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P00912 (TRPF_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-(5'-phosphoribosyl)anthranilate isomerase

Short name=PRAI
EC=5.3.1.24
Gene names
Name:TRP1
Ordered Locus Names:YDR007W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length224 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate. HAMAP-Rule MF_00135

Pathway

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5. HAMAP-Rule MF_00135

Miscellaneous

Present with 1850 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TrpF family.

Sequence caution

The sequence AAA80674.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA88067.1 differs from that shown. Reason: Erroneous termination at position 67. Translated as Ser. This mutation is only found in the substrain S288c / AB972. Resequencing of the S288c reference strain showed that S288c does not contain this mutation.

The sequence CAA88068.1 differs from that shown. Reason: Erroneous termination at position 67. Translated as Ser. This mutation is only found in the substrain S288c / AB972. Resequencing of the S288c reference strain showed that S288c does not contain this mutation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 224224N-(5'-phosphoribosyl)anthranilate isomerase HAMAP-Rule MF_00135
PRO_0000154337

Natural variations

Natural variant481I → V in strain: CLIB 556 haplotype Ha2 and CLIB 630 haplotype Ha2. Ref.4
Natural variant1721S → R in strain: CLIB 556 haplotype Ha1. Ref.4
Natural variant2121S → F in strain: CLIB 95, CLIB 382, CLIB 388, CLIB 556 haplotype Ha2, CLIB 630, K1, R12, R13, YIIc12 haplotype Ha2 and YIIc17 haplotype Ha1. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P00912 [UniParc].

Last modified December 21, 2004. Version 2.
Checksum: 03353B85F24FA09D

FASTA22424,144
        10         20         30         40         50         60 
MSVINFTGSS GPLVKVCGLQ STEAAECALD SDADLLGIIC VPNRKRTIDP VIARKISSLV 

        70         80         90        100        110        120 
KAYKNSSGTP KYLVGVFRNQ PKEDVLALVN DYGIDIVQLH GDESWQEYQE FLGLPVIKRL 

       130        140        150        160        170        180 
VFPKDCNILL SAASQKPHSF IPLFDSEAGG TGELLDWNSI SDWVGRQESP ESLHFMLAGG 

       190        200        210        220 
LTPENVGDAL RLNGVIGVDV SGGVETNGVK DSNKIANFVK NAKK 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene."
Tschumper G., Carbon J.
Gene 10:157-166(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A series of yeast shuttle vectors for expression of cDNAs and other DNA sequences."
Brunelli J.P., Pall M.L.
Yeast 9:1299-1308(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]Lieberman B.
Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Differential evolution of the Saccharomyces cerevisiae DUP240 paralogs and implication of recombination in phylogeny."
Leh-Louis V., Wirth B., Despons L., Wain-Hobson S., Potier S., Souciet J.-L.
Nucleic Acids Res. 32:2069-2078(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS VAL-48; ARG-172 AND PHE-212.
Strain: ATCC 204508 / S288c, CLIB 219, CLIB 382, CLIB 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, CLIB 95, K1, R12, R13, Sigma 1278B, YIIc12 and YIIc17.
[5]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[7]"Bent DNA at a yeast autonomously replicating sequence."
Snyder M., Buchman A.R., Davis R.W.
Nature 324:87-89(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 206-224.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V01341 Genomic DNA. Translation: CAA24634.1.
M74015 Genomic DNA. Translation: AAA72097.1.
U37458 Genomic DNA. Translation: AAA80674.1. Different initiation.
AJ585667 Genomic DNA. Translation: CAE52187.1.
AJ585668 Genomic DNA. Translation: CAE52188.1.
AJ585669 Genomic DNA. Translation: CAE52189.1.
AJ585670 Genomic DNA. Translation: CAE52190.1.
AJ585671 Genomic DNA. Translation: CAE52191.1.
AJ585672 Genomic DNA. Translation: CAE52192.1.
AJ585673 Genomic DNA. Translation: CAE52193.1.
AJ585674 Genomic DNA. Translation: CAE52194.1.
AJ585675 Genomic DNA. Translation: CAE52195.1.
AJ585676 Genomic DNA. Translation: CAE52196.1.
AJ585677 Genomic DNA. Translation: CAE52197.1.
AJ585678 Genomic DNA. Translation: CAE52198.1.
AJ585679 Genomic DNA. Translation: CAE52199.1.
AJ585680 Genomic DNA. Translation: CAE52200.1.
AJ585681 Genomic DNA. Translation: CAE52201.1.
AJ585682 Genomic DNA. Translation: CAE52202.1.
AJ585683 Genomic DNA. Translation: CAE52203.1.
AJ585684 Genomic DNA. Translation: CAE52204.1.
AJ585685 Genomic DNA. Translation: CAE52205.1.
Z48008 Genomic DNA. Translation: CAA88067.1. Sequence problems.
Z48008 Genomic DNA. Translation: CAA88068.1. Sequence problems.
M30386 Genomic DNA. Translation: AAA18406.1.
BK006938 Genomic DNA. Translation: DAA11855.1.
PIRISBYN. A01135.
RefSeqNP_010290.3. NM_001180315.3.

3D structure databases

ProteinModelPortalP00912.
SMRP00912. Positions 14-215.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32060. 35 interactions.
DIPDIP-2671N.
IntActP00912. 1 interaction.
MINTMINT-509744.

Proteomic databases

PaxDbP00912.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR007W; YDR007W; YDR007W.
GeneID851570.
KEGGsce:YDR007W.

Organism-specific databases

CYGDYDR007w.
SGDS000002414. TRP1.

Phylogenomic databases

eggNOGCOG0135.
KOK01817.
OMALAVIQCH.
OrthoDBEOG76MKM7.

Enzyme and pathway databases

BioCycYEAST:YDR007W-MONOMER.
UniPathwayUPA00035; UER00042.

Gene expression databases

GenevestigatorP00912.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00135. PRAI.
InterProIPR013785. Aldolase_TIM.
IPR001240. PRAI_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamPF00697. PRAI. 1 hit.
[Graphical view]
SUPFAMSSF51366. SSF51366. 1 hit.
ProtoNetSearch...

Other

NextBio969022.

Entry information

Entry nameTRPF_YEAST
AccessionPrimary (citable) accession number: P00912
Secondary accession number(s): D6VRZ5 expand/collapse secondary AC list , Q03448, Q12131, Q70DA4, Q70DA7, Q70DB2, Q7LHE0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 21, 2004
Last modified: February 19, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways