P00901 (TRPG_PSEPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anthranilate synthase component II
EC=4.1.3.27

Alternative name(s):
Glutamine amido-transferase

Gene names

Name:

trpG

Organism

Pseudomonas putida (Arthrobacter siderocapsulatus)

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Contains 2 chains with different activities: component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Domain	Glutamine amidotransferase
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	Anthranilate synthase component II		PRO_0000056896
Regions
	Domain	2 – 198	197	Glutamine amidotransferase type-1
Sites
	Active site	80	1
	Active site	174	1	By similarity
	Active site	176	1	By similarity
Experimental info
	Sequence conflict	5	1	Missing AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P00901 [UniParc]. Last modified February 1, 1991. Version 2. Checksum: 6AA7BDC12ED02FB3	FASTA	198	21,824
	10 20 30 40 50 60 MLLMMIDNYD SFTYNVVQYL GELGAEVKVI RNDEMTIAQI EALNPERIVV SPGPCTPSEA 70 80 90 100 110 120 GVSIEAILHF AGKLPILGVC LGHQSIGQAF GGDVVRARQV MHGKTSPVHH RDLGVFTGLN 130 140 150 160 170 180 NPLTVTRYHS LVVKRETLPD CLEVTAWTAH EDGSVDEIMG LRHKTLNIEG VQFHPESILT 190 EQGHELFANF LKQTGGRR

References

[1]	"Evolutionary differences in chromosomal locations of four early genes of the tryptophan pathway in fluorescent pseudomonads: DNA sequences and characterization of Pseudomonas putida trpE and trpGDC." Essar D.W., Eberly L., Crawford I.P. J. Bacteriol. 172:867-883(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 23287 / C1S.
[2]	"Anthranilate synthetase component II from Pseudomonas putida. Covalent structure and identification of the cysteine residue involved in catalysis." Kawamura M., Keim P.S., Goto Y., Zalkin H., Heinrikson R.L. J. Biol. Chem. 253:4659-4668(1978) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M33799 Genomic DNA. Translation: AAA80553.1.
PIR	NNPS2P. A01122.
3D structure databases
ProteinModelPortal	P00901.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00035; UER00040.
Family and domain databases
InterPro	IPR017926. GATASE_1. IPR006221. TrpG/PapA. [Graphical view]
Pfam	PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00566. trpG_papA. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPG_PSEPU

Accession

Primary (citable) accession number: P00901

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	February 1, 1991
Last modified:	April 3, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program