Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P00936

- CYAA_ECOLI

UniProt

P00936 - CYAA_ECOLI

Protein

Adenylate cyclase

Gene

cyaA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 5 (20 Jun 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the formation of the second messenger cAMP from ATP. Its transcript is probably degraded by endoribonuclease LS (rnlA), decreasing cAMP levels and the negative regulator Crp-cAMP, which then induces its own transcription again.

    Catalytic activityi

    ATP = 3',5'-cyclic AMP + diphosphate.

    Enzyme regulationi

    The regulatory domain is involved in the regulation of cyclase activity by the carbon source. Activated by the PTS system, glucose-specific IIA component (CRR).

    GO - Molecular functioni

    1. adenylate cyclase activity Source: EcoCyc
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. cAMP biosynthetic process Source: EcoCyc

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    cAMP biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:ADENYLATECYC-MONOMER.
    ECOL316407:JW3778-MONOMER.
    MetaCyc:ADENYLATECYC-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenylate cyclase (EC:4.6.1.1)
    Alternative name(s):
    ATP pyrophosphate-lyase
    Adenylyl cyclase
    Gene namesi
    Name:cyaA
    Synonyms:cya
    Ordered Locus Names:b3806, JW3778
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10170. cyaA.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Not essential, eliminates the NaCl sensitivity of an rnlA deletion mutant.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 848848Adenylate cyclasePRO_0000195669Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei609 – 6091Phosphohistidine; by CRRSequence Analysis

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP00936.
    PRIDEiP00936.

    PTM databases

    PhosSiteiP0809376.

    Expressioni

    Inductioni

    Repressed by the Crp-cAMP complex. Expression increases during growth, decreasing again in stationary phase; more strongly induced in an rnlA deletion mutant, levels remain high even in stationary phase (at protein level).2 Publications

    Gene expression databases

    GenevestigatoriP00936.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    helDP150383EBI-1116685,EBI-551473

    Protein-protein interaction databases

    IntActiP00936. 23 interactions.
    STRINGi511145.b3806.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 535535CatalyticAdd
    BLAST
    Regioni541 – 848308RegulatoryAdd
    BLAST

    Sequence similaritiesi

    Belongs to the adenylyl cyclase class-1 family.Curated

    Phylogenomic databases

    eggNOGiCOG3072.
    HOGENOMiHOG000270910.
    KOiK05851.
    OMAiSEGFLQF.
    OrthoDBiEOG63JR9H.

    Family and domain databases

    InterProiIPR000274. Adenylate_cyclase_1.
    IPR024686. Adenylate_cyclase_1_CS.
    IPR024685. Adenylate_cyclase_1_N.
    [Graphical view]
    PfamiPF12633. Adenyl_cycl_N. 1 hit.
    PF01295. Adenylate_cycl. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001444. Adenylate_cycl. 1 hit.
    PROSITEiPS01092. ADENYLATE_CYCLASE_1_1. 1 hit.
    PS01093. ADENYLATE_CYCLASE_1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00936-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYLYIETLKQ RLDAINQLRV DRALAAMGPA FQQVYSLLPT LLHYHHPLMP    50
    GYLDGNVPKG ICLYTPDETQ RHYLNELELY RGMSVQDPPK GELPITGVYT 100
    MGSTSSVGQS CSSDLDIWVC HQSWLDSEER QLLQRKCSLL ENWAASLGVE 150
    VSFFLIDENR FRHNESGSLG GEDCGSTQHI LLLDEFYRTA VRLAGKRILW 200
    NMVPCDEEEH YDDYVMTLYA QGVLTPNEWL DLGGLSSLSA EEYFGASLWQ 250
    LYKSIDSPYK AVLKTLLLEA YSWEYPNPRL LAKDIKQRLH DGEIVSFGLD 300
    PYCMMLERVT EYLTAIEDFT RLDLVRRCFY LKVCEKLSRE RACVGWRRAV 350
    LSQLVSEWGW DEARLAMLDN RANWKIDQVR EAHNELLDAM MQSYRNLIRF 400
    ARRNNLSVSA SPQDIGVLTR KLYAAFEALP GKVTLVNPQI SPDLSEPNLT 450
    FIYVPPGRAN RSGWYLYNRA PNIESIISHQ PLEYNRYLNK LVAWAWFNGL 500
    LTSRTRLYIK GNGIVDLPKL QEMVADVSHH FPLRLPAPTP KALYSPCEIR 550
    HLAIIVNLEY DPTAAFRNQV VHFDFRKLDV FSFGENQNCL VGSVDLLYRN 600
    SWNEVRTLHF NGEQSMIEAL KTILGKMHQD AAPPDSVEVF CYSQHLRGLI 650
    RTRVQQLVSE CIELRLSSTR QETGRFKALR VSGQTWGLFF ERLNVSVQKL 700
    ENAIEFYGAI SHNKLHGLSV QVETNHVKLP AVVDGFASEG IIQFFFEETQ 750
    DENGFNIYIL DESNRVEVYH HCEGSKEELV RDVSRFYSSS HDRFTYGSSF 800
    INFNLPQFYQ IVKVDGREQV IPFRTKSIGN MPPANQDHDT PLLQQYFS 848
    Length:848
    Mass (Da):97,586
    Last modified:June 20, 2003 - v5
    Checksum:iF0400E3406B15018
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti223 – 2231V → G in CAA25817. (PubMed:6393056)Curated
    Sequence conflicti223 – 2231V → G in CAA47280. (PubMed:8874804)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01653 Genomic DNA. Translation: CAA25817.1.
    X66782 Genomic DNA. Translation: CAA47280.1.
    M87049 Genomic DNA. Translation: AAA67602.1.
    U00096 Genomic DNA. Translation: AAC76809.1.
    AP009048 Genomic DNA. Translation: BAE77495.1.
    PIRiG65184. OYEC.
    RefSeqiNP_418250.1. NC_000913.3.
    YP_491636.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76809; AAC76809; b3806.
    BAE77495; BAE77495; BAE77495.
    GeneIDi12933616.
    947755.
    KEGGiecj:Y75_p3372.
    eco:b3806.
    PATRICi32123111. VBIEscCol129921_3921.

    Cross-referencesi

    Web resourcesi

    Escherichia coli adenylate cyclase homepage

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01653 Genomic DNA. Translation: CAA25817.1 .
    X66782 Genomic DNA. Translation: CAA47280.1 .
    M87049 Genomic DNA. Translation: AAA67602.1 .
    U00096 Genomic DNA. Translation: AAC76809.1 .
    AP009048 Genomic DNA. Translation: BAE77495.1 .
    PIRi G65184. OYEC.
    RefSeqi NP_418250.1. NC_000913.3.
    YP_491636.1. NC_007779.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P00936. 23 interactions.
    STRINGi 511145.b3806.

    PTM databases

    PhosSitei P0809376.

    Proteomic databases

    PaxDbi P00936.
    PRIDEi P00936.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76809 ; AAC76809 ; b3806 .
    BAE77495 ; BAE77495 ; BAE77495 .
    GeneIDi 12933616.
    947755.
    KEGGi ecj:Y75_p3372.
    eco:b3806.
    PATRICi 32123111. VBIEscCol129921_3921.

    Organism-specific databases

    EchoBASEi EB0168.
    EcoGenei EG10170. cyaA.

    Phylogenomic databases

    eggNOGi COG3072.
    HOGENOMi HOG000270910.
    KOi K05851.
    OMAi SEGFLQF.
    OrthoDBi EOG63JR9H.

    Enzyme and pathway databases

    BioCyci EcoCyc:ADENYLATECYC-MONOMER.
    ECOL316407:JW3778-MONOMER.
    MetaCyc:ADENYLATECYC-MONOMER.

    Miscellaneous databases

    PROi P00936.

    Gene expression databases

    Genevestigatori P00936.

    Family and domain databases

    InterProi IPR000274. Adenylate_cyclase_1.
    IPR024686. Adenylate_cyclase_1_CS.
    IPR024685. Adenylate_cyclase_1_N.
    [Graphical view ]
    Pfami PF12633. Adenyl_cycl_N. 1 hit.
    PF01295. Adenylate_cycl. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001444. Adenylate_cycl. 1 hit.
    PROSITEi PS01092. ADENYLATE_CYCLASE_1_1. 1 hit.
    PS01093. ADENYLATE_CYCLASE_1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete nucleotide sequence of the adenylate cyclase gene of Escherichia coli."
      Aiba H., Mori K., Tanaka M., Ooi T., Roy A., Danchin A.
      Nucleic Acids Res. 12:9427-9440(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Transcription of the Escherichia coli adenylate cyclase gene is negatively regulated by cAMP-cAMP receptor protein."
      Aiba H.
      J. Biol. Chem. 260:3063-3070(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    3. "Comparative analysis of the cya locus in enterobacteria and related Gram-negative facultative anaerobes."
      Trotot P., Sismeiro O., Vivares C., Glaser P., Bresson-Roy A., Danchin A.
      Biochimie 78:277-287(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
      Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
      Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    7. "Regulation of adenylate cyclase synthesis in Escherichia coli: nucleotide sequence of the control region."
      Roy A., Haziza C., Danchin A.
      EMBO J. 2:791-797(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-183.
    8. "Cloning and promoter analysis of the Escherichia coli adenylate cyclase gene."
      Aiba H., Kawamukai M., Ishihama A.
      Nucleic Acids Res. 11:3451-3465(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 27-88.
    9. "Post-transcriptional control of Crp-cAMP by RNase LS in Escherichia coli."
      Iwamoto A., Lemire S., Yonesaki T.
      Mol. Microbiol. 70:1570-1578(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, REGULATION, DISRUPTION PHENOTYPE.
      Strain: K12.
    10. "Phylogeny of adenylyl cyclases."
      Danchin A.
      Adv. Second Messenger Phosphoprotein Res. 27:109-162(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiCYAA_ECOLI
    AccessioniPrimary (citable) accession number: P00936
    Secondary accession number(s): Q2M8B1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: June 20, 2003
    Last modified: October 1, 2014
    This is version 123 of the entry and version 5 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3