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Protein

Triosephosphate isomerase

Gene

TPI1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Triosephosphate isomerase (TPI1), Triosephosphate isomerase, Triosephosphate isomerase (TPI1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13Substrate1
Active sitei95Electrophile1
Active sitei165Proton acceptor1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processGluconeogenesis, Glycolysis

Enzyme and pathway databases

BRENDAi5.3.1.1 1306
ReactomeiR-GGA-352875 Gluconeogenesis
R-GGA-352882 Glycolysis
R-GGA-70171 Glycolysis
R-GGA-70263 Gluconeogenesis
SABIO-RKiP00940
UniPathwayiUPA00109; UER00189
UPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomerase (EC:5.3.1.1)
Short name:
TIM
Alternative name(s):
Triose-phosphate isomerase
Gene namesi
Name:TPI1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi95H → N: Reduces activity 5000-fold. 1 Publication1
Mutagenesisi165E → D: Reduces activity 300-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000901212 – 248Triosephosphate isomeraseAdd BLAST247

Proteomic databases

PaxDbiP00940
PRIDEiP00940

Expressioni

Gene expression databases

BgeeiENSGALG00000014526

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi676684, 1 interactor
IntActiP00940, 1 interactor
STRINGi9031.ENSGALP00000023396

Structurei

Secondary structure

1248
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 11Combined sources6
Helixi18 – 30Combined sources13
Beta strandi37 – 43Combined sources7
Helixi45 – 47Combined sources3
Helixi48 – 54Combined sources7
Beta strandi59 – 64Combined sources6
Beta strandi68 – 73Combined sources6
Helixi80 – 85Combined sources6
Beta strandi90 – 94Combined sources5
Helixi96 – 100Combined sources5
Helixi106 – 118Combined sources13
Beta strandi122 – 127Combined sources6
Helixi131 – 136Combined sources6
Helixi139 – 151Combined sources13
Helixi157 – 159Combined sources3
Beta strandi160 – 164Combined sources5
Helixi167 – 169Combined sources3
Beta strandi170 – 173Combined sources4
Helixi178 – 195Combined sources18
Helixi198 – 203Combined sources6
Beta strandi206 – 208Combined sources3
Turni214 – 216Combined sources3
Helixi217 – 221Combined sources5
Beta strandi228 – 232Combined sources5
Helixi233 – 236Combined sources4
Helixi240 – 244Combined sources5
Turni245 – 247Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SPQX-ray2.16A/B2-248[»]
1SQ7X-ray2.85A/B2-248[»]
1SSDX-ray2.90A/B2-248[»]
1SSGX-ray2.90A/B2-248[»]
1SU5X-ray2.70A/B2-248[»]
1SW0X-ray1.71A/B1-248[»]
1SW3X-ray2.03A/B1-248[»]
1SW7X-ray2.22A/B1-248[»]
1TIMX-ray2.50A/B2-248[»]
1TPBX-ray1.901/22-248[»]
1TPCX-ray1.901/22-248[»]
1TPHX-ray1.801/22-248[»]
1TPUX-ray1.90A/B2-248[»]
1TPVX-ray1.90A/B2-248[»]
1TPWX-ray1.90A/B2-248[»]
4P61X-ray1.34A/B1-248[»]
8TIMX-ray2.50A/B2-248[»]
ProteinModelPortaliP00940
SMRiP00940
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00940

Family & Domainsi

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.Curated

Phylogenomic databases

eggNOGiKOG1643 Eukaryota
COG0149 LUCA
GeneTreeiENSGT00390000013354
HOGENOMiHOG000226413
HOVERGENiHBG002599
InParanoidiP00940
KOiK01803
OMAiLCVGEGL
OrthoDBiEOG091G0LEZ
PhylomeDBiP00940
TreeFamiTF300829

Family and domain databases

CDDicd00311 TIM, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00147_B TIM_B, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR035990 TIM_sf
IPR022896 TrioseP_Isoase_bac/euk
IPR000652 Triosephosphate_isomerase
IPR020861 Triosephosphate_isomerase_AS
PANTHERiPTHR21139 PTHR21139, 1 hit
PfamiView protein in Pfam
PF00121 TIM, 1 hit
SUPFAMiSSF51351 SSF51351, 1 hit
TIGRFAMsiTIGR00419 tim, 1 hit
PROSITEiView protein in PROSITE
PS00171 TIM_1, 1 hit
PS51440 TIM_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00940-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPRKFFVGG NWKMNGDKKS LGELIHTLNG AKLSADTEVV CGAPSIYLDF
60 70 80 90 100
ARQKLDAKIG VAAQNCYKVP KGAFTGEISP AMIKDIGAAW VILGHSERRH
110 120 130 140 150
VFGESDELIG QKVAHALAEG LGVIACIGEK LDEREAGITE KVVFEQTKAI
160 170 180 190 200
ADNVKDWSKV VLAYEPVWAI GTGKTATPQQ AQEVHEKLRG WLKSHVSDAV
210 220 230 240
AQSTRIIYGG SVTGGNCKEL ASQHDVDGFL VGGASLKPEF VDIINAKH
Length:248
Mass (Da):26,620
Last modified:January 23, 2007 - v2
Checksum:iAFCC258E574DE982
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17 – 18DK → KR AA sequence (PubMed:4463937).Curated2
Sequence conflicti29N → D AA sequence (PubMed:4463937).Curated1
Sequence conflicti145 – 146EQ → QE AA sequence (PubMed:4463937).Curated2
Sequence conflicti194S → T AA sequence (PubMed:4463937).Curated1
Sequence conflicti202 – 204QST → VQS AA sequence (PubMed:4463937).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11314 mRNA Translation: AAA49094.1
M11941 Genomic DNA Translation: AAA49095.1
PIRiA23448 ISCHT
RefSeqiNP_990782.1, NM_205451.1
UniGeneiGga.4148

Genome annotation databases

EnsembliENSGALT00000023442; ENSGALP00000023396; ENSGALG00000014526
ENSGALT00000071768; ENSGALP00000044536; ENSGALG00000014526
GeneIDi396435
KEGGigga:396435

Similar proteinsi

Entry informationi

Entry nameiTPIS_CHICK
AccessioniPrimary (citable) accession number: P00940
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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