Reviewed, UniProtKB/Swiss-Prot P00895 (TRPE_ECOLI)

Names and origin

Protein names

Recommended name:
    Anthranilate synthase component 1
    EC=4.1.3.27
Alternative name(s):
    Anthranilate synthase component I

Gene names

Name:	trpE
Ordered Locus Names:	b1264, JW1256

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Tetramer of two components I and two components II.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Belongs to the anthranilate synthase component I family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 520	520	Anthranilate synthase component 1		PRO_0000154092
Natural variations
	Natural variant	13	1	C → S in strain: TRPA2/COLVB.
Experimental info
	Sequence conflict	147	1	G → S Ref.1
	Sequence conflict	147	1	G → S Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P00895-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 87696C0538CF66EA	FASTA	520	57,494
	10 20 30 40 50 60 MQTQKPTLEL LTCEGAYRDN PTALFHQLCG DRPATLLLES ADIDSKDDLK SLLLVDSALR 70 80 90 100 110 120 ITALGDTVTI QALSGNGEAL LALLDNALPA GVESEQSPNC RVLRFPPVSP LLDEDARLCS 130 140 150 160 170 180 LSVFDAFRLL QNLLNVPKEE REAMFFGGLF SYDLVAGFED LPQLSAENNC PDFCFYLAET 190 200 210 220 230 240 LMVIDHQKKS TRIQASLFAP NEEEKQRLTA RLNELRQQLT EAAPPLPVVS VPHMRCECNQ 250 260 270 280 290 300 SDEEFGGVVR LLQKAIRAGE IFQVVPSRRF SLPCPSPLAA YYVLKKSNPS PYMFFMQDND 310 320 330 340 350 360 FTLFGASPES SLKYDATSRQ IEIYPIAGTR PRGRRADGSL DRDLDSRIEL EMRTDHKELS 370 380 390 400 410 420 EHLMLVDLAR NDLARICTPG SRYVADLTKV DRYSYVMHLV SRVVGELRHD LDALHAYRAC 430 440 450 460 470 480 MNMGTLSGAP KVRAMQLIAE AEGRRRGSYG GAVGYFTAHG DLDTCIVIRS ALVENGIATV 490 500 510 520 QAGAGVVLDS VPQSEADETR NKARAVLRAI ATAHHAQETF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete nucleotide sequence of the tryptophan operon of Escherichia coli." Yanofsky C., Platt T., Crawford I.P., Nichols B.P., Christie G.E., Horowitz H., van Cleemput M., Wu A.M. Nucleic Acids Res. 9:6647-6668(1981) [PubMed: 7038627] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of Escherichia coli trpE. Anthranilate synthetase component I contains no tryptophan residues." Nichols B.P., van Cleemput M., Yanofsky C. J. Mol. Biol. 146:45-54(1981) [PubMed: 7021857] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Comparison of the nucleotide sequences of the initial transcribed regions of the tryptophan operons of Escherichia coli and Salmonella typhimurium." Lee F., Bertrand K., Bennett G.N., Yanofsky C. J. Mol. Biol. 121:193-217(1978) [PubMed: 351195] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-33.
[7]	"Separation of anthranilate synthetase components I and II of Escherichia coli, Salmonella typhimurium, and Serratia marcescens and determination of their amino-terminal sequences by automatic Edman degradation." Li S.-L., Hanlon J., Yanofsky C. Biochemistry 13:1736-1744(1974) [PubMed: 4598537] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-25. Strain: TRPA2/COLVB.

Cross-references

Sequence databases
EMBLGenBankDDBJ	V00368 Genomic DNA. Translation: CAA23666.1. V00372 Genomic DNA. Translation: CAA23671.1. J01714 Genomic DNA. Translation: AAA57297.1. U00096 Genomic DNA. Translation: AAC74346.1. AP009048 Genomic DNA. Translation: BAA14799.1. M24959 Genomic DNA. Translation: AAA24692.1.
PIR	NNEC1. C64874.
RefSeq	AP_001890.1. NP_415780.1.
3D structure databases
HSSP	HSSP built from PDB template 1I1Q based on UniProtKB P00898.
SMR	P00895. Positions 5-516.
ModBase	Search...
Protein-protein interaction databases
STRING	P00895.
2-D gel databases
ECO2DBASE	E050.0. 6TH EDITION.
Genome annotation databases
GeneID	945846.
GenomeReviews	Gene locus JW1256 in contig AP009048_GR. Gene locus b1264 in contig U00096_GR.
KEGG	ecj:JW1256. eco:b1264.
Organism-specific databases
EchoBASE	EB1021.
EcoGene	EG11028. trpE.
CMR	Search...
Phylogenomic databases
HOGENOM	P00895.
OMA	HKELAEH.
Enzyme and pathway databases
BioCyc	EcoCyc:ANTHRANSYNCOMPI-MON.
Gene expression databases
Genevestigator	P00895.
Family and domain databases
InterPro	IPR005801. ADC_synthase. IPR006805. Anth_synth_I_N. IPR019999. Anthranilate_synth_I_C. IPR015890. Chorismate-bd_C. IPR005257. TrpE_synth. [Graphical view]
Gene3D	G3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit.
PANTHER	PTHR11236. TRPE_1_chor_bd. 1 hit.
Pfam	PF04715. Anth_synt_I_N. 1 hit. PF00425. Chorismate_bind. 1 hit. [Graphical view]
PRINTS	PR00095. ANTSNTHASEI.
ProDom	PD000779. Anth_synth_chor. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00565. trpE_proteo. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRPE_ECOLI

Accession

Primary (citable) accession number: P00895
Secondary accession number(s): P78249

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 1, 1997
Last modified:	November 3, 2009
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)