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P00921

- CAH2_BOVIN

UniProt

P00921 - CAH2_BOVIN

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Protein

Carbonic anhydrase 2

Gene

CA2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide.By similarity

Catalytic activityi

H2CO3 = CO2 + H2O.

Cofactori

Zinc.

Enzyme regulationi

Inhibited by acetazolamide.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei64 – 641Proton acceptorBy similarity
Active sitei67 – 671By similarity
Metal bindingi94 – 941Zinc; catalytic
Metal bindingi96 – 961Zinc; catalytic
Metal bindingi119 – 1191Zinc; catalytic
Active sitei127 – 1271By similarity

GO - Molecular functioni

  1. carbonate dehydratase activity Source: UniProtKB-EC
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. angiotensin-activated signaling pathway Source: UniProtKB
  2. one-carbon metabolic process Source: InterPro
  3. positive regulation of dipeptide transmembrane transport Source: UniProtKB
  4. regulation of anion transport Source: UniProtKB
  5. regulation of intracellular pH Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Carbonic anhydrase 2 (EC:4.2.1.1)
Alternative name(s):
Carbonate dehydratase II
Carbonic anhydrase II
Short name:
CA-II
Gene namesi
Name:CA2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 260259Carbonic anhydrase 2PRO_0000077417Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP00921.
PRIDEiP00921.

Interactioni

Subunit structurei

Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity (By similarity).By similarity

Protein-protein interaction databases

IntActiP00921. 1 interaction.

Structurei

Secondary structure

1
260
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni9 – 113
Turni13 – 153
Helixi16 – 183
Helixi21 – 244
Beta strandi25 – 273
Beta strandi31 – 333
Helixi35 – 373
Beta strandi47 – 504
Beta strandi56 – 616
Beta strandi63 – 708
Beta strandi73 – 819
Beta strandi88 – 9710
Beta strandi99 – 1024
Beta strandi106 – 1094
Beta strandi115 – 12410
Helixi125 – 1273
Helixi130 – 1334
Beta strandi139 – 15113
Helixi154 – 1563
Helixi157 – 1626
Helixi163 – 1653
Beta strandi167 – 1693
Beta strandi172 – 1743
Helixi180 – 1834
Beta strandi190 – 1956
Beta strandi206 – 2138
Beta strandi215 – 2173
Helixi219 – 2257
Beta strandi228 – 2314
Beta strandi256 – 2583

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G6VX-ray3.50A1-254[»]
1V9EX-ray1.95A/B2-260[»]
1V9IX-ray2.95C2-260[»]
ProteinModelPortaliP00921.
SMRiP00921. Positions 2-260.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00921.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni198 – 1992Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the alpha-carbonic anhydrase family.Curated

Phylogenomic databases

eggNOGiCOG3338.
HOGENOMiHOG000112637.
HOVERGENiHBG002837.
InParanoidiP00921.
KOiK18245.

Family and domain databases

Gene3Di3.10.200.10. 1 hit.
InterProiIPR018443. CA2.
IPR001148. Carbonic_anhydrase_a.
IPR023561. Carbonic_anhydrase_a-class.
IPR018338. Carbonic_anhydrase_a-class_CS.
[Graphical view]
PANTHERiPTHR18952. PTHR18952. 1 hit.
PTHR18952:SF90. PTHR18952:SF90. 1 hit.
PfamiPF00194. Carb_anhydrase. 1 hit.
[Graphical view]
SMARTiSM01057. Carb_anhydrase. 1 hit.
[Graphical view]
SUPFAMiSSF51069. SSF51069. 1 hit.
PROSITEiPS00162. ALPHA_CA_1. 1 hit.
PS51144. ALPHA_CA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00921 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSHHWGYGKH NGPEHWHKDF PIANGERQSP VDIDTKAVVQ DPALKPLALV
60 70 80 90 100
YGEATSRRMV NNGHSFNVEY DDSQDKAVLK DGPLTGTYRL VQFHFHWGSS
110 120 130 140 150
DDQGSEHTVD RKKYAAELHL VHWNTKYGDF GTAAQQPDGL AVVGVFLKVG
160 170 180 190 200
DANPALQKVL DALDSIKTKG KSTDFPNFDP GSLLPNVLDY WTYPGSLTTP
210 220 230 240 250
PLLESVTWIV LKEPISVSSQ QMLKFRTLNF NAEGEPELLM LANWRPAQPL
260
KNRQVRGFPK
Length:260
Mass (Da):29,114
Last modified:January 23, 2007 - v3
Checksum:iA89412C23FFD19A7
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti57 – 571R → Q in one of the major forms.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY240020 mRNA. Translation: AAO85140.1.
BC103260 mRNA. Translation: AAI03261.1.
PIRiA01144. CRBO2.
RefSeqiNP_848667.1. NM_178572.2.
UniGeneiBt.49731.

Genome annotation databases

GeneIDi280740.
KEGGibta:280740.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY240020 mRNA. Translation: AAO85140.1 .
BC103260 mRNA. Translation: AAI03261.1 .
PIRi A01144. CRBO2.
RefSeqi NP_848667.1. NM_178572.2.
UniGenei Bt.49731.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1G6V X-ray 3.50 A 1-254 [» ]
1V9E X-ray 1.95 A/B 2-260 [» ]
1V9I X-ray 2.95 C 2-260 [» ]
ProteinModelPortali P00921.
SMRi P00921. Positions 2-260.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P00921. 1 interaction.

Chemistry

BindingDBi P00921.
ChEMBLi CHEMBL2096971.

Proteomic databases

PaxDbi P00921.
PRIDEi P00921.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 280740.
KEGGi bta:280740.

Organism-specific databases

CTDi 760.

Phylogenomic databases

eggNOGi COG3338.
HOGENOMi HOG000112637.
HOVERGENi HBG002837.
InParanoidi P00921.
KOi K18245.

Miscellaneous databases

EvolutionaryTracei P00921.
NextBioi 20804911.

Family and domain databases

Gene3Di 3.10.200.10. 1 hit.
InterProi IPR018443. CA2.
IPR001148. Carbonic_anhydrase_a.
IPR023561. Carbonic_anhydrase_a-class.
IPR018338. Carbonic_anhydrase_a-class_CS.
[Graphical view ]
PANTHERi PTHR18952. PTHR18952. 1 hit.
PTHR18952:SF90. PTHR18952:SF90. 1 hit.
Pfami PF00194. Carb_anhydrase. 1 hit.
[Graphical view ]
SMARTi SM01057. Carb_anhydrase. 1 hit.
[Graphical view ]
SUPFAMi SSF51069. SSF51069. 1 hit.
PROSITEi PS00162. ALPHA_CA_1. 1 hit.
PS51144. ALPHA_CA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Full length cDNA of bovine carbonic anhydrase II."
    Daigle R., Castro I., Desrochers M., Charest P.-M.
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Bone marrow.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Hypothalamus.
  3. "Primary structure of bovine erythrocyte carbonic carbonic anhydrase CI. II. Complete sequence."
    Sciaky M., Limozin N., Filippi-Foveau D., Gulian J.M., Laurent-Tabusse G.
    Biochimie 58:1071-1082(1976) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-260.
    Tissue: Erythrocyte.
  4. "Genetic independence of two forms of carbonic anhydrase from bovine erythrocytes."
    Gulian J.M., Limozin N., Mallet B., di Costanzo J., Charrel M.
    Biochimie 59:293-302(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  5. "Structure of bovine carbonic anhydrase II at 1.95 A resolution."
    Saito R., Sato T., Ikai A., Tanaka N.
    Acta Crystallogr. D 60:792-795(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 2-260 IN COMPLEX WITH ZINC ION.
  6. "Antigen specificity and high affinity binding provided by one single loop of a camel single-domain antibody."
    Desmyter A., Decanniere K., Muyldermans S., Wyns L.
    J. Biol. Chem. 276:26285-26290(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.50 ANGSTROMS) OF 1-254 IN COMPLEX WITH ANTIBODY.

Entry informationi

Entry nameiCAH2_BOVIN
AccessioniPrimary (citable) accession number: P00921
Secondary accession number(s): Q3ZBJ5, Q865Y7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

One minor and two major forms were isolated chromatographically.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3