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P00931

- TRP_YEAST

UniProt

P00931 - TRP_YEAST

Protein

Tryptophan synthase

Gene

TRP5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.

    Cofactori

    Pyridoxal phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei50 – 501Proton acceptorBy similarity
    Active sitei61 – 611Proton acceptorBy similarity

    GO - Molecular functioni

    1. tryptophan synthase activity Source: SGD

    GO - Biological processi

    1. tryptophan biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciYEAST:YGL026C-MONOMER.
    UniPathwayiUPA00035; UER00044.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tryptophan synthase (EC:4.2.1.20)
    Gene namesi
    Name:TRP5
    Ordered Locus Names:YGL026C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL026c.
    SGDiS000002994. TRP5.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 707707Tryptophan synthasePRO_0000098726Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei384 – 3841N6-(pyridoxal phosphate)lysineBy similarity
    Modified residuei540 – 5401Phosphoserine1 Publication
    Modified residuei683 – 6831Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP00931.
    PaxDbiP00931.
    PeptideAtlasiP00931.
    PRIDEiP00931.

    Expressioni

    Gene expression databases

    GenevestigatoriP00931.

    Interactioni

    Protein-protein interaction databases

    BioGridi33221. 21 interactions.
    DIPiDIP-1398N.
    IntActiP00931. 7 interactions.
    MINTiMINT-405390.
    STRINGi4932.YGL026C.

    Structurei

    3D structure databases

    ProteinModelPortaliP00931.
    SMRiP00931. Positions 19-244, 306-687.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 297297Tryptophan synthase alpha chainAdd
    BLAST
    Regioni298 – 707410Tryptophan synthase beta chainAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the TrpA family.Curated
    In the C-terminal section; belongs to the TrpB family.Curated

    Phylogenomic databases

    eggNOGiCOG0133.
    HOGENOMiHOG000161710.
    KOiK01694.
    OMAiPGVFHGV.
    OrthoDBiEOG7D864Z.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00131. Trp_synth_alpha.
    MF_00133. Trp_synth_beta.
    InterProiIPR013785. Aldolase_TIM.
    IPR011060. RibuloseP-bd_barrel.
    IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
    IPR006653. Trp_synth_b_CS.
    IPR006654. Trp_synth_beta.
    IPR023026. Trp_synth_beta/beta-like.
    IPR018204. Trp_synthase_alpha_AS.
    IPR002028. Trp_synthase_suA.
    [Graphical view]
    PANTHERiPTHR10314:SF3. PTHR10314:SF3. 1 hit.
    PfamiPF00291. PALP. 1 hit.
    PF00290. Trp_syntA. 1 hit.
    [Graphical view]
    SUPFAMiSSF51366. SSF51366. 1 hit.
    SSF53686. SSF53686. 1 hit.
    TIGRFAMsiTIGR00262. trpA. 1 hit.
    TIGR00263. trpB. 1 hit.
    PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
    PS00168. TRP_SYNTHASE_BETA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00931-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEQLRQTFA NAKKENRNAL VTFMTAGYPT VKDTVPILKG FQDGGVDIIE    50
    LGMPFSDPIA DGPTIQLSNT VALQNGVTLP QTLEMVSQAR NEGVTVPIIL 100
    MGYYNPILNY GEERFIQDAA KAGANGFIIV DLPPEEALKV RNYINDNGLS 150
    LIPLVAPSTT DERLELLSHI ADSFVYVVSR MGTTGVQSSV ASDLDELISR 200
    VRKYTKDTPL AVGFGVSTRE HFQSVGSVAD GVVIGSKIVT LCGDAPEGKR 250
    YDVAKEYVQG ILNGAKHKVL SKDEFFAFQK ESLKSANVKK EILDEFDENH 300
    KHPIRFGDFG GQYVPEALHA CLRELEKGFD EAVADPTFWE DFKSLYSYIG 350
    RPSSLHKAER LTEHCQGAQI WLKREDLNHT GSHKINNALA QVLLAKRLGK 400
    KNVIAETGAG QHGVATATAC AKFGLTCTVF MGAEDVRRQA LNVFRMRILG 450
    AKVIAVTNGT KTLRDATSEA FRFWVTNLKT TYYVVGSAIG PHPYPTLVRT 500
    FQSVIGKETK EQFAAMNNGK LPDAVVACVG GGSNSTGMFS PFEHDTSVKL 550
    LGVEAGGDGV DTKFHSATLT AGRPGVFHGV KTYVLQDSDG QVHDTHSVSA 600
    GLDYPGVGPE LAYWKSTGRA QFIAATDAQA LLGFKLLSQL EGIIPALESS 650
    HAVYGACELA KTMKPDQHLV INISGRGDKD VQSVAEVLPK LGPKIGWDLR 700
    FEEDPSA 707
    Length:707
    Mass (Da):76,626
    Last modified:July 21, 1986 - v1
    Checksum:iF5241A0127A2D166
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01342 Genomic DNA. Translation: CAA24635.1.
    V01343 Genomic DNA. Translation: CAA24636.1.
    Z72548 Genomic DNA. Translation: CAA96727.1.
    BK006941 Genomic DNA. Translation: DAA08073.1.
    PIRiA01154. TSBYAB.
    RefSeqiNP_011489.1. NM_001180891.1.

    Genome annotation databases

    EnsemblFungiiYGL026C; YGL026C; YGL026C.
    GeneIDi852858.
    KEGGisce:YGL026C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01342 Genomic DNA. Translation: CAA24635.1 .
    V01343 Genomic DNA. Translation: CAA24636.1 .
    Z72548 Genomic DNA. Translation: CAA96727.1 .
    BK006941 Genomic DNA. Translation: DAA08073.1 .
    PIRi A01154. TSBYAB.
    RefSeqi NP_011489.1. NM_001180891.1.

    3D structure databases

    ProteinModelPortali P00931.
    SMRi P00931. Positions 19-244, 306-687.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33221. 21 interactions.
    DIPi DIP-1398N.
    IntActi P00931. 7 interactions.
    MINTi MINT-405390.
    STRINGi 4932.YGL026C.

    Proteomic databases

    MaxQBi P00931.
    PaxDbi P00931.
    PeptideAtlasi P00931.
    PRIDEi P00931.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL026C ; YGL026C ; YGL026C .
    GeneIDi 852858.
    KEGGi sce:YGL026C.

    Organism-specific databases

    CYGDi YGL026c.
    SGDi S000002994. TRP5.

    Phylogenomic databases

    eggNOGi COG0133.
    HOGENOMi HOG000161710.
    KOi K01694.
    OMAi PGVFHGV.
    OrthoDBi EOG7D864Z.

    Enzyme and pathway databases

    UniPathwayi UPA00035 ; UER00044 .
    BioCyci YEAST:YGL026C-MONOMER.

    Miscellaneous databases

    NextBioi 972463.

    Gene expression databases

    Genevestigatori P00931.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00131. Trp_synth_alpha.
    MF_00133. Trp_synth_beta.
    InterProi IPR013785. Aldolase_TIM.
    IPR011060. RibuloseP-bd_barrel.
    IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
    IPR006653. Trp_synth_b_CS.
    IPR006654. Trp_synth_beta.
    IPR023026. Trp_synth_beta/beta-like.
    IPR018204. Trp_synthase_alpha_AS.
    IPR002028. Trp_synthase_suA.
    [Graphical view ]
    PANTHERi PTHR10314:SF3. PTHR10314:SF3. 1 hit.
    Pfami PF00291. PALP. 1 hit.
    PF00290. Trp_syntA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51366. SSF51366. 1 hit.
    SSF53686. SSF53686. 1 hit.
    TIGRFAMsi TIGR00262. trpA. 1 hit.
    TIGR00263. trpB. 1 hit.
    PROSITEi PS00167. TRP_SYNTHASE_ALPHA. 1 hit.
    PS00168. TRP_SYNTHASE_BETA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Yeast gene TRP5: structure, function, regulation."
      Zalkin H., Yanofsky C.
      J. Biol. Chem. 257:1491-1500(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "DNA sequences flanking an E. coli insertion element IS2 in a cloned yeast TRP5 gene."
      Brosius J., Walz A.
      Gene 17:223-228(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-172.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-540 AND SER-683, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTRP_YEAST
    AccessioniPrimary (citable) accession number: P00931
    Secondary accession number(s): D6VUB2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 146 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 15500 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3