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Protein

Anthranilate synthase component II

Gene

trpG-TRPD

Organism
Shigella dysenteriae
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate = anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei84 – 841For GATase activityPROSITE-ProRule annotation
Active sitei170 – 1701For GATase activityPROSITE-ProRule annotation
Active sitei172 – 1721For GATase activityPROSITE-ProRule annotation

GO - Molecular functioni

  1. anthranilate phosphoribosyltransferase activity Source: UniProtKB-EC
  2. anthranilate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. tryptophan biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
UPA00035; UER00041.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component II (EC:4.1.3.27)
Including the following 2 domains:
Glutamine amidotransferase
Anthranilate phosphoribosyltransferase (EC:2.4.2.18)
Gene namesi
Name:trpG-TRPD
OrganismiShigella dysenteriae
Taxonomic identifieri622 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – ›201›200Anthranilate synthase component IIPRO_0000056899Add
BLAST

Interactioni

Subunit structurei

Tetramer of two components I and two components II.

Structurei

3D structure databases

ProteinModelPortaliP00906.
SMRiP00906. Positions 2-193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 196194Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00906-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADILLLDNI DSFTYNLADQ LRSNGHNVVI YRNHIPAQTL IERLATMSNP
60 70 80 90 100
VLMLSPGPGV PSEAGCMPEL LTRLSGKLPI IGICLGHQAI VEAYGGYVGQ
110 120 130 140 150
AGEILHGKAS SIEHDGQAMF AGLTNPLPVA RYHSLVGSNI PAGLTINAHF
160 170 180 190 200
NGMVMAVRHD ADRICGFQFH PESILTTQGA RLLEQTLAWA QRKLEPTNTL

Q
Length:201
Mass (Da):21,700
Last modified:January 23, 2007 - v2
Checksum:i943E3DBE4F0E59AF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei201 – 2011

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01787 Genomic DNA. Translation: AAA57307.1.
PIRiB92860. NNEB2D.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01787 Genomic DNA. Translation: AAA57307.1.
PIRiB92860. NNEB2D.

3D structure databases

ProteinModelPortaliP00906.
SMRiP00906. Positions 2-193.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
UPA00035; UER00041.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequences of the trpG regions of Escherichia coli, Shigella dysenteriae, Salmonella typhimurium and Serratia marcescens."
    Nichols B.P., Miozzari G.F., van Cleemput M., Bennett G.N., Yanofsky C.
    J. Mol. Biol. 142:503-517(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiTRPG_SHIDY
AccessioniPrimary (citable) accession number: P00906
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
In E.coli and certain other bacteria, component II is larger and its C-terminal two thirds has anthranilate phosphoribosyltransferase activity.

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.