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P00927 (THDH_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Threonine dehydratase, mitochondrial

EC=4.3.1.19
Alternative name(s):
Threonine deaminase
Gene names
Name:ILV1
Ordered Locus Names:YER086W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

L-threonine = 2-oxobutanoate + NH3. Ref.5

Cofactor

Pyridoxal phosphate.

Enzyme regulation

Isoleucine allosterically inhibits while valine allosterically activates this enzyme. Ref.4

Pathway

Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine: step 1/1.

Subunit structure

Homotetramer.

Subcellular location

Mitochondrion Ref.6 Ref.8 Ref.9.

Induction

In response to starvation for tryptophan and branched-chain amino acid imbalance. Ref.4 Ref.5

Miscellaneous

Present with 11900 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the serine/threonine dehydratase family.

Contains 2 ACT-like domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 576Threonine dehydratase, mitochondrialPRO_0000033616

Regions

Domain393 – 47381ACT-like 1
Domain495 – 56672ACT-like 2

Amino acid modifications

Modified residue1091N6-(pyridoxal phosphate)lysine By similarity

Experimental info

Sequence conflict2591I → T in AAA34705. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P00927 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 0801BCBD7EEDDC1F

FASTA57663,831
        10         20         30         40         50         60 
MSATLLKQPL CTVVRQGKQS KVSGLNLLRL KAHLHRQHLS PSLIKLHSEL KLDELQTDNT 

        70         80         90        100        110        120 
PDYVRLVLRS SVYDVINESP ISQGVGLSSR LNTNVILKRE DLLPVFSFKL RGAYNMIAKL 

       130        140        150        160        170        180 
DDSQRNQGVI ACSAGNHAQG VAFAAKHLKI PATIVMPVCT PSIKYQNVSR LGSQVVLYGN 

       190        200        210        220        230        240 
DFDEAKAECA KLAEERGLTN IPPFDHPYVI AGQGTVAMEI LRQVRTANKI GAVFVPVGGG 

       250        260        270        280        290        300 
GLIAGIGAYL KRVAPHIKII GVETYDAATL HNSLQRNQRT PLPVVGTFAD GTSVRMIGEE 

       310        320        330        340        350        360 
TFRVAQQVVD EVVLVNTDEI CAAVKDIFED TRSIVEPSGA LSVAGMKKYI STVHPEIDHT 

       370        380        390        400        410        420 
KNTYVPILSG ANMNFDRLRF VSERAVLGEG KEVFMLVTLP DVPGAFKKMQ KIIHPRSVTE 

       430        440        450        460        470        480 
FSYRYNEHRH ESSSEVPKAY IYTSFSVVDR EKEIKQVMQQ LNALGFEAVD ISDNELAKSH 

       490        500        510        520        530        540 
GRYLVGGASK VPNERIISFE FPERPGALTR FLGGLSDSWN LTLFHYRNHG ADIGKVLAGI 

       550        560        570 
SVPPRENLTF QKFLEDLGYT YHDETDNTVY QKFLKY 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the gene for threonine deaminase (ILV1) of Saccharomyces cerevisiae."
Kielland-Brandt M.C., Holmberg S., Petersen J.G.L., Nilsson-Tillgren T.
Carlsberg Res. Commun. 49:567-575(1984)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Purification and properties of threonine deaminase from Saccharomyces cerevisiae."
Ahmed S.I., Bollon A.P., Rogers S.J., Magee P.T.
Biochimie 58:225-232(1976) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
[5]"Regulation of isoleucine-valine biosynthesis in Saccharomyces cerevisiae."
Holmberg S., Petersen J.G.
Curr. Genet. 13:207-217(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME ACTIVITY, INDUCTION.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
[9]"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M36383 Genomic DNA. Translation: AAA34705.1.
X01466 Genomic DNA. Translation: CAA25696.1.
U18839 Genomic DNA. Translation: AAB64641.1.
BK006939 Genomic DNA. Translation: DAA07746.1.
PIRDWBYT. S50589.
RefSeqNP_011009.1. NM_001178977.1.

3D structure databases

ProteinModelPortalP00927.
SMRP00927. Positions 57-388.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36830. 204 interactions.
DIPDIP-4029N.
IntActP00927. 34 interactions.
MINTMINT-565661.
STRING4932.YER086W.

Proteomic databases

MaxQBP00927.
PaxDbP00927.
PeptideAtlasP00927.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER086W; YER086W; YER086W.
GeneID856819.
KEGGsce:YER086W.

Organism-specific databases

CYGDYER086w.
SGDS000000888. ILV1.

Phylogenomic databases

eggNOGCOG1171.
GeneTreeENSGT00600000084626.
HOGENOMHOG000046975.
KOK01754.
OMAATLEYSS.
OrthoDBEOG75XGVN.

Enzyme and pathway databases

BioCycYEAST:YER086W-MONOMER.
UniPathwayUPA00047; UER00054.

Gene expression databases

GenevestigatorP00927.

Family and domain databases

InterProIPR001721. ACT-like_dom.
IPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
IPR005787. Thr_deHydtase_biosynth.
IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
[Graphical view]
PfamPF00291. PALP. 1 hit.
PF00585. Thr_dehydrat_C. 2 hits.
[Graphical view]
SUPFAMSSF53686. SSF53686. 1 hit.
TIGRFAMsTIGR01124. ilvA_2Cterm. 1 hit.
PROSITEPS51672. ACT_LIKE. 2 hits.
PS00165. DEHYDRATASE_SER_THR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio983097.
PROP00927.

Entry information

Entry nameTHDH_YEAST
AccessionPrimary (citable) accession number: P00927
Secondary accession number(s): D3DLZ2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways