P00936 (CYAA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylate cyclase
EC=4.6.1.1

Alternative name(s):
ATP pyrophosphate-lyase
Adenylyl cyclase

Gene names

Name:	cyaA
Synonyms:	cya
Ordered Locus Names:	b3806, JW3778

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	848 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate.
Enzyme regulation	The regulatory domain is involved in the regulation of cyclase activity by the carbon source. Activated by the PTS system, glucose-specific IIA component (CRR).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the adenylyl cyclase class-1 family.

Ontologies

Keywords
Biological process	cAMP biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Lyase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Inferred from direct assay. Source: EcoCyc protein binding Inferred from physical interaction. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 848	848	Adenylate cyclase		PRO_0000195669
Regions
	Region	1 – 535	535	Catalytic
	Region	541 – 848	308	Regulatory
Amino acid modifications
	Modified residue	609	1	Phosphohistidine; by CRR Potential
Experimental info
	Sequence conflict	223	1	V → G in CAA25817. Ref.1
	Sequence conflict	223	1	V → G in CAA47280. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P00936 [UniParc]. Last modified June 20, 2003. Version 5. Checksum: F0400E3406B15018	FASTA	848	97,586
	10 20 30 40 50 60 MYLYIETLKQ RLDAINQLRV DRALAAMGPA FQQVYSLLPT LLHYHHPLMP GYLDGNVPKG 70 80 90 100 110 120 ICLYTPDETQ RHYLNELELY RGMSVQDPPK GELPITGVYT MGSTSSVGQS CSSDLDIWVC 130 140 150 160 170 180 HQSWLDSEER QLLQRKCSLL ENWAASLGVE VSFFLIDENR FRHNESGSLG GEDCGSTQHI 190 200 210 220 230 240 LLLDEFYRTA VRLAGKRILW NMVPCDEEEH YDDYVMTLYA QGVLTPNEWL DLGGLSSLSA 250 260 270 280 290 300 EEYFGASLWQ LYKSIDSPYK AVLKTLLLEA YSWEYPNPRL LAKDIKQRLH DGEIVSFGLD 310 320 330 340 350 360 PYCMMLERVT EYLTAIEDFT RLDLVRRCFY LKVCEKLSRE RACVGWRRAV LSQLVSEWGW 370 380 390 400 410 420 DEARLAMLDN RANWKIDQVR EAHNELLDAM MQSYRNLIRF ARRNNLSVSA SPQDIGVLTR 430 440 450 460 470 480 KLYAAFEALP GKVTLVNPQI SPDLSEPNLT FIYVPPGRAN RSGWYLYNRA PNIESIISHQ 490 500 510 520 530 540 PLEYNRYLNK LVAWAWFNGL LTSRTRLYIK GNGIVDLPKL QEMVADVSHH FPLRLPAPTP 550 560 570 580 590 600 KALYSPCEIR HLAIIVNLEY DPTAAFRNQV VHFDFRKLDV FSFGENQNCL VGSVDLLYRN 610 620 630 640 650 660 SWNEVRTLHF NGEQSMIEAL KTILGKMHQD AAPPDSVEVF CYSQHLRGLI RTRVQQLVSE 670 680 690 700 710 720 CIELRLSSTR QETGRFKALR VSGQTWGLFF ERLNVSVQKL ENAIEFYGAI SHNKLHGLSV 730 740 750 760 770 780 QVETNHVKLP AVVDGFASEG IIQFFFEETQ DENGFNIYIL DESNRVEVYH HCEGSKEELV 790 800 810 820 830 840 RDVSRFYSSS HDRFTYGSSF INFNLPQFYQ IVKVDGREQV IPFRTKSIGN MPPANQDHDT PLLQQYFS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete nucleotide sequence of the adenylate cyclase gene of Escherichia coli." Aiba H., Mori K., Tanaka M., Ooi T., Roy A., Danchin A. Nucleic Acids Res. 12:9427-9440(1984) [PubMed: 6393056] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Transcription of the Escherichia coli adenylate cyclase gene is negatively regulated by cAMP-cAMP receptor protein." Aiba H. J. Biol. Chem. 260:3063-3070(1985) [PubMed: 2982847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Comparative analysis of the cya locus in enterobacteria and related Gram-negative facultative anaerobes." Trotot P., Sismeiro O., Vivares C., Glaser P., Bresson-Roy A., Danchin A. Biochimie 78:277-287(1996) [PubMed: 8874804] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R. Science 257:771-778(1992) [PubMed: 1379743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"Regulation of adenylate cyclase synthesis in Escherichia coli: nucleotide sequence of the control region." Roy A., Haziza C., Danchin A. EMBO J. 2:791-797(1983) [PubMed: 6357786] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-183.
[8]	"Cloning and promoter analysis of the Escherichia coli adenylate cyclase gene." Aiba H., Kawamukai M., Ishihama A. Nucleic Acids Res. 11:3451-3465(1983) [PubMed: 6344011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 27-88.
[9]	"Phylogeny of adenylyl cyclases." Danchin A. Adv. Second Messenger Phosphoprotein Res. 27:109-162(1993) [PubMed: 8418825] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Web resources

Escherichia coli adenylate cyclase homepage

Cross-references

Sequence databases
EMBL GenBank DDBJ	X01653 Genomic DNA. Translation: CAA25817.1. X66782 Genomic DNA. Translation: CAA47280.1. M87049 Genomic DNA. Translation: AAA67602.1. U00096 Genomic DNA. Translation: AAC76809.1. AP009048 Genomic DNA. Translation: BAE77495.1.
PIR	OYEC. G65184.
RefSeq	NP_418250.1. NC_000913.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P00936. 13 interactions.
PTM databases
PhosSite	P00936.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002806; EBESCP00000002806; EBESCG00000002291. EBESCT00000002807; EBESCP00000002807; EBESCG00000002291. EBESCT00000002808; EBESCP00000002808; EBESCG00000002291. EBESCT00000017138; EBESCP00000016429; EBESCG00000016197.
GeneID	947755.
GenomeReviews	Gene locus JW3778 in contig AP009048_GR. Gene locus b3806 in contig U00096_GR.
KEGG	ecj:JW3778. eco:b3806.
PATRIC	32123111. VBIEscCol129921_3921.
Organism-specific databases
EchoBASE	EB0168.
EcoGene	EG10170. cyaA.
Phylogenomic databases
eggNOG	COG3072.
HOGENOM	HBG678054.
KO	K05851.
OMA	LWQLYKG.
ProtClustDB	PRK09450.
Enzyme and pathway databases
BioCyc	EcoCyc:ADENYLATECYC-MONOMER. MetaCyc:ADENYLATECYC-MONOMER.
Gene expression databases
Genevestigator	P00936.
Family and domain databases
InterPro	IPR000274. Adenylate_cyclase_1. IPR024686. Adenylate_cyclase_1_CS. IPR024685. Adenylate_cyclase_1_N. [Graphical view]
Pfam	PF12633. Adenyl_cycl_N. 1 hit. PF01295. Adenylate_cycl. 1 hit. [Graphical view]
PIRSF	PIRSF001444. Adenylate_cycl. 1 hit.
PROSITE	PS01092. ADENYLATE_CYCLASE_1_1. 1 hit. PS01093. ADENYLATE_CYCLASE_1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYAA_ECOLI

Accession

Primary (citable) accession number: P00936
Secondary accession number(s): Q2M8B1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	June 20, 2003
Last modified:	February 22, 2012
This is version 105 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program