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Protein

Bifunctional protein TrpGD

Gene

trpGD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia. In addition to synthesizing anthranilate, it also catalyzes the second step of the pathway, the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate.3 Publications

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate = anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate.

Enzyme regulationi

Cooperatively feedback inhibited by tryptophan.3 Publications

Kineticsi

  1. KM=1.2 µM for chorismate (at pH 7.5)3 Publications
  2. KM=5 µM for chorismate (at 25 degrees Celsius and at pH 7.8)3 Publications
  3. KM=360 µM for glutamine (at pH 7.5)3 Publications
  1. Vmax=0.53 nmol/min/mg enzyme (at 25 degrees Celsius and at pH 7.8)3 Publications

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1 and 2 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Bifunctional protein TrpGD (trpGD), Anthranilate synthase component 1 (trpE)
  2. Bifunctional protein TrpGD (trpGD)
  3. Tryptophan biosynthesis protein TrpCF (trpC)
  4. Tryptophan biosynthesis protein TrpCF (trpC)
  5. Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei84Nucleophile; for GATase activityBy similarity1
Binding sitei88GlutamineBy similarity1
Active sitei170For GATase activityBy similarity1
Active sitei172For GATase activityBy similarity1

GO - Molecular functioni

  • anthranilate phosphoribosyltransferase activity Source: EcoCyc
  • anthranilate synthase activity Source: EcoCyc

GO - Biological processi

  • glutamine metabolic process Source: UniProtKB-KW
  • tryptophan biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionAllosteric enzyme, Glycosyltransferase, Lyase, Multifunctional enzyme, Transferase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:ANTHRANSYNCOMPII-MONOMER
MetaCyc:ANTHRANSYNCOMPII-MONOMER
UniPathwayiUPA00035; UER00040
UPA00035; UER00041

Protein family/group databases

MEROPSiC26.960

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein TrpGD
Including the following 2 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Short name:
ASII
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Anthranilate phosphoribosyltransferase (EC:2.4.2.18)
Gene namesi
Name:trpGD
Synonyms:trpD
Ordered Locus Names:b1263, JW1255
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11027 trpD

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000568772 – 531Bifunctional protein TrpGDAdd BLAST530

Proteomic databases

PaxDbiP00904
PRIDEiP00904

2D gel databases

SWISS-2DPAGEP00904

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).By similarity

Protein-protein interaction databases

BioGridi4260123, 27 interactors
STRINGi316385.ECDH10B_1378

Structurei

3D structure databases

ProteinModelPortaliP00904
SMRiP00904
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 196Glutamine amidotransferase type-1Add BLAST194

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni57 – 59Glutamine bindingBy similarity3
Regioni134 – 135Glutamine bindingBy similarity2
Regioni202 – 531Anthranilate phosphoribosyltransferaseAdd BLAST330

Sequence similaritiesi

In the C-terminal section; belongs to the anthranilate phosphoribosyltransferase family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4108EFP Bacteria
COG0512 LUCA
COG0547 LUCA
HOGENOMiHOG000230451
InParanoidiP00904
KOiK13497
OMAiKCFNTLQ
PhylomeDBiP00904

Family and domain databases

Gene3Di3.40.1030.10, 2 hits
3.40.50.880, 1 hit
HAMAPiMF_00211 TrpD, 1 hit
InterProiView protein in InterPro
IPR005940 Anthranilate_Pribosyl_Tfrase
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR000312 Glycosyl_Trfase_fam3
IPR017459 Glycosyl_Trfase_fam3_N_dom
IPR036320 Glycosyl_Trfase_fam3_N_dom)sf
IPR035902 Nuc_phospho_transferase
IPR006221 TrpG/PapA_dom
PfamiView protein in Pfam
PF00117 GATase, 1 hit
PF02885 Glycos_trans_3N, 1 hit
PF00591 Glycos_transf_3, 1 hit
SUPFAMiSSF47648 SSF47648, 1 hit
SSF52317 SSF52317, 1 hit
SSF52418 SSF52418, 1 hit
TIGRFAMsiTIGR01245 trpD, 1 hit
TIGR00566 trpG_papA, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00904-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADILLLDNI DSFTYNLADQ LRSNGHNVVI YRNHIPAQTL IERLATMSNP
60 70 80 90 100
VLMLSPGPGV PSEAGCMPEL LTRLRGKLPI IGICLGHQAI VEAYGGYVGQ
110 120 130 140 150
AGEILHGKAS SIEHDGQAMF AGLTNPLPVA RYHSLVGSNI PAGLTINAHF
160 170 180 190 200
NGMVMAVRHD ADRVCGFQFH PESILTTQGA RLLEQTLAWA QQKLEPANTL
210 220 230 240 250
QPILEKLYQA QTLSQQESHQ LFSAVVRGEL KPEQLAAALV SMKIRGEHPN
260 270 280 290 300
EIAGAATALL ENAAPFPRPD YLFADIVGTG GDGSNSINIS TASAFVAAAC
310 320 330 340 350
GLKVAKHGNR SVSSKSGSSD LLAAFGINLD MNADKSRQAL DELGVCFLFA
360 370 380 390 400
PKYHTGFRHA MPVRQQLKTR TLFNVLGPLI NPAHPPLALI GVYSPELVLP
410 420 430 440 450
IAETLRVLGY QRAAVVHSGG MDEVSLHAPT IVAELHDGEI KSYQLTAEDF
460 470 480 490 500
GLTPYHQEQL AGGTPEENRD ILTRLLQGKG DAAHEAAVAA NVAMLMRLHG
510 520 530
HEDLQANAQT VLEVLRSGSA YDRVTALAAR G
Length:531
Mass (Da):56,870
Last modified:January 23, 2007 - v3
Checksum:i10B02E455194F072
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61P → A in CAA23665 (PubMed:6283099).Curated1
Sequence conflicti192Q → H in CAA23672 (PubMed:7038627).Curated1
Sequence conflicti192Q → H in CAA23665 (PubMed:6283099).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00372 Genomic DNA Translation: CAA23672.1
V00367 Genomic DNA Translation: CAA23665.1
J01714 Genomic DNA Translation: AAA57298.1
U00096 Genomic DNA Translation: AAC74345.1
AP009048 Genomic DNA Translation: BAA14798.1
PIRiB64874 NNEC2
RefSeqiNP_415779.1, NC_000913.3
WP_000763511.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74345; AAC74345; b1263
BAA14798; BAA14798; BAA14798
GeneIDi945109
KEGGiecj:JW1255
eco:b1263
PATRICifig|1411691.4.peg.1020

Similar proteinsi

Entry informationi

Entry nameiTRPGD_ECOLI
AccessioniPrimary (citable) accession number: P00904
Secondary accession number(s): P76833, P76835, P78302
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 163 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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