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P00900

- TRPG_SERMA

UniProt

P00900 - TRPG_SERMA

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Protein
Anthranilate synthase component 2
Gene
trpG
Organism
Serratia marcescens
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia Inferred.1 Publication

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei84 – 841For GATase activity Inferred
Binding sitei84 – 841Glutamine
Binding sitei88 – 881Glutamine
Active sitei170 – 1701For GATase activity By similarity
Active sitei172 – 1721For GATase activity By similarity

GO - Molecular functioni

  1. anthranilate synthase activity Source: UniProtKB-EC
  2. protein binding Source: IntAct

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. tryptophan biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Short name:
ASII
Alternative name(s):
Anthranilate synthase, GATase component
Anthranilate synthase, glutamine amidotransferase component
Gene namesi
Name:trpG
OrganismiSerratia marcescens
Taxonomic identifieri615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 193192Anthranilate synthase component 2
PRO_0000056898Add
BLAST

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
trpEP008972EBI-1031352,EBI-1031345

Protein-protein interaction databases

IntActiP00900. 1 interaction.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86
Helixi14 – 2310
Beta strandi27 – 326
Helixi37 – 4610
Beta strandi48 – 547
Helixi61 – 633
Helixi67 – 748
Turni75 – 773
Beta strandi80 – 834
Helixi85 – 939
Beta strandi97 – 11418
Helixi118 – 1203
Beta strandi125 – 13915
Beta strandi144 – 1507
Beta strandi153 – 1597
Turni160 – 1634
Beta strandi164 – 1696
Helixi179 – 19113

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1I7QX-ray1.95B/D1-193[»]
1I7SX-ray2.40B/D1-193[»]
ProteinModelPortaliP00900.
SMRiP00900. Positions 2-193.

Miscellaneous databases

EvolutionaryTraceiP00900.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 193191Glutamine amidotransferase type-1
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni57 – 593Glutamine binding
Regioni134 – 1352Glutamine binding

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00900-1 [UniParc]FASTAAdd to Basket

« Hide

MADILLLDNV DSFTYNLVDQ LRASGHQVVI YRNQIGAEVI IERLQHMEQP    50
VLMLSPGPGT PSEAGCMPEL LQRLRGQLPI IGICLGHQAI VEAYGGQVGQ 100
AGEILHGKAS AIAHDGEGMF AGMANPLPVA RYHSLVGSNI PADLTVNARS 150
GEMVMAVRDD RRRVCGFQFH PESILTTHGA RLLEQTLAWA LAK 193
Length:193
Mass (Da):20,868
Last modified:January 23, 2007 - v2
Checksum:i8F53EBFEC7F4524A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361G → A AA sequence 1 Publication
Sequence conflicti38 – 381E → Q AA sequence 1 Publication
Sequence conflicti38 – 381E → Q AA sequence 1 Publication
Sequence conflicti45 – 451Q → E AA sequence 1 Publication
Sequence conflicti46 – 461H → Q AA sequence 1 Publication
Sequence conflicti48 – 481E → T AA sequence 1 Publication
Sequence conflicti60 – 623TPS → ASV AA sequence 1 Publication
Sequence conflicti77 – 771Q → R AA sequence 1 Publication
Sequence conflicti150 – 1501S → F AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY027546 Genomic DNA. Translation: AAK37409.1.
PIRiD92860. NNSE2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY027546 Genomic DNA. Translation: AAK37409.1 .
PIRi D92860. NNSE2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1I7Q X-ray 1.95 B/D 1-193 [» ]
1I7S X-ray 2.40 B/D 1-193 [» ]
ProteinModelPortali P00900.
SMRi P00900. Positions 2-193.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P00900. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00035 ; UER00040 .

Miscellaneous databases

EvolutionaryTracei P00900.

Family and domain databases

Gene3Di 3.40.50.880. 1 hit.
InterProi IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR006221. TrpG/PapA_dom.
[Graphical view ]
Pfami PF00117. GATase. 1 hit.
[Graphical view ]
SUPFAMi SSF52317. SSF52317. 1 hit.
TIGRFAMsi TIGR00566. trpG_papA. 1 hit.
PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequences of the trpG regions of Escherichia coli, Shigella dysenteriae, Salmonella typhimurium and Serratia marcescens."
    Nichols B.P., Miozzari G.F., van Cleemput M., Bennett G.N., Yanofsky C.
    J. Mol. Biol. 142:503-517(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Primary structure of Serratia marcescens anthranilate synthase component II."
    Tso J.Y., Hermodson M.A., Zalkin H.
    J. Biol. Chem. 255:1451-1457(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-193.
  3. "Separation of anthranilate synthetase components I and II of Escherichia coli, Salmonella typhimurium, and Serratia marcescens and determination of their amino-terminal sequences by automatic Edman degradation."
    Li S.-L., Hanlon J., Yanofsky C.
    Biochemistry 13:1736-1744(1974) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-62.
  4. "The structures of anthranilate synthase of Serratia marcescens crystallized in the presence of (i) its substrates, chorismate and glutamine, and a product, glutamate, and (ii) its end-product inhibitor, L-tryptophan."
    Spraggon G., Kim C., Nguyen-Huu X., Yee M.-C., Yanofsky C., Mills S.E.
    Proc. Natl. Acad. Sci. U.S.A. 98:6021-6026(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH SUBSTRATE, FUNCTION, ACTIVE SITE, SUBUNIT.

Entry informationi

Entry nameiTRPG_SERMA
AccessioniPrimary (citable) accession number: P00900
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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