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Protein

Anthranilate synthase component 1

Gene

trpE

Organism
Serratia marcescens
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia (Probable).1 Publication

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication

Enzyme regulationi

Feedback inhibited by tryptophan.By similarity

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase component 1 (trpE), Anthranilate synthase component 1 (trpE), Anthranilate synthase component 2 (trpG), Anthranilate synthase component 1 (trpE), Anthranilate synthase component 1 (trpE), Anthranilate synthase component 1 (AAY84_22430), Anthranilate synthase component 1 (AR325_16160), Anthranilate synthase component 1 (trpE), Anthranilate synthase component 1 (AYJ10_05620)
  2. Anthranilate phosphoribosyltransferase (trpGD), Anthranilate phosphoribosyltransferase (trpD), Anthranilate phosphoribosyltransferase (trpD), Anthranilate phosphoribosyltransferase (trpD), Anthranilate phosphoribosyltransferase (trpD), Anthranilate phosphoribosyltransferase (trpD), Anthranilate phosphoribosyltransferase (trpD2), Anthranilate phosphoribosyltransferase (trpD), Anthranilate phosphoribosyltransferase (trpD)
  3. N-(5'-phosphoribosyl)anthranilate isomerase (trpF), N-(5'-phosphoribosyl)anthranilate isomerase (trpF)
  4. Indole-3-glycerol phosphate synthase (AAY84_22415), Tryptophan biosynthesis protein TrpCF (trpC), Tryptophan biosynthesis protein TrpCF (trpC), Indole-3-glycerol phosphate synthase (AR325_16175), Bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase (AYJ10_05635), Tryptophan biosynthesis protein TrpCF (trpC), Tryptophan biosynthesis protein TrpCF (trpC)
  5. Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA), Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39TryptophanCombined sources1 Publication1
Metal bindingi360Magnesium1 Publication1
Binding sitei448Chorismate1 Publication1
Binding sitei468Chorismate1 Publication1
Binding sitei484Chorismate; via amide nitrogen1 Publication1
Metal bindingi497Magnesium1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi4.1.3.27. 5690.
UniPathwayiUPA00035; UER00040.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component 1 (EC:4.1.3.27)
Short name:
AS
Short name:
ASI
Gene namesi
Name:trpE
OrganismiSerratia marcescens
Taxonomic identifieri615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeSerratia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001541111 – 519Anthranilate synthase component 1Add BLAST519

Proteomic databases

PRIDEiP00897.

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
trpGP009002EBI-1031345,EBI-1031352

Protein-protein interaction databases

IntActiP00897. 1 interactor.

Structurei

Secondary structure

1519
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 14Combined sources7
Helixi20 – 28Combined sources9
Beta strandi32 – 40Combined sources9
Turni42 – 44Combined sources3
Beta strandi47 – 63Combined sources17
Beta strandi66 – 71Combined sources6
Helixi76 – 78Combined sources3
Helixi79 – 86Combined sources8
Beta strandi91 – 96Combined sources6
Beta strandi99 – 103Combined sources5
Beta strandi109 – 111Combined sources3
Helixi113 – 117Combined sources5
Helixi123 – 130Combined sources8
Beta strandi132 – 134Combined sources3
Beta strandi142 – 149Combined sources8
Helixi151 – 156Combined sources6
Beta strandi171 – 184Combined sources14
Turni185 – 188Combined sources4
Beta strandi189 – 196Combined sources8
Helixi201 – 218Combined sources18
Beta strandi236 – 239Combined sources4
Helixi241 – 256Combined sources16
Beta strandi261 – 263Combined sources3
Beta strandi266 – 272Combined sources7
Helixi277 – 286Combined sources10
Beta strandi290 – 296Combined sources7
Beta strandi301 – 307Combined sources7
Beta strandi309 – 314Combined sources6
Turni315 – 318Combined sources4
Beta strandi319 – 322Combined sources4
Beta strandi325 – 329Combined sources5
Helixi341 – 353Combined sources13
Helixi355 – 375Combined sources21
Turni378 – 380Combined sources3
Beta strandi382 – 391Combined sources10
Beta strandi396 – 406Combined sources11
Helixi412 – 419Combined sources8
Helixi423 – 425Combined sources3
Beta strandi426 – 429Combined sources4
Helixi430 – 441Combined sources12
Turni446 – 449Combined sources4
Beta strandi450 – 456Combined sources7
Beta strandi461 – 465Combined sources5
Beta strandi468 – 473Combined sources6
Beta strandi476 – 482Combined sources7
Helixi491 – 512Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I7QX-ray1.95A/C1-519[»]
1I7SX-ray2.40A/C1-519[»]
ProteinModelPortaliP00897.
SMRiP00897.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00897.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni290 – 292Tryptophan bindingCombined sources1 Publication3
Regioni327 – 328Chorismate binding1 Publication2
Regioni482 – 484Chorismate binding1 Publication3

Sequence similaritiesi

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR005257. Anth_synth_I_TrpE.
IPR015890. Chorismate_C.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PIRSFiPIRSF001373. TrpE. 1 hit.
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR00565. trpE_proteo. 1 hit.

Sequencei

Sequence statusi: Complete.

P00897-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTKPQLTLL KVQASYRGDP TTLFHQLCGA RPATLLLESA EINDKQNLQS
60 70 80 90 100
LLVIDSALPI TALGHTVSVQ ALTANGPALL PVLDEALPPE VRNQARPNGR
110 120 130 140 150
ELTFPAIDAV QDEDARLRSL SVFDALRTLL TLVDSPADER EAVMLGGLFA
160 170 180 190 200
YDLVAGFENL PAVRQDQRCP DFCFYLAETL LVLDHQRGSA RLQASVFSEQ
210 220 230 240 250
ASEAQRLQHR LEQLQAELQQ PPQPIPHQKL ENMQLSCNQS DEEYGAVVSE
260 270 280 290 300
LQEAIRQGEI FQVVPSRRFS LPCPAPLGPY QTLKDNNPSP YMFFMQDDDF
310 320 330 340 350
TLFGASPESA LKYDAGNRQI EIYPIAGTRP RGRRADGSLD LDLDSRIELE
360 370 380 390 400
MRTDHKELAE HLMLVDLARN DLARICQAGS RYVADLTKVD RYSFVMHLVS
410 420 430 440 450
RVVGTLRADL DVLHAYQACM NMGTLSGAPK VRAMQLIAAL RSTRRGSYGG
460 470 480 490 500
RVGYFTAHRH LDTCIVIRSA YVEDGHRTVQ AGAGVVQDSI RRREADETRN
510
KARAVLRAIA TAHHAKEVF
Length:519
Mass (Da):57,658
Last modified:August 14, 2001 - v2
Checksum:iD3B59D47619FB0C1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti502 – 504ARA → PVP in AAA57308 (PubMed:7007652).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY027546 Genomic DNA. Translation: AAA57308.2.
PIRiA01117.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY027546 Genomic DNA. Translation: AAA57308.2.
PIRiA01117.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I7QX-ray1.95A/C1-519[»]
1I7SX-ray2.40A/C1-519[»]
ProteinModelPortaliP00897.
SMRiP00897.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP00897. 1 interactor.

Proteomic databases

PRIDEiP00897.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
BRENDAi4.1.3.27. 5690.

Miscellaneous databases

EvolutionaryTraceiP00897.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR005257. Anth_synth_I_TrpE.
IPR015890. Chorismate_C.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PIRSFiPIRSF001373. TrpE. 1 hit.
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR00565. trpE_proteo. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRPE_SERMA
AccessioniPrimary (citable) accession number: P00897
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 14, 2001
Last modified: November 2, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.