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P00927

- THDH_YEAST

UniProt

P00927 - THDH_YEAST

Protein

Threonine dehydratase, mitochondrial

Gene

ILV1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    L-threonine = 2-oxobutanoate + NH3.1 Publication

    Cofactori

    Pyridoxal phosphate.

    Enzyme regulationi

    Isoleucine allosterically inhibits while valine allosterically activates this enzyme.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. L-threonine ammonia-lyase activity Source: SGD
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. isoleucine biosynthetic process Source: SGD
    2. threonine catabolic process Source: SGD

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Isoleucine biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciYEAST:YER086W-MONOMER.
    UniPathwayiUPA00047; UER00054.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Threonine dehydratase, mitochondrial (EC:4.3.1.19)
    Alternative name(s):
    Threonine deaminase
    Gene namesi
    Name:ILV1
    Ordered Locus Names:YER086W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER086w.
    SGDiS000000888. ILV1.

    Subcellular locationi

    Mitochondrion 3 Publications

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 576Threonine dehydratase, mitochondrialPRO_0000033616
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei109 – 1091N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    MaxQBiP00927.
    PaxDbiP00927.
    PeptideAtlasiP00927.

    Expressioni

    Inductioni

    In response to starvation for tryptophan and branched-chain amino acid imbalance.1 Publication

    Gene expression databases

    GenevestigatoriP00927.

    Interactioni

    Subunit structurei

    Homotetramer.

    Protein-protein interaction databases

    BioGridi36830. 204 interactions.
    DIPiDIP-4029N.
    IntActiP00927. 34 interactions.
    MINTiMINT-565661.
    STRINGi4932.YER086W.

    Structurei

    3D structure databases

    ProteinModelPortaliP00927.
    SMRiP00927. Positions 57-388.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini393 – 47381ACT-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini495 – 56672ACT-like 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 ACT-like domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transit peptide

    Phylogenomic databases

    eggNOGiCOG1171.
    GeneTreeiENSGT00600000084626.
    HOGENOMiHOG000046975.
    KOiK01754.
    OMAiATLEYSS.
    OrthoDBiEOG75XGVN.

    Family and domain databases

    InterProiIPR001721. ACT-like_dom.
    IPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
    IPR005787. Thr_deHydtase_biosynth.
    IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
    [Graphical view]
    PfamiPF00291. PALP. 1 hit.
    PF00585. Thr_dehydrat_C. 2 hits.
    [Graphical view]
    SUPFAMiSSF53686. SSF53686. 1 hit.
    TIGRFAMsiTIGR01124. ilvA_2Cterm. 1 hit.
    PROSITEiPS51672. ACT_LIKE. 2 hits.
    PS00165. DEHYDRATASE_SER_THR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00927-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSATLLKQPL CTVVRQGKQS KVSGLNLLRL KAHLHRQHLS PSLIKLHSEL    50
    KLDELQTDNT PDYVRLVLRS SVYDVINESP ISQGVGLSSR LNTNVILKRE 100
    DLLPVFSFKL RGAYNMIAKL DDSQRNQGVI ACSAGNHAQG VAFAAKHLKI 150
    PATIVMPVCT PSIKYQNVSR LGSQVVLYGN DFDEAKAECA KLAEERGLTN 200
    IPPFDHPYVI AGQGTVAMEI LRQVRTANKI GAVFVPVGGG GLIAGIGAYL 250
    KRVAPHIKII GVETYDAATL HNSLQRNQRT PLPVVGTFAD GTSVRMIGEE 300
    TFRVAQQVVD EVVLVNTDEI CAAVKDIFED TRSIVEPSGA LSVAGMKKYI 350
    STVHPEIDHT KNTYVPILSG ANMNFDRLRF VSERAVLGEG KEVFMLVTLP 400
    DVPGAFKKMQ KIIHPRSVTE FSYRYNEHRH ESSSEVPKAY IYTSFSVVDR 450
    EKEIKQVMQQ LNALGFEAVD ISDNELAKSH GRYLVGGASK VPNERIISFE 500
    FPERPGALTR FLGGLSDSWN LTLFHYRNHG ADIGKVLAGI SVPPRENLTF 550
    QKFLEDLGYT YHDETDNTVY QKFLKY 576
    Length:576
    Mass (Da):63,831
    Last modified:February 1, 1995 - v2
    Checksum:i0801BCBD7EEDDC1F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti259 – 2591I → T in AAA34705. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36383 Genomic DNA. Translation: AAA34705.1.
    X01466 Genomic DNA. Translation: CAA25696.1.
    U18839 Genomic DNA. Translation: AAB64641.1.
    BK006939 Genomic DNA. Translation: DAA07746.1.
    PIRiS50589. DWBYT.
    RefSeqiNP_011009.1. NM_001178977.1.

    Genome annotation databases

    EnsemblFungiiYER086W; YER086W; YER086W.
    GeneIDi856819.
    KEGGisce:YER086W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36383 Genomic DNA. Translation: AAA34705.1 .
    X01466 Genomic DNA. Translation: CAA25696.1 .
    U18839 Genomic DNA. Translation: AAB64641.1 .
    BK006939 Genomic DNA. Translation: DAA07746.1 .
    PIRi S50589. DWBYT.
    RefSeqi NP_011009.1. NM_001178977.1.

    3D structure databases

    ProteinModelPortali P00927.
    SMRi P00927. Positions 57-388.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36830. 204 interactions.
    DIPi DIP-4029N.
    IntActi P00927. 34 interactions.
    MINTi MINT-565661.
    STRINGi 4932.YER086W.

    Proteomic databases

    MaxQBi P00927.
    PaxDbi P00927.
    PeptideAtlasi P00927.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER086W ; YER086W ; YER086W .
    GeneIDi 856819.
    KEGGi sce:YER086W.

    Organism-specific databases

    CYGDi YER086w.
    SGDi S000000888. ILV1.

    Phylogenomic databases

    eggNOGi COG1171.
    GeneTreei ENSGT00600000084626.
    HOGENOMi HOG000046975.
    KOi K01754.
    OMAi ATLEYSS.
    OrthoDBi EOG75XGVN.

    Enzyme and pathway databases

    UniPathwayi UPA00047 ; UER00054 .
    BioCyci YEAST:YER086W-MONOMER.

    Miscellaneous databases

    NextBioi 983097.
    PROi P00927.

    Gene expression databases

    Genevestigatori P00927.

    Family and domain databases

    InterProi IPR001721. ACT-like_dom.
    IPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
    IPR005787. Thr_deHydtase_biosynth.
    IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
    [Graphical view ]
    Pfami PF00291. PALP. 1 hit.
    PF00585. Thr_dehydrat_C. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53686. SSF53686. 1 hit.
    TIGRFAMsi TIGR01124. ilvA_2Cterm. 1 hit.
    PROSITEi PS51672. ACT_LIKE. 2 hits.
    PS00165. DEHYDRATASE_SER_THR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the gene for threonine deaminase (ILV1) of Saccharomyces cerevisiae."
      Kielland-Brandt M.C., Holmberg S., Petersen J.G.L., Nilsson-Tillgren T.
      Carlsberg Res. Commun. 49:567-575(1984)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Purification and properties of threonine deaminase from Saccharomyces cerevisiae."
      Ahmed S.I., Bollon A.P., Rogers S.J., Magee P.T.
      Biochimie 58:225-232(1976) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.
    5. "Regulation of isoleucine-valine biosynthesis in Saccharomyces cerevisiae."
      Holmberg S., Petersen J.G.
      Curr. Genet. 13:207-217(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME ACTIVITY, INDUCTION.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.
    9. "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
      Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
      J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTHDH_YEAST
    AccessioniPrimary (citable) accession number: P00927
    Secondary accession number(s): D3DLZ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 140 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 11900 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3