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Protein

Tryptophan synthase

Gene

TRP5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.

Cofactori

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei50 – 501Proton acceptorBy similarity
Active sitei61 – 611Proton acceptorBy similarity

GO - Molecular functioni

  1. tryptophan synthase activity Source: SGD

GO - Biological processi

  1. tryptophan biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:YGL026C-MONOMER.
UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase (EC:4.2.1.20)
Gene namesi
Name:TRP5
Ordered Locus Names:YGL026C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VII

Organism-specific databases

CYGDiYGL026c.
EuPathDBiFungiDB:YGL026C.
SGDiS000002994. TRP5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 707707Tryptophan synthasePRO_0000098726Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei384 – 3841N6-(pyridoxal phosphate)lysineBy similarity
Modified residuei540 – 5401Phosphoserine1 Publication
Modified residuei683 – 6831Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP00931.
PaxDbiP00931.
PeptideAtlasiP00931.
PRIDEiP00931.

Expressioni

Gene expression databases

GenevestigatoriP00931.

Interactioni

Protein-protein interaction databases

BioGridi33221. 23 interactions.
DIPiDIP-1398N.
IntActiP00931. 8 interactions.
MINTiMINT-405390.
STRINGi4932.YGL026C.

Structurei

3D structure databases

ProteinModelPortaliP00931.
SMRiP00931. Positions 19-244, 306-687.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 297297Tryptophan synthase alpha chainAdd
BLAST
Regioni298 – 707410Tryptophan synthase beta chainAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the TrpA family.Curated
In the C-terminal section; belongs to the TrpB family.Curated

Phylogenomic databases

eggNOGiCOG0133.
HOGENOMiHOG000161710.
InParanoidiP00931.
KOiK01694.
OMAiPGVFHGV.
OrthoDBiEOG7D864Z.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha.
MF_00133. Trp_synth_beta.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PANTHERiPTHR10314:SF3. PTHR10314:SF3. 1 hit.
PfamiPF00291. PALP. 1 hit.
PF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
SSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
TIGR00263. trpB. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
PS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00931-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEQLRQTFA NAKKENRNAL VTFMTAGYPT VKDTVPILKG FQDGGVDIIE
60 70 80 90 100
LGMPFSDPIA DGPTIQLSNT VALQNGVTLP QTLEMVSQAR NEGVTVPIIL
110 120 130 140 150
MGYYNPILNY GEERFIQDAA KAGANGFIIV DLPPEEALKV RNYINDNGLS
160 170 180 190 200
LIPLVAPSTT DERLELLSHI ADSFVYVVSR MGTTGVQSSV ASDLDELISR
210 220 230 240 250
VRKYTKDTPL AVGFGVSTRE HFQSVGSVAD GVVIGSKIVT LCGDAPEGKR
260 270 280 290 300
YDVAKEYVQG ILNGAKHKVL SKDEFFAFQK ESLKSANVKK EILDEFDENH
310 320 330 340 350
KHPIRFGDFG GQYVPEALHA CLRELEKGFD EAVADPTFWE DFKSLYSYIG
360 370 380 390 400
RPSSLHKAER LTEHCQGAQI WLKREDLNHT GSHKINNALA QVLLAKRLGK
410 420 430 440 450
KNVIAETGAG QHGVATATAC AKFGLTCTVF MGAEDVRRQA LNVFRMRILG
460 470 480 490 500
AKVIAVTNGT KTLRDATSEA FRFWVTNLKT TYYVVGSAIG PHPYPTLVRT
510 520 530 540 550
FQSVIGKETK EQFAAMNNGK LPDAVVACVG GGSNSTGMFS PFEHDTSVKL
560 570 580 590 600
LGVEAGGDGV DTKFHSATLT AGRPGVFHGV KTYVLQDSDG QVHDTHSVSA
610 620 630 640 650
GLDYPGVGPE LAYWKSTGRA QFIAATDAQA LLGFKLLSQL EGIIPALESS
660 670 680 690 700
HAVYGACELA KTMKPDQHLV INISGRGDKD VQSVAEVLPK LGPKIGWDLR

FEEDPSA
Length:707
Mass (Da):76,626
Last modified:July 21, 1986 - v1
Checksum:iF5241A0127A2D166
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01342 Genomic DNA. Translation: CAA24635.1.
V01343 Genomic DNA. Translation: CAA24636.1.
Z72548 Genomic DNA. Translation: CAA96727.1.
BK006941 Genomic DNA. Translation: DAA08073.1.
PIRiA01154. TSBYAB.
RefSeqiNP_011489.1. NM_001180891.1.

Genome annotation databases

EnsemblFungiiYGL026C; YGL026C; YGL026C.
GeneIDi852858.
KEGGisce:YGL026C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01342 Genomic DNA. Translation: CAA24635.1.
V01343 Genomic DNA. Translation: CAA24636.1.
Z72548 Genomic DNA. Translation: CAA96727.1.
BK006941 Genomic DNA. Translation: DAA08073.1.
PIRiA01154. TSBYAB.
RefSeqiNP_011489.1. NM_001180891.1.

3D structure databases

ProteinModelPortaliP00931.
SMRiP00931. Positions 19-244, 306-687.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33221. 23 interactions.
DIPiDIP-1398N.
IntActiP00931. 8 interactions.
MINTiMINT-405390.
STRINGi4932.YGL026C.

Proteomic databases

MaxQBiP00931.
PaxDbiP00931.
PeptideAtlasiP00931.
PRIDEiP00931.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL026C; YGL026C; YGL026C.
GeneIDi852858.
KEGGisce:YGL026C.

Organism-specific databases

CYGDiYGL026c.
EuPathDBiFungiDB:YGL026C.
SGDiS000002994. TRP5.

Phylogenomic databases

eggNOGiCOG0133.
HOGENOMiHOG000161710.
InParanoidiP00931.
KOiK01694.
OMAiPGVFHGV.
OrthoDBiEOG7D864Z.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.
BioCyciYEAST:YGL026C-MONOMER.

Miscellaneous databases

NextBioi972463.
PROiP00931.

Gene expression databases

GenevestigatoriP00931.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha.
MF_00133. Trp_synth_beta.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PANTHERiPTHR10314:SF3. PTHR10314:SF3. 1 hit.
PfamiPF00291. PALP. 1 hit.
PF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
SSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
TIGR00263. trpB. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
PS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast gene TRP5: structure, function, regulation."
    Zalkin H., Yanofsky C.
    J. Biol. Chem. 257:1491-1500(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "DNA sequences flanking an E. coli insertion element IS2 in a cloned yeast TRP5 gene."
    Brosius J., Walz A.
    Gene 17:223-228(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-172.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-540 AND SER-683, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTRP_YEAST
AccessioniPrimary (citable) accession number: P00931
Secondary accession number(s): D6VUB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 29, 2015
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 15500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.