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Protein

Multifunctional tryptophan biosynthesis protein

Gene

TRP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Yeast component II C-terminal half also has indole-3-glycerol phosphate synthase activity.
Present with 13400 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1 and 4 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Multifunctional tryptophan biosynthesis protein (TRP3), Anthranilate synthase component 1 (TRP2)
  2. Anthranilate phosphoribosyltransferase (TRP4)
  3. N-(5'-phosphoribosyl)anthranilate isomerase (TRP1)
  4. Multifunctional tryptophan biosynthesis protein (TRP3)
  5. Tryptophan synthase (TRP5)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei92Nucleophile; for GATase activityPROSITE-ProRule annotation1
Binding sitei96GlutamineBy similarity1
Active sitei181For GATase activityPROSITE-ProRule annotation1
Active sitei183For GATase activityPROSITE-ProRule annotation1

GO - Molecular functioni

  • anthranilate synthase activity Source: UniProtKB-EC
  • indole-3-glycerol-phosphate synthase activity Source: SGD

GO - Biological processi

  • glutamine metabolic process Source: UniProtKB-KW
  • tryptophan biosynthetic process Source: SGD

Keywordsi

Molecular functionLyase, Multifunctional enzyme
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YKL211C-MONOMER
UniPathwayiUPA00035; UER00040
UPA00035; UER00043

Protein family/group databases

MEROPSiC26.959

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional tryptophan biosynthesis protein
Including the following 2 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Alternative name(s):
PRAI
Gene namesi
Name:TRP3
Ordered Locus Names:YKL211C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL211C
SGDiS000001694 TRP3

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000568661 – 484Multifunctional tryptophan biosynthesis proteinAdd BLAST484

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP00937
PaxDbiP00937
PRIDEiP00937

PTM databases

iPTMnetiP00937

Expressioni

Inductioni

By tryptophan starvation.

Interactioni

Subunit structurei

Tetramer of two components I and two components II.

Binary interactionsi

WithEntry#Exp.IntActNotes
TRP2P008993EBI-19585,EBI-19575

Protein-protein interaction databases

BioGridi33954, 89 interactors
DIPiDIP-543N
IntActiP00937, 36 interactors
MINTiP00937
STRINGi4932.YKL211C

Structurei

3D structure databases

ProteinModelPortaliP00937
SMRiP00937
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 207Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd BLAST195

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni64 – 66Glutamine bindingBy similarity3
Regioni142 – 143Glutamine bindingBy similarity2
Regioni215 – 484Indole-3-glycerol phosphate synthaseAdd BLAST270

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

GeneTreeiENSGT00620000088738
HOGENOMiHOG000280459
InParanoidiP00937
KOiK01656
OMAiWFKGSIE
OrthoDBiEOG092C1PKC

Family and domain databases

CDDicd00331 IGPS, 1 hit
Gene3Di3.20.20.70, 1 hit
3.40.50.880, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR013798 Indole-3-glycerol_P_synth
IPR001468 Indole-3-GlycerolPSynthase_CS
IPR011060 RibuloseP-bd_barrel
IPR006221 TrpG/PapA_dom
PfamiView protein in Pfam
PF00117 GATase, 1 hit
PF00218 IGPS, 1 hit
SUPFAMiSSF51366 SSF51366, 1 hit
SSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR00566 trpG_papA, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit
PS00614 IGPS, 1 hit

Sequencei

Sequence statusi: Complete.

P00937-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVHAATNPI NKHVVLIDNY DSFTWNVYEY LCQEGAKVSV YRNDAITVPE
60 70 80 90 100
IAALNPDTLL ISPGPGHPKT DSGISRDCIR YFTGKIPVFG ICMGQQCMFD
110 120 130 140 150
VFGGEVAYAG EIVHGKTSPI SHDNCGIFKN VPQGIAVTRY HSLAGTESSL
160 170 180 190 200
PSCLKVTAST ENGIIMGVRH KKYTVEGVQF HPESILTEEG HLMIRNILNV
210 220 230 240 250
SGGTWEENKS SPSNSILDRI YARRKIDVNE QSKIPGFTFQ DLQSNYDLGL
260 270 280 290 300
APPLQDFYTV LSSSHKRAVV LAEVKRASPS KGPICLKAVA AEQALKYAEA
310 320 330 340 350
GASAISVLTE PHWFHGSLQD LVNVRKILDL KFPPKERPCV LRKEFIFSKY
360 370 380 390 400
QILEARLAGA DTVLLIVKML SQPLLKELYS YSKDLNMEPL VEVNSKEELQ
410 420 430 440 450
RALEIGAKVV GVNNRDLHSF NVDLNTTSNL VESIPKDVLL IALSGITTRD
460 470 480
DAEKYKKEGV HGFLVGEALM KSTDVKKFIH ELCE
Length:484
Mass (Da):53,489
Last modified:June 1, 1994 - v2
Checksum:i34EF65E829279C1F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32C → S in AAA34450 (Ref. 5) Curated1
Sequence conflicti63 – 65PGP → LGL in AAA34450 (Ref. 5) Curated3
Sequence conflicti129K → R in AAA35176 (PubMed:6323449).Curated1
Sequence conflicti170H → Y in AAA34450 (Ref. 5) Curated1
Sequence conflicti236G → S in AAA34450 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01386 Genomic DNA Translation: AAA35176.1
X75951 Genomic DNA Translation: CAA53562.1
Z28211 Genomic DNA Translation: CAA82056.1
M36300 Genomic DNA Translation: AAA34450.1
BK006944 Genomic DNA Translation: DAA08958.1
PIRiS38049 NNBY2
RefSeqiNP_012711.1, NM_001179776.1

Genome annotation databases

EnsemblFungiiYKL211C; YKL211C; YKL211C
GeneIDi853669
KEGGisce:YKL211C

Similar proteinsi

Entry informationi

Entry nameiTRPG_YEAST
AccessioniPrimary (citable) accession number: P00937
Secondary accession number(s): D6VWZ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 181 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names
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Main funding by: National Institutes of Health