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Protein

Adenylate cyclase

Gene

cyaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the second messenger cAMP from ATP. Its transcript is probably degraded by endoribonuclease LS (rnlA), decreasing cAMP levels and the negative regulator Crp-cAMP, which then induces its own transcription again.

Catalytic activityi

ATP = 3',5'-cyclic AMP + diphosphate.

Enzyme regulationi

The regulatory domain is involved in the regulation of cyclase activity by the carbon source. Activated by the PTS system, glucose-specific IIA component (CRR).

GO - Molecular functioni

  • adenylate cyclase activity Source: EcoCyc
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • cAMP biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

cAMP biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ADENYLATECYC-MONOMER.
ECOL316407:JW3778-MONOMER.
MetaCyc:ADENYLATECYC-MONOMER.
BRENDAi4.6.1.1. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate cyclase (EC:4.6.1.1)
Alternative name(s):
ATP pyrophosphate-lyase
Adenylyl cyclase
Gene namesi
Name:cyaA
Synonyms:cya
Ordered Locus Names:b3806, JW3778
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10170. cyaA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Not essential, eliminates the NaCl sensitivity of an rnlA deletion mutant.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 848848Adenylate cyclasePRO_0000195669Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei609 – 6091Phosphohistidine; by CRRSequence Analysis

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP00936.
PRIDEiP00936.

Expressioni

Inductioni

Repressed by the Crp-cAMP complex. Expression increases during growth, decreasing again in stationary phase; more strongly induced in an rnlA deletion mutant, levels remain high even in stationary phase (at protein level).2 Publications

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
helDP150383EBI-1116685,EBI-551473

Protein-protein interaction databases

IntActiP00936. 23 interactions.
STRINGi511145.b3806.

Structurei

3D structure databases

ProteinModelPortaliP00936.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 535535CatalyticAdd
BLAST
Regioni541 – 848308RegulatoryAdd
BLAST

Sequence similaritiesi

Belongs to the adenylyl cyclase class-1 family.Curated

Phylogenomic databases

eggNOGiCOG3072.
HOGENOMiHOG000270910.
InParanoidiP00936.
KOiK05851.
OMAiPIHGLFL.
OrthoDBiEOG63JR9H.

Family and domain databases

InterProiIPR000274. Adenylate_cyclase_1.
IPR024686. Adenylate_cyclase_1_CS.
IPR024685. Adenylate_cyclase_1_N.
[Graphical view]
PfamiPF12633. Adenyl_cycl_N. 1 hit.
PF01295. Adenylate_cycl. 1 hit.
[Graphical view]
PIRSFiPIRSF001444. Adenylate_cycl. 1 hit.
PROSITEiPS01092. ADENYLATE_CYCLASE_1_1. 1 hit.
PS01093. ADENYLATE_CYCLASE_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00936-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYLYIETLKQ RLDAINQLRV DRALAAMGPA FQQVYSLLPT LLHYHHPLMP
60 70 80 90 100
GYLDGNVPKG ICLYTPDETQ RHYLNELELY RGMSVQDPPK GELPITGVYT
110 120 130 140 150
MGSTSSVGQS CSSDLDIWVC HQSWLDSEER QLLQRKCSLL ENWAASLGVE
160 170 180 190 200
VSFFLIDENR FRHNESGSLG GEDCGSTQHI LLLDEFYRTA VRLAGKRILW
210 220 230 240 250
NMVPCDEEEH YDDYVMTLYA QGVLTPNEWL DLGGLSSLSA EEYFGASLWQ
260 270 280 290 300
LYKSIDSPYK AVLKTLLLEA YSWEYPNPRL LAKDIKQRLH DGEIVSFGLD
310 320 330 340 350
PYCMMLERVT EYLTAIEDFT RLDLVRRCFY LKVCEKLSRE RACVGWRRAV
360 370 380 390 400
LSQLVSEWGW DEARLAMLDN RANWKIDQVR EAHNELLDAM MQSYRNLIRF
410 420 430 440 450
ARRNNLSVSA SPQDIGVLTR KLYAAFEALP GKVTLVNPQI SPDLSEPNLT
460 470 480 490 500
FIYVPPGRAN RSGWYLYNRA PNIESIISHQ PLEYNRYLNK LVAWAWFNGL
510 520 530 540 550
LTSRTRLYIK GNGIVDLPKL QEMVADVSHH FPLRLPAPTP KALYSPCEIR
560 570 580 590 600
HLAIIVNLEY DPTAAFRNQV VHFDFRKLDV FSFGENQNCL VGSVDLLYRN
610 620 630 640 650
SWNEVRTLHF NGEQSMIEAL KTILGKMHQD AAPPDSVEVF CYSQHLRGLI
660 670 680 690 700
RTRVQQLVSE CIELRLSSTR QETGRFKALR VSGQTWGLFF ERLNVSVQKL
710 720 730 740 750
ENAIEFYGAI SHNKLHGLSV QVETNHVKLP AVVDGFASEG IIQFFFEETQ
760 770 780 790 800
DENGFNIYIL DESNRVEVYH HCEGSKEELV RDVSRFYSSS HDRFTYGSSF
810 820 830 840
INFNLPQFYQ IVKVDGREQV IPFRTKSIGN MPPANQDHDT PLLQQYFS
Length:848
Mass (Da):97,586
Last modified:June 20, 2003 - v5
Checksum:iF0400E3406B15018
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti223 – 2231V → G in CAA25817 (PubMed:6393056).Curated
Sequence conflicti223 – 2231V → G in CAA47280 (PubMed:8874804).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01653 Genomic DNA. Translation: CAA25817.1.
X66782 Genomic DNA. Translation: CAA47280.1.
M87049 Genomic DNA. Translation: AAA67602.1.
U00096 Genomic DNA. Translation: AAC76809.1.
AP009048 Genomic DNA. Translation: BAE77495.1.
PIRiG65184. OYEC.
RefSeqiNP_418250.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC76809; AAC76809; b3806.
BAE77495; BAE77495; BAE77495.
GeneIDi947755.
KEGGiecj:Y75_p3372.
eco:b3806.
PATRICi32123111. VBIEscCol129921_3921.

Cross-referencesi

Web resourcesi

Escherichia coli adenylate cyclase homepage

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01653 Genomic DNA. Translation: CAA25817.1.
X66782 Genomic DNA. Translation: CAA47280.1.
M87049 Genomic DNA. Translation: AAA67602.1.
U00096 Genomic DNA. Translation: AAC76809.1.
AP009048 Genomic DNA. Translation: BAE77495.1.
PIRiG65184. OYEC.
RefSeqiNP_418250.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP00936.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP00936. 23 interactions.
STRINGi511145.b3806.

Proteomic databases

PaxDbiP00936.
PRIDEiP00936.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76809; AAC76809; b3806.
BAE77495; BAE77495; BAE77495.
GeneIDi947755.
KEGGiecj:Y75_p3372.
eco:b3806.
PATRICi32123111. VBIEscCol129921_3921.

Organism-specific databases

EchoBASEiEB0168.
EcoGeneiEG10170. cyaA.

Phylogenomic databases

eggNOGiCOG3072.
HOGENOMiHOG000270910.
InParanoidiP00936.
KOiK05851.
OMAiPIHGLFL.
OrthoDBiEOG63JR9H.

Enzyme and pathway databases

BioCyciEcoCyc:ADENYLATECYC-MONOMER.
ECOL316407:JW3778-MONOMER.
MetaCyc:ADENYLATECYC-MONOMER.
BRENDAi4.6.1.1. 2026.

Miscellaneous databases

PROiP00936.

Family and domain databases

InterProiIPR000274. Adenylate_cyclase_1.
IPR024686. Adenylate_cyclase_1_CS.
IPR024685. Adenylate_cyclase_1_N.
[Graphical view]
PfamiPF12633. Adenyl_cycl_N. 1 hit.
PF01295. Adenylate_cycl. 1 hit.
[Graphical view]
PIRSFiPIRSF001444. Adenylate_cycl. 1 hit.
PROSITEiPS01092. ADENYLATE_CYCLASE_1_1. 1 hit.
PS01093. ADENYLATE_CYCLASE_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete nucleotide sequence of the adenylate cyclase gene of Escherichia coli."
    Aiba H., Mori K., Tanaka M., Ooi T., Roy A., Danchin A.
    Nucleic Acids Res. 12:9427-9440(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Transcription of the Escherichia coli adenylate cyclase gene is negatively regulated by cAMP-cAMP receptor protein."
    Aiba H.
    J. Biol. Chem. 260:3063-3070(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
  3. "Comparative analysis of the cya locus in enterobacteria and related Gram-negative facultative anaerobes."
    Trotot P., Sismeiro O., Vivares C., Glaser P., Bresson-Roy A., Danchin A.
    Biochimie 78:277-287(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  7. "Regulation of adenylate cyclase synthesis in Escherichia coli: nucleotide sequence of the control region."
    Roy A., Haziza C., Danchin A.
    EMBO J. 2:791-797(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-183.
  8. "Cloning and promoter analysis of the Escherichia coli adenylate cyclase gene."
    Aiba H., Kawamukai M., Ishihama A.
    Nucleic Acids Res. 11:3451-3465(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 27-88.
  9. "Post-transcriptional control of Crp-cAMP by RNase LS in Escherichia coli."
    Iwamoto A., Lemire S., Yonesaki T.
    Mol. Microbiol. 70:1570-1578(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, REGULATION, DISRUPTION PHENOTYPE.
    Strain: K12.
  10. "Phylogeny of adenylyl cyclases."
    Danchin A.
    Adv. Second Messenger Phosphoprotein Res. 27:109-162(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiCYAA_ECOLI
AccessioniPrimary (citable) accession number: P00936
Secondary accession number(s): Q2M8B1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 20, 2003
Last modified: June 24, 2015
This is version 129 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.