Reviewed, UniProtKB/Swiss-Prot P00917 (CAH1_HORSE)

Names and origin

Protein names

Recommended name:
    Carbonic anhydrase 1
    EC=4.2.1.1
Alternative name(s):
    Carbonic anhydrase I
      Short name=CA-I
    Carbonate dehydratase I

Gene names

Name:

CA1

Organism

Equus caballus (Horse)

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reversible hydration of carbon dioxide.
Catalytic activity	H2CO3 = CO2 + H2O.
Cofactor	Zinc.
Subcellular location	Cytoplasm.
Polymorphism	The sequence shown is that of the electrophoretically homogeneous D isozyme.
Sequence similarities	Belongs to the alpha-carbonic anhydrase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carbonate dehydratase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 261	260	Carbonic anhydrase 1		PRO_0000077408
Sites
	Metal binding	95	1	Zinc; catalytic
	Metal binding	97	1	Zinc; catalytic
	Metal binding	120	1	Zinc; catalytic
Natural variations
	Natural variant	55	1	A → D in isozyme T. Ref.1
	Natural variant	66	1	S → G in homogeneous D isozyme. Ref.1
	Natural variant	82	1	D → G in isozyme A2. Ref.1
	Natural variant	84	1	P → F in isozyme A2. Ref.1
	Natural variant	116	1	S → H in homogeneous D isozyme. Ref.1
	Natural variant	158	1	L → G in homogeneous D isozyme. Ref.1
	Natural variant	184	1	S → R in isozyme A1 and isozyme B. Ref.1
	Natural variant	213	1	C → Y in homogeneous D isozyme. Ref.1
	Natural variant	223	1	Q → R in isozyme B. Ref.1
	Natural variant	225	1	S → A in homogeneous D isozyme. Ref.1
Experimental info
	Sequence uncertainty	12	1	N or D
	Sequence uncertainty	25	1	N or D
	Sequence uncertainty	27	1	N or D
	Sequence uncertainty	28	1	N or D

Sequences

	Sequence		Length	Mass (Da)
	P00917-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: E2DD4D1BDAE62353	FASTA	261	29,025
	10 20 30 40 50 60 MAHSDWGYDS PNGPZEWVKL YPIANGNNQS PIDIKTSETK HDTSLKPFSV SYDPATAKEI 70 80 90 100 110 120 VNVGHSFQVK FEDSDNRSVL KDGPLPGSYR LVQFHFHWGS TDDYGSEHTV DGVKYSAELH 130 140 150 160 170 180 LVHWNSSKYS SFDEASSQAD GLAILGVLMK VGEANPKLQK VLDALNEVKT KGKKAPFKNF 190 200 210 220 230 240 DPSSLLPSSP DYWTYSGSLT HPPLYESVTW IVCKENISIS SQQLSQFRSL LSNVEGGKAV 250 260 PIQHNNRPPQ PLKGRTVRAF F

References

[1]

"Sequence of the low activity equine erythrocyte carbonic anhydrase and delineation of the amino acid substitutions in various polymorphic forms." Jabusch J.R., Bray R.P., Deutsch H.F. J. Biol. Chem. 255:9196-9204(1980) [PubMed: 6773961] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-261, VARIANTS ASP-55; GLY-66; GLY-82; PHE-84; HIS-116; GLY-158; ARG-184; TYR-213; ARG-223 AND ALA-225.

Cross-references

Sequence databases
PIR	CRHO1D. A01140.
3D structure databases
HSSP	HSSP built from PDB template 1HCB based on UniProtKB P00915.
SMR	P00917. Positions 5-261.
ModBase	Search...
Genome annotation databases
Ensembl	ENSECAT00000010207; ENSECAP00000007854; ENSECAG00000009144; Equus caballus. [Genome view]
Phylogenomic databases
HOVERGEN	P00917.
Enzyme and pathway databases
BRENDA	4.2.1.1. 1464.
Family and domain databases
InterPro	IPR001148. Carbonic_anhydrase_a-class_cat. IPR018338. Carbonic_anhydrase_a-class_CS. IPR018442. Carbonic_anhydrase_CA1. [Graphical view]
Gene3D	G3DSA:3.10.200.10. Euk_COanhd. 1 hit.
PANTHER	PTHR18952:SF30. Carbonic_anhydrase_CA1. 1 hit. PTHR18952. Euk_COanhd. 1 hit.
Pfam	PF00194. Carb_anhydrase. 1 hit. [Graphical view]
ProDom	PD000865. Euk_COanhd. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00162. ALPHA_CA_1. 1 hit. PS51144. ALPHA_CA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAH1_HORSE

Accession

Primary (citable) accession number: P00917

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	September 22, 2009
This is version 66 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)