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Protein

Multifunctional tryptophan biosynthesis protein

Gene

TRP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1 and 4 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Multifunctional tryptophan biosynthesis protein (TRP3), Anthranilate synthase component 1 (TRP2)
  2. Anthranilate phosphoribosyltransferase (TRP4)
  3. N-(5'-phosphoribosyl)anthranilate isomerase (TRP1)
  4. Multifunctional tryptophan biosynthesis protein (TRP3)
  5. Tryptophan synthase (TRP5)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei92 – 921Nucleophile; for GATase activityPROSITE-ProRule annotation
Binding sitei96 – 961GlutamineBy similarity
Active sitei181 – 1811For GATase activityPROSITE-ProRule annotation
Active sitei183 – 1831For GATase activityPROSITE-ProRule annotation

GO - Molecular functioni

  • anthranilate synthase activity Source: UniProtKB-EC
  • indole-3-glycerol-phosphate synthase activity Source: SGD
  • transferase activity Source: UniProtKB-KW

GO - Biological processi

  • glutamine metabolic process Source: UniProtKB-KW
  • tryptophan biosynthetic process Source: SGD

Keywords - Molecular functioni

Lyase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YKL211C-MONOMER.
UniPathwayiUPA00035; UER00040.
UPA00035; UER00043.

Protein family/group databases

MEROPSiC26.959.

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional tryptophan biosynthesis protein
Including the following 2 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Alternative name(s):
PRAI
Gene namesi
Name:TRP3
Ordered Locus Names:YKL211C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL211C.
SGDiS000001694. TRP3.

Subcellular locationi

GO - Cellular componenti

  • anthranilate synthase complex Source: SGD
  • cytosol Source: GO_Central

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484Multifunctional tryptophan biosynthesis proteinPRO_0000056866Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP00937.

PTM databases

iPTMnetiP00937.

Expressioni

Inductioni

By tryptophan starvation.

Interactioni

Subunit structurei

Tetramer of two components I and two components II.

Binary interactionsi

WithEntry#Exp.IntActNotes
TRP2P008994EBI-19585,EBI-19575

Protein-protein interaction databases

BioGridi33954. 61 interactions.
DIPiDIP-543N.
IntActiP00937. 32 interactions.
MINTiMINT-616948.

Structurei

3D structure databases

ProteinModelPortaliP00937.
SMRiP00937. Positions 14-198, 215-482.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 207195Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni64 – 663Glutamine bindingBy similarity
Regioni142 – 1432Glutamine bindingBy similarity
Regioni215 – 484270Indole-3-glycerol phosphate synthaseAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

GeneTreeiENSGT00620000088738.
HOGENOMiHOG000280459.
InParanoidiP00937.
KOiK01656.
OMAiVAVTRYH.
OrthoDBiEOG092C1PKC.

Family and domain databases

CDDicd00331. IGPS. 1 hit.
Gene3Di3.20.20.70. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00937-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVHAATNPI NKHVVLIDNY DSFTWNVYEY LCQEGAKVSV YRNDAITVPE
60 70 80 90 100
IAALNPDTLL ISPGPGHPKT DSGISRDCIR YFTGKIPVFG ICMGQQCMFD
110 120 130 140 150
VFGGEVAYAG EIVHGKTSPI SHDNCGIFKN VPQGIAVTRY HSLAGTESSL
160 170 180 190 200
PSCLKVTAST ENGIIMGVRH KKYTVEGVQF HPESILTEEG HLMIRNILNV
210 220 230 240 250
SGGTWEENKS SPSNSILDRI YARRKIDVNE QSKIPGFTFQ DLQSNYDLGL
260 270 280 290 300
APPLQDFYTV LSSSHKRAVV LAEVKRASPS KGPICLKAVA AEQALKYAEA
310 320 330 340 350
GASAISVLTE PHWFHGSLQD LVNVRKILDL KFPPKERPCV LRKEFIFSKY
360 370 380 390 400
QILEARLAGA DTVLLIVKML SQPLLKELYS YSKDLNMEPL VEVNSKEELQ
410 420 430 440 450
RALEIGAKVV GVNNRDLHSF NVDLNTTSNL VESIPKDVLL IALSGITTRD
460 470 480
DAEKYKKEGV HGFLVGEALM KSTDVKKFIH ELCE
Length:484
Mass (Da):53,489
Last modified:June 1, 1994 - v2
Checksum:i34EF65E829279C1F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321C → S in AAA34450 (Ref. 5) Curated
Sequence conflicti63 – 653PGP → LGL in AAA34450 (Ref. 5) Curated
Sequence conflicti129 – 1291K → R in AAA35176 (PubMed:6323449).Curated
Sequence conflicti170 – 1701H → Y in AAA34450 (Ref. 5) Curated
Sequence conflicti236 – 2361G → S in AAA34450 (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01386 Genomic DNA. Translation: AAA35176.1.
X75951 Genomic DNA. Translation: CAA53562.1.
Z28211 Genomic DNA. Translation: CAA82056.1.
M36300 Genomic DNA. Translation: AAA34450.1.
BK006944 Genomic DNA. Translation: DAA08958.1.
PIRiS38049. NNBY2.
RefSeqiNP_012711.1. NM_001179776.1.

Genome annotation databases

EnsemblFungiiYKL211C; YKL211C; YKL211C.
GeneIDi853669.
KEGGisce:YKL211C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01386 Genomic DNA. Translation: AAA35176.1.
X75951 Genomic DNA. Translation: CAA53562.1.
Z28211 Genomic DNA. Translation: CAA82056.1.
M36300 Genomic DNA. Translation: AAA34450.1.
BK006944 Genomic DNA. Translation: DAA08958.1.
PIRiS38049. NNBY2.
RefSeqiNP_012711.1. NM_001179776.1.

3D structure databases

ProteinModelPortaliP00937.
SMRiP00937. Positions 14-198, 215-482.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33954. 61 interactions.
DIPiDIP-543N.
IntActiP00937. 32 interactions.
MINTiMINT-616948.

Protein family/group databases

MEROPSiC26.959.

PTM databases

iPTMnetiP00937.

Proteomic databases

MaxQBiP00937.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL211C; YKL211C; YKL211C.
GeneIDi853669.
KEGGisce:YKL211C.

Organism-specific databases

EuPathDBiFungiDB:YKL211C.
SGDiS000001694. TRP3.

Phylogenomic databases

GeneTreeiENSGT00620000088738.
HOGENOMiHOG000280459.
InParanoidiP00937.
KOiK01656.
OMAiVAVTRYH.
OrthoDBiEOG092C1PKC.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
UPA00035; UER00043.
BioCyciYEAST:YKL211C-MONOMER.

Miscellaneous databases

PROiP00937.

Family and domain databases

CDDicd00331. IGPS. 1 hit.
Gene3Di3.20.20.70. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPG_YEAST
AccessioniPrimary (citable) accession number: P00937
Secondary accession number(s): D6VWZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 167 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Yeast component II C-terminal half also has indole-3-glycerol phosphate synthase activity.
Present with 13400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.