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P00931 (TRP_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 145. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan synthase

EC=4.2.1.20
Gene names
Name:TRP5
Ordered Locus Names:YGL026C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length707 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O. HAMAP-Rule MF_00131

Cofactor

Pyridoxal phosphate.

Pathway

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5. HAMAP-Rule MF_00131

Miscellaneous

Present with 15500 molecules/cell in log phase SD medium.

Sequence similarities

In the N-terminal section; belongs to the TrpA family.

In the C-terminal section; belongs to the TrpB family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 707707Tryptophan synthase HAMAP-Rule MF_00131
PRO_0000098726

Regions

Region1 – 297297Tryptophan synthase alpha chain HAMAP-Rule MF_00131
Region298 – 707410Tryptophan synthase beta chain HAMAP-Rule MF_00131

Sites

Active site501Proton acceptor By similarity
Active site611Proton acceptor By similarity

Amino acid modifications

Modified residue3841N6-(pyridoxal phosphate)lysine By similarity
Modified residue5401Phosphoserine Ref.6
Modified residue6831Phosphoserine Ref.6

Sequences

Sequence LengthMass (Da)Tools
P00931 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: F5241A0127A2D166

FASTA70776,626
        10         20         30         40         50         60 
MSEQLRQTFA NAKKENRNAL VTFMTAGYPT VKDTVPILKG FQDGGVDIIE LGMPFSDPIA 

        70         80         90        100        110        120 
DGPTIQLSNT VALQNGVTLP QTLEMVSQAR NEGVTVPIIL MGYYNPILNY GEERFIQDAA 

       130        140        150        160        170        180 
KAGANGFIIV DLPPEEALKV RNYINDNGLS LIPLVAPSTT DERLELLSHI ADSFVYVVSR 

       190        200        210        220        230        240 
MGTTGVQSSV ASDLDELISR VRKYTKDTPL AVGFGVSTRE HFQSVGSVAD GVVIGSKIVT 

       250        260        270        280        290        300 
LCGDAPEGKR YDVAKEYVQG ILNGAKHKVL SKDEFFAFQK ESLKSANVKK EILDEFDENH 

       310        320        330        340        350        360 
KHPIRFGDFG GQYVPEALHA CLRELEKGFD EAVADPTFWE DFKSLYSYIG RPSSLHKAER 

       370        380        390        400        410        420 
LTEHCQGAQI WLKREDLNHT GSHKINNALA QVLLAKRLGK KNVIAETGAG QHGVATATAC 

       430        440        450        460        470        480 
AKFGLTCTVF MGAEDVRRQA LNVFRMRILG AKVIAVTNGT KTLRDATSEA FRFWVTNLKT 

       490        500        510        520        530        540 
TYYVVGSAIG PHPYPTLVRT FQSVIGKETK EQFAAMNNGK LPDAVVACVG GGSNSTGMFS 

       550        560        570        580        590        600 
PFEHDTSVKL LGVEAGGDGV DTKFHSATLT AGRPGVFHGV KTYVLQDSDG QVHDTHSVSA 

       610        620        630        640        650        660 
GLDYPGVGPE LAYWKSTGRA QFIAATDAQA LLGFKLLSQL EGIIPALESS HAVYGACELA 

       670        680        690        700 
KTMKPDQHLV INISGRGDKD VQSVAEVLPK LGPKIGWDLR FEEDPSA 

« Hide

References

« Hide 'large scale' references
[1]"Yeast gene TRP5: structure, function, regulation."
Zalkin H., Yanofsky C.
J. Biol. Chem. 257:1491-1500(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"DNA sequences flanking an E. coli insertion element IS2 in a cloned yeast TRP5 gene."
Brosius J., Walz A.
Gene 17:223-228(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-172.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-540 AND SER-683, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V01342 Genomic DNA. Translation: CAA24635.1.
V01343 Genomic DNA. Translation: CAA24636.1.
Z72548 Genomic DNA. Translation: CAA96727.1.
BK006941 Genomic DNA. Translation: DAA08073.1.
PIRTSBYAB. A01154.
RefSeqNP_011489.1. NM_001180891.1.

3D structure databases

ProteinModelPortalP00931.
SMRP00931. Positions 19-244, 306-687.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33221. 21 interactions.
DIPDIP-1398N.
IntActP00931. 7 interactions.
MINTMINT-405390.
STRING4932.YGL026C.

Proteomic databases

MaxQBP00931.
PaxDbP00931.
PeptideAtlasP00931.
PRIDEP00931.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL026C; YGL026C; YGL026C.
GeneID852858.
KEGGsce:YGL026C.

Organism-specific databases

CYGDYGL026c.
SGDS000002994. TRP5.

Phylogenomic databases

eggNOGCOG0133.
HOGENOMHOG000161710.
KOK01694.
OMAPGVFHGV.
OrthoDBEOG7D864Z.

Enzyme and pathway databases

BioCycYEAST:YGL026C-MONOMER.
UniPathwayUPA00035; UER00044.

Gene expression databases

GenevestigatorP00931.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00131. Trp_synth_alpha.
MF_00133. Trp_synth_beta.
InterProIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
IPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PANTHERPTHR10314:SF3. PTHR10314:SF3. 1 hit.
PfamPF00291. PALP. 1 hit.
PF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMSSF51366. SSF51366. 1 hit.
SSF53686. SSF53686. 1 hit.
TIGRFAMsTIGR00262. trpA. 1 hit.
TIGR00263. trpB. 1 hit.
PROSITEPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
PS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972463.

Entry information

Entry nameTRP_YEAST
AccessionPrimary (citable) accession number: P00931
Secondary accession number(s): D6VUB2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 14, 2014
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways