence conflicti145 – 146EQ → QE AA sequence (PubMed:4463937).Curated2
Sequence conflicti194S → T AA sequence (PubMed:4463937).Curated1
Sequence conflicti202 – 204QST → VQS AA sequence (PubMed:4463937).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11314 mRNA. Translation: AAA49094.1.
M11941 Genomic DNA. Translation: AAA49095.1.
PIRiA23448. ISCHT.
RefSeqiNP_990782.1. NM_205451.1.
UniGeneiGga.4148.

Genome annotation databases

EnsembliENSGALT00000023442; ENSGALP00000023396; ENSGALG00000014526.
ENSGALT00000071768; ENSGALP00000044536; ENSGALG00000014526.
GeneIDi396435.
KEGGigga:396435.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11314 mRNA. Translation: AAA49094.1.
M11941 Genomic DNA. Translation: AAA49095.1.
PIRiA23448. ISCHT.
RefSeqiNP_990782.1. NM_205451.1.
UniGeneiGga.4148.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SPQX-ray2.16A/B2-248[»]
1SQ7X-ray2.85A/B2-248[»]
1SSDX-ray2.90A/B2-248[»]
1SSGX-ray2.90A/B2-248[»]
1SU5X-ray2.70A/B2-248[»]
1SW0X-ray1.71A/B1-248[»]
1SW3X-ray2.03A/B1-248[»]
1SW7X-ray2.22A/B1-248[»]
1TIMX-ray2.50A/B2-248[»]
1TPBX-ray1.901/22-248[»]
1TPCX-ray1.901/22-248[»]
1TPHX-ray1.801/22-248[»]
1TPUX-ray1.90A/B2-248[»]
1TPVX-ray1.90A/B2-248[»]
1TPWX-ray1.90A/B2-248[»]
4P61X-ray1.34A/B1-248[»]
8TIMX-ray2.50A/B2-248[»]
ProteinModelPortaliP00940.
SMRiP00940.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi676684. 1 interactor.
IntActiP00940. 1 interactor.
STRINGi9031.ENSGALP00000023396.

Proteomic databases

PaxDbiP00940.
PRIDEiP00940.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000023442; ENSGALP00000023396; ENSGALG00000014526.
ENSGALT00000071768; ENSGALP00000044536; ENSGALG00000014526.
GeneIDi396435.
KEGGigga:396435.

Organism-specific databases

CTDi7167.

Phylogenomic databases

eggNOGiKOG1643. Eukaryota.
COG0149. LUCA.
GeneTreeiENSGT00390000013354.
HOGENOMiHOG000226413.
HOVERGENiHBG002599.
InParanoidiP00940.
KOiK01803.
OMAiIEKNGTM.
OrthoDBiEOG091G0LEZ.
PhylomeDBiP00940.
TreeFamiTF300829.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00189.
UPA00138.
BRENDAi5.3.1.1. 1306.
ReactomeiR-GGA-352875. Gluconeogenesis.
R-GGA-352882. Glycolysis.
R-GGA-70171. Glycolysis.
R-GGA-70263. Gluconeogenesis.
SABIO-RKP00940.

Miscellaneous databases

EvolutionaryTraceiP00940.
PROiP00940.

Gene expression databases

BgeeiENSGALG00000014526.

Family and domain databases

CDDicd00311. TIM. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPIS_CHICK
AccessioniPrimary (citable) accession number: P00940
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.