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Protein

Tryptophan synthase

Gene

TRP5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 15500 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.

Cofactori

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Multifunctional tryptophan biosynthesis protein (TRP3), Anthranilate synthase component 1 (TRP2)
  2. Anthranilate phosphoribosyltransferase (TRP4)
  3. N-(5'-phosphoribosyl)anthranilate isomerase (TRP1)
  4. Multifunctional tryptophan biosynthesis protein (TRP3)
  5. Tryptophan synthase (TRP5)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei50Proton acceptorBy similarity1
Active sitei61Proton acceptorBy similarity1

GO - Molecular functioni

  • tryptophan synthase activity Source: SGD

GO - Biological processi

  • tryptophan biosynthetic process Source: SGD

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:YGL026C-MONOMER
UniPathwayiUPA00035; UER00044

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase (EC:4.2.1.20)
Gene namesi
Name:TRP5
Ordered Locus Names:YGL026C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL026C
SGDiS000002994 TRP5

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000987261 – 707Tryptophan synthaseAdd BLAST707

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei384N6-(pyridoxal phosphate)lysineBy similarity1
Modified residuei540PhosphoserineCombined sources1
Modified residuei683PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP00931
PaxDbiP00931
PRIDEiP00931

PTM databases

iPTMnetiP00931

Interactioni

Protein-protein interaction databases

BioGridi33221, 30 interactors
DIPiDIP-1398N
IntActiP00931, 12 interactors
MINTiP00931
STRINGi4932.YGL026C

Structurei

3D structure databases

ProteinModelPortaliP00931
SMRiP00931
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 297Tryptophan synthase alpha chainAdd BLAST297
Regioni298 – 707Tryptophan synthase beta chainAdd BLAST410

Sequence similaritiesi

In the N-terminal section; belongs to the TrpA family.Curated
In the C-terminal section; belongs to the TrpB family.Curated

Phylogenomic databases

HOGENOMiHOG000161710
InParanoidiP00931
KOiK01694
OMAiCAKFGME
OrthoDBiEOG092C0Y7R

Family and domain databases

CDDicd06446 Trp-synth_B, 1 hit
cd04724 Tryptophan_synthase_alpha, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00131 Trp_synth_alpha, 1 hit
MF_00133 Trp_synth_beta, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR001926 PLP-dep
IPR011060 RibuloseP-bd_barrel
IPR006653 Trp_synth_b_CS
IPR006654 Trp_synth_beta
IPR023026 Trp_synth_beta/beta-like
IPR018204 Trp_synthase_alpha_AS
IPR002028 Trp_synthase_suA
IPR036052 Trypto_synt_PLP_dependent
PANTHERiPTHR42882 PTHR42882, 1 hit
PfamiView protein in Pfam
PF00291 PALP, 1 hit
PF00290 Trp_syntA, 1 hit
SUPFAMiSSF51366 SSF51366, 1 hit
SSF53686 SSF53686, 1 hit
TIGRFAMsiTIGR00262 trpA, 1 hit
TIGR00263 trpB, 1 hit
PROSITEiView protein in PROSITE
PS00167 TRP_SYNTHASE_ALPHA, 1 hit
PS00168 TRP_SYNTHASE_BETA, 1 hit

Sequencei

Sequence statusi: Complete.

P00931-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEQLRQTFA NAKKENRNAL VTFMTAGYPT VKDTVPILKG FQDGGVDIIE
60 70 80 90 100
LGMPFSDPIA DGPTIQLSNT VALQNGVTLP QTLEMVSQAR NEGVTVPIIL
110 120 130 140 150
MGYYNPILNY GEERFIQDAA KAGANGFIIV DLPPEEALKV RNYINDNGLS
160 170 180 190 200
LIPLVAPSTT DERLELLSHI ADSFVYVVSR MGTTGVQSSV ASDLDELISR
210 220 230 240 250
VRKYTKDTPL AVGFGVSTRE HFQSVGSVAD GVVIGSKIVT LCGDAPEGKR
260 270 280 290 300
YDVAKEYVQG ILNGAKHKVL SKDEFFAFQK ESLKSANVKK EILDEFDENH
310 320 330 340 350
KHPIRFGDFG GQYVPEALHA CLRELEKGFD EAVADPTFWE DFKSLYSYIG
360 370 380 390 400
RPSSLHKAER LTEHCQGAQI WLKREDLNHT GSHKINNALA QVLLAKRLGK
410 420 430 440 450
KNVIAETGAG QHGVATATAC AKFGLTCTVF MGAEDVRRQA LNVFRMRILG
460 470 480 490 500
AKVIAVTNGT KTLRDATSEA FRFWVTNLKT TYYVVGSAIG PHPYPTLVRT
510 520 530 540 550
FQSVIGKETK EQFAAMNNGK LPDAVVACVG GGSNSTGMFS PFEHDTSVKL
560 570 580 590 600
LGVEAGGDGV DTKFHSATLT AGRPGVFHGV KTYVLQDSDG QVHDTHSVSA
610 620 630 640 650
GLDYPGVGPE LAYWKSTGRA QFIAATDAQA LLGFKLLSQL EGIIPALESS
660 670 680 690 700
HAVYGACELA KTMKPDQHLV INISGRGDKD VQSVAEVLPK LGPKIGWDLR

FEEDPSA
Length:707
Mass (Da):76,626
Last modified:July 21, 1986 - v1
Checksum:iF5241A0127A2D166
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01342 Genomic DNA Translation: CAA24635.1
V01343 Genomic DNA Translation: CAA24636.1
Z72548 Genomic DNA Translation: CAA96727.1
BK006941 Genomic DNA Translation: DAA08073.1
PIRiA01154 TSBYAB
RefSeqiNP_011489.1, NM_001180891.1

Genome annotation databases

EnsemblFungiiYGL026C; YGL026C; YGL026C
GeneIDi852858
KEGGisce:YGL026C

Similar proteinsi

Entry informationi

Entry nameiTRP_YEAST
AccessioniPrimary (citable) accession number: P00931
Secondary accession number(s): D6VUB2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 23, 2018
This is version 181 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health