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P00908

- TRPG_NEUCR

UniProt

P00908 - TRPG_NEUCR

Protein

Multifunctional tryptophan biosynthesis protein

Gene

trp-1

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (31 Aug 2004)
      Previous versions | rss
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    Functioni

    Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.

    Catalytic activityi

    Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
    N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
    1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei104 – 1041For GATase activityBy similarity
    Active sitei198 – 1981For GATase activityBy similarity
    Active sitei200 – 2001For GATase activityBy similarity

    GO - Molecular functioni

    1. anthranilate synthase activity Source: UniProtKB-EC
    2. indole-3-glycerol-phosphate synthase activity Source: UniProtKB-EC
    3. phosphoribosylanthranilate isomerase activity Source: UniProtKB-EC
    4. transferase activity Source: UniProtKB-KW

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB-KW
    2. tryptophan biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Decarboxylase, Isomerase, Lyase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00035; UER00040.
    UPA00035; UER00042.
    UPA00035; UER00043.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Multifunctional tryptophan biosynthesis protein
    Including the following 3 domains:
    Anthranilate synthase component 2 (EC:4.1.3.27)
    Short name:
    AS
    Alternative name(s):
    Anthranilate synthase, glutamine amidotransferase component
    Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
    Short name:
    IGPS
    N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
    Short name:
    PRAI
    Gene namesi
    Name:trp-1
    Synonyms:trpg-trpc-trpf, tryp-1
    ORF Names:NCU00200
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 3, Linkage Group III

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 762762Multifunctional tryptophan biosynthesis proteinPRO_0000056861Add
    BLAST

    Interactioni

    Subunit structurei

    Tetramer of two components I and two components II.

    Protein-protein interaction databases

    STRINGi5141.NCU00200.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP00908.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 224200Glutamine amidotransferase type-1Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni251 – 515265Indole-3-glycerol phosphate synthaseAdd
    BLAST
    Regioni531 – 762232N-(5'-phosphoribosyl)anthranilate isomeraseAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0134.
    HOGENOMiHOG000280459.
    KOiK13501.
    OrthoDBiEOG78WM1Q.

    Family and domain databases

    Gene3Di3.20.20.70. 3 hits.
    3.40.50.880. 1 hit.
    HAMAPiMF_00135. PRAI.
    InterProiIPR013785. Aldolase_TIM.
    IPR016302. Anthranilate_synth_II.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR013798. Indole-3-glycerol_P_synth.
    IPR001468. Indole-3-GlycerolPSynthase_CS.
    IPR001240. PRAI_dom.
    IPR011060. RibuloseP-bd_barrel.
    IPR006221. TrpG/PapA_dom.
    [Graphical view]
    PfamiPF00117. GATase. 1 hit.
    PF00218. IGPS. 1 hit.
    PF00697. PRAI. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001382. TrpG-trpC-trpF. 1 hit.
    SUPFAMiSSF51366. SSF51366. 3 hits.
    SSF52317. SSF52317. 1 hit.
    TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
    PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
    PS00614. IGPS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00908-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSSSVVDHS PHDSAPSPLV PTASNLILID NYDSFTWNVY QYLVLEGAKV    50
    TVFRNDQITI DELIAKNPTQ LVISPGPGHP GTDSGISRDA IRHFAGKIPI 100
    FGVCMGQQCI FDVYGGDVCF AGEILHGKTS PLRHDGKGAY AGLSQDLPVT 150
    RYHSLAGTHV TLPECLEVTS WIAKEDGSKG VIMGVRHKEY TIEGVQFHPE 200
    SILSAEGRGM FRNFLHMQGG TWAENERLQK AAQAQAANTK SDAPTPKKSN 250
    ILQKIYAHRK AAVDAQKQIP SLRPSDLQAA YNLSIAPPQI SLVDRLRNSP 300
    FDVALCAEIK RASPSKGVFA LDIDAPSQAR KYALAGASVI SVLTEPEWFK 350
    GSIDDLRAVR QVLNGMPNRP AVLRKEFIFD EYQILEARLA GADTVLLIVK 400
    MLEYELLERL YKYSLSLGME PLVEVQNTEE MATAIKLGAK VIGVNNRNLE 450
    SFEVDLGTTG RLRSMVPSDT FLCALSGINT HQDVLDCKRD GVNGILVGEA 500
    IMRAPDATQF IRELCAGLTG PVPKSAAEPL LVKICGTRSA EAAAEAIKAG 550
    ADLVGMIMVP GTKRCVDHET ALSISQAVHM SKKTGSTEVS SQASKSARDF 600
    FNINAEIIRK RGPLLVGVFM NQPLEEVLEK QHLYDLDIVQ LHGDEPLEWA 650
    NLIPVPVVRK FKPGQVGLAT RGYHAVPLLD SGAGSGTLLD LESVKKELEK 700
    DEQVTVLLAG GLEPSNVVET VKSLGPLSER VIGVDVSSGV EEGGKQSLEK 750
    IREFVKAAKS VR 762
    Length:762
    Mass (Da):82,559
    Last modified:August 31, 2004 - v2
    Checksum:iC058B21FF4B7C5BB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti337 – 3371A → G(PubMed:6221060)Curated
    Sequence conflicti511 – 5111I → V(PubMed:6221060)Curated
    Sequence conflicti523 – 5231P → S(PubMed:6221060)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01252 Genomic DNA. No translation available.
    CM002238 Genomic DNA. Translation: ESA43300.1.
    CM002238 Genomic DNA. Translation: ESA43301.1.
    PIRiA01130. NNNC2.
    RefSeqiXP_956585.1. XM_951492.1.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000000483; EFNCRP00000000483; EFNCRG00000000483.
    GeneIDi3872732.
    KEGGincr:NCU00200.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01252 Genomic DNA. No translation available.
    CM002238 Genomic DNA. Translation: ESA43300.1 .
    CM002238 Genomic DNA. Translation: ESA43301.1 .
    PIRi A01130. NNNC2.
    RefSeqi XP_956585.1. XM_951492.1.

    3D structure databases

    ProteinModelPortali P00908.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU00200.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000000483 ; EFNCRP00000000483 ; EFNCRG00000000483 .
    GeneIDi 3872732.
    KEGGi ncr:NCU00200.

    Phylogenomic databases

    eggNOGi COG0134.
    HOGENOMi HOG000280459.
    KOi K13501.
    OrthoDBi EOG78WM1Q.

    Enzyme and pathway databases

    UniPathwayi UPA00035 ; UER00040 .
    UPA00035 ; UER00042 .
    UPA00035 ; UER00043 .

    Family and domain databases

    Gene3Di 3.20.20.70. 3 hits.
    3.40.50.880. 1 hit.
    HAMAPi MF_00135. PRAI.
    InterProi IPR013785. Aldolase_TIM.
    IPR016302. Anthranilate_synth_II.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR013798. Indole-3-glycerol_P_synth.
    IPR001468. Indole-3-GlycerolPSynthase_CS.
    IPR001240. PRAI_dom.
    IPR011060. RibuloseP-bd_barrel.
    IPR006221. TrpG/PapA_dom.
    [Graphical view ]
    Pfami PF00117. GATase. 1 hit.
    PF00218. IGPS. 1 hit.
    PF00697. PRAI. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001382. TrpG-trpC-trpF. 1 hit.
    SUPFAMi SSF51366. SSF51366. 3 hits.
    SSF52317. SSF52317. 1 hit.
    TIGRFAMsi TIGR00566. trpG_papA. 1 hit.
    PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
    PS00614. IGPS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of the trifunctional trp-1 gene from Neurospora crassa and its aberrant expression in Escherichia coli."
      Schechtman M.G., Yanofsky C.
      J. Mol. Appl. Genet. 2:83-99(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

    Entry informationi

    Entry nameiTRPG_NEUCR
    AccessioniPrimary (citable) accession number: P00908
    Secondary accession number(s): Q7RV57, U9W354
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: August 31, 2004
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3