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P00897

- TRPE_SERMA

UniProt

P00897 - TRPE_SERMA

Protein

Anthranilate synthase component 1

Gene

trpE

Organism
Serratia marcescens
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (14 Aug 2001)
      Previous versions | rss
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    Functioni

    Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia Probable.1 Publication

    Catalytic activityi

    Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

    Cofactori

    Binds 1 magnesium ion per subunit.1 Publication

    Enzyme regulationi

    Feedback inhibited by tryptophan.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei39 – 391Tryptophan
    Metal bindingi360 – 3601Magnesium
    Binding sitei448 – 4481Substrate
    Binding sitei468 – 4681Substrate
    Binding sitei484 – 4841Substrate; via amide nitrogen
    Metal bindingi497 – 4971Magnesium

    GO - Molecular functioni

    1. anthranilate synthase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. tryptophan biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00035; UER00040.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anthranilate synthase component 1 (EC:4.1.3.27)
    Short name:
    AS
    Short name:
    ASI
    Gene namesi
    Name:trpE
    OrganismiSerratia marcescens
    Taxonomic identifieri615 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 519519Anthranilate synthase component 1PRO_0000154111Add
    BLAST

    Proteomic databases

    PRIDEiP00897.

    Interactioni

    Subunit structurei

    Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    trpGP009002EBI-1031345,EBI-1031352

    Protein-protein interaction databases

    IntActiP00897. 1 interaction.

    Structurei

    Secondary structure

    1
    519
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi8 – 147
    Helixi20 – 289
    Beta strandi32 – 409
    Turni42 – 443
    Beta strandi47 – 6317
    Beta strandi66 – 716
    Helixi76 – 783
    Helixi79 – 868
    Beta strandi91 – 966
    Beta strandi99 – 1035
    Beta strandi109 – 1113
    Helixi113 – 1175
    Helixi123 – 1308
    Beta strandi132 – 1343
    Beta strandi142 – 1498
    Helixi151 – 1566
    Beta strandi171 – 18414
    Turni185 – 1884
    Beta strandi189 – 1968
    Helixi201 – 21818
    Beta strandi236 – 2394
    Helixi241 – 25616
    Beta strandi261 – 2633
    Beta strandi266 – 2727
    Helixi277 – 28610
    Beta strandi290 – 2967
    Beta strandi301 – 3077
    Beta strandi309 – 3146
    Turni315 – 3184
    Beta strandi319 – 3224
    Beta strandi325 – 3295
    Helixi341 – 35313
    Helixi355 – 37521
    Turni378 – 3803
    Beta strandi382 – 39110
    Beta strandi396 – 40611
    Helixi412 – 4198
    Helixi423 – 4253
    Beta strandi426 – 4294
    Helixi430 – 44112
    Turni446 – 4494
    Beta strandi450 – 4567
    Beta strandi461 – 4655
    Beta strandi468 – 4736
    Beta strandi476 – 4827
    Helixi491 – 51222

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1I7QX-ray1.95A/C1-519[»]
    1I7SX-ray2.40A/C1-519[»]
    ProteinModelPortaliP00897.
    SMRiP00897. Positions 3-519.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00897.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni290 – 2923Tryptophan binding
    Regioni327 – 3282Substrate binding
    Regioni482 – 4843Substrate binding

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.60.120.10. 1 hit.
    InterProiIPR005801. ADC_synthase.
    IPR019999. Anth_synth_I_like.
    IPR006805. Anth_synth_I_N.
    IPR005257. Anth_synth_I_TrpE.
    IPR015890. Chorismate-bd_C.
    [Graphical view]
    PfamiPF04715. Anth_synt_I_N. 1 hit.
    PF00425. Chorismate_bind. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001373. TrpE. 1 hit.
    PRINTSiPR00095. ANTSNTHASEI.
    SUPFAMiSSF56322. SSF56322. 1 hit.
    TIGRFAMsiTIGR00565. trpE_proteo. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P00897-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTKPQLTLL KVQASYRGDP TTLFHQLCGA RPATLLLESA EINDKQNLQS    50
    LLVIDSALPI TALGHTVSVQ ALTANGPALL PVLDEALPPE VRNQARPNGR 100
    ELTFPAIDAV QDEDARLRSL SVFDALRTLL TLVDSPADER EAVMLGGLFA 150
    YDLVAGFENL PAVRQDQRCP DFCFYLAETL LVLDHQRGSA RLQASVFSEQ 200
    ASEAQRLQHR LEQLQAELQQ PPQPIPHQKL ENMQLSCNQS DEEYGAVVSE 250
    LQEAIRQGEI FQVVPSRRFS LPCPAPLGPY QTLKDNNPSP YMFFMQDDDF 300
    TLFGASPESA LKYDAGNRQI EIYPIAGTRP RGRRADGSLD LDLDSRIELE 350
    MRTDHKELAE HLMLVDLARN DLARICQAGS RYVADLTKVD RYSFVMHLVS 400
    RVVGTLRADL DVLHAYQACM NMGTLSGAPK VRAMQLIAAL RSTRRGSYGG 450
    RVGYFTAHRH LDTCIVIRSA YVEDGHRTVQ AGAGVVQDSI RRREADETRN 500
    KARAVLRAIA TAHHAKEVF 519
    Length:519
    Mass (Da):57,658
    Last modified:August 14, 2001 - v2
    Checksum:iD3B59D47619FB0C1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti502 – 5043ARA → PVP in AAA57308. (PubMed:7007652)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY027546 Genomic DNA. Translation: AAA57308.2.
    PIRiA01117.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY027546 Genomic DNA. Translation: AAA57308.2 .
    PIRi A01117.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1I7Q X-ray 1.95 A/C 1-519 [» ]
    1I7S X-ray 2.40 A/C 1-519 [» ]
    ProteinModelPortali P00897.
    SMRi P00897. Positions 3-519.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P00897. 1 interaction.

    Proteomic databases

    PRIDEi P00897.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00035 ; UER00040 .

    Miscellaneous databases

    EvolutionaryTracei P00897.

    Family and domain databases

    Gene3Di 3.60.120.10. 1 hit.
    InterProi IPR005801. ADC_synthase.
    IPR019999. Anth_synth_I_like.
    IPR006805. Anth_synth_I_N.
    IPR005257. Anth_synth_I_TrpE.
    IPR015890. Chorismate-bd_C.
    [Graphical view ]
    Pfami PF04715. Anth_synt_I_N. 1 hit.
    PF00425. Chorismate_bind. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001373. TrpE. 1 hit.
    PRINTSi PR00095. ANTSNTHASEI.
    SUPFAMi SSF56322. SSF56322. 1 hit.
    TIGRFAMsi TIGR00565. trpE_proteo. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The structures of anthranilate synthase of Serratia marcescens crystallized in the presence of (i) its substrates, chorismate and glutamine, and a product, glutamate, and (ii) its end-product inhibitor, L-tryptophan."
      Spraggon G., Kim C., Nguyen-Huu X., Yee M.-C., Yanofsky C., Mills S.E.
      Proc. Natl. Acad. Sci. U.S.A. 98:6021-6026(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH SUBSTRATES AND MAGNESIUM ION, FUNCTION, COFACTOR, SUBUNIT.
    2. "Separation of anthranilate synthetase components I and II of Escherichia coli, Salmonella typhimurium, and Serratia marcescens and determination of their amino-terminal sequences by automatic Edman degradation."
      Li S.-L., Hanlon J., Yanofsky C.
      Biochemistry 13:1736-1744(1974) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-25.
      Strain: SM6.
    3. "The regulatory region of the trp operon of Serratia marcescens."
      Miozzari G.F., Yanofsky C.
      Nature 276:684-689(1978) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-33.
    4. "Nucleotide sequences of the trpG regions of Escherichia coli, Shigella dysenteriae, Salmonella typhimurium and Serratia marcescens."
      Nichols B.P., Miozzari G.F., van Cleemput M., Bennett G.N., Yanofsky C.
      J. Mol. Biol. 142:503-517(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 494-519.
      Strain: SM6.

    Entry informationi

    Entry nameiTRPE_SERMA
    AccessioniPrimary (citable) accession number: P00897
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: August 14, 2001
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3