P00912 (TRPF_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-(5'-phosphoribosyl)anthranilate isomerase
Short name=PRAI
EC=5.3.1.24

Gene names

Name:	TRP1
Ordered Locus Names:	YDR007W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.
Miscellaneous	Present with 1850 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the TrpF family.
Sequence caution	The sequence AAA80674.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA88067.1 differs from that shown. Reason: Erroneous termination at position 67. Translated as Ser. This mutation is only found in the substrain S288c / AB972. Resequencing of the S288c reference strain showed that S288c does not contain this mutation. The sequence CAA88068.1 differs from that shown. Reason: Erroneous termination at position 67. Translated as Ser. This mutation is only found in the substrain S288c / AB972. Resequencing of the S288c reference strain showed that S288c does not contain this mutation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tryptophan biosynthetic process Inferred from direct assay PubMed 3286643. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	phosphoribosylanthranilate isomerase activity Inferred from direct assay PubMed 3286643. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	N-(5'-phosphoribosyl)anthranilate isomerase		PRO_0000154337
Natural variations
	Natural variant	48	1	I → V in strain: CLIB 556 haplotype Ha2 and CLIB 630 haplotype Ha2. Ref.4
	Natural variant	172	1	S → R in strain: CLIB 556 haplotype Ha1. Ref.4
	Natural variant	212	1	S → F in strain: CLIB 95, CLIB 382, CLIB 388, CLIB 556 haplotype Ha2, CLIB 630, K1, R12, R13, YIIc12 haplotype Ha2 and YIIc17 haplotype Ha1. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P00912 [UniParc]. Last modified December 21, 2004. Version 2. Checksum: 03353B85F24FA09D	FASTA	224	24,144
	10 20 30 40 50 60 MSVINFTGSS GPLVKVCGLQ STEAAECALD SDADLLGIIC VPNRKRTIDP VIARKISSLV 70 80 90 100 110 120 KAYKNSSGTP KYLVGVFRNQ PKEDVLALVN DYGIDIVQLH GDESWQEYQE FLGLPVIKRL 130 140 150 160 170 180 VFPKDCNILL SAASQKPHSF IPLFDSEAGG TGELLDWNSI SDWVGRQESP ESLHFMLAGG 190 200 210 220 LTPENVGDAL RLNGVIGVDV SGGVETNGVK DSNKIANFVK NAKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene." Tschumper G., Carbon J. Gene 10:157-166(1980) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A series of yeast shuttle vectors for expression of cDNAs and other DNA sequences." Brunelli J.P., Pall M.L. Yeast 9:1299-1308(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Lieberman B. Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Differential evolution of the Saccharomyces cerevisiae DUP240 paralogs and implication of recombination in phylogeny." Leh-Louis V., Wirth B., Despons L., Wain-Hobson S., Potier S., Souciet J.-L. Nucleic Acids Res. 32:2069-2078(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS VAL-48; ARG-172 AND PHE-212. Strain: ATCC 204508 / S288c, CLIB 219, CLIB 382, CLIB 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, CLIB 95, K1, R12, R13, Sigma 1278B, YIIc12 and YIIc17.
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[6]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[7]	"Bent DNA at a yeast autonomously replicating sequence." Snyder M., Buchman A.R., Davis R.W. Nature 324:87-89(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 206-224.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	V01341 Genomic DNA. Translation: CAA24634.1. M74015 Genomic DNA. Translation: AAA72097.1. U37458 Genomic DNA. Translation: AAA80674.1. Different initiation. AJ585667 Genomic DNA. Translation: CAE52187.1. AJ585668 Genomic DNA. Translation: CAE52188.1. AJ585669 Genomic DNA. Translation: CAE52189.1. AJ585670 Genomic DNA. Translation: CAE52190.1. AJ585671 Genomic DNA. Translation: CAE52191.1. AJ585672 Genomic DNA. Translation: CAE52192.1. AJ585673 Genomic DNA. Translation: CAE52193.1. AJ585674 Genomic DNA. Translation: CAE52194.1. AJ585675 Genomic DNA. Translation: CAE52195.1. AJ585676 Genomic DNA. Translation: CAE52196.1. AJ585677 Genomic DNA. Translation: CAE52197.1. AJ585678 Genomic DNA. Translation: CAE52198.1. AJ585679 Genomic DNA. Translation: CAE52199.1. AJ585680 Genomic DNA. Translation: CAE52200.1. AJ585681 Genomic DNA. Translation: CAE52201.1. AJ585682 Genomic DNA. Translation: CAE52202.1. AJ585683 Genomic DNA. Translation: CAE52203.1. AJ585684 Genomic DNA. Translation: CAE52204.1. AJ585685 Genomic DNA. Translation: CAE52205.1. Z48008 Genomic DNA. Translation: CAA88067.1. Sequence problems. Z48008 Genomic DNA. Translation: CAA88068.1. Sequence problems. M30386 Genomic DNA. Translation: AAA18406.1. BK006938 Genomic DNA. Translation: DAA11855.1.
PIR	ISBYN. A01135.
RefSeq	NP_010290.3. NM_001180315.3.
3D structure databases
ProteinModelPortal	P00912.
SMR	P00912. Positions 14-215.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2671N.
IntAct	P00912. 1 interaction.
MINT	MINT-509744.
Proteomic databases
PaxDb	P00912.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR007W; YDR007W; YDR007W.
GeneID	851570.
KEGG	sce:YDR007W. sce:YDR013W.
Organism-specific databases
CYGD	YDR007w.
SGD	S000002414. TRP1.
Phylogenomic databases
eggNOG	COG0135.
KO	K01817. K10732.
OMA	GGVETNG.
OrthoDB	EOG4GJ2ZQ.
Enzyme and pathway databases
UniPathway	UPA00035; UER00042.
Gene expression databases
Genevestigator	P00912.
GermOnline	YDR007W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR001240. PRAI. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF00697. PRAI. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
ProtoNet	Search...
Other
NextBio	969022.

Entry information

Entry name

TRPF_YEAST

Accession

Primary (citable) accession number: P00912
Secondary accession number(s): D6VRZ5 , Q03448, Q12131, Q70DA4, Q70DA7, Q70DB2, Q7LHE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	December 21, 2004
Last modified:	May 1, 2013
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program