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P00908

- TRPG_NEUCR

UniProt

P00908 - TRPG_NEUCR

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Protein
Multifunctional tryptophan biosynthesis protein
Gene
trp-1, trpg-trpc-trpf, tryp-1, NCU00200
Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.UniRule annotation

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.UniRule annotation
N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.UniRule annotation
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei104 – 1041For GATase activity By similarity
Active sitei198 – 1981For GATase activity By similarity
Active sitei200 – 2001For GATase activity By similarity

GO - Molecular functioni

  1. anthranilate synthase activity Source: UniProtKB-EC
  2. indole-3-glycerol-phosphate synthase activity Source: UniProtKB-EC
  3. phosphoribosylanthranilate isomerase activity Source: UniProtKB-EC
  4. transferase activity Source: UniProtKB-KW

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. tryptophan biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Isomerase, Lyase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional tryptophan biosynthesis protein
Including the following 3 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Short name:
IGPS
N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
Short name:
PRAI
Gene namesi
Name:trp-1
Synonyms:trpg-trpc-trpf, tryp-1
ORF Names:NCU00200
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 3, Linkage Group III

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 762762Multifunctional tryptophan biosynthesis proteinUniRule annotation
PRO_0000056861Add
BLAST

Interactioni

Subunit structurei

Tetramer of two components I and two components II.

Protein-protein interaction databases

STRINGi5141.NCU00200.1.

Structurei

3D structure databases

ProteinModelPortaliP00908.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 224200Glutamine amidotransferase type-1
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni251 – 515265Indole-3-glycerol phosphate synthaseUniRule annotation
Add
BLAST
Regioni531 – 762232N-(5'-phosphoribosyl)anthranilate isomeraseUniRule annotation
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0134.
HOGENOMiHOG000280459.
KOiK13501.
OrthoDBiEOG78WM1Q.

Family and domain databases

Gene3Di3.20.20.70. 3 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI_dom.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFiPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMiSSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00908-1 [UniParc]FASTAAdd to Basket

« Hide

MSSSSVVDHS PHDSAPSPLV PTASNLILID NYDSFTWNVY QYLVLEGAKV    50
TVFRNDQITI DELIAKNPTQ LVISPGPGHP GTDSGISRDA IRHFAGKIPI 100
FGVCMGQQCI FDVYGGDVCF AGEILHGKTS PLRHDGKGAY AGLSQDLPVT 150
RYHSLAGTHV TLPECLEVTS WIAKEDGSKG VIMGVRHKEY TIEGVQFHPE 200
SILSAEGRGM FRNFLHMQGG TWAENERLQK AAQAQAANTK SDAPTPKKSN 250
ILQKIYAHRK AAVDAQKQIP SLRPSDLQAA YNLSIAPPQI SLVDRLRNSP 300
FDVALCAEIK RASPSKGVFA LDIDAPSQAR KYALAGASVI SVLTEPEWFK 350
GSIDDLRAVR QVLNGMPNRP AVLRKEFIFD EYQILEARLA GADTVLLIVK 400
MLEYELLERL YKYSLSLGME PLVEVQNTEE MATAIKLGAK VIGVNNRNLE 450
SFEVDLGTTG RLRSMVPSDT FLCALSGINT HQDVLDCKRD GVNGILVGEA 500
IMRAPDATQF IRELCAGLTG PVPKSAAEPL LVKICGTRSA EAAAEAIKAG 550
ADLVGMIMVP GTKRCVDHET ALSISQAVHM SKKTGSTEVS SQASKSARDF 600
FNINAEIIRK RGPLLVGVFM NQPLEEVLEK QHLYDLDIVQ LHGDEPLEWA 650
NLIPVPVVRK FKPGQVGLAT RGYHAVPLLD SGAGSGTLLD LESVKKELEK 700
DEQVTVLLAG GLEPSNVVET VKSLGPLSER VIGVDVSSGV EEGGKQSLEK 750
IREFVKAAKS VR 762
Length:762
Mass (Da):82,559
Last modified:August 31, 2004 - v2
Checksum:iC058B21FF4B7C5BB
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti337 – 3371A → G1 Publication
Sequence conflicti511 – 5111I → V1 Publication
Sequence conflicti523 – 5231P → S1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01252 Genomic DNA. No translation available.
CM002238 Genomic DNA. Translation: ESA43300.1.
CM002238 Genomic DNA. Translation: ESA43301.1.
PIRiA01130. NNNC2.
RefSeqiXP_956585.1. XM_951492.1.

Genome annotation databases

EnsemblFungiiEFNCRT00000000483; EFNCRP00000000483; EFNCRG00000000483.
GeneIDi3872732.
KEGGincr:NCU00200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01252 Genomic DNA. No translation available.
CM002238 Genomic DNA. Translation: ESA43300.1 .
CM002238 Genomic DNA. Translation: ESA43301.1 .
PIRi A01130. NNNC2.
RefSeqi XP_956585.1. XM_951492.1.

3D structure databases

ProteinModelPortali P00908.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5141.NCU00200.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000000483 ; EFNCRP00000000483 ; EFNCRG00000000483 .
GeneIDi 3872732.
KEGGi ncr:NCU00200.

Phylogenomic databases

eggNOGi COG0134.
HOGENOMi HOG000280459.
KOi K13501.
OrthoDBi EOG78WM1Q.

Enzyme and pathway databases

UniPathwayi UPA00035 ; UER00040 .
UPA00035 ; UER00042 .
UPA00035 ; UER00043 .

Family and domain databases

Gene3Di 3.20.20.70. 3 hits.
3.40.50.880. 1 hit.
HAMAPi MF_00135. PRAI.
InterProi IPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI_dom.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view ]
Pfami PF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view ]
PIRSFi PIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMi SSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsi TIGR00566. trpG_papA. 1 hit.
PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of the trifunctional trp-1 gene from Neurospora crassa and its aberrant expression in Escherichia coli."
    Schechtman M.G., Yanofsky C.
    J. Mol. Appl. Genet. 2:83-99(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiTRPG_NEUCR
AccessioniPrimary (citable) accession number: P00908
Secondary accession number(s): Q7RV57, U9W354
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 31, 2004
Last modified: June 11, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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