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Protein

Multifunctional tryptophan biosynthesis protein

Gene

trp-1

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.

Pathway: L-tryptophan biosynthesis

This protein is involved in step 1, 3 and 4 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Multifunctional tryptophan biosynthesis protein (trp-1)
  2. no protein annotated in this organism
  3. Multifunctional tryptophan biosynthesis protein (trp-1)
  4. Multifunctional tryptophan biosynthesis protein (trp-1)
  5. Tryptophan synthase (trp-3)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei104 – 1041For GATase activityBy similarity
Active sitei198 – 1981For GATase activityBy similarity
Active sitei200 – 2001For GATase activityBy similarity

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Decarboxylase, Isomerase, Lyase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Protein family/group databases

MEROPSiC26.959.

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional tryptophan biosynthesis protein
Including the following 3 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Short name:
IGPS
N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
Short name:
PRAI
Gene namesi
Name:trp-1
Synonyms:trpg-trpc-trpf, tryp-1
ORF Names:NCU00200
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805 Componenti: Chromosome 3, Linkage Group III

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 762762Multifunctional tryptophan biosynthesis proteinPRO_0000056861Add
BLAST

Interactioni

Subunit structurei

Tetramer of two components I and two components II.

Protein-protein interaction databases

STRINGi5141.EFNCRP00000000483.

Structurei

3D structure databases

ProteinModelPortaliP00908.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 224200Glutamine amidotransferase type-1Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni251 – 515265Indole-3-glycerol phosphate synthaseAdd
BLAST
Regioni531 – 762232N-(5'-phosphoribosyl)anthranilate isomeraseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0134.
HOGENOMiHOG000280459.
InParanoidiP00908.
KOiK13501.
OrthoDBiEOG78WM1Q.

Family and domain databases

Gene3Di3.20.20.70. 3 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFiPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMiSSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00908-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSSVVDHS PHDSAPSPLV PTASNLILID NYDSFTWNVY QYLVLEGAKV
60 70 80 90 100
TVFRNDQITI DELIAKNPTQ LVISPGPGHP GTDSGISRDA IRHFAGKIPI
110 120 130 140 150
FGVCMGQQCI FDVYGGDVCF AGEILHGKTS PLRHDGKGAY AGLSQDLPVT
160 170 180 190 200
RYHSLAGTHV TLPECLEVTS WIAKEDGSKG VIMGVRHKEY TIEGVQFHPE
210 220 230 240 250
SILSAEGRGM FRNFLHMQGG TWAENERLQK AAQAQAANTK SDAPTPKKSN
260 270 280 290 300
ILQKIYAHRK AAVDAQKQIP SLRPSDLQAA YNLSIAPPQI SLVDRLRNSP
310 320 330 340 350
FDVALCAEIK RASPSKGVFA LDIDAPSQAR KYALAGASVI SVLTEPEWFK
360 370 380 390 400
GSIDDLRAVR QVLNGMPNRP AVLRKEFIFD EYQILEARLA GADTVLLIVK
410 420 430 440 450
MLEYELLERL YKYSLSLGME PLVEVQNTEE MATAIKLGAK VIGVNNRNLE
460 470 480 490 500
SFEVDLGTTG RLRSMVPSDT FLCALSGINT HQDVLDCKRD GVNGILVGEA
510 520 530 540 550
IMRAPDATQF IRELCAGLTG PVPKSAAEPL LVKICGTRSA EAAAEAIKAG
560 570 580 590 600
ADLVGMIMVP GTKRCVDHET ALSISQAVHM SKKTGSTEVS SQASKSARDF
610 620 630 640 650
FNINAEIIRK RGPLLVGVFM NQPLEEVLEK QHLYDLDIVQ LHGDEPLEWA
660 670 680 690 700
NLIPVPVVRK FKPGQVGLAT RGYHAVPLLD SGAGSGTLLD LESVKKELEK
710 720 730 740 750
DEQVTVLLAG GLEPSNVVET VKSLGPLSER VIGVDVSSGV EEGGKQSLEK
760
IREFVKAAKS VR
Length:762
Mass (Da):82,559
Last modified:August 31, 2004 - v2
Checksum:iC058B21FF4B7C5BB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti337 – 3371A → G (PubMed:6221060).Curated
Sequence conflicti511 – 5111I → V (PubMed:6221060).Curated
Sequence conflicti523 – 5231P → S (PubMed:6221060).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01252 Genomic DNA. No translation available.
CM002238 Genomic DNA. Translation: ESA43300.1.
CM002238 Genomic DNA. Translation: ESA43301.1.
PIRiA01130. NNNC2.

Genome annotation databases

EnsemblFungiiEFNCRT00000000483; EFNCRP00000000483; EFNCRG00000000483.
KEGGincr:NCU00200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01252 Genomic DNA. No translation available.
CM002238 Genomic DNA. Translation: ESA43300.1.
CM002238 Genomic DNA. Translation: ESA43301.1.
PIRiA01130. NNNC2.

3D structure databases

ProteinModelPortaliP00908.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5141.EFNCRP00000000483.

Protein family/group databases

MEROPSiC26.959.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEFNCRT00000000483; EFNCRP00000000483; EFNCRG00000000483.
KEGGincr:NCU00200.

Phylogenomic databases

eggNOGiCOG0134.
HOGENOMiHOG000280459.
InParanoidiP00908.
KOiK13501.
OrthoDBiEOG78WM1Q.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Family and domain databases

Gene3Di3.20.20.70. 3 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFiPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMiSSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structure of the trifunctional trp-1 gene from Neurospora crassa and its aberrant expression in Escherichia coli."
    Schechtman M.G., Yanofsky C.
    J. Mol. Appl. Genet. 2:83-99(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiTRPG_NEUCR
AccessioniPrimary (citable) accession number: P00908
Secondary accession number(s): Q7RV57, U9W354
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 31, 2004
Last modified: June 24, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.