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Protein

Threonine dehydratase, mitochondrial

Gene

ILV1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 11900 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

L-threonine = 2-oxobutanoate + NH3.1 Publication

Cofactori

Enzyme regulationi

Isoleucine allosterically inhibits while valine allosterically activates this enzyme.1 Publication

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 2-oxobutanoate from L-threonine.
Proteins known to be involved in this subpathway in this organism are:
  1. Threonine dehydratase, mitochondrial (ILV1)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-oxobutanoate from L-threonine, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • L-threonine ammonia-lyase activity Source: SGD
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • isoleucine biosynthetic process Source: SGD
  • threonine catabolic process Source: SGD

Keywordsi

Molecular functionAllosteric enzyme, Lyase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis, Isoleucine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:YER086W-MONOMER
UniPathwayiUPA00047; UER00054

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine dehydratase, mitochondrial (EC:4.3.1.19)
Alternative name(s):
Threonine deaminase
Gene namesi
Name:ILV1
Ordered Locus Names:YER086W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER086W
SGDiS000000888 ILV1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000033616? – 576Threonine dehydratase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei109N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

MaxQBiP00927
PaxDbiP00927
PRIDEiP00927

Expressioni

Inductioni

In response to starvation for tryptophan and branched-chain amino acid imbalance.1 Publication

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi36830, 239 interactors
DIPiDIP-4029N
IntActiP00927, 38 interactors
MINTiP00927
STRINGi4932.YER086W

Structurei

3D structure databases

ProteinModelPortaliP00927
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini393 – 473ACT-like 1PROSITE-ProRule annotationAdd BLAST81
Domaini495 – 566ACT-like 2PROSITE-ProRule annotationAdd BLAST72

Sequence similaritiesi

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

GeneTreeiENSGT00600000084626
HOGENOMiHOG000046975
InParanoidiP00927
KOiK01754
OMAiRNHGAAY
OrthoDBiEOG092C1P3O

Family and domain databases

Gene3Di3.40.1020.10, 1 hit
InterProiView protein in InterPro
IPR001926 PLP-dep
IPR000634 Ser/Thr_deHydtase_PyrdxlP-BS
IPR001721 TD_ACT-like
IPR038110 TD_ACT-like_sf
IPR005787 Thr_deHydtase_biosynth
IPR036052 Trypto_synt_PLP_dependent
PfamiView protein in Pfam
PF00291 PALP, 1 hit
PF00585 Thr_dehydrat_C, 2 hits
SUPFAMiSSF53686 SSF53686, 1 hit
TIGRFAMsiTIGR01124 ilvA_2Cterm, 1 hit
PROSITEiView protein in PROSITE
PS51672 ACT_LIKE, 2 hits
PS00165 DEHYDRATASE_SER_THR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00927-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATLLKQPL CTVVRQGKQS KVSGLNLLRL KAHLHRQHLS PSLIKLHSEL
60 70 80 90 100
KLDELQTDNT PDYVRLVLRS SVYDVINESP ISQGVGLSSR LNTNVILKRE
110 120 130 140 150
DLLPVFSFKL RGAYNMIAKL DDSQRNQGVI ACSAGNHAQG VAFAAKHLKI
160 170 180 190 200
PATIVMPVCT PSIKYQNVSR LGSQVVLYGN DFDEAKAECA KLAEERGLTN
210 220 230 240 250
IPPFDHPYVI AGQGTVAMEI LRQVRTANKI GAVFVPVGGG GLIAGIGAYL
260 270 280 290 300
KRVAPHIKII GVETYDAATL HNSLQRNQRT PLPVVGTFAD GTSVRMIGEE
310 320 330 340 350
TFRVAQQVVD EVVLVNTDEI CAAVKDIFED TRSIVEPSGA LSVAGMKKYI
360 370 380 390 400
STVHPEIDHT KNTYVPILSG ANMNFDRLRF VSERAVLGEG KEVFMLVTLP
410 420 430 440 450
DVPGAFKKMQ KIIHPRSVTE FSYRYNEHRH ESSSEVPKAY IYTSFSVVDR
460 470 480 490 500
EKEIKQVMQQ LNALGFEAVD ISDNELAKSH GRYLVGGASK VPNERIISFE
510 520 530 540 550
FPERPGALTR FLGGLSDSWN LTLFHYRNHG ADIGKVLAGI SVPPRENLTF
560 570
QKFLEDLGYT YHDETDNTVY QKFLKY
Length:576
Mass (Da):63,831
Last modified:February 1, 1995 - v2
Checksum:i0801BCBD7EEDDC1F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti259I → T in AAA34705 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36383 Genomic DNA Translation: AAA34705.1
X01466 Genomic DNA Translation: CAA25696.1
U18839 Genomic DNA Translation: AAB64641.1
BK006939 Genomic DNA Translation: DAA07746.1
PIRiS50589 DWBYT
RefSeqiNP_011009.1, NM_001178977.1

Genome annotation databases

EnsemblFungiiYER086W; YER086W; YER086W
GeneIDi856819
KEGGisce:YER086W

Similar proteinsi

Entry informationi

Entry nameiTHDH_YEAST
AccessioniPrimary (citable) accession number: P00927
Secondary accession number(s): D3DLZ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

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