P00939 (TPIS_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triosephosphate isomerase
Short name=TIM
EC=5.3.1.1

Alternative name(s):
Triose-phosphate isomerase

Gene names

Name:

TPI1

Organism

Oryctolagus cuniculus (Rabbit) [Complete proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate.
Pathway	Carbohydrate biosynthesis; gluconeogenesis. Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.
Subunit structure	Homodimer. Ref.5
Post-translational modification	Asn-15 and Asn-71 undergo deamidation which gives rise to four extra negative charges. These are expected to decrease subunit-subunit interactions and so expose the hydrophobic interface to the aqueous environment.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Molecular function	Isomerase
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 248

248

Triosephosphate isomerase

PRO_0000090119

Sites

Active site

95

1

Electrophile By similarity

Active site

165

1

Proton acceptor By similarity Ref.4

Binding site

11

1

Substrate By similarity

Binding site

13

1

Substrate By similarity

Amino acid modifications

Modified residue

13

1

N6-acetyllysine By similarity

Modified residue

15

1

Deamidated asparagine Ref.4

Modified residue

71

1

Deamidated asparagine Ref.4

Modified residue

79

1

Phosphoserine By similarity

Modified residue

187

1

N6-acetyllysine By similarity

Modified residue

193

1

N6-acetyllysine By similarity

Modified residue

237

1

N6-acetyllysine By similarity

Secondary structure

1

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248

Helix Strand Turn

Details...

Beta strand

6 – 11

6

Helix

18 – 30

13

Beta strand

37 – 42

6

Helix

45 – 47

3

Helix

48 – 54

7

Beta strand

59 – 64

6

Beta strand

68 – 73

6

Helix

80 – 85

6

Beta strand

90 – 94

5

Helix

96 – 100

5

Helix

106 – 118

13

Beta strand

122 – 127

6

Helix

131 – 135

5

Helix

139 – 152

14

Helix

157 – 159

3

Beta strand

160 – 164

5

Helix

167 – 169

3

Beta strand

170 – 173

4

Helix

178 – 195

18

Helix

198 – 203

6

Beta strand

206 – 208

3

Turn

214 – 216

3

Helix

217 – 221

5

Beta strand

228 – 232

5

Helix

233 – 236

4

Helix

239 – 244

6

Sequences

	Sequence		Length	Mass (Da)
	P00939 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: E5ADDE9641370D81	FASTA	248	26,625
	10 20 30 40 50 60 APSRKFFVGG NWKMNGRKKN LGELITTLNA AKVPADTEVV CAPPTAYIDF ARQKLDPKIA 70 80 90 100 110 120 VAAQNCYKVT NGAFTGEISP GMIKDCGATW VVLGHSERRH VFGESDELIG QKVAHALSEG 130 140 150 160 170 180 LGVIACIGEK LDEREAGITE KVVFEQTKVI ADNVKDWSKV VLAYEPVWAI GTGKTATPQQ 190 200 210 220 230 240 AQEVHEKLRG WLKSNVSDAV AQSTRIIYGG SVTGATCKEL ASQPDVDGFL VGGASLKPEF VDIINAKQ

References

[1]	"The amino acid sequence of rabbit muscle triose phosphate isomerase." Corran P.H., Waley S.G. Biochem. J. 145:335-344(1975) [PubMed: 1171682] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"A new troponin T and cDNA clones for 13 different muscle proteins, found by shotgun sequencing." Putney S.D., Herlihy W.C., Schimmel P.R. Nature 302:718-721(1983) [PubMed: 6687628] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 56-83.
[3]	"Haloacetol phosphates. Characterization of the active site of rabbit muscle triose phosphate isomerase." Hartman F.C. Biochemistry 10:146-154(1971) [PubMed: 4922541] [Abstract] Cited for: ACTIVE SITE.
[4]	"Terminal marking of triosephosphate isomerase: consequences of deamidation." Sun A.-Q., Yueksel U., Gracy R.W. Arch. Biochem. Biophys. 322:361-368(1995) [PubMed: 7574709] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, DEAMIDATION AT ASN-15 AND ASN-71.
[5]	"Closed conformation of the active site loop of rabbit muscle triosephosphate isomerase in the absence of substrate: evidence of conformational heterogeneity." Aparicio R., Ferreira S.T., Polikarpov I. J. Mol. Biol. 334:1023-1041(2003) [PubMed: 14643664] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS), HOMODIMERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

V00902 mRNA. Translation: CAA24267.1.

PIR

ISRBT. A01165.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1R2R	X-ray	1.50	A/B/C/D	1-248	[»]
1R2S	X-ray	2.85	A/B/C/D	1-248	[»]
1R2T	X-ray	2.25	A/B	1-248	[»]

ProteinModelPortal

P00939.

SMR

P00939. Positions 2-248.

ModBase

Search...

Protein-protein interaction databases

STRING

P00939.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Phylogenomic databases

GeneTree

ENSGT00390000013354.

HOVERGEN

HBG002599.

OrthoDB

EOG40S0GF.

Family and domain databases

InterPro

IPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

PANTHER

PTHR21139. Triophos_ismrse. 1 hit.

Pfam

PF00121. TIM. 1 hit.
[Graphical view]

SUPFAM

SSF51351. Triophos_ismrse. 1 hit.

TIGRFAMs

TIGR00419. Tim. 1 hit.

PROSITE

PS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB01093. Dimethyl sulfoxide.

Entry information

Entry name

TPIS_RABIT

Accession

Primary (citable) accession number: P00939

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	November 16, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program