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P00936 (CYAA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenylate cyclase

EC=4.6.1.1
Alternative name(s):
ATP pyrophosphate-lyase
Adenylyl cyclase
Gene names
Name:cyaA
Synonyms:cya
Ordered Locus Names:b3806, JW3778
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length848 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the formation of the second messenger cAMP from ATP. Its transcript is probably degraded by endoribonuclease LS (rnlA), decreasing cAMP levels and the negative regulator Crp-cAMP, which then induces its own transcription again.

Catalytic activity

ATP = 3',5'-cyclic AMP + diphosphate.

Enzyme regulation

The regulatory domain is involved in the regulation of cyclase activity by the carbon source. Activated by the PTS system, glucose-specific IIA component (CRR).

Subcellular location

Cytoplasm.

Induction

Repressed by the Crp-cAMP complex. Expression increases during growth, decreasing again in stationary phase; more strongly induced in an rnlA deletion mutant, levels remain high even in stationary phase (at protein level). Ref.2 Ref.9

Disruption phenotype

Not essential, eliminates the NaCl sensitivity of an rnlA deletion mutant. Ref.9

Sequence similarities

Belongs to the adenylyl cyclase class-1 family.

Ontologies

Keywords
   Biological processcAMP biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLyase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcAMP biosynthetic process

Inferred from mutant phenotype PubMed 4310317. Source: EcoCyc

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

adenylate cyclase activity

Inferred from direct assay PubMed 6300054. Source: EcoCyc

protein binding

Inferred from physical interaction PubMed 24561554. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

helDP150383EBI-1116685,EBI-551473

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 848848Adenylate cyclase
PRO_0000195669

Regions

Region1 – 535535Catalytic
Region541 – 848308Regulatory

Amino acid modifications

Modified residue6091Phosphohistidine; by CRR Potential

Experimental info

Sequence conflict2231V → G in CAA25817. Ref.1
Sequence conflict2231V → G in CAA47280. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P00936 [UniParc].

Last modified June 20, 2003. Version 5.
Checksum: F0400E3406B15018

FASTA84897,586
        10         20         30         40         50         60 
MYLYIETLKQ RLDAINQLRV DRALAAMGPA FQQVYSLLPT LLHYHHPLMP GYLDGNVPKG 

        70         80         90        100        110        120 
ICLYTPDETQ RHYLNELELY RGMSVQDPPK GELPITGVYT MGSTSSVGQS CSSDLDIWVC 

       130        140        150        160        170        180 
HQSWLDSEER QLLQRKCSLL ENWAASLGVE VSFFLIDENR FRHNESGSLG GEDCGSTQHI 

       190        200        210        220        230        240 
LLLDEFYRTA VRLAGKRILW NMVPCDEEEH YDDYVMTLYA QGVLTPNEWL DLGGLSSLSA 

       250        260        270        280        290        300 
EEYFGASLWQ LYKSIDSPYK AVLKTLLLEA YSWEYPNPRL LAKDIKQRLH DGEIVSFGLD 

       310        320        330        340        350        360 
PYCMMLERVT EYLTAIEDFT RLDLVRRCFY LKVCEKLSRE RACVGWRRAV LSQLVSEWGW 

       370        380        390        400        410        420 
DEARLAMLDN RANWKIDQVR EAHNELLDAM MQSYRNLIRF ARRNNLSVSA SPQDIGVLTR 

       430        440        450        460        470        480 
KLYAAFEALP GKVTLVNPQI SPDLSEPNLT FIYVPPGRAN RSGWYLYNRA PNIESIISHQ 

       490        500        510        520        530        540 
PLEYNRYLNK LVAWAWFNGL LTSRTRLYIK GNGIVDLPKL QEMVADVSHH FPLRLPAPTP 

       550        560        570        580        590        600 
KALYSPCEIR HLAIIVNLEY DPTAAFRNQV VHFDFRKLDV FSFGENQNCL VGSVDLLYRN 

       610        620        630        640        650        660 
SWNEVRTLHF NGEQSMIEAL KTILGKMHQD AAPPDSVEVF CYSQHLRGLI RTRVQQLVSE 

       670        680        690        700        710        720 
CIELRLSSTR QETGRFKALR VSGQTWGLFF ERLNVSVQKL ENAIEFYGAI SHNKLHGLSV 

       730        740        750        760        770        780 
QVETNHVKLP AVVDGFASEG IIQFFFEETQ DENGFNIYIL DESNRVEVYH HCEGSKEELV 

       790        800        810        820        830        840 
RDVSRFYSSS HDRFTYGSSF INFNLPQFYQ IVKVDGREQV IPFRTKSIGN MPPANQDHDT 


PLLQQYFS 

« Hide

References

« Hide 'large scale' references
[1]"The complete nucleotide sequence of the adenylate cyclase gene of Escherichia coli."
Aiba H., Mori K., Tanaka M., Ooi T., Roy A., Danchin A.
Nucleic Acids Res. 12:9427-9440(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Transcription of the Escherichia coli adenylate cyclase gene is negatively regulated by cAMP-cAMP receptor protein."
Aiba H.
J. Biol. Chem. 260:3063-3070(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
[3]"Comparative analysis of the cya locus in enterobacteria and related Gram-negative facultative anaerobes."
Trotot P., Sismeiro O., Vivares C., Glaser P., Bresson-Roy A., Danchin A.
Biochimie 78:277-287(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[6]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]"Regulation of adenylate cyclase synthesis in Escherichia coli: nucleotide sequence of the control region."
Roy A., Haziza C., Danchin A.
EMBO J. 2:791-797(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-183.
[8]"Cloning and promoter analysis of the Escherichia coli adenylate cyclase gene."
Aiba H., Kawamukai M., Ishihama A.
Nucleic Acids Res. 11:3451-3465(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 27-88.
[9]"Post-transcriptional control of Crp-cAMP by RNase LS in Escherichia coli."
Iwamoto A., Lemire S., Yonesaki T.
Mol. Microbiol. 70:1570-1578(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, REGULATION, DISRUPTION PHENOTYPE.
Strain: K12.
[10]"Phylogeny of adenylyl cyclases."
Danchin A.
Adv. Second Messenger Phosphoprotein Res. 27:109-162(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X01653 Genomic DNA. Translation: CAA25817.1.
X66782 Genomic DNA. Translation: CAA47280.1.
M87049 Genomic DNA. Translation: AAA67602.1.
U00096 Genomic DNA. Translation: AAC76809.1.
AP009048 Genomic DNA. Translation: BAE77495.1.
PIROYEC. G65184.
RefSeqNP_418250.1. NC_000913.3.
YP_491636.1. NC_007779.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP00936. 23 interactions.
STRING511145.b3806.

PTM databases

PhosSiteP0809376.

Proteomic databases

PaxDbP00936.
PRIDEP00936.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76809; AAC76809; b3806.
BAE77495; BAE77495; BAE77495.
GeneID12933616.
947755.
KEGGecj:Y75_p3372.
eco:b3806.
PATRIC32123111. VBIEscCol129921_3921.

Organism-specific databases

EchoBASEEB0168.
EcoGeneEG10170. cyaA.

Phylogenomic databases

eggNOGCOG3072.
HOGENOMHOG000270910.
KOK05851.
OMASEGFLQF.
OrthoDBEOG63JR9H.

Enzyme and pathway databases

BioCycEcoCyc:ADENYLATECYC-MONOMER.
ECOL316407:JW3778-MONOMER.
MetaCyc:ADENYLATECYC-MONOMER.

Gene expression databases

GenevestigatorP00936.

Family and domain databases

InterProIPR000274. Adenylate_cyclase_1.
IPR024686. Adenylate_cyclase_1_CS.
IPR024685. Adenylate_cyclase_1_N.
[Graphical view]
PfamPF12633. Adenyl_cycl_N. 1 hit.
PF01295. Adenylate_cycl. 1 hit.
[Graphical view]
PIRSFPIRSF001444. Adenylate_cycl. 1 hit.
PROSITEPS01092. ADENYLATE_CYCLASE_1_1. 1 hit.
PS01093. ADENYLATE_CYCLASE_1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP00936.

Entry information

Entry nameCYAA_ECOLI
AccessionPrimary (citable) accession number: P00936
Secondary accession number(s): Q2M8B1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 20, 2003
Last modified: June 11, 2014
This is version 122 of the entry and version 5 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene