Reviewed, UniProtKB/Swiss-Prot P00922 (CAH2_SHEEP)

Names and origin

Protein names

Recommended name:
    Carbonic anhydrase 2
    EC=4.2.1.1
Alternative name(s):
    Carbonic anhydrase II
      Short name=CA-II
    Carbonate dehydratase II

Gene names

Name:

CA2

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential for bone resorption and osteoclast differentiation By similarity. Reversible hydration of carbon dioxide.
Catalytic activity	H2CO3 = CO2 + H2O.
Cofactor	Zinc.
Subunit structure	Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	One minor and three major forms were isolated chromatographically.
Sequence similarities	Belongs to the alpha-carbonic anhydrase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Lyase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carbonate dehydratase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 260	259	Carbonic anhydrase 2		PRO_0000077422
Sites
	Metal binding	94	1	Zinc; catalytic
	Metal binding	96	1	Zinc; catalytic
	Metal binding	119	1	Zinc; catalytic
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.1 Ref.2
Natural variations
	Natural variant	36	1	K → T in one of the major forms. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P00922-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 5881DD762616363D	FASTA	260	29,211
	10 20 30 40 50 60 MSHHWGYGEH NGPEHWHKDF PIADGERQSP VDIDTKAVVP DPALKPLALL YEQAASRRMV 70 80 90 100 110 120 NNGHSFNVEF DDSQDKAVLK DGPLTGTYRL VQFHFHWGSS DDQGSEHTVD RKKYAAELHL 130 140 150 160 170 180 VHWNTKYGDF GTAAQQPDGL AVVGVFLKVG DANPALQKVL DVLDSIKTKG KSADFPNFDP 190 200 210 220 230 240 SSLLKRALNY WTYPGSLTNP PLLESVTWVV LKEPTSVSSQ QMLKFRSLNF NAEGEPELLM 250 260 LANWRPAQPL KNRQVRVFPK

References

[1]	"Amino acid sequence of sheep carbonic anhydrase C." Tanis R.J., Ferrell R.E., Tashian R.E. Biochim. Biophys. Acta 371:534-548(1974) [PubMed: 4215456] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-260, ACETYLATION AT SER-2.
[2]	"Multiple molecular forms of erythrocyte carbonic anhydrase of sheep." Mallet B., Gulian J.M., Sciaky M., Laurent G., Charrel M. Biochim. Biophys. Acta 576:290-304(1979) [PubMed: 106895] [Abstract] Cited for: VARIANT THR-36.

Cross-references

Sequence databases
PIR	CRSH2. A01145.
3D structure databases
HSSP	HSSP built from PDB template 1CIM based on UniProtKB P00918.
SMR	P00922. Positions 2-260.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P00922.
Enzyme and pathway databases
BRENDA	4.2.1.1. 271.
Family and domain databases
InterPro	IPR001148. Carbonic_anhydrase_a-class_cat. IPR018338. Carbonic_anhydrase_a-class_CS. IPR018440. Carbonic_anhydrase_CA2. [Graphical view]
Gene3D	G3DSA:3.10.200.10. Euk_COanhd. 1 hit.
PANTHER	PTHR18952:SF28. Carbonic_anhydrase_CA2. 1 hit. PTHR18952. Euk_COanhd. 1 hit.
Pfam	PF00194. Carb_anhydrase. 1 hit. [Graphical view]
ProDom	PD000865. Euk_COanhd. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00162. ALPHA_CA_1. 1 hit. PS51144. ALPHA_CA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAH2_SHEEP

Accession

Primary (citable) accession number: P00922

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	September 22, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)