P00894 (ILVH_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetolactate synthase isozyme 3 small subunit
EC=2.2.1.6

Alternative name(s):
ALS-III
Acetohydroxy-acid synthase III small subunit
Short name=AHAS-III

Gene names

Name:	ilvH
Synonyms:	brnP
Ordered Locus Names:	b0078, JW0077

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	163 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	2 pyruvate = 2-acetolactate + CO2.
Enzyme regulation	Sensitive to valine inhibition.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.
Subunit structure	Dimer of large and small chains.
Miscellaneous	E.coli contains genes for 3 AHAS isozymes: ilvBN, ilvGM and ilvIH.
Sequence similarities	Belongs to the acetolactate synthase small subunit family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Molecular function	Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from direct assay. Source: EcoCyc valine biosynthetic process Inferred from direct assay. Source: EcoCyc
Molecular function	acetolactate synthase activity Inferred from direct assay. Source: EcoCyc amino acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 163

163

Acetolactate synthase isozyme 3 small subunit

PRO_0000151410

Regions

Domain

3 – 76

74

ACT

Experimental info

Sequence conflict

132

1

D → S Ref.1

Sequence conflict

132

1

D → S Ref.2

Sequence conflict

132

1

D → S Ref.5

Sequence conflict

159 – 163

5

DKIMR → VK in CAA25756. Ref.1

Secondary structure

1

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163

Helix Strand Turn

Details...

Beta strand

2 – 10

9

Helix

15 – 24

10

Turn

25 – 27

3

Beta strand

31 – 37

7

Beta strand

41 – 52

12

Helix

54 – 66

13

Beta strand

70 – 75

6

Helix

76 – 78

3

Beta strand

81 – 92

12

Helix

96 – 107

12

Beta strand

111 – 115

5

Beta strand

117 – 126

10

Helix

128 – 138

11

Turn

139 – 141

3

Beta strand

142 – 149

8

Beta strand

153 – 157

5

Sequences

	Sequence		Length	Mass (Da)
	P00894 [UniParc]. Last modified July 19, 2003. Version 3. Checksum: D6E28AE94A55606E	FASTA	163	17,977
	10 20 30 40 50 60 MRRILSVLLE NESGALSRVI GLFSQRGYNI ESLTVAPTDD PTLSRMTIQT VGDEKVLEQI 70 80 90 100 110 120 EKQLHKLVDV LRVSELGQGA HVEREIMLVK IQASGYGRDE VKRNTEIFRG QIIDVTPSLY 130 140 150 160 TVQLAGTSGK LDAFLASIRD VAKIVEVARS GVVGLSRGDK IMR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular structure of ilvIH and its evolutionary relationship to ilvG in Escherichia coli K12." Squires C.H., Defelice M., Devereux J., Calvo J.M. Nucleic Acids Res. 11:5299-5313(1983) [PubMed: 6308579] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 132. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Nucleotide sequence of the ilvH-fruR gene region of Escherichia coli K12 and Salmonella typhimurium LT2." Jahreis K., Postma P.W., Lengeler J.W. Mol. Gen. Genet. 226:332-336(1991) [PubMed: 1851954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 130-163. Strain: K12.
[6]	"Molecular cloning, nucleotide sequence, and expression of shl, a new gene in the 2-minute region of the genetic map of Escherichia coli." Leclerc G., Noel G., Drapeau G.R. J. Bacteriol. 172:4696-4700(1990) [PubMed: 2198273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 130-163.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X01609 Genomic DNA. Translation: CAA25756.1.
X55457 Genomic DNA. No translation available.
X55034 Genomic DNA. Translation: CAA38855.1.
U00096 Genomic DNA. Translation: AAC73189.1.
AP009048 Genomic DNA. Translation: BAB96647.2.
M35034 Genomic DNA. Translation: AAA24627.1.

PIR

YCEC3H. F64729.

RefSeq

NP_414620.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2F1F	X-ray	1.75	A/B	2-163	[»]

ProteinModelPortal

P00894.

SMR

P00894. Positions 2-163.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10024N.

2D gel databases

SWISS-2DPAGE

P00894.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000002562; EBESCP00000002562; EBESCG00000002091.
EBESCT00000002563; EBESCP00000002563; EBESCG00000002091.
EBESCT00000002564; EBESCP00000002564; EBESCG00000002091.
EBESCT00000002565; EBESCP00000002565; EBESCG00000002091.
EBESCT00000015938; EBESCP00000015229; EBESCG00000014998.

GeneID

947267.

GenomeReviews

Gene locus JW0077 in contig AP009048_GR.
Gene locus b0078 in contig U00096_GR.

KEGG

ecj:JW0077.
eco:b0078.

PATRIC

32115259. VBIEscCol129921_0081.

Organism-specific databases

EchoBASE

EB0494.

EcoGene

EG10499. ilvH.

Phylogenomic databases

eggNOG

COG0440.

GeneTree

EBGT00050000011172.

HOGENOM

HBG335310.

OMA

IERELAM.

PhylomeDB

P00894.

ProtClustDB

PRK11895.

Enzyme and pathway databases

BioCyc

EcoCyc:ACETOLACTSYNIII-HCHAIN-MONOMER.
MetaCyc:ACETOLACTSYNIII-HCHAIN-MONOMER.

Gene expression databases

Genevestigator

P00894.

Family and domain databases

InterPro

IPR004789. Acetalactate_synth_ssu.
IPR019455. Acetolactate_synth_ssu_C.
IPR002912. ACT-bd.
[Graphical view]

KO

K01653.

Pfam

PF01842. ACT. 1 hit.
PF10369. ALS_ss_C. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00119. Acolac_sm. 1 hit.

ProtoNet

Search...

Entry information

Entry name

ILVH_ECOLI

Accession

Primary (citable) accession number: P00894
Secondary accession number(s): P78046, Q47637

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 19, 2003
Last modified:	January 25, 2012
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program