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Protein

Multifunctional tryptophan biosynthesis protein

Gene

trp-1

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1, 3 and 4 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Multifunctional tryptophan biosynthesis protein (trp-1)
  2. no protein annotated in this organism
  3. Multifunctional tryptophan biosynthesis protein (trp-1)
  4. Multifunctional tryptophan biosynthesis protein (trp-1)
  5. Tryptophan synthase (trp-3)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei104Nucleophile; for GATase activityBy similarity1
Binding sitei108GlutamineBy similarity1
Active sitei198For GATase activityBy similarity1
Active sitei200For GATase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Isomerase, Lyase, Multifunctional enzyme
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040
UPA00035; UER00042
UPA00035; UER00043

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional tryptophan biosynthesis protein
Including the following 3 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Short name:
IGPS
N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
Short name:
PRAI
Gene namesi
Name:trp-1
Synonyms:trpg-trpc-trpf, tryp-1
ORF Names:NCU00200
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 3, Linkage Group III

Organism-specific databases

EuPathDBiFungiDB:NCU00200

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000568611 – 762Multifunctional tryptophan biosynthesis proteinAdd BLAST762

Proteomic databases

PRIDEiP00908

Interactioni

Subunit structurei

Tetramer of two components I and two components II.

Structurei

3D structure databases

ProteinModelPortaliP00908
SMRiP00908
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 224Glutamine amidotransferase type-1Add BLAST200

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 78Glutamine bindingBy similarity3
Regioni154 – 155Glutamine bindingBy similarity2
Regioni251 – 515Indole-3-glycerol phosphate synthaseAdd BLAST265
Regioni531 – 762N-(5'-phosphoribosyl)anthranilate isomeraseAdd BLAST232

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000280459
InParanoidiP00908
KOiK13501
OrthoDBiEOG092C1PKC

Family and domain databases

CDDicd00331 IGPS, 1 hit
cd00405 PRAI, 1 hit
Gene3Di3.20.20.70, 3 hits
3.40.50.880, 1 hit
HAMAPiMF_00135 PRAI, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR016302 Anthranilate_synth_II
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR013798 Indole-3-glycerol_P_synth
IPR001468 Indole-3-GlycerolPSynthase_CS
IPR001240 PRAI
IPR011060 RibuloseP-bd_barrel
IPR006221 TrpG/PapA_dom
PfamiView protein in Pfam
PF00117 GATase, 1 hit
PF00218 IGPS, 1 hit
PF00697 PRAI, 1 hit
PIRSFiPIRSF001382 TrpG-trpC-trpF, 1 hit
SUPFAMiSSF51366 SSF51366, 3 hits
SSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR00566 trpG_papA, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit
PS00614 IGPS, 1 hit

Sequencei

Sequence statusi: Complete.

P00908-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSSVVDHS PHDSAPSPLV PTASNLILID NYDSFTWNVY QYLVLEGAKV
60 70 80 90 100
TVFRNDQITI DELIAKNPTQ LVISPGPGHP GTDSGISRDA IRHFAGKIPI
110 120 130 140 150
FGVCMGQQCI FDVYGGDVCF AGEILHGKTS PLRHDGKGAY AGLSQDLPVT
160 170 180 190 200
RYHSLAGTHV TLPECLEVTS WIAKEDGSKG VIMGVRHKEY TIEGVQFHPE
210 220 230 240 250
SILSAEGRGM FRNFLHMQGG TWAENERLQK AAQAQAANTK SDAPTPKKSN
260 270 280 290 300
ILQKIYAHRK AAVDAQKQIP SLRPSDLQAA YNLSIAPPQI SLVDRLRNSP
310 320 330 340 350
FDVALCAEIK RASPSKGVFA LDIDAPSQAR KYALAGASVI SVLTEPEWFK
360 370 380 390 400
GSIDDLRAVR QVLNGMPNRP AVLRKEFIFD EYQILEARLA GADTVLLIVK
410 420 430 440 450
MLEYELLERL YKYSLSLGME PLVEVQNTEE MATAIKLGAK VIGVNNRNLE
460 470 480 490 500
SFEVDLGTTG RLRSMVPSDT FLCALSGINT HQDVLDCKRD GVNGILVGEA
510 520 530 540 550
IMRAPDATQF IRELCAGLTG PVPKSAAEPL LVKICGTRSA EAAAEAIKAG
560 570 580 590 600
ADLVGMIMVP GTKRCVDHET ALSISQAVHM SKKTGSTEVS SQASKSARDF
610 620 630 640 650
FNINAEIIRK RGPLLVGVFM NQPLEEVLEK QHLYDLDIVQ LHGDEPLEWA
660 670 680 690 700
NLIPVPVVRK FKPGQVGLAT RGYHAVPLLD SGAGSGTLLD LESVKKELEK
710 720 730 740 750
DEQVTVLLAG GLEPSNVVET VKSLGPLSER VIGVDVSSGV EEGGKQSLEK
760
IREFVKAAKS VR
Length:762
Mass (Da):82,559
Last modified:August 31, 2004 - v2
Checksum:iC058B21FF4B7C5BB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti337A → G (PubMed:6221060).Curated1
Sequence conflicti511I → V (PubMed:6221060).Curated1
Sequence conflicti523P → S (PubMed:6221060).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01252 Genomic DNA No translation available.
CM002238 Genomic DNA Translation: ESA43300.1
CM002238 Genomic DNA Translation: ESA43301.1
PIRiA01130 NNNC2
RefSeqiXP_011393782.1, XM_011395480.1
XP_011393783.1, XM_011395481.1

Genome annotation databases

EnsemblFungiiESA43300; ESA43300; NCU00200
ESA43301; ESA43301; NCU00200
GeneIDi3872732
KEGGincr:NCU00200

Similar proteinsi

Entry informationi

Entry nameiTRPG_NEUCR
AccessioniPrimary (citable) accession number: P00908
Secondary accession number(s): Q7RV57, U9W354
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 31, 2004
Last modified: May 23, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health